Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 658,782 A→C pseudogene (558/594 nt) ybeM → pseudogene, C‑N hydrolase superfamily homology;putative enzyme; Not classified; putative amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913658,7820AC97.8% 779.8 / 4.7 225pseudogene (558/594 nt)ybeMpseudogene, C‑N hydrolase superfamily homology;putative enzyme; Not classified; putative amidase
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base C (127/92);  total (129/96)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

Only 100 of 226 total aligned reads displayed.

CGTCACCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTC  >  NC_000913/658686‑658877
                                                                                                |                                                                                               
   cACCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCaacaac                                                                                           >  1:1235294/1‑100 (MQ=255)
   cACCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCaacaac                                                                                           >  2:1875587/1‑100 (MQ=255)
   cACCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCaacaac                                                                                           >  1:5498735/1‑100 (MQ=255)
    aCCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAAcc                                                                                          <  1:1896559/100‑1 (MQ=255)
       aTTGCAGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTc                                                                                       >  2:2883729/1‑100 (MQ=255)
         tGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGc                                                                                     <  2:5410044/100‑1 (MQ=255)
          gCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCt                                                                                    <  2:5197780/100‑1 (MQ=255)
           cGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCtt                                                                                   >  2:134873/1‑100 (MQ=255)
             gCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTg                                                                                 >  1:1264667/1‑100 (MQ=255)
              cAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTgc                                                                                <  1:4974037/100‑1 (MQ=255)
               aGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTgcg                                                                               <  2:581351/100‑1 (MQ=255)
               aGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTgcg                                                                               >  2:2718284/1‑100 (MQ=255)
                  gTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccg                                                                            >  1:1470890/1‑100 (MQ=255)
                  gTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccg                                                                            >  1:65107/1‑100 (MQ=255)
                  gTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccg                                                                            >  1:5402907/1‑100 (MQ=255)
                   tCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccgc                                                                           >  1:3225553/1‑100 (MQ=255)
                   tCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccgc                                                                           >  1:3112216/1‑100 (MQ=255)
                    cAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccgcc                                                                          <  1:1830137/100‑1 (MQ=255)
                    cAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccgcc                                                                          >  2:2414001/1‑100 (MQ=255)
                    cAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccgcc                                                                          >  2:1809521/1‑100 (MQ=255)
                     aGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccgccg                                                                         <  1:4663503/100‑1 (MQ=255)
                     aGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccgccg                                                                         >  2:4669967/1‑100 (MQ=255)
                      gAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccgccgc                                                                        >  2:1946360/1‑100 (MQ=255)
                      gAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccgccgc                                                                        >  1:1062863/1‑100 (MQ=255)
                      gAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGcgccgccgc                                                                        >  1:2285858/1‑100 (MQ=255)
                        aaTGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCaa                                                                      >  1:3490131/1‑100 (MQ=255)
                         aTGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAat                                                                     <  2:3618170/100‑1 (MQ=255)
                           gCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAatta                                                                   >  2:336127/1‑100 (MQ=255)
                            ccTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAattat                                                                  >  2:444157/1‑100 (MQ=255)
                             cTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTGAACAACCGTCGCTTTGCGCCGCCGCAattatt                                                                 >  1:5097512/1‑100 (MQ=255)
                             cTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAattatt                                                                 >  2:3702396/1‑100 (MQ=255)
                              tGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAAttatta                                                                <  1:2911204/100‑1 (MQ=255)
                               gCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATtattat                                                               <  2:2631036/100‑1 (MQ=255)
                               gCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATtattat                                                               <  2:1239468/100‑1 (MQ=255)
                                  cTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTatgat                                                            >  1:2298064/1‑100 (MQ=255)
                                   tCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTatgatg                                                           <  2:4307010/100‑1 (MQ=255)
                                   tCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTatgatg                                                           <  1:339311/100‑1 (MQ=255)
                                   tCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTatgatg                                                           >  1:124183/1‑100 (MQ=255)
                                    cATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGt                                                          >  1:2122631/1‑100 (MQ=255)
                                     aTTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGtt                                                         >  1:1774509/1‑100 (MQ=255)
                                      ttgtGGCGGAAGTGCCGCCCGAACGCGTGCGTCAGTTGCCCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGttt                                                        <  2:2552632/97‑1 (MQ=255)
                                        ctGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCTACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGttttt                                                      <  2:3309783/99‑1 (MQ=255)
                                         tGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGtttttt                                                     <  1:1558401/100‑1 (MQ=255)
                                         tGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGtttttt                                                     <  2:1609104/100‑1 (MQ=255)
                                          ggCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGttttttt                                                    >  1:4882274/1‑100 (MQ=255)
                                          ggCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGtttttag                                                    >  1:3340870/1‑98 (MQ=255)
                                           gCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTa                                                   >  2:191867/1‑100 (MQ=255)
                                            cGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTAc                                                  >  1:201516/1‑100 (MQ=255)
                                              gAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCCATTATTATGATGTTTTTTTACTc                                                <  2:4031625/100‑1 (MQ=255)
                                              gAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTc                                                <  2:2528998/100‑1 (MQ=255)
                                               acctGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCg                                               >  2:2803153/4‑100 (MQ=255)
                                               acctGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCg                                               >  2:421908/4‑100 (MQ=255)
                                               aaGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCg                                               <  1:2476578/100‑1 (MQ=255)
                                                 gTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGgc                                             >  1:1507420/1‑100 (MQ=255)
                                                 gTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGgc                                             >  2:2116024/1‑100 (MQ=255)
                                                     cGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCtt                                         <  1:2603589/100‑1 (MQ=255)
                                                     cGCCCGAACGTGGGCGTCAGGTGCGCGCGGAACTGCCCGTCTTCAACAACCGTGGCTTTCCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCtt                                         <  2:2844352/100‑1 (MQ=255)
                                                      gCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTg                                        <  2:474490/100‑1 (MQ=255)
                                                      gCCCGAACGCGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGTGCTTg                                        <  2:4764990/100‑1 (MQ=255)
                                                       cccGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGa                                       >  2:2311995/1‑100 (MQ=255)
                                                       cccGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGa                                       >  1:4408012/1‑100 (MQ=255)
                                                       cccGAACGGGTGCGTCAGGTGCGCGCGCAACTGCCCGACTTCAACAACCGCCGCTTTGCGCCGCCGCACTTATTATGATGTTTTTTTACTCGGCGCTTgc                                       >  2:654225/1‑99 (MQ=255)
                                                        ccGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACATCCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGAt                                      <  2:1283038/100‑1 (MQ=255)
                                                         cGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGAtt                                     >  2:3177170/1‑100 (MQ=255)
                                                           aaCGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCa                                   <  2:4702909/100‑1 (MQ=255)
                                                            aCGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCAc                                  <  2:4568875/100‑1 (MQ=255)
                                                                gtgCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTg                              >  1:896796/1‑100 (MQ=255)
                                                                gtgCGTCAGGTGAGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTg                              >  1:4789476/1‑100 (MQ=255)
                                                                 tgCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGt                             <  1:2830955/100‑1 (MQ=255)
                                                                 tgCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGt                             >  1:1965115/1‑100 (MQ=255)
                                                                 tgCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGt                             <  1:4381723/100‑1 (MQ=255)
                                                                 tgCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGt                             <  1:426476/100‑1 (MQ=255)
                                                                 tgCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGCCCCTTTGCGCCGCAGCAATCATTATGCTGACTTTTTACTCGGCGCTTGATTCACCCTGt                             >  1:3284358/1‑100 (MQ=255)
                                                                  gCGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGtt                            <  2:4815258/100‑1 (MQ=255)
                                                                  gCGTCAGGTGCGCGCGCAACTGCCCGTCCTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGtt                            <  1:2658162/100‑1 (MQ=255)
                                                                   ggTCAGGTGGGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTTTTATGGTGTTTTTTTACTCGGCGCTTTGTTCACCTTGTTa                           <  2:4199352/99‑1 (MQ=255)
                                                                   cGTCAGGTGCGCGCGCACCTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTa                           <  2:3253838/100‑1 (MQ=255)
                                                                   cGTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTa                           <  2:4323573/100‑1 (MQ=255)
                                                                    gTCAGGTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTAc                          <  1:726862/100‑1 (MQ=255)
                                                                        ggTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGAt                      <  2:3040931/100‑1 (MQ=255)
                                                                        ggTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGAt                      <  1:4004869/100‑1 (MQ=255)
                                                                         gTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGAtt                     <  2:3812936/100‑1 (MQ=255)
                                                                         gTGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGAtt                     <  1:2969470/100‑1 (MQ=255)
                                                                          tGCGCGCGCAACTGCCCGTCTTCAACAACCGTCGCTTAGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTg                    >  2:5374826/1‑100 (MQ=255)
                                                                           gcgcgcgcAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGc                   <  2:5020869/100‑1 (MQ=255)
                                                                           gcgcgcgcAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGc                   <  1:2385687/100‑1 (MQ=255)
                                                                            cgcgcgcAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCt                  >  2:2985136/1‑100 (MQ=255)
                                                                             gcgcgcAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTa                 <  2:2584885/100‑1 (MQ=255)
                                                                             gcgcgcAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTa                 >  2:4903437/1‑100 (MQ=255)
                                                                                cgcAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATtg              <  1:3776956/100‑1 (MQ=255)
                                                                                 gcAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATtgt             <  2:1048794/100‑1 (MQ=255)
                                                                                  cAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTTTTCTGCGCTTGATTCACGTTGTTACAGATTGTTATtgtg            >  2:812029/1‑100 (MQ=255)
                                                                                  cAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATtgtg            >  2:3061435/1‑100 (MQ=255)
                                                                                  cAACTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATtgtg            >  1:3413424/1‑100 (MQ=255)
                                                                                   aaCTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATtgtgt           >  1:4044381/1‑100 (MQ=255)
                                                                                    aCTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATtgtgtg          <  2:2262483/100‑1 (MQ=255)
                                                                                    aCTGCCCGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATtgtgtg          >  1:282414/1‑100 (MQ=255)
                                                                                        cccGTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTgcgcg      >  1:1688989/1‑100 (MQ=255)
                                                                                           gTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGt   >  1:1812183/1‑100 (MQ=255)
                                                                                           gTCTTCAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGt   >  1:3878356/1‑100 (MQ=255)
                                                                                                |                                                                                               
CGTCACCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTC  >  NC_000913/658686‑658877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: