Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,576,712 T→G pseudogene (2794/2937 nt) yjiV → pseudogene; conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,576,7120TG97.3% 780.4 / 6.8 222pseudogene (2794/2937 nt)yjiVpseudogene; conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base G (106/110);  total (108/114)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 222 total aligned reads displayed.

TATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGTCGCCAACAGC  >  NC_000913/4576615‑4576807
                                                                                                 |                                                                                               
tatCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCa                                                                                               <  1:5354541/100‑1 (MQ=255)
 atCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCAt                                                                                              <  1:3374873/100‑1 (MQ=255)
   cccTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCg                                                                                            >  1:5131565/1‑100 (MQ=255)
    ccTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGc                                                                                           >  2:3979514/1‑100 (MQ=255)
    ccTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGc                                                                                           >  1:3209146/1‑100 (MQ=255)
    ccTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGc                                                                                           >  1:1623782/1‑100 (MQ=255)
     cTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCa                                                                                          >  2:2951189/1‑100 (MQ=255)
      tCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCaa                                                                                         <  2:50451/100‑1 (MQ=255)
       cACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAAt                                                                                        >  1:2992211/1‑100 (MQ=255)
        aCCAACGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTAGTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATg                                                                                       <  1:593621/100‑1 (MQ=255)
         ccATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATgg                                                                                      >  2:4239177/1‑100 (MQ=255)
         ccATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATgg                                                                                      >  2:2436684/1‑100 (MQ=255)
         ccATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGCATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATgg                                                                                      >  2:592635/1‑100 (MQ=255)
          cATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGc                                                                                     <  2:305868/100‑1 (MQ=255)
           aTCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCa                                                                                    >  1:4928017/1‑100 (MQ=255)
           aTCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCa                                                                                    <  2:625719/100‑1 (MQ=255)
            tCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCAt                                                                                   >  1:3495862/1‑100 (MQ=255)
             cGTAAAGTGACGGTGACTTTGGAGGATGGTCAGGTATTGAGGATCCGCTTCGATCAGGCGGGGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCAtt                                                                                  <  2:2111205/100‑1 (MQ=255)
             cGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCAtt                                                                                  >  1:2316397/1‑100 (MQ=255)
                 aaCTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACtt                                                                              <  1:4722082/100‑1 (MQ=255)
                   cTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTg                                                                            <  1:2534750/100‑1 (MQ=255)
                   cTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTg                                                                            <  1:499830/100‑1 (MQ=255)
                   cTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTg                                                                            <  2:2332721/100‑1 (MQ=255)
                    tGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGa                                                                           >  1:2720490/1‑100 (MQ=255)
                     gACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTTCATTGCAATGGCATTACTTTGAt                                                                          <  1:3788989/100‑1 (MQ=255)
                       cGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGAttt                                                                        >  1:811128/1‑100 (MQ=255)
                       cGGTGACTGTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGAttt                                                                        <  1:1425654/100‑1 (MQ=255)
                           gACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCcgc                                                                    <  2:3815303/100‑1 (MQ=255)
                             cTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGa                                                                  >  2:1827883/1‑100 (MQ=255)
                             cTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGa                                                                  >  1:426059/1‑100 (MQ=255)
                              ttttGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGAt                                                                 <  2:3559096/100‑1 (MQ=255)
                               tttGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGTATCGCAATGGCATTACTTTGATTTCCGCGATg                                                                <  2:177582/100‑1 (MQ=255)
                                ttGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGa                                                               <  2:1499759/100‑1 (MQ=255)
                                     gATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGttt                                                          <  2:2246861/100‑1 (MQ=255)
                                      aTGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGtttc                                                         <  1:1417679/100‑1 (MQ=255)
                                      aTGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGtttc                                                         <  2:4390803/100‑1 (MQ=255)
                                      aTGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGtttc                                                         <  1:5033412/100‑1 (MQ=255)
                                       tGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGtttct                                                        >  1:438711/1‑100 (MQ=255)
                                        tgTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGtttctt                                                       <  2:4725936/99‑1 (MQ=255)
                                        ggTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGtttctt                                                       <  2:1075982/100‑1 (MQ=255)
                                        cttatggTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGtttctt                                                       <  2:2888863/95‑1 (MQ=255)
                                         gTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGtttcttt                                                      >  1:2817071/1‑100 (MQ=255)
                                         gTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGtttctta                                                      >  1:4792781/1‑99 (MQ=255)
                                           cAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGCCGTTTCTTTcc                                                    <  2:5090598/100‑1 (MQ=255)
                                           cAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTcc                                                    <  2:162332/100‑1 (MQ=255)
                                           cAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTcc                                                    >  2:3396469/1‑100 (MQ=255)
                                           cAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTcc                                                    <  1:2427100/100‑1 (MQ=255)
                                           cAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTcc                                                    <  2:689784/100‑1 (MQ=255)
                                             ggTATTGAAGATCCGCTTCGATCAGGGTATGGGATACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAg                                                  <  2:489405/100‑1 (MQ=255)
                                             ggTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAg                                                  <  2:2423582/100‑1 (MQ=255)
                                             ggTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAg                                                  <  1:2217961/100‑1 (MQ=255)
                                              gTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGt                                                 >  2:4172733/1‑100 (MQ=255)
                                              gTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGt                                                 >  1:2579521/1‑100 (MQ=255)
                                              gTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGt                                                 <  1:1698422/100‑1 (MQ=255)
                                                aTTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTa                                               >  1:5404104/1‑100 (MQ=255)
                                                  tGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCCGTTAGt                                             >  2:525412/1‑100 (MQ=255)
                                                   gAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTACAGTTAGTc                                            >  2:4764900/1‑100 (MQ=255)
                                                     aGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCaa                                          >  2:2688522/1‑100 (MQ=255)
                                                      gATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCaaa                                         >  2:1949887/1‑100 (MQ=255)
                                                      gATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCaaa                                         >  1:1185182/1‑100 (MQ=255)
                                                      gATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCaaa                                         >  1:3743544/1‑100 (MQ=255)
                                                      gATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCaaa                                         >  2:1182020/1‑100 (MQ=255)
                                                        tCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAGAAt                                       <  1:4290050/100‑1 (MQ=255)
                                                        tCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAAt                                       >  1:2203625/1‑100 (MQ=255)
                                                        tCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAAt                                       <  1:5499625/100‑1 (MQ=255)
                                                           gCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGc                                    >  1:155340/1‑100 (MQ=255)
                                                              tCGATCAGGGGATGGGCTACTGGCGCAGCAACTTCGCATCGCAAGGGCATTACGTTCAGTTCCGCGATGACGTTTCTTCCCTGGGAGTTCAAATGGCTCa                                 <  2:1269956/100‑1 (MQ=255)
                                                               cGATCAGGGGATGGGCTACTGGCGCATGAACTTTGCATCGCAATGGCATTACTTTGATTGCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAg                                <  1:4822089/100‑1 (MQ=255)
                                                               cGATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAg                                <  2:4143575/100‑1 (MQ=255)
                                                                gATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAgg                               <  2:4484842/100‑1 (MQ=255)
                                                                gATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAgg                               <  1:3257146/100‑1 (MQ=255)
                                                                gATCAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAgg                               <  2:328223/100‑1 (MQ=255)
                                                                   cAGGGGATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCt                            >  2:2994897/1‑100 (MQ=255)
                                                                    aGGGGATGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTg                           >  2:1561370/1‑100 (MQ=255)
                                                                      gggATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCa                         >  1:3580621/1‑100 (MQ=255)
                                                                      gggATGGGCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCa                         <  2:677462/100‑1 (MQ=255)
                                                                          tGGGCTACTGGCGAATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCaagga                     >  1:5574640/1‑100 (MQ=255)
                                                                            ggCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCaaggaag                   >  1:5146696/1‑100 (MQ=255)
                                                                            ggCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCaaggaag                   >  1:641902/1‑100 (MQ=255)
                                                                            ggCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCaaggaag                   >  1:1321224/1‑100 (MQ=255)
                                                                            ggCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGAGTTCCGCGATGACGTTTCTTTCCAGTTCGTCAAAATGGCTCAGGCCGGCaaggaag                   <  2:3253790/100‑1 (MQ=255)
                                                                             gCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCaaggaagg                  <  2:4847565/100‑1 (MQ=255)
                                                                             gCTACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCaaggaagg                  <  2:1507562/100‑1 (MQ=255)
                                                                               tACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGa                <  1:850308/100‑1 (MQ=255)
                                                                               tACTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGa                >  2:5293752/1‑100 (MQ=255)
                                                                                aCTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGaa               >  2:1405489/1‑100 (MQ=255)
                                                                                 cTGGCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAAt              >  2:1097777/1‑100 (MQ=255)
                                                                                   ggCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTATTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGt            >  1:1963599/1‑100 (MQ=255)
                                                                                   ggCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGt            <  1:5443923/100‑1 (MQ=255)
                                                                                   ggCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGt            >  1:1660115/1‑100 (MQ=255)
                                                                                   ggCGCATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGt            >  1:161973/1‑100 (MQ=255)
                                                                                     cgcATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGTCg          >  1:4906506/1‑100 (MQ=255)
                                                                                     cgcAGCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCCTTCCAGTTAGGCAAAATGGCTCAGGCGTCCCAGGAAGGGAAtagcg          >  2:4676860/1‑96 (MQ=255)
                                                                                      gcATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGTCGc         >  2:5045913/1‑100 (MQ=255)
                                                                                       cATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGTCGcc        <  1:915715/100‑1 (MQ=255)
                                                                                       cATCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGTCGcc        >  1:3964273/1‑100 (MQ=255)
                                                                                        aTCAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGTCGCCa       <  1:4349609/100‑1 (MQ=255)
                                                                                          cAACTTTGCATCGCAATGGCATTACTTTGATTTCCTCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGTCGTCAAc     <  2:4644332/100‑1 (MQ=255)
                                                                                          cAACTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGTCGCCAAc     >  1:1043522/1‑100 (MQ=255)
                                                                                           aaCTTTGCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGTCGCCAACa    >  2:3473020/1‑100 (MQ=255)
                                                                                                 |                                                                                               
TATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCTGCAAGGAAGGGAATGTCGCCAACAGC  >  NC_000913/4576615‑4576807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: