Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,280,134 T→G F52C (TTT→TGT)  yjcE → putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,280,1340TG97.8% 609.7 / 1.6 178F52C (TTT→TGT) yjcEputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base G (79/95);  total (82/96)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

Only 100 of 179 total aligned reads displayed.

TCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAG  >  NC_000913/4280038‑4280229
                                                                                                |                                                                                               
tCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGa                                                                                              <  2:4628113/100‑1 (MQ=255)
tCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGa                                                                                              <  2:4627921/100‑1 (MQ=255)
 cACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGAt                                                                                             <  2:1933714/100‑1 (MQ=255)
   cTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATcc                                                                                           <  1:5464842/100‑1 (MQ=255)
   cTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATcc                                                                                           <  1:5294695/100‑1 (MQ=255)
   cTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATcc                                                                                           <  2:1463216/100‑1 (MQ=255)
   cTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATcc                                                                                           <  2:2308395/100‑1 (MQ=255)
   cTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATcc                                                                                           <  2:2794848/100‑1 (MQ=255)
     cGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTg                                                                                         >  2:2895202/1‑100 (MQ=255)
     cGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTg                                                                                         <  1:2099200/100‑1 (MQ=255)
      ntgtCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGa                                                                                        >  1:4438943/2‑100 (MQ=255)
      gtgtCATGCCCTTTCATATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGa                                                                                        <  1:4590438/100‑1 (MQ=255)
      gtgtCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGa                                                                                        <  1:785314/100‑1 (MQ=255)
       tgtCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGaa                                                                                       <  2:5136714/100‑1 (MQ=255)
       tgtCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGaa                                                                                       >  1:476856/1‑100 (MQ=255)
        gtCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAAc                                                                                      <  1:5521696/100‑1 (MQ=255)
        gtCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAAc                                                                                      <  1:355178/100‑1 (MQ=255)
           aTGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGaaaggg                                                                                   >  1:2800946/1‑96 (MQ=255)
           aTGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAActct                                                                                   >  2:194108/1‑100 (MQ=255)
              cccTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCtttt                                                                                >  1:5148115/1‑100 (MQ=255)
              cccTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCtttt                                                                                <  2:2989685/100‑1 (MQ=255)
              cccTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCtttt                                                                                >  1:4849842/1‑100 (MQ=255)
               ccTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCttttt                                                                               <  2:3067234/100‑1 (MQ=255)
               ccTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCttttt                                                                               <  1:3767115/100‑1 (MQ=255)
               ccTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCttttt                                                                               >  1:1534616/1‑100 (MQ=255)
                cTTTCAGATCCCGCTTTCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTTATCCTGAACTCTTTTTa                                                                              <  1:2259113/100‑1 (MQ=255)
                 tttCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAg                                                                             <  1:5329914/100‑1 (MQ=255)
                  ttCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGt                                                                            <  2:50011/100‑1 (MQ=255)
                  ttCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGt                                                                            <  2:787337/100‑1 (MQ=255)
                   tCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTc                                                                           <  1:3308359/100‑1 (MQ=255)
                   tCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTc                                                                           <  1:3849376/100‑1 (MQ=255)
                    cAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCt                                                                          <  2:3300108/100‑1 (MQ=255)
                      gATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGCCGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTg                                                                        >  1:4348525/1‑100 (MQ=255)
                      gATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTg                                                                        >  1:4248028/1‑100 (MQ=255)
                       aTCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGt                                                                       <  1:4389219/100‑1 (MQ=255)
                       aTCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGt                                                                       >  2:631098/1‑100 (MQ=255)
                        tCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGtt                                                                      <  2:579621/100‑1 (MQ=255)
                        tCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGtt                                                                      <  2:3679610/100‑1 (MQ=255)
                        tCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGtt                                                                      <  2:3944840/100‑1 (MQ=255)
                         cccGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGttt                                                                     <  2:5441584/100‑1 (MQ=255)
                           cgcttccgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTAt                                                                   <  2:3698922/100‑1 (MQ=255)
                           cgcttccgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTAt                                                                   <  1:4075366/100‑1 (MQ=255)
                            gcttccgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATc                                                                  >  1:970686/1‑100 (MQ=255)
                            gcttccgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATc                                                                  <  2:1869005/100‑1 (MQ=255)
                             cttccgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATcc                                                                 <  1:1606231/100‑1 (MQ=255)
                              ttccgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATccc                                                                <  2:978910/100‑1 (MQ=255)
                                 cgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCccgcc                                                             >  2:1212805/1‑100 (MQ=255)
                                  gcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGCGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCccgccg                                                            <  2:4164412/100‑1 (MQ=255)
                                  gcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCccgccg                                                            >  1:3736262/1‑100 (MQ=255)
                                  gcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCccgccg                                                            <  1:4476311/100‑1 (MQ=255)
                                      aTGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTTGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGc                                                        <  2:2445755/100‑1 (MQ=255)
                                      aTGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGc                                                        <  1:2567314/100‑1 (MQ=255)
                                       tGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCt                                                       >  1:1976573/1‑100 (MQ=255)
                                       tGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCt                                                       >  2:4228210/1‑100 (MQ=255)
                                        gCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTg                                                      >  1:1022219/1‑100 (MQ=255)
                                        gCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTg                                                      >  1:4530235/1‑100 (MQ=255)
                                         cAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGt                                                     <  2:41387/100‑1 (MQ=255)
                                         cAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGt                                                     >  1:3282493/1‑100 (MQ=255)
                                          aaaTCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGtt                                                    >  2:3377150/1‑100 (MQ=255)
                                           aaTCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTc                                                   >  1:1040681/1‑100 (MQ=255)
                                           aaTCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTc                                                   <  1:2245374/100‑1 (MQ=255)
                                            aTCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCg                                                  >  1:874512/1‑100 (MQ=255)
                                               gCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTg                                               <  1:2049482/100‑1 (MQ=255)
                                                 cATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGAt                                             >  1:1887161/1‑100 (MQ=255)
                                                  aTCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATg                                            >  1:1962085/1‑100 (MQ=255)
                                                  aTCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATg                                            >  2:1842322/1‑100 (MQ=255)
                                                    cGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGc                                          >  1:683829/1‑100 (MQ=255)
                                                      gTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTg                                        >  2:47710/1‑100 (MQ=255)
                                                      gTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTg                                        <  2:2244227/100‑1 (MQ=255)
                                                       tGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTgg                                       <  2:1421232/100‑1 (MQ=255)
                                                        gcgcTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGa                                      <  2:3983082/100‑1 (MQ=255)
                                                         cgcTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGaa                                     >  1:1400929/1‑100 (MQ=255)
                                                         cgcTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGaa                                     >  2:23116/1‑100 (MQ=255)
                                                         cgcTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGGGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGaa                                     <  2:1324374/100‑1 (MQ=255)
                                                            tACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAAc                                  >  2:4822006/1‑100 (MQ=255)
                                                              cTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGc                                >  1:2158753/1‑100 (MQ=255)
                                                               tGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGcc                               <  2:2670419/100‑1 (MQ=255)
                                                                ggCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCg                              <  2:863005/100‑1 (MQ=255)
                                                                ggCGTGGCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCg                              <  2:3464986/100‑1 (MQ=255)
                                                                      gCCGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGt                        >  1:5054969/1‑100 (MQ=255)
                                                                       ccGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTg                       <  1:3481339/100‑1 (MQ=255)
                                                                       ccGACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTg                       <  1:1135731/100‑1 (MQ=255)
                                                                         gACGTTTGGTTTGCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGaa                     >  1:4717183/1‑100 (MQ=255)
                                                                             tttggtttgCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGTAAACGCCGCCCCGTGAATTtc                 >  2:4352336/1‑100 (MQ=255)
                                                                              ttggtttgCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTtct                >  2:2540729/1‑100 (MQ=255)
                                                                               tggtttgCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTtctc               <  1:48223/100‑1 (MQ=255)
                                                                               tggtttgCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTtctc               <  2:3084286/100‑1 (MQ=255)
                                                                               tggtttgCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTtctc               <  2:2659465/100‑1 (MQ=255)
                                                                                ggtttgCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCTTTGCTGTTCGCTGATGGCTGGAAACCGCCGACCCGTGAATTTCTCg              <  1:1449273/100‑1 (MQ=255)
                                                                                ggtttgCATGTGGAGTGTGATCCTGAACTCTATTTAGTCTTGTTTATCCCGCCGTCCATGTTCGCTGATGCCTGGATAACGCCGGCCCGTGAGTTTCTCg              >  2:5216342/1‑100 (MQ=255)
                                                                                 gtttgCATGTGGAGGGTGATCATGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCCGGAAAACGCCGACCCGTGAATTTCTCgg             >  2:151681/1‑99 (MQ=255)
                                                                                   ttgCATGTGGAGTGTGATCCTGAACTCTTTTTTTTTTTTTTTATCCCGCCGTTGCTGTTCGCTGATTTCTGGAAATTGCCGACCCGTGAATTtctcttac           >  2:2217423/1‑96 (MQ=255)
                                                                                   ttgCATGTGGAGTGGGATCCTGAACTATTTTTAGTCTTGTTTATCCCGCCGTTGATGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAAc           >  2:5137541/1‑100 (MQ=255)
                                                                                    tgCATGTGGAGTGTGCTCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACa          <  1:1800702/100‑1 (MQ=255)
                                                                                    tgCATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACa          <  1:4502713/100‑1 (MQ=255)
                                                                                      cATGTGGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATg        >  1:4884392/1‑100 (MQ=255)
                                                                                        tgtgGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGt      >  1:3081305/1‑100 (MQ=255)
                                                                                        tgtgGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGt      >  2:4461532/1‑100 (MQ=255)
                                                                                         gtgGAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTc     <  2:2557756/100‑1 (MQ=255)
                                                                                           ggAGTGTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCga   >  2:59495/1‑100 (MQ=255)
                                                                                                |                                                                                               
TCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAG  >  NC_000913/4280038‑4280229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: