Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,190,100 T→G I105M (ATT→ATG yjaZ → stationary phase growth adaptation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,190,1000TG99.4% 640.6 / NA 179I105M (ATT→ATGyjaZstationary phase growth adaptation protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (92/85);  minor base A (1/0);  total (94/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

CGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAA  >  NC_000913/4190003‑4190195
                                                                                                 |                                                                                               
 gcCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCAt                                                                                              <  2:2806605/100‑1 (MQ=255)
 gcCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCAt                                                                                              <  1:2410172/100‑1 (MQ=255)
    tAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGcc                                                                                           >  1:1047618/1‑100 (MQ=255)
    tAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGcc                                                                                           >  2:3435298/1‑100 (MQ=255)
    tAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGcc                                                                                           >  1:4751367/1‑100 (MQ=255)
    tAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGcc                                                                                           <  1:4031236/100‑1 (MQ=255)
    tAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGcc                                                                                           >  1:3802927/1‑100 (MQ=255)
    tAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGcc                                                                                           <  1:3261663/100‑1 (MQ=255)
     aaaGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCg                                                                                          >  1:3534681/1‑100 (MQ=255)
      aaGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCgg                                                                                         >  2:1676473/1‑100 (MQ=255)
       aGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCggg                                                                                        >  2:1284724/1‑100 (MQ=255)
       aGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCggg                                                                                        <  1:3542096/100‑1 (MQ=255)
       aGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCggg                                                                                        <  1:2946988/100‑1 (MQ=255)
         aaTCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTa                                                                                      <  1:1567645/100‑1 (MQ=255)
         aaTCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTa                                                                                      <  1:1694157/100‑1 (MQ=255)
          aTCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTAt                                                                                     >  2:4587033/1‑100 (MQ=255)
            ctcGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATgg                                                                                   >  2:3584909/1‑100 (MQ=255)
            ctcGTCCCTTCTGCCAGAACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAACGCAAAGTTATGAATGCCGGGTATgg                                                                                   <  2:3898774/100‑1 (MQ=255)
             tcGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGt                                                                                  <  2:3158901/100‑1 (MQ=255)
               gTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAg                                                                                <  1:5524739/100‑1 (MQ=255)
                tCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGc                                                                               >  2:5444933/1‑100 (MQ=255)
                     tCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGc                                                                          <  1:1168649/100‑1 (MQ=255)
                     tCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGc                                                                          <  2:3436796/100‑1 (MQ=255)
                       tGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCg                                                                        >  1:2733948/1‑100 (MQ=255)
                        gCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGt                                                                       >  2:1843767/1‑100 (MQ=255)
                        gCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGt                                                                       >  2:4723297/1‑100 (MQ=255)
                        gCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGt                                                                       >  1:2664302/1‑100 (MQ=255)
                             cACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTg                                                                  <  1:3134240/100‑1 (MQ=255)
                               ccGTGAGAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGACAGAAAGCAACGTTATACAAGCCGGGTATGGTAGCTATGCCGTGATTGAg                                                                >  2:5182598/1‑100 (MQ=255)
                                cGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAgg                                                               <  2:4380256/100‑1 (MQ=255)
                                cGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAgg                                                               <  1:1886003/100‑1 (MQ=255)
                                cGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAgg                                                               <  2:4035266/100‑1 (MQ=255)
                                cGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAgg                                                               <  2:3768028/100‑1 (MQ=255)
                                cGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAgg                                                               <  1:5241248/100‑1 (MQ=255)
                                 gTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGt                                                              >  2:4848981/1‑100 (MQ=255)
                                  ttATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTg                                                             >  2:2242660/1‑100 (MQ=255)
                                  ttATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTg                                                             <  1:717510/100‑1 (MQ=255)
                                  ttATAACTTAGCGCGCACACACTTAAAAAGAAATATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTg                                                             <  2:5500809/100‑1 (MQ=255)
                                   tataACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTgg                                                            >  2:141631/1‑100 (MQ=255)
                                    ataACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGa                                                           >  2:2585010/1‑100 (MQ=255)
                                     taACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGAc                                                          >  2:2190216/1‑100 (MQ=255)
                                     taACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGAc                                                          <  2:2386712/100‑1 (MQ=255)
                                     taACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGAc                                                          >  2:3934184/1‑100 (MQ=255)
                                      aaCTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACt                                                         <  1:3439675/100‑1 (MQ=255)
                                       aCTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACtt                                                        >  1:4697886/1‑100 (MQ=255)
                                       aCTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACtt                                                        <  2:4441649/100‑1 (MQ=255)
                                        cTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTa                                                       <  1:1005648/100‑1 (MQ=255)
                                           aGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGAc                                                    <  1:496132/100‑1 (MQ=255)
                                               cgcACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACggag                                                >  1:5147699/1‑100 (MQ=255)
                                                gcACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACggagg                                               <  1:2910074/100‑1 (MQ=255)
                                                  acacacTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGgaggag                                             >  2:3303975/1‑100 (MQ=255)
                                                  acacacTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGgaggag                                             >  1:3172601/1‑100 (MQ=255)
                                                     cacTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATa                                          <  2:1561167/100‑1 (MQ=255)
                                                     cacTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATACCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATa                                          >  1:3639892/1‑100 (MQ=255)
                                                       cTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCTTGCCGGGAATGGGAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAg                                        >  2:755726/1‑100 (MQ=255)
                                                         tAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGc                                      >  2:3725630/1‑100 (MQ=255)
                                                          aaaaaGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCa                                     >  2:2949527/1‑100 (MQ=255)
                                                              aGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCAtttt                                 <  1:2042577/100‑1 (MQ=255)
                                                                  ccaTTTTGGCGCTCCAAGACAGCAACATTATGCATGGCGGGTATGGTAGCTATGCCGTGCTTGAGGCGGTCTTAGACGGAGGAGATAAGGCATTTTACtt                             <  2:5262835/98‑1 (MQ=255)
                                                                  cTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACtt                             >  2:133517/1‑100 (MQ=255)
                                                                  cTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACtt                             <  2:1761974/100‑1 (MQ=255)
                                                                   tATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACttt                            >  2:1950285/1‑100 (MQ=255)
                                                                   tATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACttt                            >  1:5074897/1‑100 (MQ=255)
                                                                    aTTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTttg                           <  1:3745068/100‑1 (MQ=255)
                                                                     ttttGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTttgn                          <  2:204837/100‑2 (MQ=255)
                                                                      tttGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTttgtt                         >  2:5001064/1‑100 (MQ=255)
                                                                       ttGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTttgttg                        <  2:2764121/100‑1 (MQ=255)
                                                                       ttGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTttgttg                        <  2:3784072/100‑1 (MQ=255)
                                                                        tGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGc                       >  1:838763/1‑100 (MQ=255)
                                                                        tGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGc                       <  1:3209866/100‑1 (MQ=255)
                                                                        tGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGc                       >  1:3953070/1‑100 (MQ=255)
                                                                           cgcTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGtt                    <  1:2531350/100‑1 (MQ=255)
                                                                           cgcTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGtt                    >  1:2672976/1‑100 (MQ=255)
                                                                           cgcTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGtt                    >  1:3886339/1‑100 (MQ=255)
                                                                             cTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCa                  >  2:3127405/1‑100 (MQ=255)
                                                                             cTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCa                  <  2:334334/100‑1 (MQ=255)
                                                                             cTGCCAGAAAGCAACGTTACGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCa                  <  1:4663711/100‑1 (MQ=255)
                                                                              tGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAg                 <  1:130469/100‑1 (MQ=255)
                                                                              tGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAg                 <  1:2005440/100‑1 (MQ=255)
                                                                               gCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAgg                <  1:4379199/100‑1 (MQ=255)
                                                                               gCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAgg                >  1:1467218/1‑100 (MQ=255)
                                                                                ccAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAggg               >  2:1543153/1‑100 (MQ=255)
                                                                                 cAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGc              <  1:1797650/100‑1 (MQ=255)
                                                                                 cAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGc              >  1:4821941/1‑100 (MQ=255)
                                                                                 cAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGc              >  1:1732320/1‑100 (MQ=255)
                                                                                 cAGAAAGCAACGTTATCCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGc              >  1:4877296/1‑100 (MQ=255)
                                                                                     aaGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTc          >  2:5061792/1‑100 (MQ=255)
                                                                                     aaGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTc          >  2:5389218/1‑100 (MQ=255)
                                                                                      aGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCa         >  2:1999078/1‑100 (MQ=255)
                                                                                       gCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAg        >  2:420411/1‑100 (MQ=255)
                                                                                       gCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAg        >  2:1031928/1‑100 (MQ=255)
                                                                                       gCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAg        <  1:3334908/100‑1 (MQ=255)
                                                                                       gCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAg        >  2:593122/1‑100 (MQ=255)
                                                                                       gCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAg        >  1:2219251/1‑100 (MQ=255)
                                                                                        cAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAgg       >  2:4765029/1‑100 (MQ=255)
                                                                                         aaCGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAggg      <  2:3073260/100‑1 (MQ=255)
                                                                                          aCGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGGGGTGGACTTAGTCGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGga     <  1:4183455/100‑1 (MQ=255)
                                                                                            gTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGgaaa   >  1:4535624/1‑100 (MQ=255)
                                                                                             ttATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGgaaaa  <  2:3850797/100‑1 (MQ=255)
                                                                                             ttATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGgaaaa  <  2:933944/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAA  >  NC_000913/4190003‑4190195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: