Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,362,301 T→G pseudogene (53/84 nt) yjdQ ← pseudogene, P4‑like integrase remnant

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,362,3010TG99.6% 832.0 / NA 233pseudogene (53/84 nt)yjdQpseudogene, P4‑like integrase remnant
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base G (93/138);  minor base A (0/1);  total (93/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

GATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAA  >  NC_000913/4362205‑4362397
                                                                                                |                                                                                                
gATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTAt                                                                                               <  1:125519/100‑1 (MQ=255)
 aTTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATg                                                                                              <  2:2301026/100‑1 (MQ=255)
  ttGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATgg                                                                                             <  2:1723951/100‑1 (MQ=255)
  ttGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATgg                                                                                             >  1:1415174/1‑100 (MQ=255)
   tgtgAGTCCTTCCATGAGGAAGCTAACTGATACTTTTGTGTTTGTTCTCATGCCGAAAATATCATCGGTCGCCGTTCAGACCGGTTTTGTGCTGTATGGa                                                                                            <  2:4552985/100‑1 (MQ=255)
    gTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGAt                                                                                           <  1:5054518/100‑1 (MQ=255)
      tAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAACTCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGc                                                                                         <  2:4928614/100‑1 (MQ=255)
       aGTCTTTGCATGAGCAAGCGAACTGATACATTTCTTTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCg                                                                                        <  2:3301082/100‑1 (MQ=255)
       aGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCg                                                                                        <  2:4474324/100‑1 (MQ=255)
        gTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGt                                                                                       >  1:4690420/1‑100 (MQ=255)
        gTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGt                                                                                       <  2:134297/100‑1 (MQ=255)
         tCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGtt                                                                                      >  1:3228738/1‑100 (MQ=255)
         tCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGtt                                                                                      >  2:2819811/1‑100 (MQ=255)
          cTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTa                                                                                     >  1:430738/1‑100 (MQ=255)
          cTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTa                                                                                     >  2:2721791/1‑100 (MQ=255)
           tttGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTAc                                                                                    <  1:3864690/100‑1 (MQ=255)
           tttGCATGAGCAAGCGAACTGATACATTTCGCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTAc                                                                                    <  1:4373036/100‑1 (MQ=255)
             tGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGc                                                                                  <  1:943060/100‑1 (MQ=255)
             tGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGc                                                                                  <  2:41969/100‑1 (MQ=255)
             tGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGc                                                                                  <  1:4548267/100‑1 (MQ=255)
             tGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGc                                                                                  >  1:1975180/1‑100 (MQ=255)
              gCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGGTGTGTGGATGCGTTACGcc                                                                                 >  2:1099224/1‑99 (MQ=255)
               cATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTc                                                                                >  1:3739398/1‑100 (MQ=255)
                aTGAGCAAGCGCACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTAGTGCTGTATGGATGCGTTACGCTcc                                                                               <  2:4391392/100‑1 (MQ=255)
                 tGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATATCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCt                                                                              >  2:3217333/1‑100 (MQ=255)
                  gNGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCtg                                                                             >  2:1833575/1‑100 (MQ=255)
                  gAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCtg                                                                             <  2:3642083/100‑1 (MQ=255)
                   aGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCtga                                                                            >  1:568346/1‑100 (MQ=255)
                    gCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCtgat                                                                           >  1:5341327/1‑100 (MQ=255)
                     cAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCtgatg                                                                          <  1:3049604/100‑1 (MQ=255)
                     cAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCtgatg                                                                          <  1:3828920/100‑1 (MQ=255)
                     cAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCtgatg                                                                          <  1:22015/100‑1 (MQ=255)
                      aaGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCtgatga                                                                         <  1:2350348/100‑1 (MQ=255)
                       aGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGAc                                                                        >  2:4266880/1‑100 (MQ=255)
                          gAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTc                                                                     >  1:2390824/1‑100 (MQ=255)
                           aaCTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCa                                                                    >  1:3693613/1‑100 (MQ=255)
                              tGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCAttt                                                                 >  2:5023632/1‑100 (MQ=255)
                              tGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCAttt                                                                 <  1:1190933/100‑1 (MQ=255)
                              gatctcATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCAttt                                                                 <  1:1019157/95‑1 (MQ=255)
                               gATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTg                                                                <  2:5311402/100‑1 (MQ=255)
                                  aCATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACg                                                             <  1:90475/100‑1 (MQ=255)
                                  aCATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACg                                                             <  1:4147290/100‑1 (MQ=255)
                                   naTTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGt                                                            >  1:4437419/2‑100 (MQ=255)
                                   cATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGt                                                            <  2:3077751/100‑1 (MQ=255)
                                    aTTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGtt                                                           >  1:1657064/1‑100 (MQ=255)
                                     tttCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTc                                                          <  1:2989827/100‑1 (MQ=255)
                                     tttCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTc                                                          <  2:5458278/100‑1 (MQ=255)
                                        ctctTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAAc                                                       <  1:1436651/100‑1 (MQ=255)
                                         tctTTGTTCTCATTCAGAAAATCTCATCATTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACa                                                      <  1:5476260/100‑1 (MQ=255)
                                         tctTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACa                                                      <  1:356284/100‑1 (MQ=255)
                                         tctTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACa                                                      >  2:3437373/1‑100 (MQ=255)
                                         tctTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACa                                                      <  2:2432419/100‑1 (MQ=255)
                                           tttGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGc                                                    >  1:401975/1‑100 (MQ=255)
                                            ttGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCa                                                   <  2:2366830/100‑1 (MQ=255)
                                             tGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCAt                                                  <  1:4770614/100‑1 (MQ=255)
                                              gTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATc                                                 >  2:4169117/1‑100 (MQ=255)
                                               ttCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCa                                                >  2:4054871/1‑100 (MQ=255)
                                                     ttCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGc                                          >  1:3760373/1‑100 (MQ=255)
                                                     ttCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGc                                          <  2:1965625/100‑1 (MQ=255)
                                                       cAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCg                                        >  2:2843580/1‑100 (MQ=255)
                                                       cAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCg                                        >  2:1786092/1‑100 (MQ=255)
                                                        aGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGc                                       >  2:4352714/1‑100 (MQ=255)
                                                         gAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCCCGGGGCCGCa                                      >  1:334067/1‑100 (MQ=255)
                                                         gAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCa                                      <  2:4246092/100‑1 (MQ=255)
                                                          aaaaTCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCAc                                     <  2:612794/100‑1 (MQ=255)
                                                          aaaaTCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCAc                                     <  2:2684597/100‑1 (MQ=255)
                                                          aaaaTCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCAc                                     <  1:663979/100‑1 (MQ=255)
                                                           aaaTCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACg                                    <  2:5104822/100‑1 (MQ=255)
                                                             aTCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGan                                  <  2:3098378/100‑2 (MQ=255)
                                                             aTCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGAc                                  <  1:4174748/100‑1 (MQ=255)
                                                               ctcATCAGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACAt                                <  1:3586855/100‑1 (MQ=255)
                                                                 catcaGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACAttt                              >  2:5383914/1‑100 (MQ=255)
                                                                 catcaGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACAttt                              <  1:4794460/100‑1 (MQ=255)
                                                                  atcaGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTGCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTc                             <  1:203688/100‑1 (MQ=255)
                                                                   tcaGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCa                            <  2:2133997/100‑1 (MQ=255)
                                                                   tcaGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCa                            <  1:1500633/100‑1 (MQ=255)
                                                                   tcaGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCa                            <  2:5194225/100‑1 (MQ=255)
                                                                   tcaGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCa                            <  1:1806563/100‑1 (MQ=255)
                                                                   tcaGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCa                            >  1:2851385/1‑100 (MQ=255)
                                                                     aGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACg                          <  2:2053662/100‑1 (MQ=255)
                                                                     aGTCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACg                          <  1:518675/100‑1 (MQ=255)
                                                                       tCGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTc                        <  1:1284449/100‑1 (MQ=255)
                                                                        cGCCGTTCAGACCGGTGTTGTGCTGTATGGCTGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCa                       >  2:2906908/1‑100 (MQ=255)
                                                                        cGCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCa                       <  1:2561178/100‑1 (MQ=255)
                                                                         gCCGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAg                      <  1:3139470/100‑1 (MQ=255)
                                                                          ccGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGt                     <  1:3518404/100‑1 (MQ=255)
                                                                          ccGTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGt                     >  1:1734704/1‑100 (MQ=255)
                                                                            gTTCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTa                   >  1:3219907/1‑100 (MQ=255)
                                                                             ttCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAg                  <  1:2724676/100‑1 (MQ=255)
                                                                              tCAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGt                 <  1:2794249/100‑1 (MQ=255)
                                                                               cAGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTg                <  2:383368/100‑1 (MQ=255)
                                                                                aGACCGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGc               <  2:3396628/100‑1 (MQ=255)
                                                                                aGACCGGTCTTGTGCTGTATGGATGCGTTACGCTCCTGCTGACGTCATTTGACGTTCAACAGCATCACGGTGCCGCACGACATTTCACGTCAGTTAGTGc               <  1:5330033/100‑1 (MQ=255)
                                                                                   ccGGTGTTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGAGCCGCACGACATTTCACGTCAGTTAGTGCtat            <  1:762742/100‑1 (MQ=255)
                                                                                      ttgttgtGCCGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGc         <  2:4768255/99‑1 (MQ=255)
                                                                                      gtgtTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGc         <  1:5141594/100‑1 (MQ=255)
                                                                                      gtgtTGTGCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGc         <  2:1002146/100‑1 (MQ=255)
                                                                                           gtgCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAgg    <  2:2625464/100‑1 (MQ=255)
                                                                                             gCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGaa  >  2:1460308/1‑100 (MQ=255)
                                                                                             gCTGTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGaa  >  2:3741845/1‑100 (MQ=255)
                                                                                                |                                                                                                
GATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAA  >  NC_000913/4362205‑4362397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: