Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,291,339 T→G I288M (ATT→ATG nrfD → formate‑dependent nitrite reductase, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,291,3390TG96.9% 781.4 / 8.0 229I288M (ATT→ATGnrfDformate‑dependent nitrite reductase, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (4/3);  new base G (114/107);  total (119/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

GCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAA  >  NC_000913/4291242‑4291432
                                                                                                 |                                                                                             
gCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGcc                                                                                             <  2:1099672/100‑1 (MQ=255)
gCGGTTTCGGGACGTGGTGGTTCTGGCTGGGTGGCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGcc                                                                                             <  1:183255/100‑1 (MQ=255)
 cGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCt                                                                                            >  1:371268/1‑100 (MQ=255)
 cGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCt                                                                                            <  2:1223527/100‑1 (MQ=255)
  ggTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTg                                                                                           <  1:3141326/100‑1 (MQ=255)
    tttCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCAtgcctgcc                                                                                         <  2:3684820/100‑1 (MQ=255)
       cTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTg                                                                                      <  1:5390932/100‑1 (MQ=255)
       cTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTg                                                                                      >  2:2031403/1‑100 (MQ=255)
        tGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGc                                                                                     <  1:1867684/100‑1 (MQ=255)
         ggACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCt                                                                                    >  1:1652011/1‑100 (MQ=255)
          gACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTg                                                                                   >  2:1742412/1‑100 (MQ=255)
          gACGTGGTGGTTCTGGCCTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAAAGCTGCTCAAAACGTGGGTCAATCGCAGTTCCGGCATGTCTGCCCTGCtc                                                                                   >  2:3005393/1‑99 (MQ=255)
            cGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggc                                                                                 >  2:4288672/1‑100 (MQ=255)
            cGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggc                                                                                 <  1:2226357/100‑1 (MQ=255)
             gtggtgGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggcg                                                                                <  1:1081733/100‑1 (MQ=255)
              tggtggTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggcgg                                                                               <  1:388555/100‑1 (MQ=255)
               ggtggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggcggc                                                                              >  1:2764938/1‑100 (MQ=255)
               ggtggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggcggc                                                                              >  2:2636429/1‑100 (MQ=255)
                  ggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCgtg                                                                           <  1:847639/100‑1 (MQ=255)
                  ggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCgtg                                                                           <  1:688294/100‑1 (MQ=255)
                  ggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCgtg                                                                           >  2:477392/1‑100 (MQ=255)
                  ggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCgtg                                                                           <  1:3654821/100‑1 (MQ=255)
                  ggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCgtg                                                                           >  1:3541292/1‑100 (MQ=255)
                   gtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCgtgt                                                                          <  2:4128958/100‑1 (MQ=255)
                      cTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTggg                                                                       >  1:5054439/1‑100 (MQ=255)
                      cTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTggg                                                                       >  2:3032796/1‑100 (MQ=255)
                       tGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTgggg                                                                      <  1:2042515/100‑1 (MQ=255)
                          cTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCa                                                                   >  1:4638932/1‑100 (MQ=255)
                          cTTGGTGTCACCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCa                                                                   >  2:2880312/1‑100 (MQ=255)
                            tGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGt                                                                 >  1:1162974/1‑100 (MQ=255)
                            tGGTGTCGCCGGGCTGGGACTGATTGGGGCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGTCAGt                                                                 <  2:4656543/100‑1 (MQ=255)
                             ggTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTc                                                                <  1:2581042/100‑1 (MQ=255)
                              gtgtCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGtgt                                                               >  2:108300/1‑98 (MQ=255)
                               tgtCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTg                                                              >  1:1764184/1‑100 (MQ=255)
                               tgtCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTg                                                              >  1:3936796/1‑100 (MQ=255)
                                gtCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTgg                                                             >  1:1115924/1‑100 (MQ=255)
                                gtCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTgg                                                             >  1:2720734/1‑100 (MQ=255)
                                  cGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTc                                                           >  2:2145048/1‑100 (MQ=255)
                                  cGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTc                                                           <  1:4164704/100‑1 (MQ=255)
                                  cGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTc                                                           >  1:2861411/1‑100 (MQ=255)
                                   gCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCg                                                          <  1:5307269/100‑1 (MQ=255)
                                      gggctggggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCg                                                       <  2:490602/100‑1 (MQ=255)
                                      gggctggggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCg                                                       <  1:2394546/100‑1 (MQ=255)
                                       ggctggggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCgt                                                      >  1:5519415/1‑100 (MQ=255)
                                        gctggggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCgtg                                                     <  2:4719383/100‑1 (MQ=255)
                                           ggggctgCTTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTGCCGGCATGGCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTg                                                  <  2:3138323/100‑1 (MQ=255)
                                            gggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGa                                                 <  1:3033684/100‑1 (MQ=255)
                                             ggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAt                                                <  2:628139/100‑1 (MQ=255)
                                             ggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAt                                                >  2:1323513/1‑100 (MQ=255)
                                             ggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAt                                                >  1:2534924/1‑100 (MQ=255)
                                             ggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGCGCCAGTCTGGTCGGCGTGTTGAt                                                >  2:2849581/1‑100 (MQ=255)
                                               ctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAtgc                                              >  1:4226728/1‑100 (MQ=255)
                                                tgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAtgct                                             <  1:320419/100‑1 (MQ=255)
                                                 gATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAtgctg                                            >  2:2928293/1‑100 (MQ=255)
                                                  aTTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAtgctgc                                           <  2:258278/100‑1 (MQ=255)
                                                    tgtgCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTgcgc                                         >  2:3427564/1‑100 (MQ=255)
                                                     gtgCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCt                                        <  2:2802424/100‑1 (MQ=255)
                                                      tgCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCtt                                       <  1:3269104/100‑1 (MQ=255)
                                                       gCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCttt                                      <  2:1266851/100‑1 (MQ=255)
                                                       gCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCttt                                      <  1:1131041/100‑1 (MQ=255)
                                                         cAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCttttt                                    >  1:557938/1‑100 (MQ=255)
                                                         cAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCttttt                                    >  2:2110147/1‑100 (MQ=255)
                                                         cAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCttttt                                    <  1:4996288/100‑1 (MQ=255)
                                                         cAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCttttt                                    <  1:1893390/100‑1 (MQ=255)
                                                         cAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCttttt                                    <  1:4712171/100‑1 (MQ=255)
                                                          aaTGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTc                                   <  1:4881415/100‑1 (MQ=255)
                                                           aTGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCa                                  >  1:1349971/1‑100 (MQ=255)
                                                             gctgctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCAtt                                >  2:355054/1‑100 (MQ=255)
                                                             cgatctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCAtt                                <  1:1530483/96‑1 (MQ=255)
                                                              ctgctCGAACCGGGGGTCAATCGCAGTTCCGGCATGCCTGGCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATtc                               <  2:2499977/100‑1 (MQ=255)
                                                              ctgctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATtc                               >  2:713261/1‑100 (MQ=255)
                                                              ctgctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATtc                               <  2:3972712/100‑1 (MQ=255)
                                                              ctgctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATtc                               <  1:4401760/100‑1 (MQ=255)
                                                              ctgctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATtc                               >  1:557045/1‑100 (MQ=255)
                                                               tgctCAAACCGTGGGTCAATCGCCGTTGCGGCATGCCTGCCGTCCTGGCAAGATGTGGTGCCACTCTGTTCGGCGTGTTTATGCTGCTCTTTTACATtct                              <  2:1029993/100‑1 (MQ=255)
                                                                gctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATtctc                             >  1:1042906/1‑100 (MQ=255)
                                                                gctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATtctc                             >  1:3332007/1‑100 (MQ=255)
                                                                gctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATtctc                             >  1:3820078/1‑100 (MQ=255)
                                                                 ctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTctct                            >  2:4326124/1‑100 (MQ=255)
                                                                  tCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTa                           >  2:3369380/1‑100 (MQ=255)
                                                                   cAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTAc                          >  2:1934974/1‑100 (MQ=255)
                                                                     aaCCGTGGGTCAATCGCAGTTCCGGCATACCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGc                        >  2:5086737/1‑100 (MQ=255)
                                                                       ccGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTATGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCg                      <  1:923473/100‑1 (MQ=255)
                                                                        cGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCgg                     <  1:4296262/100‑1 (MQ=255)
                                                                        cGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCgg                     >  2:4193707/1‑100 (MQ=255)
                                                                          tGGGTCAATCGCAGTTCCGGCATGCCTGGCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGACGCTGCGCGGTTTCATTCTCTACGCCGGAc                   <  2:3754190/100‑1 (MQ=255)
                                                                          tGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGAc                   <  2:1983823/100‑1 (MQ=255)
                                                                          tGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGACGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGan                   <  1:4446762/100‑2 (MQ=255)
                                                                           gggTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACa                  >  2:5222164/1‑100 (MQ=255)
                                                                            ggTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGCCAg                 >  1:2063407/1‑100 (MQ=255)
                                                                               cAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTg              >  1:2952527/1‑100 (MQ=255)
                                                                                 aTCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGAc            >  1:3299171/1‑100 (MQ=255)
                                                                                   cGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACgg          <  2:1186107/100‑1 (MQ=255)
                                                                                   cGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACgg          >  2:1500650/1‑100 (MQ=255)
                                                                                    tctgTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGt         <  1:1422846/97‑1 (MQ=255)
                                                                                    gCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGt         >  1:5228317/1‑100 (MQ=255)
                                                                                     cAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTg        >  2:604027/1‑100 (MQ=255)
                                                                                       gTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGtaga      >  2:1404110/1‑97 (MQ=255)
                                                                                        ttCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCg     <  1:2167682/100‑1 (MQ=255)
                                                                                          ccGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTa   <  2:1864784/100‑1 (MQ=255)
                                                                                                 |                                                                                             
GCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAA  >  NC_000913/4291242‑4291432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: