Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,046,941 A→C intergenic (‑339/‑25) yihG ← / → polA inner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,046,9410AC98.7% 802.1 / ‑4.3 224intergenic (‑339/‑25)yihG/polAinner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (89/132);  total (91/133)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

ACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCATATCACGC  >  NC_000913/4046845‑4047036
                                                                                                |                                                                                               
aCGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCatcatc                                                                                              <  2:563803/100‑1 (MQ=255)
aCGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCatcatc                                                                                              <  2:5279595/100‑1 (MQ=255)
 cGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCt                                                                                             <  1:693937/100‑1 (MQ=255)
 cGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCt                                                                                             >  1:5332003/1‑100 (MQ=255)
 cGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCATGTCTGTGGCATCCTTTACCCATCATCt                                                                                             >  2:3223876/1‑100 (MQ=255)
  gATTTGCGAGCGATCCAGCAGATCTACAAAGGATTTTAACGAAAAGCTGTGAAGAACCCAGGATTTGATCCTGGCCGTGGCATCCTTGACCCATCATCTg                                                                                            >  2:1074912/1‑100 (MQ=255)
  gATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTg                                                                                            <  2:820832/100‑1 (MQ=255)
   aTTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGa                                                                                           <  2:4759454/100‑1 (MQ=255)
   aTTTGCGAGCGATCCAGAAGATCTACAAAAGATTCTCACGAAAAGCGGTGAAACACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGa                                                                                           >  2:5074332/1‑100 (MQ=255)
    tttGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGAt                                                                                          <  2:32672/100‑1 (MQ=255)
     ttGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATa                                                                                         <  1:1063530/100‑1 (MQ=255)
     ttGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATa                                                                                         <  2:2750261/100‑1 (MQ=255)
         gagcgaTCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACa                                                                                     >  1:2946152/1‑100 (MQ=255)
          agcggTCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAg                                                                                    >  2:4648175/1‑100 (MQ=255)
            cgaTCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGc                                                                                  >  2:3832749/1‑100 (MQ=255)
            cgaTCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGc                                                                                  >  2:1518099/1‑100 (MQ=255)
            cgaTCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGc                                                                                  <  1:4266757/100‑1 (MQ=255)
            cgaTCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGc                                                                                  <  1:164098/100‑1 (MQ=255)
             gaTCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCa                                                                                 <  1:975701/100‑1 (MQ=255)
             gaTCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTAACCATCATCTGATAAACAGGCa                                                                                 <  2:3121842/100‑1 (MQ=255)
              aTCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCAc                                                                                >  1:4618310/1‑100 (MQ=255)
              aTCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCAc                                                                                >  2:1793812/1‑100 (MQ=255)
                ccAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACgg                                                                              <  1:2535254/100‑1 (MQ=255)
                 cAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGa                                                                             >  1:1356518/1‑100 (MQ=255)
                  agaagaTCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGAc                                                                            <  2:5085806/100‑1 (MQ=255)
                  agaagaCCTACAAACGATTTTCACGAAAAGCGGTGAAAATCTCATGCTCTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGAc                                                                            <  2:3085082/100‑1 (MQ=255)
                   gaagaTCTACAACAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTAGCATCCTTTACCCATCATCTGATAAACAGGCACGGACa                                                                           >  2:5293935/1‑100 (MQ=255)
                   gaagaTCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATCTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACa                                                                           <  1:2661104/100‑1 (MQ=255)
                    aagaTCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACAt                                                                          <  1:5046554/100‑1 (MQ=255)
                      gaTCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTa                                                                        <  1:5145120/100‑1 (MQ=255)
                        tCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATg                                                                      <  1:1510907/100‑1 (MQ=255)
                         cTACAAAAGATTTTCACGAAAAGCGGTGCAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATgg                                                                     <  1:4063945/100‑1 (MQ=255)
                         cTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATgg                                                                     >  2:2886377/1‑100 (MQ=255)
                         cTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATgg                                                                     <  2:4868783/100‑1 (MQ=255)
                          taaaaaaGAGTTTCACGAAAAGCGGGGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGt                                                                    <  2:4363613/97‑1 (MQ=255)
                          tACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGt                                                                    >  1:2893416/1‑100 (MQ=255)
                          tACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGt                                                                    <  2:2850553/100‑1 (MQ=255)
                          tACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGt                                                                    >  2:2823058/1‑100 (MQ=255)
                           aCAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGtt                                                                   >  2:4033464/1‑100 (MQ=255)
                            cAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTc                                                                  <  1:469635/100‑1 (MQ=255)
                            cAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTc                                                                  <  1:4375687/100‑1 (MQ=255)
                            cAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTc                                                                  <  1:932935/100‑1 (MQ=255)
                              aaaGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAg                                                                >  1:2709274/1‑100 (MQ=255)
                               aaGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGa                                                               <  1:5352845/100‑1 (MQ=255)
                                 taTTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATc                                                             <  1:3287077/99‑1 (MQ=255)
                                 gATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATc                                                             <  2:5237031/100‑1 (MQ=255)
                                  aTTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATcc                                                            <  1:3120742/100‑1 (MQ=255)
                                     ttCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATccccc                                                         <  1:1152627/100‑1 (MQ=255)
                                     ttCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATccccc                                                         <  2:2918438/100‑1 (MQ=255)
                                      tCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCa                                                        >  2:3402252/1‑100 (MQ=255)
                                      tCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCa                                                        <  2:3706054/100‑1 (MQ=255)
                                       cACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCaa                                                       <  1:1291400/100‑1 (MQ=255)
                                       cACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTGCAGATcccccca                                                       >  2:4145831/1‑98 (MQ=255)
                                        aCGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCaaa                                                      >  1:2361323/1‑100 (MQ=255)
                                            aaaGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATcc                                                  >  2:426389/1‑100 (MQ=255)
                                            aaaGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATcc                                                  <  2:3445571/100‑1 (MQ=255)
                                            aaaGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATcc                                                  <  2:2831238/100‑1 (MQ=255)
                                              aGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCAc                                                <  2:4772524/100‑1 (MQ=255)
                                              aGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCAc                                                >  2:296059/1‑100 (MQ=255)
                                               gCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACt                                               <  1:777313/100‑1 (MQ=255)
                                                  gTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTAt                                            >  2:4948371/1‑100 (MQ=255)
                                                    gAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATcc                                          >  2:3473988/1‑100 (MQ=255)
                                                         aCTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACATGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTa                                     <  2:606170/100‑1 (MQ=255)
                                                         aCTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTa                                     <  2:1732920/100‑1 (MQ=255)
                                                         aCTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTa                                     <  1:5163683/100‑1 (MQ=255)
                                                         aCTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTa                                     <  2:3563376/100‑1 (MQ=255)
                                                             aTGTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATg                                 <  1:2822426/100‑1 (MQ=255)
                                                               gTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGt                               >  2:2405459/1‑100 (MQ=255)
                                                               gTTTTCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGt                               <  2:2132034/100‑1 (MQ=255)
                                                                   tCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCat                           <  1:5264743/100‑1 (MQ=255)
                                                                   tCATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCat                           >  2:1998119/1‑100 (MQ=255)
                                                                    cATCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCatc                          >  1:3750529/1‑100 (MQ=255)
                                                                     aTCCTGTCTGTGGCATCCTTTACCCAGCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCatct                         <  2:2966677/100‑1 (MQ=255)
                                                                      tCCTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCatctn                        <  1:1198846/100‑2 (MQ=255)
                                                                       ccTGTCTGTGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAACATCCACTTATCCTTGTACATGGTTCatctta                       >  2:4306609/1‑100 (MQ=255)
                                                                        ctgtctttGGCATCCTTTACCCATCATCTGCTAAACAGGCACGGATATTATGGTTCAGAACCCCCAAAATCCACTTATCCTTGTAGATGGTTCttgttat                      <  2:5168847/100‑1 (MQ=255)
                                                                        ctgtctgtGGCATCCTTTACCCATCCTCTGATAAACAGGCACGGACATTATGGTTCAGGTCCCCCAAAATCCACTTAGCCTTGTAGATGGTTCatcttat                      <  2:2210821/100‑1 (MQ=255)
                                                                         tgtctgtGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCatcttatc                     >  1:3378470/1‑100 (MQ=255)
                                                                         tgtctgtGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCatcttatc                     >  1:1141512/1‑100 (MQ=255)
                                                                            ctgtGGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCttt                  >  1:2229272/1‑100 (MQ=255)
                                                                             tgtgGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTa                 >  1:1445114/1‑100 (MQ=255)
                                                                             tgtgGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTa                 >  1:1809572/1‑100 (MQ=255)
                                                                               tgGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATc               <  2:2551976/100‑1 (MQ=255)
                                                                               tgGCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATc               <  1:494247/100‑1 (MQ=255)
                                                                                ggCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATcg              >  2:2272429/1‑100 (MQ=255)
                                                                                ggCATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATcg              <  1:890560/100‑1 (MQ=255)
                                                                                gacATCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATcg              >  1:1329441/3‑100 (MQ=255)
                                                                                    tCCTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCa          <  1:2464711/100‑1 (MQ=255)
                                                                                     ccTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCat         >  2:2379194/1‑100 (MQ=255)
                                                                                     ccTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCat         <  1:2838139/100‑1 (MQ=255)
                                                                                     ccTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCat         <  1:4701007/100‑1 (MQ=255)
                                                                                     ccTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCat         <  2:1203597/100‑1 (MQ=255)
                                                                                     ccTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCat         >  2:1621712/1‑100 (MQ=255)
                                                                                      cTTTACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCata        >  1:5435057/1‑100 (MQ=255)
                                                                                       tttACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCatat       <  1:3777384/100‑1 (MQ=255)
                                                                                       tttACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCatat       <  1:1394783/100‑1 (MQ=255)
                                                                                         tACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCATATCa     >  1:4407033/1‑100 (MQ=255)
                                                                                         tACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCATATCa     <  1:3914834/100‑1 (MQ=255)
                                                                                         tACCCATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCATATCa     >  1:2064069/1‑100 (MQ=255)
                                                                                           cccATCATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCATATCAcg   >  1:420903/1‑100 (MQ=255)
                                                                                                |                                                                                               
ACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCATATCACGC  >  NC_000913/4046845‑4047036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑