Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,215,122 T→G G281G (GGT→GGG)  metA → homoserine O‑transsuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,215,1220TG98.7% 826.7 / ‑3.3 236G281G (GGT→GGG) metAhomoserine O‑transsuccinylase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (113/118);  total (115/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 236 total aligned reads displayed.

CGATGTGGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGT  >  NC_000913/4215026‑4215217
                                                                                                |                                                                                               
cgATGTGGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGTACAATGTTCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAAt                                                                                              <  1:1053523/100‑1 (MQ=255)
 gATGTGGAAGCCGGACTAGACCCGGATGTACCGTATAACTAGTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAAtt                                                                                             >  1:5554670/1‑100 (MQ=255)
   tgtgGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGTATTTa                                                                                           >  1:2855109/1‑100 (MQ=255)
   tgtgGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTa                                                                                           >  2:2051272/1‑100 (MQ=255)
   tgtgGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTa                                                                                           <  2:5338166/100‑1 (MQ=255)
    gtgGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTAc                                                                                          <  1:200341/100‑1 (MQ=255)
     tgGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACt                                                                                         <  1:3193960/100‑1 (MQ=255)
      ggAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATATACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTg                                                                                        >  2:4991746/1‑100 (MQ=255)
      ggAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTg                                                                                        <  2:5167182/100‑1 (MQ=255)
       gAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGt                                                                                       >  2:2224077/1‑100 (MQ=255)
        aaGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGtt                                                                                      <  1:3533570/100‑1 (MQ=255)
        aaGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGtt                                                                                      >  1:657502/1‑100 (MQ=255)
        aaGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGtt                                                                                      >  1:4869434/1‑100 (MQ=255)
        aaGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGtt                                                                                      >  1:4397119/1‑100 (MQ=255)
         aGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGttt                                                                                     <  2:2584223/100‑1 (MQ=255)
           ccGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTAc                                                                                   >  2:4912664/1‑100 (MQ=255)
               aCTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAAc                                                                               <  1:455374/100‑1 (MQ=255)
                cTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACt                                                                              <  1:2627579/100‑1 (MQ=255)
                cTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACt                                                                              >  2:2310696/1‑100 (MQ=255)
                 tAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTg                                                                             <  2:3493152/100‑1 (MQ=255)
                  aGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTgg                                                                            >  1:2017108/1‑100 (MQ=255)
                  aGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTgg                                                                            >  1:2752355/1‑100 (MQ=255)
                   gACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGc                                                                           <  2:3955875/100‑1 (MQ=255)
                    aCCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACTGGAATTTACTGTTTACCAACTGGCt                                                                          >  1:4624367/1‑100 (MQ=255)
                     cccGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTc                                                                         >  2:285117/1‑100 (MQ=255)
                     cccGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTc                                                                         <  2:3382028/100‑1 (MQ=255)
                     cccGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTc                                                                         <  2:4227725/100‑1 (MQ=255)
                      ccGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCa                                                                        <  2:3820107/100‑1 (MQ=255)
                      ccGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCa                                                                        <  2:3694620/100‑1 (MQ=255)
                      ccGGATGTACCGTATAACTATTCCCCGCTCAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCa                                                                        <  1:5067528/100‑1 (MQ=255)
                       cGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAA‑TTACTGTTTACCAACTGGCTCAAc                                                                      <  2:2769493/100‑1 (MQ=255)
                        ggATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAAc                                                                      >  1:927256/1‑100 (MQ=255)
                        ggATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAAc                                                                      <  1:2440577/100‑1 (MQ=255)
                        ggATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAAc                                                                      <  2:1101944/100‑1 (MQ=255)
                         gATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACt                                                                     <  1:4317732/100‑1 (MQ=255)
                         gATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACt                                                                     <  2:1621832/100‑1 (MQ=255)
                           tGTACCGTATAACTATTGCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTAt                                                                   <  1:3101564/100‑1 (MQ=255)
                              aCCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTAc                                                                <  1:1694649/100‑1 (MQ=255)
                               ccGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACg                                                               <  2:3721200/100‑1 (MQ=255)
                                 gTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTc                                                             >  1:2148736/1‑100 (MQ=255)
                                 gTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTCTTTACCAACTGGCCCATCTCTTACGTc                                                             >  1:4703727/1‑100 (MQ=255)
                                  tataACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCt                                                            <  1:2800300/100‑1 (MQ=255)
                                   ataACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTa                                                           <  2:2726013/100‑1 (MQ=255)
                                   ataACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTa                                                           >  2:4368714/1‑100 (MQ=255)
                                    taACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCttc                                                          >  1:1945623/1‑98 (MQ=255)
                                     aaCTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTAcc                                                         >  2:3126546/1‑100 (MQ=255)
                                     aaCTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGACTCAACTATTACGTCTAcc                                                         >  1:4957479/1‑100 (MQ=255)
                                      aCTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCa                                                        >  1:5473604/1‑100 (MQ=255)
                                      aCTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCa                                                        <  2:3435630/100‑1 (MQ=255)
                                      aCTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCa                                                        >  2:1196482/1‑100 (MQ=255)
                                       cTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAg                                                       >  1:1178056/1‑100 (MQ=255)
                                       cTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAg                                                       <  1:3312348/100‑1 (MQ=255)
                                       cTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAg                                                       >  1:3125184/1‑100 (MQ=255)
                                         aTTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGAt                                                     <  2:3149550/100‑1 (MQ=255)
                                          tttCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATc                                                    <  2:2531776/100‑1 (MQ=255)
                                           ttCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCa                                                   >  2:5355634/1‑100 (MQ=255)
                                             cccGCACAATGATCCGCAAAATACACCGCGAGCGAGCTCGCGTAGTCACGGGAATTTACTGTTTACCAACTTTCTCAACTATTACGTCTACCATATCACg                                                 >  2:3631710/1‑100 (MQ=255)
                                              ccGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGc                                                >  2:788047/1‑100 (MQ=255)
                                              ccGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGc                                                >  2:1384489/1‑100 (MQ=255)
                                              ccGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGc                                                >  1:981794/1‑100 (MQ=255)
                                               cGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGcc                                               <  1:421171/100‑1 (MQ=255)
                                               cGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGcc                                               <  2:4522046/100‑1 (MQ=255)
                                                  acaATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATa                                            <  2:397267/100‑1 (MQ=255)
                                                    aaTGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACg                                          <  1:1882452/100‑1 (MQ=255)
                                                     aTGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGa                                         >  1:5346711/1‑100 (MQ=255)
                                                      tGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGTCATACGAt                                        <  2:5264818/100‑1 (MQ=255)
                                                        aTCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCt                                      >  1:3952702/1‑100 (MQ=255)
                                                         tCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTa                                     <  2:2112615/100‑1 (MQ=255)
                                                          ccGCAAAATACACCGCGAGCGAGCTGTCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTAc                                    <  1:513444/100‑1 (MQ=255)
                                                           cGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTGCGTCTACCAGATCACGCCATACGATCTACg                                   >  1:3588458/1‑100 (MQ=255)
                                                           cGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATTCGATCTACg                                   >  1:2279637/1‑100 (MQ=255)
                                                            gCAAAATACACCACGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACgg                                  >  2:4173244/1‑100 (MQ=255)
                                                               tcttACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACAGcac                               <  2:4682377/97‑1 (MQ=255)
                                                               aaaTACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGcac                               <  2:5047986/100‑1 (MQ=255)
                                                                 aTACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACAt                             <  1:3816378/100‑1 (MQ=255)
                                                                 aTACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACAt                             <  1:1294967/100‑1 (MQ=255)
                                                                 aTACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACAt                             <  2:2359203/100‑1 (MQ=255)
                                                                  tACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATg                            <  2:599438/100‑1 (MQ=255)
                                                                  tACACCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATg                            >  2:1547709/1‑100 (MQ=255)
                                                                    cacCGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGaa                          <  1:4108665/100‑1 (MQ=255)
                                                                      ccGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATTAATc                        >  1:747450/1‑100 (MQ=255)
                                                                      ccGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATc                        >  1:3733504/1‑100 (MQ=255)
                                                                      ccGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATc                        >  1:2097432/1‑100 (MQ=255)
                                                                      ccGCGAGCGAGCTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATc                        <  1:2175692/100‑1 (MQ=255)
                                                                      ccGCGAGCGAGCGTGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATc                        <  2:4924119/100‑1 (MQ=255)
                                                                          gagcgagcTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGGTCACGCCATACGATCTACGGCACATGAATCCAAc                    >  1:1401002/1‑100 (MQ=255)
                                                                             cgagcTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGCATCCAACGCt                 >  2:3900994/1‑100 (MQ=255)
                                                                              gagcTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTg                <  2:2233103/100‑1 (MQ=255)
                                                                                 cTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGAt             <  2:1882039/100‑1 (MQ=255)
                                                                                 cTGGCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGAt             >  2:2971317/1‑100 (MQ=255)
                                                                                   ggCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTa           >  2:145293/1‑100 (MQ=255)
                                                                                   ggCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTa           >  1:3901403/1‑100 (MQ=255)
                                                                                    gCGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAGCGTTGGAttca          <  1:3455385/100‑3 (MQ=255)
                                                                                     cGTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACCTGAATCCAACGCTGGTTTAAt         >  1:2532676/1‑100 (MQ=255)
                                                                                      gTAGTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAAtc        >  2:4887709/1‑100 (MQ=255)
                                                                                         gTCACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATCAAtcttc     <  1:3679021/100‑1 (MQ=255)
                                                                                           naCGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCtg   >  2:2577050/2‑100 (MQ=255)
                                                                                           cACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATTTTCtg   >  1:268936/1‑100 (MQ=255)
                                                                                           cACGGGAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCtg   >  2:292224/1‑100 (MQ=255)
                                                                                            aCGGGAATTTACTGTTTACCCACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCtgt  >  1:4349787/1‑100 (MQ=255)
                                                                                                |                                                                                               
CGATGTGGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGT  >  NC_000913/4215026‑4215217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑