Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 480,566 A→C L48W (TTG→TGG)  tomB ← Hha toxicity attenuator; conjugation‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913480,5660AC99.6% 804.2 / NA 227L48W (TTG→TGG) tomBHha toxicity attenuator; conjugation‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (114/112);  minor base T (1/0);  total (115/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

CAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAA  >  NC_000913/480469‑480662
                                                                                                 |                                                                                                
cAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCAc                                                                                                >  2:2878780/1‑100 (MQ=255)
cAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCAc                                                                                                >  2:790613/1‑100 (MQ=255)
cAGATATTCGTCGATCTACTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCAc                                                                                                >  1:2430400/1‑100 (MQ=255)
 aGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACt                                                                                               >  2:3334328/1‑100 (MQ=255)
  gATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTg                                                                                              <  2:2408281/100‑1 (MQ=255)
  gATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTg                                                                                              <  1:4219778/100‑1 (MQ=255)
    tatTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGa                                                                                            >  2:4287317/1‑100 (MQ=255)
    tatTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGa                                                                                            <  1:3690351/100‑1 (MQ=255)
     atTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAg                                                                                           <  2:1431178/100‑1 (MQ=255)
     atTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAg                                                                                           <  1:2829195/100‑1 (MQ=255)
     atTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAg                                                                                           <  1:3769956/100‑1 (MQ=255)
     atTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAg                                                                                           <  1:1306168/100‑1 (MQ=255)
       tcgtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGt                                                                                         <  1:1171105/100‑1 (MQ=255)
        cgtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGtt                                                                                        >  2:3212412/1‑100 (MQ=255)
        cgtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGtt                                                                                        >  1:750578/1‑100 (MQ=255)
            gATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATc                                                                                    >  2:5281439/1‑100 (MQ=255)
            gATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATc                                                                                    <  1:2475879/100‑1 (MQ=255)
              tCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGc                                                                                  >  2:577410/1‑100 (MQ=255)
               cTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTACTTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGcc                                                                                 >  1:4741025/1‑100 (MQ=255)
                tGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCg                                                                                >  2:2296977/1‑100 (MQ=255)
                 gCTCAATGAGCTTCTTGTCTTCATTATACTTAACTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGa                                                                               >  2:4602153/1‑100 (MQ=255)
                    cAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGt                                                                            >  2:1818940/1‑100 (MQ=255)
                    cAATAAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGt                                                                            <  1:4990678/100‑1 (MQ=255)
                       tGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTgg                                                                         <  1:672478/100‑1 (MQ=255)
                       tGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTgg                                                                         >  1:141454/1‑100 (MQ=255)
                        gAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTggg                                                                        <  1:1616036/100‑1 (MQ=255)
                        gAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTggg                                                                        <  1:1685000/100‑1 (MQ=255)
                        gAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTgag                                                                        >  2:4781731/1‑98 (MQ=255)
                         aGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGt                                                                       <  1:246024/100‑1 (MQ=255)
                          gCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTc                                                                      >  2:3443553/1‑100 (MQ=255)
                          gCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTc                                                                      >  2:1046258/1‑100 (MQ=255)
                           cTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCg                                                                     >  2:205642/1‑100 (MQ=255)
                           cTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCg                                                                     >  2:664668/1‑100 (MQ=255)
                             tATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGtt                                                                   <  2:743834/100‑1 (MQ=255)
                              aTTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGATGGTCGGAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTAGttt                                                                  <  2:4659982/100‑1 (MQ=255)
                              aTTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGttt                                                                  <  2:3590340/100‑1 (MQ=255)
                               ttGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTa                                                                 <  2:2028863/100‑1 (MQ=255)
                                tGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAc                                                                <  1:1223932/100‑1 (MQ=255)
                                tGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAc                                                                >  2:518936/1‑100 (MQ=255)
                                 gTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAcc                                                               >  2:5154071/1‑100 (MQ=255)
                                 gTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAcc                                                               >  1:2661734/1‑100 (MQ=255)
                                  tCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAccc                                                              <  1:4481982/100‑1 (MQ=255)
                                  tCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAccc                                                              <  1:569823/100‑1 (MQ=255)
                                   cTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCa                                                             >  1:260153/1‑100 (MQ=255)
                                      cATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGcc                                                          >  2:3015435/1‑100 (MQ=255)
                                       aTTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCa                                                         <  1:1424974/100‑1 (MQ=255)
                                       aTTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCa                                                         >  2:1149297/1‑100 (MQ=255)
                                       aTTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCa                                                         >  1:4335718/1‑100 (MQ=255)
                                       aTTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCa                                                         <  2:1632501/100‑1 (MQ=255)
                                       aTTATACTTAATTTTGTAATTAACTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCa                                                         >  1:3625246/1‑100 (MQ=255)
                                           taCTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGAt                                                     <  2:3722925/100‑1 (MQ=255)
                                            aCTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGAtt                                                    <  1:2027205/100‑1 (MQ=255)
                                            aCTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGAtt                                                    <  1:3726859/100‑1 (MQ=255)
                                             cTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTg                                                   >  2:3885134/1‑100 (MQ=255)
                                              ttAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGc                                                  <  2:5375199/100‑1 (MQ=255)
                                               tAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCt                                                 >  2:869957/1‑100 (MQ=255)
                                                aaTTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAACCATAATTGCtt                                                >  1:5041522/1‑100 (MQ=255)
                                                 aTTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCttt                                               <  1:949498/100‑1 (MQ=255)
                                                  ttttGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttc                                              <  1:5277864/100‑1 (MQ=255)
                                                   tttGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttct                                             >  2:614747/1‑100 (MQ=255)
                                                   tttGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttct                                             <  2:1692312/100‑1 (MQ=255)
                                                   tttGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttct                                             >  1:1816560/1‑100 (MQ=255)
                                                      gTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGGGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCa                                          >  1:4101761/1‑100 (MQ=255)
                                                      gTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCa                                          >  2:23609/1‑100 (MQ=255)
                                                        aaTTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAg                                        >  1:5542672/1‑100 (MQ=255)
                                                         aTTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg                                       >  2:1480563/1‑100 (MQ=255)
                                                         aTTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg                                       >  2:1809437/1‑100 (MQ=255)
                                                         aTTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg                                       <  2:436110/100‑1 (MQ=255)
                                                         aTTAAGCGCGAAGGTCTGAATATGCTCAATCAGCTCATTCCCCTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg                                       <  1:4587663/100‑1 (MQ=255)
                                                          ttAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGt                                      >  1:1503172/1‑100 (MQ=255)
                                                          ttAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGACGAGGTTGGGTCGTTTACCCAGCCATCATTGCTTTCATCAAGGt                                      >  1:5118831/1‑100 (MQ=255)
                                                            aaGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGttt                                    <  1:3587147/100‑1 (MQ=255)
                                                             aGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTg                                   <  1:3062016/100‑1 (MQ=255)
                                                                gCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCaa                                >  2:5329488/1‑100 (MQ=255)
                                                                gCGAAGGTCGCAATATGCTCAATAAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCCGCGCTGCTTGCTTTCTTCAAGGTTTGCaa                                >  2:2435728/1‑100 (MQ=255)
                                                                 ngAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCCAg                               >  1:4431909/2‑100 (MQ=255)
                                                                     ggTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAGGGCAg                           >  1:3317614/1‑100 (MQ=255)
                                                                     ggTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAg                           >  1:4726486/1‑100 (MQ=255)
                                                                     ggTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAg                           <  1:2162932/100‑1 (MQ=255)
                                                                     ggTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAg                           >  1:1921453/1‑100 (MQ=255)
                                                                        cGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCa                        >  2:2637977/1‑100 (MQ=255)
                                                                           aaTATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGa                     <  2:4004009/100‑1 (MQ=255)
                                                                           aaTATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGa                     <  1:1041399/100‑1 (MQ=255)
                                                                           aaTATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGa                     <  1:2320563/100‑1 (MQ=255)
                                                                            atatGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGAt                    >  2:2138257/1‑100 (MQ=255)
                                                                                gCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAg                >  1:3093926/1‑100 (MQ=255)
                                                                                gCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGCTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAg                <  1:5415629/100‑1 (MQ=255)
                                                                                 cTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAgg               >  2:1187840/1‑100 (MQ=255)
                                                                                   cAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAGGGTTTGCAAGGCAGTCATGATACAGGGt             >  2:3808111/1‑100 (MQ=255)
                                                                                   cAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGt             >  2:3991696/1‑100 (MQ=255)
                                                                                     aTCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGttt           <  1:3107749/100‑1 (MQ=255)
                                                                                      tCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTc          <  1:3040987/100‑1 (MQ=255)
                                                                                       cAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca         <  2:1270617/100‑1 (MQ=255)
                                                                                       cAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca         <  2:4067380/100‑1 (MQ=255)
                                                                                         gTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcaca       >  2:898121/1‑100 (MQ=255)
                                                                                         gTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcaca       >  2:3292383/1‑100 (MQ=255)
                                                                                         gTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcaca       <  1:1060298/100‑1 (MQ=255)
                                                                                          ttcattccACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACag      <  1:4714568/100‑1 (MQ=255)
                                                                                            cattccACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACagag    <  2:3374717/100‑1 (MQ=255)
                                                                                              ttccACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGaa  >  2:3039046/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAA  >  NC_000913/480469‑480662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑