Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 783,757 A→C I198L (ATT→CTT)  pnuC → nicotinamide riboside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913783,7570AC99.2% 852.8 / ‑2.5 235I198L (ATT→CTT) pnuCnicotinamide riboside transporter
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (124/109);  total (125/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 235 total aligned reads displayed.

ATGATGGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCA  >  NC_000913/783661‑783852
                                                                                                |                                                                                               
   atgGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTg                                                                                           <  1:2229264/100‑1 (MQ=255)
    tgGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGc                                                                                          <  1:2347170/100‑1 (MQ=255)
      gtgtTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCAc                                                                                        >  1:4562904/1‑100 (MQ=255)
      gtgtTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCAc                                                                                        <  1:3137340/100‑1 (MQ=255)
       tgtTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACt                                                                                       >  1:582468/1‑100 (MQ=255)
       tgtTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGATGATTGTTAACGTGATTAGCGTCGTTCTTTTTGCACt                                                                                       >  2:4305310/1‑100 (MQ=255)
        gtTATCTATCGTGGCAATGATTCTGATGACGCGGAAGTAGGTGGAAAACTGGCTGTTGTGGGTGATGATTAACGTGATTATCGTCGTTCTTTTGGCACtg                                                                                      >  2:248915/1‑99 (MQ=255)
          tatctatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCa                                                                                    >  2:116349/1‑100 (MQ=255)
           atctatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAg                                                                                   >  2:2686544/1‑100 (MQ=255)
             ctatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAggg                                                                                 >  1:1257327/1‑100 (MQ=255)
             ctatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAggg                                                                                 >  2:1572306/1‑100 (MQ=255)
              tatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGc                                                                                >  1:5095760/1‑100 (MQ=255)
              tatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGc                                                                                >  1:3565170/1‑100 (MQ=255)
              tatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGc                                                                                >  1:2954002/1‑100 (MQ=255)
               atcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCg                                                                               >  2:4935219/1‑100 (MQ=255)
                  gTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGttt                                                                            <  2:1660245/100‑1 (MQ=255)
                  gTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGttt                                                                            >  1:190258/1‑100 (MQ=255)
                  gTGGCAATGATTCTGATGACGAGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGCTTATTAACGTCATTAGCGACGTTCTTTTTGCACTTCAAGGCCttt                                                                            >  2:3151293/1‑100 (MQ=255)
                    ggCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTAc                                                                          >  2:2529602/1‑100 (MQ=255)
                    ggCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTAc                                                                          <  1:4781743/100‑1 (MQ=255)
                    ggCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTAc                                                                          >  2:4139019/1‑100 (MQ=255)
                      cAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGc                                                                        >  2:5338641/1‑100 (MQ=255)
                       aaTGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGcc                                                                       >  1:2033729/1‑100 (MQ=255)
                       aaTGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGcc                                                                       >  1:4177147/1‑100 (MQ=255)
                       aaTGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGAGTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGcc                                                                       <  2:4866417/100‑1 (MQ=255)
                         tGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCAt                                                                     <  1:2365034/100‑1 (MQ=255)
                          gATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATg                                                                    >  2:4735523/1‑100 (MQ=255)
                          gATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATg                                                                    <  2:4342244/100‑1 (MQ=255)
                             tCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGtct                                                                 <  2:1874398/100‑1 (MQ=255)
                             tCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGtct                                                                 >  2:4209112/1‑100 (MQ=255)
                              cTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGtctc                                                                >  2:1744016/1‑100 (MQ=255)
                               tgatgaCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTctct                                                               <  1:1784009/100‑1 (MQ=255)
                                gatgaCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTg                                                              <  1:5004573/100‑1 (MQ=255)
                                  tgaCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGa                                                            >  1:1006209/1‑100 (MQ=255)
                                   gaCGCGTAAGTATGTGGAAAACTGGCTGTTGTGTGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAg                                                           <  2:1522046/100‑1 (MQ=255)
                                   gaCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAg                                                           <  2:2751847/100‑1 (MQ=255)
                                    aCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGt                                                          <  1:2595089/100‑1 (MQ=255)
                                     cgcgTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTa                                                         >  2:4081728/1‑100 (MQ=255)
                                     cgcgTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGCTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTa                                                         >  2:3243819/1‑100 (MQ=255)
                                      gcgTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTAc                                                        <  2:1017795/100‑1 (MQ=255)
                                       cgTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTAca                                                       >  2:1088024/1‑100 (MQ=255)
                                         tAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGTCATGTCTCTGGAGTAcatc                                                     <  1:1761652/100‑1 (MQ=255)
                                          aaGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTAcatca                                                    <  2:3786555/100‑1 (MQ=255)
                                            gTATGTGGAAAACTGGCTGTTGTGGGGGATTAGTAACGTGATTAGCGTCGTTCGGTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACatcatc                                                  >  2:5225516/1‑100 (MQ=255)
                                             tATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATcc                                                 >  2:4432769/1‑100 (MQ=255)
                                             tATGGGGAAAACGGGGTGGGGGGGGTGATGGGTAACGTGAGTAGCGGCGTTCGTTTTGCTCTTCAGGGCGGTTTCGCCGGGTCTCTGGAGTACATCATcc                                                 <  2:2301132/100‑1 (MQ=255)
                                              aTGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCt                                                >  1:3509391/1‑100 (MQ=255)
                                               tgtgGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTg                                               >  1:3809166/1‑100 (MQ=255)
                                                 tgGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGAc                                             <  2:5128865/100‑1 (MQ=255)
                                                 tgGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGAc                                             <  2:3641772/100‑1 (MQ=255)
                                                    aaaaCTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCtt                                          <  1:869174/100‑1 (MQ=255)
                                                    aaaaCTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCtt                                          <  1:869672/100‑1 (MQ=255)
                                                     aaaCTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCttt                                         >  2:4574921/1‑100 (MQ=255)
                                                     aaaCTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCttt                                         <  1:440407/100‑1 (MQ=255)
                                                       aCTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTAt                                       <  2:2926220/100‑1 (MQ=255)
                                                          ggCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTgc                                    >  1:3852219/1‑100 (MQ=255)
                                                          ggCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTgc                                    >  1:2017747/1‑100 (MQ=255)
                                                            cTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTgcgc                                  <  2:1716623/100‑1 (MQ=255)
                                                             tgttgtGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCt                                 <  1:5411483/100‑1 (MQ=255)
                                                             tgttgtGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCt                                 >  2:3570517/1‑100 (MQ=255)
                                                                 gtgGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAAc                             <  2:3268609/100‑1 (MQ=255)
                                                                 gtgGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAAc                             <  2:728563/100‑1 (MQ=255)
                                                                 gtgGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAAc                             >  1:3954250/1‑100 (MQ=255)
                                                                  tgGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACg                            >  2:1138228/1‑100 (MQ=255)
                                                                   gggTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGCACATCATCCTGACCTTTATTGCGCTCAACgg                           >  1:5233321/1‑100 (MQ=255)
                                                                    ggTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGc                          >  1:98765/1‑100 (MQ=255)
                                                                    ggTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGc                          <  2:4621743/100‑1 (MQ=255)
                                                                    ggTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGc                          >  2:304409/1‑100 (MQ=255)
                                                                     gTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCa                         <  1:5020330/100‑1 (MQ=255)
                                                                     gTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCa                         >  2:670418/1‑100 (MQ=255)
                                                                      tGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAg                        >  2:1378299/1‑100 (MQ=255)
                                                                      tGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAg                        <  1:1821639/100‑1 (MQ=255)
                                                                       gATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGc                       >  1:4060249/1‑100 (MQ=255)
                                                                        attattAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGcc                      <  1:5153773/100‑1 (MQ=255)
                                                                         ttattaACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCg                     >  2:336563/1‑100 (MQ=255)
                                                                         ttattaACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCg                     >  1:3420641/1‑100 (MQ=255)
                                                                           attaACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGa                   >  1:5318118/1‑100 (MQ=255)
                                                                             taACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGAtg                 <  1:1520193/100‑1 (MQ=255)
                                                                             taACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGAtg                 <  2:4128512/100‑1 (MQ=255)
                                                                              aaCGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGCGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGCtgt                >  2:4820405/1‑100 (MQ=255)
                                                                                  tGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGag            >  1:3593684/1‑99 (MQ=255)
                                                                                  tGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGag            >  1:4967870/1‑99 (MQ=255)
                                                                                  tGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGAt            >  1:1076641/1‑100 (MQ=255)
                                                                                  tGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGAt            <  1:3682074/100‑1 (MQ=255)
                                                                                  tGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGAt            <  1:4475497/100‑1 (MQ=255)
                                                                                  tGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGAt            <  2:3508558/100‑1 (MQ=255)
                                                                                   gATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATc           >  2:448576/1‑100 (MQ=255)
                                                                                   gATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATc           <  1:4493713/100‑1 (MQ=255)
                                                                                   gATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATc           <  1:5514478/100‑1 (MQ=255)
                                                                                   gATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATc           <  2:715205/100‑1 (MQ=255)
                                                                                    aTTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCa          <  1:2605472/100‑1 (MQ=255)
                                                                                     ttAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCaa         >  1:2800254/1‑100 (MQ=255)
                                                                                      tAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc        >  1:4334863/1‑100 (MQ=255)
                                                                                       aGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACa       >  1:2274904/1‑100 (MQ=255)
                                                                                        gCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAg      <  1:3298216/100‑1 (MQ=255)
                                                                                        gCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAg      <  1:2502908/100‑1 (MQ=255)
                                                                                        gCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAg      >  1:5534175/1‑100 (MQ=255)
                                                                                        gCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAg      >  1:764667/1‑100 (MQ=255)
                                                                                          gtcgtTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAgcg    >  1:1198131/1‑100 (MQ=255)
                                                                                            cgtTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCa  <  2:5479522/100‑1 (MQ=255)
                                                                                                |                                                                                               
ATGATGGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCA  >  NC_000913/783661‑783852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: