Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,790,919 A→C L143V (TTA→GTA)  tdh ← L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,790,9190AC98.7% 827.9 / ‑1.7 235L143V (TTA→GTA) tdhL‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base C (123/108);  total (125/110)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 235 total aligned reads displayed.

ATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTA  >  NC_000913/3790822‑3791014
                                                                                                 |                                                                                               
  cGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCa                                                                                             >  2:1765887/1‑100 (MQ=255)
    ggCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATc                                                                                           >  2:830434/1‑100 (MQ=255)
     gCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCg                                                                                          <  2:2592602/100‑1 (MQ=255)
       cTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGa                                                                                        >  1:3637091/1‑100 (MQ=255)
          cACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAat                                                                                     >  1:4816968/1‑100 (MQ=255)
          cACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAat                                                                                     <  1:1428008/100‑1 (MQ=255)
           aCCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAata                                                                                    >  2:541304/1‑100 (MQ=255)
           aCCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAata                                                                                    <  2:50250/100‑1 (MQ=255)
           aCCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAata                                                                                    >  1:3511313/1‑100 (MQ=255)
            ccAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAatat                                                                                   >  1:1968651/1‑100 (MQ=255)
              aGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTg                                                                                 >  1:128853/1‑100 (MQ=255)
              aGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTg                                                                                 >  2:2258465/1‑100 (MQ=255)
               gAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGt                                                                                >  1:3963888/1‑100 (MQ=255)
                 aaCCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCg                                                                              <  1:260228/100‑1 (MQ=255)
                     aGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGa                                                                          <  1:2791504/100‑1 (MQ=255)
                     aGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGa                                                                          >  1:2384733/1‑100 (MQ=255)
                     aGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGa                                                                          >  2:1840268/1‑100 (MQ=255)
                      gCACCTCCTCGCCCCCCAGATCAAACGACAGAGCGGTATGCACGCCGTTACCGAAGGGATCAAAAATTGAGGCTACGTCGTCGGAAATATTGTAGGGGac                                                                         <  2:1370945/100‑2 (MQ=255)
                      gCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGan                                                                         <  1:4997355/100‑2 (MQ=255)
                        acaTCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGAttt                                                                       >  1:381617/1‑100 (MQ=255)
                         caTCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGAtttt                                                                      >  2:3142609/1‑100 (MQ=255)
                         caTCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGAtttt                                                                      >  1:3462809/1‑100 (MQ=255)
                          aTCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTg                                                                     <  1:2278474/100‑1 (MQ=255)
                          aTCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTg                                                                     >  2:4899452/1‑100 (MQ=255)
                          aTCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCAAAAGGATCAAAAATTGCGGCTACGTCATCTGAAATATTGTCGGGGATTTTg                                                                     <  2:2769645/100‑1 (MQ=255)
                           tCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGa                                                                    >  2:4876670/1‑100 (MQ=255)
                            cTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGaa                                                                   >  1:3775575/1‑100 (MQ=255)
                              tCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAgg                                                                 <  2:461969/100‑1 (MQ=255)
                                 cccACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGt                                                              >  1:3267416/1‑100 (MQ=255)
                                   caccaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGTAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTg                                                            <  1:2557221/100‑1 (MQ=255)
                                   caccaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTg                                                            <  2:1805964/100‑1 (MQ=255)
                                   caccaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTg                                                            >  1:5145284/1‑100 (MQ=255)
                                   caccaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTg                                                            <  1:2236513/100‑1 (MQ=255)
                                   caccaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTg                                                            >  2:923606/1‑100 (MQ=255)
                                   caccaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTg                                                            <  1:1208208/100‑1 (MQ=255)
                                   caccaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGCCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTg                                                            <  2:1053067/100‑1 (MQ=255)
                                     ccaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGaa                                                          <  2:1541553/100‑1 (MQ=255)
                                      caGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAAt                                                         <  2:2147820/100‑1 (MQ=255)
                                        gATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGc                                                       >  1:4322557/1‑100 (MQ=255)
                                         aTCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGcc                                                      >  2:2960603/1‑100 (MQ=255)
                                         aTCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGcc                                                      >  2:1154877/1‑100 (MQ=255)
                                         aTCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGcc                                                      >  1:1156506/1‑100 (MQ=255)
                                          tCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCg                                                     <  1:715883/100‑1 (MQ=255)
                                          gatctcGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCg                                                     <  1:809981/95‑1 (MQ=255)
                                           cAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCgg                                                    >  2:3926613/1‑100 (MQ=255)
                                           aaaaaGACAGCGCGGTATGCACGGCGTTAAAAAAAAAAAAAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCgg                                                    <  2:4570882/99‑1 (MQ=255)
                                            naaCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCggg                                                   >  1:4449126/2‑100 (MQ=255)
                                            aaaCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCggg                                                   >  2:2232087/1‑100 (MQ=255)
                                            aaaCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCggg                                                   >  2:2058410/1‑100 (MQ=255)
                                            aaaCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCggg                                                   >  2:1918460/1‑100 (MQ=255)
                                             aaCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGa                                                  >  2:1282584/1‑100 (MQ=255)
                                              aCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGAt                                                 >  2:2749009/1‑100 (MQ=255)
                                               cGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGAGATc                                                >  2:799503/1‑100 (MQ=255)
                                                  cAGCGGGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCAcc                                             <  2:347831/100‑1 (MQ=255)
                                                  cAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCAcc                                             <  1:3263369/100‑1 (MQ=255)
                                                    gcgcGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATAGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAg                                           >  1:3594200/1‑100 (MQ=255)
                                                    gcgcGGTATGCACGGCGTTACCGAAGGGATCAAAAACTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAg                                           <  1:5242169/100‑1 (MQ=255)
                                                       cgGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGata                                        >  2:96930/1‑100 (MQ=255)
                                                       cgGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGata                                        <  1:4580549/100‑1 (MQ=255)
                                                        ggTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGatat                                       <  2:473777/100‑1 (MQ=255)
                                                         gTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATAtt                                      >  1:59611/1‑100 (MQ=255)
                                                         gTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATAtt                                      <  2:5256626/100‑1 (MQ=255)
                                                          tATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTc                                     >  2:310933/1‑100 (MQ=255)
                                                          tATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTc                                     <  1:2302626/100‑1 (MQ=255)
                                                             gCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGc                                  <  1:2748690/100‑1 (MQ=255)
                                                             gCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGc                                  >  2:1206210/1‑100 (MQ=255)
                                                             gCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGc                                  >  1:1690975/1‑100 (MQ=255)
                                                             gCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGc                                  <  2:246083/100‑1 (MQ=255)
                                                             gCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGc                                  <  1:1872119/100‑1 (MQ=255)
                                                              cACGGCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCa                                 >  2:3996783/1‑100 (MQ=255)
                                                                  gCGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAagc                             <  1:896658/100‑1 (MQ=255)
                                                                   cGTTACCGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAagca                            <  2:4167863/100‑1 (MQ=255)
                                                                       aCCGCAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGccc                        >  2:4647806/1‑100 (MQ=255)
                                                                        ccGAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCg                       >  1:3383648/1‑100 (MQ=255)
                                                                          gAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCggg                     >  1:3994807/1‑100 (MQ=255)
                                                                          gAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCggg                     >  1:5407680/1‑100 (MQ=255)
                                                                          gAAGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTCGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCggg                     >  1:5015943/1‑100 (MQ=255)
                                                                           aaGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGc                    <  2:4406839/100‑1 (MQ=255)
                                                                            aGGGATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCg                   <  2:3258805/100‑1 (MQ=255)
                                                                             gggATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGa                  <  2:2918900/100‑1 (MQ=255)
                                                                              ggATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGAt                 <  1:1484983/100‑1 (MQ=255)
                                                                               gATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGAtt                >  1:1666237/1‑100 (MQ=255)
                                                                               gATCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGAtt                <  2:3740912/100‑1 (MQ=255)
                                                                                aTCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTa               >  1:2040351/1‑100 (MQ=255)
                                                                                aTCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTa               >  1:1596718/1‑100 (MQ=255)
                                                                                aTCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTa               <  1:4051462/100‑1 (MQ=255)
                                                                                aTCAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGACGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGAttg               >  1:5570152/1‑99 (MQ=255)
                                                                                  cAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAac             >  1:705050/1‑100 (MQ=255)
                                                                                  cAAAAATTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGAGTAac             >  1:3058050/1‑100 (MQ=255)
                                                                                   aaaaaTTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCAGCAGATATTCGGCAAAGCAGCCCGGGCGATTAaca            >  2:5246254/1‑100 (MQ=255)
                                                                                   aaaaaTTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAaca            >  1:4601613/1‑100 (MQ=255)
                                                                                   aaaaaTTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAaca            >  1:4270933/1‑100 (MQ=255)
                                                                                   aaaaaTTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAaca            >  1:5193379/1‑100 (MQ=255)
                                                                                   aaaaaTTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAaca            >  2:3867194/1‑100 (MQ=255)
                                                                                    aaaaTTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAacac           <  1:4968911/100‑1 (MQ=255)
                                                                                     aaaTTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACAcc          <  1:102274/100‑1 (MQ=255)
                                                                                       aTTGCGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCaa        <  2:696400/100‑1 (MQ=255)
                                                                                           cGGCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGcc    <  2:1498373/100‑1 (MQ=255)
                                                                                            ggCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCt   >  2:2882532/1‑100 (MQ=255)
                                                                                             gCTACGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTa  >  1:4682168/1‑100 (MQ=255)
                                                                                                 |                                                                                               
ATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTA  >  NC_000913/3790822‑3791014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑