Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,269,654 T→G F81V (TTC→GTC)  aphA → acid phosphatase/phosphotransferase, class B, non‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,269,6540TG97.2% 706.2 / 2.1 219F81V (TTC→GTC) aphAacid phosphatase/phosphotransferase, class B, non‑specific
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base G (112/100);  total (116/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 219 total aligned reads displayed.

GGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTC  >  NC_000913/4269557‑4269749
                                                                                                 |                                                                                               
 gTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCt                                                                                              <  2:1017159/100‑1 (MQ=255)
 gTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCt                                                                                              >  2:3259127/1‑100 (MQ=255)
  tttCGGTCGCACAAATTGAAAATAGCCTCGCAGGTCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTg                                                                                             <  1:2781399/100‑1 (MQ=255)
  tttCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTg                                                                                             >  2:1344334/1‑100 (MQ=255)
     cGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGGGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGGCTGgcg                                                                                          >  1:2695669/1‑100 (MQ=255)
      ggTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGgcgc                                                                                         <  1:1232875/100‑1 (MQ=255)
       ntCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGcgcg                                                                                        >  1:4438797/2‑100 (MQ=255)
       gTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGcgcg                                                                                        >  1:45434/1‑100 (MQ=255)
           cacaAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCaa                                                                                    >  2:4211348/1‑100 (MQ=255)
            acaAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCaaa                                                                                   <  2:321245/100‑1 (MQ=255)
             caAGTTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTACAGTCCGGGCGTCTGCCGCGGCaaaa                                                                                  >  2:4061/1‑100 (MQ=255)
             caAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCaaaa                                                                                  >  2:773141/1‑100 (MQ=255)
             caAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCaaaa                                                                                  >  1:3716929/1‑100 (MQ=255)
             caAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCaaaa                                                                                  >  2:5393103/1‑100 (MQ=255)
              aaaTTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCaaaaa                                                                                 >  1:306397/1‑100 (MQ=255)
               aaTTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCaaaaaa                                                                                >  1:5161105/1‑100 (MQ=255)
               aaTTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCaaaaaa                                                                                >  1:3851407/1‑100 (MQ=255)
               aaTTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCaaaaaa                                                                                >  1:1150583/1‑100 (MQ=255)
                aTTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATCTCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCaaaaaaa                                                                               >  2:3768004/1‑100 (MQ=255)
                aTTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCaaaaaaa                                                                               <  1:4674263/100‑1 (MQ=255)
                aTTGAAAATAGCCTCGCAGGGCGACCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCATTCCGGGCGTGTGGCGCGGCaaaaaaa                                                                               >  2:4609999/1‑100 (MQ=255)
                 ttGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAAc                                                                              <  2:488974/100‑1 (MQ=255)
                  tGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAAcc                                                                             >  2:3895109/1‑100 (MQ=255)
                   gAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTATTTCCAGTCCAGGCTTCTGGCGCGGCAAAAAAACCt                                                                            >  2:5198114/1‑100 (MQ=255)
                    aaaaTAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGGTTGGCGCGGCAAAAAAACCtt                                                                           >  2:3957524/1‑100 (MQ=255)
                     aaaTCGCCTCGCAGGGCGTCCGCCCATGGCGGCGGGGTTTGCTATCGATGACACGGTACTTTTTTCCAGTACGGGCCTCCAGTGCGGCCAAAAACCCtcc                                                                          >  2:4328407/1‑98 (MQ=255)
                     aaaTAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTtc                                                                          <  1:344434/100‑1 (MQ=255)
                     aaaTAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTtc                                                                          >  1:3191827/1‑100 (MQ=255)
                     aaaTAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTtc                                                                          >  1:5468497/1‑100 (MQ=255)
                     aaaTAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTtc                                                                          <  1:4780395/100‑1 (MQ=255)
                     aaaTAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTtc                                                                          >  2:3098182/1‑100 (MQ=255)
                       aTAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTtctc                                                                        <  1:669932/100‑1 (MQ=255)
                          gCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGcc                                                                     <  2:1251173/100‑1 (MQ=255)
                            cTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAg                                                                   <  1:2123993/100‑1 (MQ=255)
                             tCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGa                                                                  >  2:223617/1‑100 (MQ=255)
                                 aGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGc                                                              >  2:2989217/1‑100 (MQ=255)
                                   ggCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGa                                                            >  1:3805346/1‑100 (MQ=255)
                                   ggCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGa                                                            >  1:4270131/1‑100 (MQ=255)
                                    gCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGaa                                                           <  1:1364421/100‑1 (MQ=255)
                                    gCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGaa                                                           <  2:4396774/100‑1 (MQ=255)
                                    gCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGaa                                                           >  1:406937/1‑100 (MQ=255)
                                     cGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAg                                                          <  2:5006595/100‑1 (MQ=255)
                                      gTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGa                                                         <  1:2424209/100‑1 (MQ=255)
                                         cGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTTTTTTTCTTGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGGAAGCGAAGATTa                                                      >  2:1421501/1‑100 (MQ=255)
                                         cGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTa                                                      >  2:762554/1‑100 (MQ=255)
                                         cGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTa                                                      >  2:3507512/1‑100 (MQ=255)
                                         cGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTAAAGAAAGAAAAAAAAACCTTCTCGCCAGAAAGCGAAGATTa                                                      <  2:1641585/100‑1 (MQ=255)
                                           cggtaggCAGTGGGGTCTGAAATCGAAGAAACGATACTTATTTCGAGTACAGGGTTATGGGAAGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATc                                                    <  2:391777/95‑1 (MQ=255)
                                           ccAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATc                                                    <  2:2628815/100‑1 (MQ=255)
                                            tgggggCGGTGGGGGTGGATATCGATGACAGGGTACTTTCTTGCGGTGCGGGTGTCTGGCTAGGCAGAAAGACCTTCTCGCCAGAAAGCGAAGATTATCt                                                   <  2:4759959/96‑1 (MQ=255)
                                            cAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCt                                                   <  2:1763849/100‑1 (MQ=255)
                                             aaTGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTg                                                  >  1:2617568/1‑100 (MQ=255)
                                              aTGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGa                                                 >  1:3310085/1‑100 (MQ=255)
                                               tGGCGGTGGGGTGTGATATCGATGACACGGTACTTTTTTCGAGTCGGGGGGGCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaa                                                <  2:4917287/100‑1 (MQ=255)
                                                ggCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaaa                                               <  1:501041/100‑1 (MQ=255)
                                                     tGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATcc                                          <  1:1511124/100‑1 (MQ=255)
                                                     tGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATcc                                          <  1:1655650/100‑1 (MQ=255)
                                                     tGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTAAAGAAAAAAAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATcc                                          <  2:614586/100‑1 (MQ=255)
                                                       gggTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCtg                                        <  1:463680/100‑1 (MQ=255)
                                                       gggTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCtg                                        >  1:3787284/1‑100 (MQ=255)
                                                       ggaattGAAATCGAAGAAACGGTACTTTTTTCCAGTCCGGGCGAAAAAAAAAAAAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCtg                                        <  2:3000524/96‑1 (MQ=255)
                                                        ggTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCtgt                                       >  1:83578/1‑100 (MQ=255)
                                                            tGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAACCAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTc                                   >  2:1496292/1‑100 (MQ=255)
                                                            tGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTc                                   <  2:3727486/100‑1 (MQ=255)
                                                            tGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTc                                   <  1:1864105/100‑1 (MQ=255)
                                                             gATATCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCt                                  >  1:4142121/1‑100 (MQ=255)
                                                              atatCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTg                                 >  2:3369530/1‑100 (MQ=255)
                                                              atatCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTg                                 >  1:1063513/1‑100 (MQ=255)
                                                                atCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTggg                               <  2:3539128/100‑1 (MQ=255)
                                                                atCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTggg                               >  2:5346205/1‑100 (MQ=255)
                                                                atCGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTggg                               >  1:834192/1‑100 (MQ=255)
                                                                  cGATGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGaa                             >  1:1971728/1‑100 (MQ=255)
                                                                    aTGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGaaaa                           >  2:1852600/1‑100 (MQ=255)
                                                                     tGACACGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGaaaaa                          >  2:657646/1‑100 (MQ=255)
                                                                       acacGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAAt                        >  2:1940357/1‑100 (MQ=255)
                                                                         acGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGa                      <  2:4837575/100‑1 (MQ=255)
                                                                          cGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGCAAAACTGaa                     >  1:4189124/1‑100 (MQ=255)
                                                                          cGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGaa                     <  2:3697817/100‑1 (MQ=255)
                                                                          cGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGaa                     <  1:30828/100‑1 (MQ=255)
                                                                          cGGTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTAGCTGAAAAATCCTGTGTTCTGGGAAAAAATGaa                     <  2:5330027/100‑1 (MQ=255)
                                                                           ggTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAAc                    <  1:727193/100‑1 (MQ=255)
                                                                           ggTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAAc                    <  2:468973/100‑1 (MQ=255)
                                                                           ggTACTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAAc                    <  2:824856/100‑1 (MQ=255)
                                                                           ggTACTTTTTTCCAGTCCGGGCGTAAAAAAAAAAAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAAc                    <  2:339588/100‑1 (MQ=255)
                                                                              aCTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAAt                 <  1:1012902/100‑1 (MQ=255)
                                                                               cTTTTTTCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGTAGGATATCTGAAAAATGCTGTGTTCTGGGAAAAAATGAACAATg                >  2:5248863/1‑100 (MQ=255)
                                                                                ttttttCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATgg               <  1:4170885/100‑1 (MQ=255)
                                                                                 ttttaCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCTAAGATTATCTGAAAAATCCTGCGCTCTGGGAAAAAATGAACAATGGc              <  1:4986085/100‑1 (MQ=255)
                                                                                    gatctgTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTgg           <  1:1847565/95‑1 (MQ=255)
                                                                                     tCCAGTCCGGGCGTCTGGCGCGGCAAAAAACCCTTCACGCCAGAAAGCGAAGATTATCTGAAAAATCCTAAAAAAAAAAAAAAAATGAACAATGGCTggg          <  2:2669653/100‑1 (MQ=255)
                                                                                     tCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTggg          <  1:3616728/100‑1 (MQ=255)
                                                                                     tCCAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTggg          <  1:5293609/100‑1 (MQ=255)
                                                                                      ccAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGa         >  2:394350/1‑100 (MQ=255)
                                                                                      ccAGTCCAGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGa         >  2:3173790/1‑100 (MQ=255)
                                                                                       gaGTCGGGGCGTAAGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGATAAATCCTAAAAAAAAAAAAAAAATGAACAATGGCTGGGAt        <  2:3883652/99‑1 (MQ=255)
                                                                                       cAGTCCGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGAt        >  1:5374570/1‑100 (MQ=255)
                                                                                        agaccGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAACCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATg       <  1:1266877/97‑1 (MQ=255)
                                                                                           ccGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAAt    >  2:1098643/1‑100 (MQ=255)
                                                                                            cGGGCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAAtt   <  1:2486281/100‑1 (MQ=255)
                                                                                             gggCGTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTc  <  1:652278/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTC  >  NC_000913/4269557‑4269749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑