BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,285,713	A→C	N553K (AAT→AAG)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,713	0	A	C	97.7%	769.7 / 1.3	221	N553K (AAT→AAG)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (2/3); new base C (103/111); total (105/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

CTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC  >  NC_000913/4285616‑4285808

 tttACGCACCCAGTTGCGGACTTCTTTGTACAGTTCTGGTGACGGTTCCACCCCGTGATTAAGCGTGACGTAGGCGTAGATCTCCTGACCTTTAATCTtg                                                                                              <  2:778925/100‑1 (MQ=255)

 tttACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTtg                                                                                              <  2:588962/100‑1 (MQ=255)

  ttACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTtgt                                                                                             <  1:1044592/100‑1 (MQ=255)

  ttACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTtgt                                                                                             >  2:283120/1‑100 (MQ=255)

  ttACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTtgt                                                                                             >  1:2648409/1‑100 (MQ=255)

   tACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTtgtg                                                                                            <  2:1959172/100‑1 (MQ=255)

   tACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTtgtg                                                                                            <  2:3065119/100‑1 (MQ=255)

   tACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTtgtg                                                                                            <  2:4207663/100‑1 (MQ=255)

    aCGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGc                                                                                           <  1:911557/100‑1 (MQ=255)

    aCGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGc                                                                                           >  1:1394993/1‑100 (MQ=255)

     cGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCg                                                                                          >  2:4037866/1‑100 (MQ=255)

      gctcccAGTGGCGGACTTCTGCGTACAGTTCTGGTGACGGATCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATGTTGTGCgg                                                                                         <  2:5268325/97‑1 (MQ=255)

      gCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCgg                                                                                         <  2:3295068/100‑1 (MQ=255)

      gCACCCAGTTGCGGACTTCTGCGTACACTTCTGGTGACGGTTCCTCCCCGTGATGAAGCGTGACGTAGGCGTAGATCGCCTGACGTTTAATCTTGTGCgg                                                                                         <  1:4834020/100‑1 (MQ=255)

       cACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGa                                                                                        <  1:2594782/100‑1 (MQ=255)

        aCCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGaa                                                                                       >  2:1529818/1‑100 (MQ=255)

         cccAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAAt                                                                                      <  1:2304769/100‑1 (MQ=255)

         cccAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAAt                                                                                      <  2:1123424/100‑1 (MQ=255)

         cccAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAAt                                                                                      <  2:1247215/100‑1 (MQ=255)

           cAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTGAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATAc                                                                                    <  1:5282822/100‑1 (MQ=255)

            aGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATAcc                                                                                   <  2:4041244/100‑1 (MQ=255)

            aGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATAcc                                                                                   <  1:335713/100‑1 (MQ=255)

             gTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACct                                                                                  >  2:1081826/1‑100 (MQ=255)

               tGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGTTTAAGCGTGACGTAGGCGTCGACCGCCTGCCCTTTAATCTTGTGCGGAATACctac                                                                                >  2:1694394/1‑100 (MQ=255)

               tGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACctac                                                                                <  2:5187266/100‑1 (MQ=255)

                gCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACctact                                                                               <  2:960416/100‑1 (MQ=255)

                gCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACctact                                                                               <  1:2135478/100‑1 (MQ=255)

                 cGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACctacta                                                                              >  1:1156156/1‑100 (MQ=255)

                     cTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTAcggc                                                                          >  2:3710011/1‑100 (MQ=255)

                      ttCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTAcggcg                                                                         >  2:4701970/1‑100 (MQ=255)

                       tCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTAcggcgg                                                                        <  2:2182758/100‑1 (MQ=255)

                       tCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTAcggcgg                                                                        >  2:1383732/1‑100 (MQ=255)

                            gTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCg                                                                   >  2:3789143/1‑100 (MQ=255)

                             tACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCgg                                                                  <  1:3410081/100‑1 (MQ=255)

                             tACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCgg                                                                  >  1:299936/1‑100 (MQ=255)

                               cAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCa                                                                >  1:1359096/1‑100 (MQ=255)

                                aGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCaa                                                               >  1:549584/1‑100 (MQ=255)

                                 gTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAAt                                                              <  1:991702/100‑1 (MQ=255)

                                 gTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAAt                                                              >  1:1209752/1‑100 (MQ=255)

                                   tCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCt                                                            <  1:1698828/100‑1 (MQ=255)

                                   tCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCt                                                            <  1:4723014/100‑1 (MQ=255)

                                    cTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCtt                                                           >  1:4374923/1‑100 (MQ=255)

                                     tGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTc                                                          <  2:417225/100‑1 (MQ=255)

                                      ggTGACGGTTCCTCCCCGTGATTAAGCGTTACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCg                                                         <  2:1863656/100‑1 (MQ=255)

                                      ggTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCg                                                         >  2:720141/1‑100 (MQ=255)

                                      ggTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCg                                                         <  1:1457264/100‑1 (MQ=255)

                                      ggTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCg                                                         >  2:2942042/1‑100 (MQ=255)

                                        tGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGa                                                       >  1:117628/1‑100 (MQ=255)

                                           cGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgc                                                    >  1:2817587/1‑100 (MQ=255)

                                           cGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgc                                                    <  1:4152047/100‑1 (MQ=255)

                                                cctccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGccac                                               <  2:2371912/100‑1 (MQ=255)

                                                 ctccCCGTGATTAAGCGTGACGTAGGTGTAGATCACCGGGGCTTCAATATTGTGCAGAATACCTACTACGGCTACTCCGGCAATCTTAGGATGCGccacc                                              >  2:5082775/1‑100 (MQ=255)

                                                 ctccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGccacc                                              >  2:3395410/1‑100 (MQ=255)

                                                 ctccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGccacc                                              >  1:3430390/1‑100 (MQ=255)

                                                 ctccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGccacc                                              >  1:5405777/1‑100 (MQ=255)

                                                   ccccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAg                                            >  1:3204786/1‑100 (MQ=255)

                                                    cccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAgc                                           >  1:726645/1‑100 (MQ=255)

                                                    cccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAgc                                           >  1:5158520/1‑100 (MQ=255)

                                                    cccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAgc                                           <  1:5002300/100‑1 (MQ=255)

                                                     ccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAgcg                                          >  1:3795730/1‑100 (MQ=255)

                                                       gTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGcc                                        <  1:148087/100‑1 (MQ=255)

                                                       gTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGcc                                        <  2:1341181/100‑1 (MQ=255)

                                                       gTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGcc                                        >  1:2729222/1‑100 (MQ=255)

                                                        tGATTAAGCGTGACGTAGGCGTATATCGCCTGACCCTTACTCTTCTGCGGAATACCTACAACGGCTGCGTCTGCAATTTGCTGATGCACCTCCAGCGCCg                                       >  2:3308833/1‑100 (MQ=255)

                                                        tGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCg                                       <  2:3206382/100‑1 (MQ=255)

                                                         naTTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGa                                      >  1:4411397/2‑100 (MQ=255)

                                                         gATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGa                                      >  2:4251952/1‑100 (MQ=255)

                                                         gATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGa                                      >  1:3570751/1‑100 (MQ=255)

                                                          aTTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGAc                                     <  2:1467470/100‑1 (MQ=255)

                                                                 gTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAAtct                              >  1:3095110/1‑100 (MQ=255)

                                                                  tGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAAtctn                             <  2:4364034/100‑2 (MQ=255)

                                                                   gACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATctct                            <  2:2562417/100‑1 (MQ=255)

                                                                    aCGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGGTGCGCCACCAGCGCCGACTCAATCTCTg                           >  1:4260446/1‑100 (MQ=255)

                                                                    aCGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTg                           <  2:374667/100‑1 (MQ=255)

                                                                    aCGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTg                           <  1:4775996/100‑1 (MQ=255)

                                                                    aCGTAGGCGTAGATCGCCTGACCTTTAATCTTGGGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTg                           <  1:2782425/100‑1 (MQ=255)

                                                                     cGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATAGCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGc                          <  2:4909016/100‑1 (MQ=255)

                                                                      gTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGcc                         <  1:5350878/100‑1 (MQ=255)

                                                                       tAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCg                        <  1:4008132/100‑1 (MQ=255)

                                                                          gCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTcc                     >  1:1306360/1‑100 (MQ=255)

                                                                           cGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTccc                    >  2:11665/1‑100 (MQ=255)

                                                                            gTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTcccc                   <  1:1096397/100‑1 (MQ=255)

                                                                            gTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTcccc                   <  2:3123383/100‑1 (MQ=255)

                                                                            gTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTcccc                   <  2:4773213/100‑1 (MQ=255)

                                                                               gATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGa                >  1:5037682/1‑100 (MQ=255)

                                                                               gATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGa                >  1:1806761/1‑100 (MQ=255)

                                                                               gATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGa                >  2:3191214/1‑100 (MQ=255)

                                                                                 tCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACg              >  1:4700652/1‑100 (MQ=255)

                                                                                   gCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGt            >  2:397492/1‑100 (MQ=255)

                                                                                    ccTGACCTTTAATCTTGTGCGGAATACCTACTACGTCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTg           <  2:4636634/100‑1 (MQ=255)

                                                                                    ccTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTg           >  1:4590393/1‑100 (MQ=255)

                                                                                      tGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGAc         >  1:2002506/1‑100 (MQ=255)

                                                                                      tGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGAc         <  1:2423160/100‑1 (MQ=255)

                                                                                       gACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGAcc        <  2:1193205/100‑1 (MQ=255)

                                                                                         ccTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCgg      >  2:812645/1‑100 (MQ=255)

                                                                                          cTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGga     >  2:3142182/1‑100 (MQ=255)

                                                                                           tttAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGgag    <  1:1936584/100‑1 (MQ=255)

                                                                                           tttAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGgag    <  2:3761653/100‑1 (MQ=255)

                                                                                            ttAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGgaga   <  1:3744397/100‑1 (MQ=255)

                                                                                            ttAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGgaga   <  2:2634901/100‑1 (MQ=255)

CTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC  >  NC_000913/4285616‑4285808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`