Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 546,173 T→G E64A (GAA→GCA)  allD ← ureidoglycolate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913546,1730TG99.1% 824.0 / ‑4.4 232E64A (GAA→GCA) allDureidoglycolate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (114/116);  total (115/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

TGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATA  >  NC_000913/546077‑546269
                                                                                                |                                                                                                
tGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTACAATTGCCGAGCACGGCCCTGTTTCCTCAAGACGAAACTCCGGTgcgc                                                                                               >  2:2355014/1‑100 (MQ=255)
 gTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGcgcg                                                                                              >  1:857669/1‑100 (MQ=255)
 gTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGcgcg                                                                                              >  1:1092138/1‑100 (MQ=255)
 gTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGcgcg                                                                                              >  1:3468101/1‑100 (MQ=255)
 gTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGcgcg                                                                                              >  1:2099406/1‑100 (MQ=255)
  ttCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCgg                                                                                             <  2:3206065/100‑1 (MQ=255)
   tCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGATTCCTCAAGACGAAACTCCGGTGCGCGGt                                                                                            >  2:5233940/1‑100 (MQ=255)
     cATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTg                                                                                          <  2:956824/100‑1 (MQ=255)
       tACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGt                                                                                        <  1:3502112/100‑1 (MQ=255)
         cccATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTgc                                                                                      >  1:126938/1‑100 (MQ=255)
         cccATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTgc                                                                                      >  2:5200491/1‑100 (MQ=255)
         cccATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTgc                                                                                      >  2:2207626/1‑100 (MQ=255)
         cccATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTgc                                                                                      >  2:2291267/1‑100 (MQ=255)
               ttCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCtt                                                                                >  1:5193810/1‑100 (MQ=255)
                tCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCttt                                                                               <  2:2433741/100‑1 (MQ=255)
                tCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCttt                                                                               >  1:4863088/1‑100 (MQ=255)
                  gccgcGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCAGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTg                                                                             >  2:900709/1‑100 (MQ=255)
                  gccgcGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACCGCCCGGCCTCCTCCAGACGACACTCCCGTGCGCGGGTGGTgccgcccggtg                                                                             >  2:4577299/1‑95 (MQ=255)
                    cgcgACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGaa                                                                           <  1:3704288/100‑1 (MQ=255)
                    cgcgACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGaa                                                                           <  1:2043418/100‑1 (MQ=255)
                      cgACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAAt                                                                         <  2:3797132/100‑1 (MQ=255)
                       gACCTGTCCGGCGGCATTGTAGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATg                                                                        >  1:4340975/1‑100 (MQ=255)
                        aCCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGc                                                                       <  1:417901/100‑1 (MQ=255)
                        aCCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGc                                                                       >  1:397982/1‑100 (MQ=255)
                          cTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGt                                                                     <  1:1919723/100‑1 (MQ=255)
                            gTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTc                                                                   >  1:350724/1‑100 (MQ=255)
                              ccGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCcg                                                                 >  1:1936672/1‑100 (MQ=255)
                                ggcggcATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCcgcg                                                               <  1:701803/100‑1 (MQ=255)
                                ggcggcATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCcgcg                                                               >  1:1571714/1‑100 (MQ=255)
                                   ggcATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGCGCCGCCTTTTGAAATGCGTTCCGCgtag                                                            <  1:245888/100‑1 (MQ=255)
                                   ggcATTGTCGGAAGGTAAAATTGGCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCGTTTGAAATGCGTTCCGCgtag                                                            <  2:4529891/100‑1 (MQ=255)
                                     cATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCgtagta                                                          >  1:640144/1‑100 (MQ=255)
                                     cATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCgtagta                                                          <  2:1578391/100‑1 (MQ=255)
                                     cATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGAGGTTGGTGCCGCCTTTTGAAATGCGCTACGCgtagta                                                          >  2:3397661/1‑100 (MQ=255)
                                      aTTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATTCGTTCCGCGTAGTAt                                                         >  2:2414053/1‑100 (MQ=255)
                                      aTTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTAt                                                         >  1:1928224/1‑100 (MQ=255)
                                         gTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTcc                                                      >  1:2844655/1‑100 (MQ=255)
                                          tCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCa                                                     <  2:2772309/100‑1 (MQ=255)
                                           cGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCAc                                                    <  1:3204178/100‑1 (MQ=255)
                                           cGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCAc                                                    >  1:2646949/1‑100 (MQ=255)
                                            ggCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCAcg                                                   >  1:4947954/1‑100 (MQ=255)
                                             gCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCAcgc                                                  >  1:4266234/1‑100 (MQ=255)
                                               aTGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAACTGCGTTCCGCGTAGTATTCCACgcgc                                                >  2:4386963/1‑100 (MQ=255)
                                               aTGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACgcgc                                                >  1:2392704/1‑100 (MQ=255)
                                               aTGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACgcgc                                                >  2:5073383/1‑100 (MQ=255)
                                               aTGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACgcgc                                                >  2:2097841/1‑100 (MQ=255)
                                                tGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCa                                               <  1:927438/100‑1 (MQ=255)
                                                tGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCa                                               <  2:3572615/100‑1 (MQ=255)
                                                tGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCa                                               >  1:4496186/1‑100 (MQ=255)
                                                tGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCa                                               <  1:3777635/100‑1 (MQ=255)
                                                tGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCa                                               >  1:5444909/1‑100 (MQ=255)
                                                 gTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCAc                                              >  1:2487199/1‑100 (MQ=255)
                                                   aaaaTTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACcg                                            >  2:1936759/1‑100 (MQ=255)
                                                      aTTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCgcgc                                         <  2:1290667/100‑1 (MQ=255)
                                                       ttGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGcc                                        <  1:3959384/100‑1 (MQ=255)
                                                       ttGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGcc                                        <  2:1752727/100‑1 (MQ=255)
                                                         gCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCAt                                      <  2:2808810/100‑1 (MQ=255)
                                                         gCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCAt                                      >  2:676908/1‑100 (MQ=255)
                                                          ccGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATg                                     <  2:1643657/100‑1 (MQ=255)
                                                          ccGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATg                                     <  2:4451876/100‑1 (MQ=255)
                                                          ccGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATg                                     <  1:5450748/100‑1 (MQ=255)
                                                           cGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATga                                    >  1:5342277/1‑100 (MQ=255)
                                                           cGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAAAGCGTTCCGCGTAGTATTCCACGCGCACCCCGCCATga                                    >  2:4997929/1‑100 (MQ=255)
                                                             aGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATgaga                                  <  1:2598892/100‑1 (MQ=255)
                                                               cACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGt                                <  1:3550110/100‑1 (MQ=255)
                                                                aCGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTg                               <  2:1907081/100‑1 (MQ=255)
                                                                aCGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTg                               <  2:268991/100‑1 (MQ=255)
                                                                aCGGCCCGGGTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTg                               <  2:4769246/100‑1 (MQ=255)
                                                                 cGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCAAGCGCACCGCGCCATGAGAGTgg                              <  1:695097/100‑1 (MQ=255)
                                                                  ggCCCGGTTTCCTCAGTACGAAACTCCGGTGCGCGGTTGGTGCCGACTTTTGAAATGCGTTCCGCGTAGTACTACACGCACACCGCGCCATGAGAGAGGa                             <  2:1298612/100‑1 (MQ=255)
                                                                   gCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGAAGTATTCAACGCGCACCGCGCCATGAGAGTGGAt                            >  2:2879850/1‑100 (MQ=255)
                                                                    cccGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATc                           <  2:5393333/100‑1 (MQ=255)
                                                                     ccGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGCTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATcc                          <  2:1380355/100‑1 (MQ=255)
                                                                      cGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAAGGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATccc                         <  2:721930/100‑1 (MQ=255)
                                                                           tCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTgg                    <  1:2686765/100‑1 (MQ=255)
                                                                           tCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTgg                    <  1:1725475/100‑1 (MQ=255)
                                                                            ccTCAAGACGAAACTCTGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGc                   <  2:3810246/100‑1 (MQ=255)
                                                                              tCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCAt                 >  2:2559186/1‑100 (MQ=255)
                                                                               cAAGACGAAACTCCGGTGTGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATc                <  1:3438109/100‑1 (MQ=255)
                                                                               cAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATc                <  2:4031940/100‑1 (MQ=255)
                                                                               cAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATc                >  2:1539553/1‑100 (MQ=255)
                                                                                aaGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCg               <  1:1477720/100‑1 (MQ=255)
                                                                                 aGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAACTGCGTTCCGCGTAGTATTCCACGCGCACCTCGCCATGGGCGTTCATCCCTCTGGCATCgg              <  2:4097068/100‑1 (MQ=255)
                                                                                  gACGAAACTCCGGTGTGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGc             >  1:5481443/1‑100 (MQ=255)
                                                                                   aCGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCg            <  1:2808136/100‑1 (MQ=255)
                                                                                   aCGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCg            >  1:957878/1‑100 (MQ=255)
                                                                                     gAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTa          >  1:5243291/1‑100 (MQ=255)
                                                                                     gAAACTCCAGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTa          >  2:5274814/1‑100 (MQ=255)
                                                                                       aaCTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTaaa        >  1:1184451/1‑100 (MQ=255)
                                                                                        aCTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAAc       <  2:4182885/100‑1 (MQ=255)
                                                                                        aCTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAAc       <  2:1683468/100‑1 (MQ=255)
                                                                                        aCTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAAc       <  2:1220324/100‑1 (MQ=255)
                                                                                         cTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAAcc      >  1:2156995/1‑100 (MQ=255)
                                                                                         cTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAAcc      <  2:5314846/100‑1 (MQ=255)
                                                                                         cTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAAcc      >  2:3117084/1‑100 (MQ=255)
                                                                                           ccGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCag    >  1:2872330/1‑99 (MQ=255)
                                                                                           ccGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCaa    >  1:3919019/1‑100 (MQ=255)
                                                                                           ccGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCaa    <  1:5047122/100‑1 (MQ=255)
                                                                                            cGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAAt   <  2:3484661/100‑1 (MQ=255)
                                                                                             ggTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATa  >  1:1842369/1‑100 (MQ=255)
                                                                                                |                                                                                                
TGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATA  >  NC_000913/546077‑546269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: