Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,807,410 T→G K230T (AAA→ACA)  waaQ ← lipopolysaccharide core biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,807,4100TG99.5% 742.7 / ‑5.1 209K230T (AAA→ACA) waaQlipopolysaccharide core biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (104/104);  total (104/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 211 total aligned reads displayed.

CCCGGCGCAGAATCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTTTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGCAGCG  >  NC_000913/3807314‑3807506
                                                                                                |                                                                                                
cccGGCGCAGAATCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTg                                                                                               >  2:1811790/1‑100 (MQ=255)
cccGGCGCAGAATCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTg                                                                                               >  2:4648256/1‑100 (MQ=255)
 ccGGCGCAGAATCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGtgttgt                                                                                              <  2:1698682/100‑1 (MQ=255)
     cgcAGAATCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCg                                                                                          >  2:3466610/1‑100 (MQ=255)
      gcAGAATCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGc                                                                                         >  2:3736164/1‑100 (MQ=255)
       cAGAATCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCa                                                                                        >  2:3184944/1‑100 (MQ=255)
        aGAATCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCaa                                                                                       >  2:4403750/1‑100 (MQ=255)
         naaTCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAAc                                                                                      >  2:1425534/2‑100 (MQ=255)
         gAATCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAAc                                                                                      <  1:5023069/100‑1 (MQ=255)
         gAATCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAAc                                                                                      >  1:4007078/1‑100 (MQ=255)
          aaTCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAAcc                                                                                     <  1:1811742/100‑1 (MQ=255)
           aTCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCt                                                                                    <  1:4898692/100‑1 (MQ=255)
              cacaCGAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAAcgtcgt                                                                                 >  1:4992999/1‑100 (MQ=255)
              cacaCCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGt                                                                                 <  2:2926018/100‑1 (MQ=255)
              cacaCCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGt                                                                                 >  2:1151409/1‑100 (MQ=255)
              cacaCCAATAAAAAGCACTGCATGATCAATTAATGCAACCAGTTCAGGAAGACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGt                                                                                 >  1:242016/1‑100 (MQ=255)
               acacCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTg                                                                                <  1:454495/100‑1 (MQ=255)
                  ccAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCaa                                                                             >  1:3977208/1‑100 (MQ=255)
                  acAATAAAAAGCAATGCATGATCACTTAATGCACCCAGTTCAGGAAGACGTGTTTTACCTGCAAGCCCAGTAATCGGAGATGTTTCTCAACCTCGTGCaa                                                                             >  2:779697/2‑100 (MQ=255)
                   cgaTAAAAAGCACTGCATGATCAATTAATGCATCCAGTTCGGGAAAACGTGCTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAAt                                                                            <  2:4457280/98‑1 (MQ=255)
                   cAATAAAAAGCACTGCATGATCAATTAATGCACCAAGTTCAGGAAAACGTGTTATACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAAt                                                                            >  2:5078032/1‑100 (MQ=255)
                    aaTAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAAtc                                                                           >  1:2916007/1‑100 (MQ=255)
                     aTAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAAtct                                                                          <  1:2991606/100‑1 (MQ=255)
                     aTAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAAtct                                                                          >  1:2413295/1‑100 (MQ=255)
                      tAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAAtctc                                                                         >  2:3903607/1‑100 (MQ=255)
                       aaaaaGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTTGCAACCTCGTGCAATCTCa                                                                        >  2:322327/1‑100 (MQ=255)
                       aaaaaGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCa                                                                        <  2:2471901/100‑1 (MQ=255)
                        aaaaGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCAt                                                                       >  2:4876229/1‑100 (MQ=255)
                         aaaGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATc                                                                      <  2:5309761/100‑1 (MQ=255)
                          aaGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCt                                                                     <  2:3549877/100‑1 (MQ=255)
                            gCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTac                                                                   >  2:3854426/1‑100 (MQ=255)
                             cACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTaca                                                                  <  1:4783817/100‑1 (MQ=255)
                               cTGCATGCTCAATTAATGCACAAAAAAAAAAAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTAcaca                                                                <  2:2710171/100‑1 (MQ=255)
                               cTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTAcaca                                                                >  2:1673793/1‑100 (MQ=255)
                               cTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTAcaca                                                                <  2:2418463/100‑1 (MQ=255)
                                 gCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAg                                                              >  1:2975183/1‑100 (MQ=255)
                                 gCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAg                                                              >  1:2223022/1‑100 (MQ=255)
                                    tGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCga                                                           >  2:3989475/1‑100 (MQ=255)
                                     gATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCgag                                                          >  1:3592873/1‑100 (MQ=255)
                                     gATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCgag                                                          >  1:2810065/1‑100 (MQ=255)
                                     gATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCgag                                                          >  1:830909/1‑100 (MQ=255)
                                     gATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCgag                                                          >  2:2124111/1‑100 (MQ=255)
                                      aTCAATTAATGCACCCAGTTCAGGACAACGAGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCgagc                                                         >  2:284917/1‑99 (MQ=255)
                                      aTCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCgaga                                                         <  1:2640738/100‑1 (MQ=255)
                                      aTCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCgaga                                                         <  2:3126218/100‑1 (MQ=255)
                                       tCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGat                                                        <  1:693095/100‑1 (MQ=255)
                                         aaTTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGatca                                                      >  2:4083119/1‑100 (MQ=255)
                                           tgaaTGCACCCATTTCATGAAAACGTGGTGTACCTGCAAGGCCAGTAATGGGGTTTGTTTCGCAACCGCGGTCAATCTCATCTACACAAGCGAGatcagc                                                    <  1:5300531/98‑3 (MQ=255)
                                             aaTGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTg                                                  >  1:5199856/1‑100 (MQ=255)
                                             aaTGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTg                                                  >  2:1898409/1‑100 (MQ=255)
                                                gCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGTTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCg                                               <  2:1083584/100‑1 (MQ=255)
                                                 cACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCACTAATGGGCGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGa                                              >  1:4955095/1‑100 (MQ=255)
                                                  aCCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAg                                             >  1:878926/1‑100 (MQ=255)
                                                    ccAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAggg                                           <  1:2075801/100‑1 (MQ=255)
                                                    ccAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAggg                                           <  1:2624687/100‑1 (MQ=255)
                                                     cAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGc                                          >  2:2327484/1‑100 (MQ=255)
                                                     cAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGc                                          <  1:1112733/100‑1 (MQ=255)
                                                      aGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGcc                                         <  1:1693658/100‑1 (MQ=255)
                                                      aGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGcc                                         <  1:3211413/100‑1 (MQ=255)
                                                        ttCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCca                                       <  1:2192060/100‑1 (MQ=255)
                                                         tCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCcac                                      <  1:4153708/100‑1 (MQ=255)
                                                          cAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCcaca                                     >  2:1366075/1‑100 (MQ=255)
                                                            ggAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAgg                                   >  1:1245732/1‑100 (MQ=255)
                                                             gAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGt                                  >  1:868058/1‑100 (MQ=255)
                                                              aaaaCGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGtt                                 <  1:3537150/100‑1 (MQ=255)
                                                                aaCGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAg                               <  1:1285607/100‑1 (MQ=255)
                                                                aaCGTGTTTTACCTGCAAGGCCAGTAATGGGGGGTGTTTCGCGACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAg                               <  2:1070032/100‑1 (MQ=255)
                                                                 aCGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGc                              <  2:419893/100‑1 (MQ=255)
                                                                  cGTGTTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCACAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGca                             <  1:4074752/100‑1 (MQ=255)
                                                                   gtgtTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGcac                            <  1:4320807/100‑1 (MQ=255)
                                                                     gtTTTACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACaa                          >  1:1507128/1‑100 (MQ=255)
                                                                      ttttACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAAc                         >  1:3124571/1‑100 (MQ=255)
                                                                         tACCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGCTCATCTGCCGAGGGCCCACAGGTTAGCACAACCTg                      >  2:4556079/1‑100 (MQ=255)
                                                                          aCCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGa                     >  2:2524210/1‑100 (MQ=255)
                                                                          aCCTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGa                     >  2:1271913/1‑100 (MQ=255)
                                                                           ccTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGAt                    >  2:2634070/1‑100 (MQ=255)
                                                                           ccTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGAt                    <  2:2940126/100‑1 (MQ=255)
                                                                           ccTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGAt                    <  2:2277405/100‑1 (MQ=255)
                                                                            cTGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATa                   >  2:1549174/1‑100 (MQ=255)
                                                                             tGCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAg                  <  1:3889994/100‑1 (MQ=255)
                                                                              gCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGc                 <  2:2260535/100‑1 (MQ=255)
                                                                              gCAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGc                 >  1:2306013/1‑100 (MQ=255)
                                                                               cAAGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGcc                >  2:4981774/1‑100 (MQ=255)
                                                                                aaGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGccn               <  1:1444370/100‑2 (MQ=255)
                                                                                aaGGCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCt               <  1:5051798/100‑1 (MQ=255)
                                                                                  ggCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCg             >  2:652763/1‑100 (MQ=255)
                                                                                   gCCAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGc            >  2:1308145/1‑100 (MQ=255)
                                                                                     cAGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTg          >  2:3156917/1‑100 (MQ=255)
                                                                                      aGTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGt         <  1:1015037/100‑1 (MQ=255)
                                                                                       gTAATGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGtt        >  1:472996/1‑100 (MQ=255)
                                                                                         aaTGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGc      <  1:2140211/100‑1 (MQ=255)
                                                                                         aaTGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGc      <  2:3830169/100‑1 (MQ=255)
                                                                                         aaTGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGc      <  1:1511958/100‑1 (MQ=255)
                                                                                         aaTGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGc      <  2:1488026/100‑1 (MQ=255)
                                                                                           tGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGCAg    >  1:3291839/1‑100 (MQ=255)
                                                                                           tGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGCAg    <  1:3762712/100‑1 (MQ=255)
                                                                                           tGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGCAg    <  1:4837631/100‑1 (MQ=255)
                                                                                           tGGGTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGCAg    >  1:1211171/1‑100 (MQ=255)
                                                                                            gggTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGCAgc   >  1:5488075/1‑100 (MQ=255)
                                                                                             ggTGTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGCAgcg  >  1:42078/1‑100 (MQ=255)
                                                                                                |                                                                                                
CCCGGCGCAGAATCCACACCAATAAAAAGCACTGCATGATCAATTAATGCACCCAGTTCAGGAAAACGTGTTTTACCTGCAAGGCCAGTAATGGGTTTTGTTTCGCAACCTCGTGCAATCTCATCTACACAAGCGAGATCATCTGCCGAGGGCCCACAGGTTAGCACAACCTGATAGCCTCGCTGTTGCAGCG  >  NC_000913/3807314‑3807506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: