Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 25,474 T→G S90A (TCG→GCG)  lspA → prolipoprotein signal peptidase (signal peptidase II)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091325,4740TG97.3% 783.8 / 1.8 227S90A (TCG→GCG) lspAprolipoprotein signal peptidase (signal peptidase II)
Reads supporting (aligned to +/- strand):  ref base T (4/2);  new base G (111/108);  total (117/110)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

GCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTC  >  NC_000913/25375‑25572
                                                                                                   |                                                                                                  
gcgTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCGGGCAGTGATGATGTATCGCt                                                                                                    <  2:1810436/100‑1 (MQ=255)
    ttAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGaa                                                                                                <  1:1559809/100‑1 (MQ=255)
     tAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAg                                                                                               <  1:5184975/100‑1 (MQ=255)
     tAGTTTCCTTGCCGATAGCGGCGGCTGACAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAg                                                                                               <  2:5380988/100‑1 (MQ=255)
      aGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAgg                                                                                              <  2:290886/100‑1 (MQ=255)
       gTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGc                                                                                             >  1:91777/1‑100 (MQ=255)
        tttCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGcc                                                                                            >  1:2053745/1‑100 (MQ=255)
          tCCTTGCCGATAGCGGCGGCTTGCAGCCTTGGTTCTTTGCCGGTATTGCAATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCAc                                                                                          <  2:4075982/100‑1 (MQ=255)
            cTTGCCGATAGCCGCGGCTGGCAGCGTTCGTGCTTTGCCGGTATTGCGATTGGTATTAGCGGGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGc                                                                                        <  2:699919/100‑1 (MQ=255)
              tGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAg                                                                                      >  1:1414515/1‑100 (MQ=255)
              tGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAg                                                                                      >  1:407932/1‑100 (MQ=255)
                 cGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTGTTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAg                                                                                   >  1:4545848/1‑100 (MQ=255)
                 cGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAg                                                                                   >  1:4506179/1‑100 (MQ=255)
                  gATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGc                                                                                  <  1:35468/100‑1 (MQ=255)
                  gATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGc                                                                                  >  2:3140529/1‑100 (MQ=255)
                   aTAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCt                                                                                 >  2:183222/1‑100 (MQ=255)
                     aGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTaa                                                                               <  1:1710056/100‑1 (MQ=255)
                       cggcggCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAAc                                                                             >  2:2979810/1‑100 (MQ=255)
                       cggcggCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAAc                                                                             <  1:4867286/100‑1 (MQ=255)
                        ggcggcTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACa                                                                            >  1:228693/1‑100 (MQ=255)
                          cgggTGGGAGCGTTGGTTCGTTGCCGGTATTGCGATTGGTATTAGCGTCATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAat                                                                          <  2:5341678/100‑1 (MQ=255)
                             cTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATc                                                                       >  1:4363691/1‑100 (MQ=255)
                               ggCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGc                                                                     >  1:5228491/1‑100 (MQ=255)
                                gCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTCTTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCt                                                                    >  1:3760513/1‑100 (MQ=255)
                                gCAGCGTTGGTTCTTTGCCGGTATTCCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCt                                                                    >  1:4981873/1‑100 (MQ=255)
                                gCAGCGTTGGTTCTTGGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCt                                                                    <  2:5278510/100‑1 (MQ=255)
                                   gCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAc                                                                 >  1:2149540/1‑100 (MQ=255)
                                    cGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCtgtcg                                                                >  1:840205/1‑96 (MQ=255)
                                       tGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACgcgc                                                             >  1:2474927/1‑100 (MQ=255)
                                       tGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACgcgc                                                             <  2:2341712/100‑1 (MQ=255)
                                         gTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTg                                                           >  1:2332580/1‑100 (MQ=255)
                                           tCTTTGCCGGTATTGCGATTGGTATTAGCGTGCTCCTGGCAGTTCTGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGat                                                         <  2:3822875/100‑1 (MQ=255)
                                            gtttGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCGACGCAGAAGCTAAACAATATCGCTGACGCGCGGatt                                                        >  2:3525061/2‑100 (MQ=255)
                                            cTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGatt                                                        >  1:3182422/1‑100 (MQ=255)
                                            cTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGatt                                                        <  1:2919863/100‑1 (MQ=255)
                                               tGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCGGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGattatt                                                     <  1:2115133/100‑1 (MQ=255)
                                                gCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTg                                                    >  1:1036790/1‑100 (MQ=255)
                                                 ccGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTgg                                                   <  2:628025/100‑1 (MQ=255)
                                                  cGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggc                                                  <  2:4429299/100‑1 (MQ=255)
                                                   ggTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggcg                                                 <  1:4043362/100‑1 (MQ=255)
                                                   ggTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggcg                                                 >  1:3948811/1‑100 (MQ=255)
                                                   ggTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggcg                                                 >  1:4610075/1‑100 (MQ=255)
                                                    gTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggcgg                                                <  2:2043223/100‑1 (MQ=255)
                                                    gTATGGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggcgg                                                >  2:4668147/1‑100 (MQ=255)
                                                     tATTGCGATTTGTATTAGCCTGTTCCTTGCATTGATGATGTATCGCTTGAAGGCCACGCAGAAGCTAAACAATATCGCTTACTCGCTGATTATTggcggc                                               <  2:3603576/100‑1 (MQ=255)
                                                     tATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggcggc                                               <  1:5295347/100‑1 (MQ=255)
                                                      aTTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGgcggcg                                              <  1:3875131/100‑1 (MQ=255)
                                                           gATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTg                                         >  1:3901522/1‑100 (MQ=255)
                                                           gATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTg                                         <  1:3007938/100‑1 (MQ=255)
                                                            aTTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTgg                                        <  2:5218045/100‑1 (MQ=255)
                                                              tGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGc                                      >  2:2786384/1‑100 (MQ=255)
                                                               cgatctAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCa                                     <  2:5029975/95‑1 (MQ=255)
                                                                  aTTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAAcc                                  <  2:3881507/100‑1 (MQ=255)
                                                                  aTTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAAcc                                  <  1:397121/100‑1 (MQ=255)
                                                                  aTTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAAcc                                  >  2:141495/1‑100 (MQ=255)
                                                                    tAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTg                                >  2:3448529/1‑100 (MQ=255)
                                                                    tAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTg                                <  2:1762715/100‑1 (MQ=255)
                                                                      gCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGtt                              >  1:779840/1‑100 (MQ=255)
                                                                       cgagATCCTAGCACTGATGCTGTCCCGCGCGACGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTc                             <  1:254889/97‑1 (MQ=255)
                                                                       cGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTc                             <  1:4299469/100‑1 (MQ=255)
                                                                       cGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTc                             <  1:3373134/100‑1 (MQ=255)
                                                                       cGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTc                             <  1:2625843/100‑1 (MQ=255)
                                                                       cGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTc                             >  2:4957692/1‑100 (MQ=255)
                                                                        gTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCg                            >  2:1267180/1‑100 (MQ=255)
                                                                        gTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCg                            >  1:835739/1‑100 (MQ=255)
                                                                        gTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCg                            >  1:4218399/1‑100 (MQ=255)
                                                                        gTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCg                            <  1:1249066/100‑1 (MQ=255)
                                                                        gTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCg                            <  1:2865895/100‑1 (MQ=255)
                                                                        gTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCg                            <  2:4026845/100‑1 (MQ=255)
                                                                        gTGAGCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCg                            <  2:3982100/100‑1 (MQ=255)
                                                                          gATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTCCGCGCTGATCATTGGCGGCGCGCTGGGCAACCTGTTCGAc                          >  2:2206035/1‑100 (MQ=255)
                                                                          gATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGAc                          >  2:4406504/1‑100 (MQ=255)
                                                                           aTCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGAcc                         <  2:3762713/100‑1 (MQ=255)
                                                                              cTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGcc                      >  2:1175752/1‑100 (MQ=255)
                                                                              cTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGcc                      <  2:1581819/100‑1 (MQ=255)
                                                                              cTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGcc                      <  1:4725670/100‑1 (MQ=255)
                                                                               tGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCt                     <  1:3333735/100‑1 (MQ=255)
                                                                               tGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCt                     >  1:3493260/1‑100 (MQ=255)
                                                                                  cAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCtgtg                  <  1:185040/100‑1 (MQ=255)
                                                                                  cAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCtgtg                  >  2:432508/1‑100 (MQ=255)
                                                                                   aGTTATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTgg                 <  1:5553698/100‑1 (MQ=255)
                                                                                   aGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTgg                 <  1:2813280/100‑1 (MQ=255)
                                                                                    gTGATGATGTATCGCGCGAAGGCCACGCTGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGc                <  1:5060480/100‑1 (MQ=255)
                                                                                    gTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGc                <  1:2029527/100‑1 (MQ=255)
                                                                                    gTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGc                >  2:4746890/1‑100 (MQ=255)
                                                                                       atgatgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACg             >  2:1648293/1‑100 (MQ=255)
                                                                                       atgatgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACg             >  1:5209849/1‑100 (MQ=255)
                                                                                       atgatgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACg             <  1:3219575/100‑1 (MQ=255)
                                                                                         gatgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGc           >  2:771313/1‑100 (MQ=255)
                                                                                          atgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCt          <  2:2282354/100‑1 (MQ=255)
                                                                                           tgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCtt         <  1:4382986/100‑1 (MQ=255)
                                                                                           tgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCCGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTTGCACGGCtt         <  2:5341835/100‑1 (MQ=255)
                                                                                            gTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTc        >  1:993321/1‑100 (MQ=255)
                                                                                            gTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTc        >  1:342062/1‑100 (MQ=255)
                                                                                             tATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCg       <  2:2765444/100‑1 (MQ=255)
                                                                                              aTCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGt      >  1:5496780/1‑100 (MQ=255)
                                                                                              aTCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGt      >  1:1168764/1‑100 (MQ=255)
                                                                                               tCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGtt     <  2:3202934/100‑1 (MQ=255)
                                                                                                cgcgcgAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTg    >  2:4169361/1‑100 (MQ=255)
                                                                                                cgcgcgAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTg    >  1:176360/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
GCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTC  >  NC_000913/25375‑25572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: