Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,972,452 A→C Q398P (CAG→CCG)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,972,4520AC99.6% 852.7 / ‑5.4 240Q398P (CAG→CCG) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (119/119);  total (121/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 240 total aligned reads displayed.

CAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGT  >  NC_000913/3972355‑3972548
                                                                                                 |                                                                                                
 aGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGtt                                                                                               <  2:108914/100‑1 (MQ=255)
  gTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTc                                                                                              >  2:1869925/1‑100 (MQ=255)
   ttGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCa                                                                                             <  2:3619506/100‑1 (MQ=255)
   ttGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCa                                                                                             <  2:1275823/100‑1 (MQ=255)
   ttGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCa                                                                                             >  2:2849345/1‑100 (MQ=255)
    tGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCaa                                                                                            >  1:4892764/1‑100 (MQ=255)
    cgCCGAAAAACCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCaa                                                                                            <  2:4743672/99‑1 (MQ=255)
     gCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCaaa                                                                                           <  2:4711323/100‑1 (MQ=255)
      ccGGAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAg                                                                                          <  2:1720503/100‑1 (MQ=255)
      ccGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAg                                                                                          <  2:5171117/100‑1 (MQ=255)
       cGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGt                                                                                         <  1:491252/100‑1 (MQ=255)
        gAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGtt                                                                                        >  2:214817/1‑100 (MQ=255)
        gAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGtt                                                                                        >  1:3400688/1‑100 (MQ=255)
        gAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGtt                                                                                        >  2:2597361/1‑100 (MQ=255)
        gAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGtt                                                                                        >  2:3159585/1‑100 (MQ=255)
        gAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGtt                                                                                        >  2:704134/1‑100 (MQ=255)
        gAAAAAACTGACCGGGCTTGGTACGCTGGCGCAGCTTGACGAGGCGCAGGCAACGGCAGACGTGCTGGTGATGCGGGTCGATGATAGTCCGTTCAAAGtg                                                                                        >  2:1122269/1‑99 (MQ=255)
          aaaaaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCTATCATAGTCCGTTCAAAGTTAt                                                                                      <  2:1152391/100‑1 (MQ=255)
          aaaaaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTAt                                                                                      >  1:4010604/1‑100 (MQ=255)
           aaaaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATc                                                                                     <  2:2100544/100‑1 (MQ=255)
           aaaaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATc                                                                                     <  2:2607117/100‑1 (MQ=255)
            aaaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCa                                                                                    >  2:574960/1‑100 (MQ=255)
             aaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCaa                                                                                   <  2:2926169/100‑1 (MQ=255)
             aaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCaa                                                                                   <  1:5426983/100‑1 (MQ=255)
               cTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATg                                                                                 >  1:3620282/1‑100 (MQ=255)
                tGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATgg                                                                                <  2:2703222/100‑1 (MQ=255)
                   ccGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGa                                                                             >  2:2387083/1‑100 (MQ=255)
                   ccGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGa                                                                             <  1:111324/100‑1 (MQ=255)
                   ccGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGa                                                                             <  1:860055/100‑1 (MQ=255)
                     gggCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACa                                                                           >  1:1012038/1‑100 (MQ=255)
                     gggCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACa                                                                           >  1:1298158/1‑100 (MQ=255)
                       gCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAAt                                                                         >  2:2007085/1‑100 (MQ=255)
                        cTTTGTACTCTGGTGCAGCTTGACGATGCGCTGGCAACGGCAGACGTGCGGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATg                                                                        <  2:4313741/100‑1 (MQ=255)
                         tttGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGt                                                                       >  1:4380551/1‑100 (MQ=255)
                         tttGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGt                                                                       <  1:4042284/100‑1 (MQ=255)
                         tttGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGt                                                                       <  1:5102385/100‑1 (MQ=255)
                         tttGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGt                                                                       >  1:3671908/1‑100 (MQ=255)
                           tGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTcc                                                                     <  1:2622944/100‑1 (MQ=255)
                           tGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTcc                                                                     <  1:4767608/100‑1 (MQ=255)
                            gTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCGGTTCAAAGTTATCAATGGCGACACTGTCCa                                                                    >  2:1950245/1‑100 (MQ=255)
                             tACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCAt                                                                   <  2:4373861/100‑1 (MQ=255)
                              aCTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATc                                                                  <  1:3643644/100‑1 (MQ=255)
                                  tGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATcagca                                                              <  2:79099/100‑1 (MQ=255)
                                   ggCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATcagcag                                                             <  1:5248710/100‑1 (MQ=255)
                                    gcgcAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGt                                                            <  2:4520469/100‑1 (MQ=255)
                                     cgcAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTa                                                           >  1:5499047/1‑100 (MQ=255)
                                      gcAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTAt                                                          <  1:2249784/100‑1 (MQ=255)
                                      gcAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTAt                                                          <  2:3226841/100‑1 (MQ=255)
                                       cAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATg                                                         >  1:2359754/1‑100 (MQ=255)
                                       cAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATg                                                         <  2:4880450/100‑1 (MQ=255)
                                       cAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATCTCCATCAGCAGTATg                                                         <  1:1483672/100‑1 (MQ=255)
                                        aGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgt                                                        >  2:1133040/1‑100 (MQ=255)
                                        aGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgt                                                        <  1:1672190/100‑1 (MQ=255)
                                        aGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgt                                                        <  2:4058331/100‑1 (MQ=255)
                                           ttGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtcgt                                                     >  2:3086565/1‑100 (MQ=255)
                                            tGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtcgtc                                                    >  2:1788701/1‑100 (MQ=255)
                                            tGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtcgtc                                                    <  1:2453336/100‑1 (MQ=255)
                                            tGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtcgtc                                                    <  1:413687/100‑1 (MQ=255)
                                            tGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtcgtc                                                    >  1:634569/1‑100 (MQ=255)
                                               cGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGAt                                                 <  1:3115480/100‑1 (MQ=255)
                                               cGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGAt                                                 <  1:4800296/100‑1 (MQ=255)
                                                gAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATg                                                <  2:4239041/100‑1 (MQ=255)
                                                gAGGCGCGGGCAGAGGCAGACGTGCTGGTGATGCTGGTCGATCAGAGTCCGGTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATg                                                <  2:514370/100‑1 (MQ=255)
                                                  ggCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGcc                                              <  2:4451605/100‑1 (MQ=255)
                                                      cTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAg                                          >  2:5039167/1‑100 (MQ=255)
                                                       tGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAgg                                         <  1:2906977/100‑1 (MQ=255)
                                                          cAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGCTGCCAAAGGAgg                                      >  1:4595834/1‑99 (MQ=255)
                                                          cAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGt                                      >  1:4548718/1‑100 (MQ=255)
                                                           aaCGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTc                                     <  2:4000589/100‑1 (MQ=255)
                                                              ggCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTgg                                  >  2:2557379/1‑100 (MQ=255)
                                                                cAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGgcg                                <  2:848107/100‑1 (MQ=255)
                                                                cAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGgcg                                >  1:3917970/1‑100 (MQ=255)
                                                                  gACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCt                              >  1:919331/1‑100 (MQ=255)
                                                                     gTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCtgat                           <  1:3212358/100‑1 (MQ=255)
                                                                      tGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCtgatg                          >  1:3486255/1‑100 (MQ=255)
                                                                       gCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCtgatga                         <  2:4806989/100‑1 (MQ=255)
                                                                        cTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATgag                        >  1:383131/1‑100 (MQ=255)
                                                                        cTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATgag                        >  2:1513665/1‑100 (MQ=255)
                                                                        cTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCAATCAGCAGTATGTCTTCGATGCCAAAGGAGTCTGGCGCTGATgag                        >  2:142564/1‑100 (MQ=255)
                                                                           gTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGaaa                     <  2:1374813/100‑1 (MQ=255)
                                                                           gTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGaaa                     <  2:4665965/100‑1 (MQ=255)
                                                                              aTGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAAt                  >  1:2194932/1‑100 (MQ=255)
                                                                              aTGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAAt                  >  2:2459528/1‑100 (MQ=255)
                                                                               tGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCGAAAGGAGGCTGGAGCTGGTGAGAAAACtt                 >  2:2050208/1‑100 (MQ=255)
                                                                               tGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAAtt                 >  1:1741468/1‑100 (MQ=255)
                                                                                gCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTc                <  1:2069138/100‑1 (MQ=255)
                                                                                gCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTc                <  1:5373014/100‑1 (MQ=255)
                                                                                gCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTc                >  2:4185823/1‑100 (MQ=255)
                                                                                 cTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCt               <  1:2466807/100‑1 (MQ=255)
                                                                                  tGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTg              <  1:1476728/100‑1 (MQ=255)
                                                                                   ggTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGa             >  2:333213/1‑100 (MQ=255)
                                                                                   ggTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGa             <  1:722792/100‑1 (MQ=255)
                                                                                    ggccatcatcGTCCGTTCAAAGGTATCAATGGCGACAATGTCCAGCAGTAGTATGTCGACGATGCCAAAGGAGTCTGGAGCTGATGCGAAAAATTCTGAt            <  2:658503/96‑1 (MQ=255)
                                                                                      cGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATaa          >  1:5220376/1‑100 (MQ=255)
                                                                                       gATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAAc         >  2:4672756/1‑100 (MQ=255)
                                                                                       gATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAAc         >  1:2535561/1‑100 (MQ=255)
                                                                                        aTCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGCTAACa        <  2:37462/100‑1 (MQ=255)
                                                                                         tCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAg       <  1:3971553/100‑1 (MQ=255)
                                                                                          cATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAgg      >  1:4225920/1‑100 (MQ=255)
                                                                                              gTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAggtggt  >  2:3895661/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGT  >  NC_000913/3972355‑3972548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑