Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 711,781 T→G N40H (AAC→CAC)  ybfE ← LexA‑regulated protein, CopB family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913711,7810TG99.4% 627.7 / NA 179N40H (AAC→CAC) ybfELexA‑regulated protein, CopB family
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base G (89/88);  minor base A (1/0);  total (90/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

GCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACG  >  NC_000913/711685‑711877
                                                                                                |                                                                                                
   aCTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGttt                                                                                            >  1:4376194/1‑100 (MQ=255)
    cTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTtta                                                                                           <  1:4336321/100‑1 (MQ=255)
    cTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTAAGCTTCAGTTCGCTACCCTTAAGGCCCCGTACTTTGTCGCGTTTTAGCTTGTGGCGtatg                                                                                           >  2:2939111/1‑97 (MQ=255)
     tCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTttat                                                                                          >  2:3452236/1‑100 (MQ=255)
     tCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTttat                                                                                          <  1:4057397/100‑1 (MQ=255)
     tCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTttat                                                                                          <  2:3957031/100‑1 (MQ=255)
     tCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTttat                                                                                          <  1:1476769/100‑1 (MQ=255)
      ccGCCAGCTCGTTCAGCGCCTCGCCCGCTTCCGCGTTCAGCTTCAGTTCCACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTttatt                                                                                         <  2:4931366/100‑1 (MQ=255)
      ccGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTttatt                                                                                         >  2:3856653/1‑100 (MQ=255)
      ccGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTttatt                                                                                         >  2:65153/1‑100 (MQ=255)
      ccGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTttatt                                                                                         <  1:2020533/100‑1 (MQ=255)
      ccGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGCTTTAGCTGGTGGCGTttatt                                                                                         >  1:4396445/1‑100 (MQ=255)
       cGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTttatta                                                                                        <  2:834992/100‑1 (MQ=255)
       cGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTttatta                                                                                        <  1:1921175/100‑1 (MQ=255)
         ccAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAAt                                                                                      <  2:3049579/100‑1 (MQ=255)
          cAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATa                                                                                     <  2:2325516/100‑1 (MQ=255)
            gCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACg                                                                                   >  2:2675500/1‑100 (MQ=255)
             cTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGc                                                                                  <  1:805624/100‑1 (MQ=255)
              tCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCa                                                                                 <  2:3235119/100‑1 (MQ=255)
              tCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCa                                                                                 <  1:2869145/100‑1 (MQ=255)
               cGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAg                                                                                <  2:3280196/100‑1 (MQ=255)
                gTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGc                                                                               <  1:153377/100‑1 (MQ=255)
                gTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGc                                                                               <  1:1085932/100‑1 (MQ=255)
                gTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGGTTTAGCTGGTGCCGTTTATTAATACGCAGc                                                                               >  2:3103129/1‑100 (MQ=255)
                   cAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGt                                                                            >  2:4126398/1‑100 (MQ=255)
                    aGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGtt                                                                           >  1:4517094/1‑100 (MQ=255)
                    aGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGtt                                                                           <  2:2448691/100‑1 (MQ=255)
                      cgcCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCa                                                                         <  1:5527027/100‑1 (MQ=255)
                         cTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATcg                                                                      >  2:3808493/1‑100 (MQ=255)
                         cTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATcg                                                                      <  2:4846516/100‑1 (MQ=255)
                           cGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATcgcg                                                                    <  1:2226590/100‑1 (MQ=255)
                             aCAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATcgcgcg                                                                  <  2:1328950/100‑1 (MQ=255)
                             aCAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATcgcgcg                                                                  <  2:5043262/100‑1 (MQ=255)
                                gCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGaaa                                                               >  1:1061899/1‑100 (MQ=255)
                                      gcgTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGAt                                                         <  2:2081598/100‑1 (MQ=255)
                                       cgTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGAtt                                                        <  1:2774019/100‑1 (MQ=255)
                                       cgTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGAtt                                                        >  1:1722279/1‑100 (MQ=255)
                                        gTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTa                                                       >  1:3335524/1‑100 (MQ=255)
                                           cAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGtt                                                    <  1:1872088/100‑1 (MQ=255)
                                           cAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGtt                                                    <  1:1401368/100‑1 (MQ=255)
                                             gCTTCAGTTCGACACGCTTAAGACCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGtttt                                                  >  1:4656322/1‑100 (MQ=255)
                                              cTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTc                                                 <  1:2108243/100‑1 (MQ=255)
                                              cTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTc                                                 <  1:548597/100‑1 (MQ=255)
                                               ttCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCg                                                >  1:4711789/1‑100 (MQ=255)
                                               ttCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCg                                                >  1:3608578/1‑100 (MQ=255)
                                               ttCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTGTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCg                                                <  1:4918711/100‑1 (MQ=255)
                                                tCAGTTCGACACGCTTCAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCgg                                               <  1:4577782/100‑1 (MQ=255)
                                                tCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCgg                                               <  2:2654213/100‑1 (MQ=255)
                                                tCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCgg                                               <  2:3499448/100‑1 (MQ=255)
                                                tCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCgg                                               <  1:486446/100‑1 (MQ=255)
                                                tCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCgg                                               <  2:4467628/100‑1 (MQ=255)
                                                   gTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGgtcg                                            >  2:3317910/1‑100 (MQ=255)
                                                      cGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTcc                                         >  2:226520/1‑100 (MQ=255)
                                                      cGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTcc                                         <  1:2227987/100‑1 (MQ=255)
                                                       gACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTccn                                        <  2:2230302/100‑2 (MQ=255)
                                                       gACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTccc                                        <  2:4301466/100‑1 (MQ=255)
                                                       gACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTccc                                        <  2:156681/100‑1 (MQ=255)
                                                       gACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTccc                                        <  1:1837268/100‑1 (MQ=255)
                                                         cacGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCgg                                      <  2:1855891/100‑1 (MQ=255)
                                                         cacGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCgg                                      >  1:4794487/1‑100 (MQ=255)
                                                          acGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGt                                     <  1:3484589/100‑1 (MQ=255)
                                                          acGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGt                                     >  1:5235397/1‑100 (MQ=255)
                                                           cGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTc                                    <  1:4195676/100‑1 (MQ=255)
                                                           cGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTc                                    <  2:2728411/100‑1 (MQ=255)
                                                           cGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTc                                    <  2:3205121/100‑1 (MQ=255)
                                                           cGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTc                                    >  1:3261758/1‑100 (MQ=255)
                                                            gCTTAAGGCTACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCg                                   <  1:1125027/100‑1 (MQ=255)
                                                             cTTAAGGCCACGTACTTTGTCGCGTTCTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGa                                  <  2:3463756/100‑1 (MQ=255)
                                                               tAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACg                                <  2:359395/100‑1 (MQ=255)
                                                                  ggCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATGAGTTTTCGGTCGTCCCGGTCGACGCTc                             >  1:1652540/1‑100 (MQ=255)
                                                                   gCCACGTACTTTTGCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCGGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGATCg                            <  1:5018220/100‑1 (MQ=255)
                                                                   gCCACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTCTTCGGTCGTCCCGGTCGACGCTCg                            <  2:889122/100‑1 (MQ=255)
                                                                    ccACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGt                           >  1:49025/1‑100 (MQ=255)
                                                                     cACGTACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTg                          >  1:5260174/1‑100 (MQ=255)
                                                                         tACTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGcgcg                      >  1:3774480/1‑100 (MQ=255)
                                                                          aCTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGa                     >  2:5346001/1‑100 (MQ=255)
                                                                           cTTTGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGaa                    >  1:1070728/1‑100 (MQ=255)
                                                                            tttGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAAc                   >  1:898341/1‑100 (MQ=255)
                                                                             ttGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGCAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACa                  >  1:3337375/1‑100 (MQ=255)
                                                                              tGTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAg                 >  2:3566105/1‑100 (MQ=255)
                                                                               gTCGCGTTTTTGCTGGTGGCGTTTATTAATAGCTAGCTGTTCATCGCCTGAAAGCGGATTAGTTTTCGGTCGTGCCGGTTGACGCTCGCGCGCGCACCGa                >  2:558516/1‑100 (MQ=255)
                                                                               gTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGa                >  1:1318082/1‑100 (MQ=255)
                                                                               gTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGa                >  1:2090455/1‑100 (MQ=255)
                                                                               gTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGa                >  2:4079097/1‑100 (MQ=255)
                                                                               gTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGa                <  2:4988830/100‑1 (MQ=255)
                                                                               gTCGCGTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGa                >  1:4619201/1‑100 (MQ=255)
                                                                                 cgcgTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATc              >  2:2542496/1‑100 (MQ=255)
                                                                                 cgcgTGTTAGCTGGTGGCGGTTATTAATACGCAGCGGTTCATCGCGCGAAAGCGTATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATc              >  2:1631352/1‑100 (MQ=255)
                                                                                    gTTTTAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTaa           <  2:4399600/100‑1 (MQ=255)
                                                                                       ttAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGGTTAGTTTTCGGTCTCCCCTGTCGAGGCTCGTGCGCGAACAGATCTAATGt        <  2:283602/100‑1 (MQ=255)
                                                                                        tAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTc       >  2:4220602/1‑100 (MQ=255)
                                                                                        tAGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTc       <  1:2330356/100‑1 (MQ=255)
                                                                                         aGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCg      <  1:654964/100‑1 (MQ=255)
                                                                                         aGCTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCg      <  1:67528/100‑1 (MQ=255)
                                                                                           cTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTa    >  1:3812216/1‑100 (MQ=255)
                                                                                           cTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTa    <  1:3513371/100‑1 (MQ=255)
                                                                                           cTGGTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTa    >  1:688297/1‑100 (MQ=255)
                                                                                            tggtggCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTAc   <  1:415074/100‑1 (MQ=255)
                                                                                            tggtggCGTTTATTAAGACGCCGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTAc   <  2:413910/100‑1 (MQ=255)
                                                                                             ggTGGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGTGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACg  >  2:1700347/1‑100 (MQ=255)
                                                                                                |                                                                                                
GCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACG  >  NC_000913/711685‑711877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: