Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 960,657 C→T L132L (CTG→TTG)  ycaL → putative peptidase‑related chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913960,6570CT99.5% 620.3 / NA 183L132L (CTG→TTG) ycaLputative peptidase‑related chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (97/85);  minor base G (0/1);  total (97/86)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

GACCAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTCTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCAGCTA  >  NC_000913/960560‑960753
                                                                                                 |                                                                                                
gACCAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTg                                                                                                <  1:1072820/100‑1 (MQ=255)
gACCAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTg                                                                                                <  2:332676/100‑1 (MQ=255)
  ccAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTggg                                                                                              <  2:2025015/100‑1 (MQ=255)
  ccAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTggg                                                                                              >  1:1939806/1‑100 (MQ=255)
  ccAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGGGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTggg                                                                                              >  2:248948/1‑100 (MQ=255)
  caaGCGAGGTTCACGCATGGGCGATTGAGCACGTGTGTGTGCGTGTCTACAGGCGACTGATGGACATGATGAATGACAAGGAAATTGTAGGCGATGTggg                                                                                              <  2:5374957/98‑1 (MQ=255)
   caaacaCGTCAAAGCATGGGCGTTGTCAAACGGCTGTGTTCGTGTCTACAGTGGCCGGATGGACAAGATCAATGGCAACGAATTTGAAGGCGGTTTGGGc                                                                                             >  2:1039117/6‑100 (MQ=255)
   cAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGc                                                                                             <  1:2235736/100‑1 (MQ=255)
   cAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGc                                                                                             >  1:5353563/1‑100 (MQ=255)
   cAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGc                                                                                             >  1:375147/1‑100 (MQ=255)
    aGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGcc                                                                                            >  2:570224/1‑100 (MQ=255)
        aCGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGa                                                                                        <  2:2051423/100‑1 (MQ=255)
         cGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGaa                                                                                       >  2:2854321/1‑100 (MQ=255)
          gTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAAc                                                                                      >  1:855709/1‑100 (MQ=255)
             aaCGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTgg                                                                                   <  2:2431545/100‑1 (MQ=255)
              aCGCATGGGCGTTGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAAGTGAAGGCGTTTTGCGCCATGAACTggg                                                                                  <  1:4916043/100‑1 (MQ=255)
              aCGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTggg                                                                                  <  1:5568881/100‑1 (MQ=255)
              aCGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTggg                                                                                  <  1:2481228/100‑1 (MQ=255)
               cGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGc                                                                                 >  1:1603993/1‑100 (MQ=255)
               cGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGc                                                                                 >  2:1930045/1‑100 (MQ=255)
                gCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGcc                                                                                >  2:2362534/1‑100 (MQ=255)
                gCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGcc                                                                                <  2:3513344/100‑1 (MQ=255)
                  aTGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCAc                                                                              >  2:282363/1‑100 (MQ=255)
                   tGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACg                                                                             >  1:268968/1‑100 (MQ=255)
                     ggCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTc                                                                           >  1:3499720/1‑100 (MQ=255)
                       cGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGCATTGGGACCTGACCTGGGCCCCGTagc                                                                         >  2:2753852/1‑100 (MQ=255)
                          tGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGtt                                                                      >  1:3872014/1‑100 (MQ=255)
                            gCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTgg                                                                    >  1:3467863/1‑100 (MQ=255)
                            gCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTgg                                                                    <  2:2579781/100‑1 (MQ=255)
                             cGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTggg                                                                   >  2:853851/1‑100 (MQ=255)
                             cGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTggg                                                                   <  1:1105403/100‑1 (MQ=255)
                             cGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTggg                                                                   <  1:1422659/100‑1 (MQ=255)
                             cGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTggg                                                                   <  1:2772888/100‑1 (MQ=255)
                             cGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTggg                                                                   <  1:2401303/100‑1 (MQ=255)
                             cGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATAGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGACGCGTTggg                                                                   >  2:1205894/1‑100 (MQ=255)
                              gAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTgggn                                                                  <  1:4435657/100‑2 (MQ=255)
                               aaCGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTc                                                                 <  2:5042765/100‑1 (MQ=255)
                                 cGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCAc                                                               <  1:287283/100‑1 (MQ=255)
                                   gCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTc                                                             >  2:446881/1‑100 (MQ=255)
                                       tgtTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCgctg                                                         <  2:1717409/100‑1 (MQ=255)
                                        gtTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCgctgg                                                        <  2:163474/100‑1 (MQ=255)
                                          tCGTGTCTACAGTGGCCGGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGCCGCGTTGGGTCACTCgctggct                                                      >  2:5260940/1‑100 (MQ=255)
                                           cGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCgctggctg                                                     >  2:3796996/1‑100 (MQ=255)
                                           cGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCgctggctg                                                     <  2:1797252/100‑1 (MQ=255)
                                             tgtCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCtgaa                                                   >  1:4407055/1‑100 (MQ=255)
                                             tgtCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCtgaa                                                   <  1:3488674/100‑1 (MQ=255)
                                              gtCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCtgaaa                                                  >  2:2912152/1‑100 (MQ=255)
                                               tCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCtgaaat                                                 <  1:4905025/100‑1 (MQ=255)
                                                    aGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAg                                            <  1:285070/100‑1 (MQ=255)
                                                     gTGGCCTGATTGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGc                                           <  1:2039517/100‑1 (MQ=255)
                                                      tGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGct                                          <  1:889992/100‑1 (MQ=255)
                                                       ggCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGctt                                         >  1:3029866/1‑100 (MQ=255)
                                                       ggCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGctt                                         >  1:4378869/1‑100 (MQ=255)
                                                       ggCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGctt                                         <  1:4402899/100‑1 (MQ=255)
                                                        gCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGCACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGcttc                                        >  2:4981851/1‑100 (MQ=255)
                                                         ccTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGcttct                                       <  1:3870155/100‑1 (MQ=255)
                                                         ccTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGcttct                                       <  2:665183/100‑1 (MQ=255)
                                                          cTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGcttctt                                      >  2:1186554/1‑100 (MQ=255)
                                                           tGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTa                                     >  1:871542/1‑100 (MQ=255)
                                                            gATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTAt                                    >  2:4283127/1‑100 (MQ=255)
                                                            gATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTAt                                    <  2:4890415/100‑1 (MQ=255)
                                                             aTGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATg                                   <  1:1812901/100‑1 (MQ=255)
                                                             aTGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATg                                   <  1:1281314/100‑1 (MQ=255)
                                                             aTGGACATGATGAATGACAACGAAACTGAAGGCGTTTTGGGCCCTGAACTTGGCCACGTCTCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATg                                   <  2:791225/100‑1 (MQ=255)
                                                              tGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGc                                  <  1:4322913/100‑1 (MQ=255)
                                                               ggACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCTACGTCGCGTTGGGTCACTCGCCGGCTGAAATGAAAGCTTCTTATGCg                                 <  1:4980192/100‑1 (MQ=255)
                                                                 aCATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGAt                               >  2:3269260/1‑100 (MQ=255)
                                                                 aCATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGAt                               >  1:4630112/1‑100 (MQ=255)
                                                                    tgatgaCTGACAACGAAACTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGt                            >  1:4636361/1‑100 (MQ=255)
                                                                    tgatgaATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGt                            >  2:4114479/1‑100 (MQ=255)
                                                                    tgatgaATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGt                            >  1:4221831/1‑100 (MQ=255)
                                                                    tgatgaATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGt                            >  2:1544167/1‑100 (MQ=255)
                                                                     gatgaATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGCAAGCTTCTTATGCGATCGtt                           >  2:4011411/1‑100 (MQ=255)
                                                                      atgaatgaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTg                          <  1:1734068/100‑1 (MQ=255)
                                                                       tgaatgaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGc                         >  2:5205914/1‑100 (MQ=255)
                                                                          atgaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGc                      >  1:65796/1‑100 (MQ=255)
                                                                           tgaCAACGAAATTGAAGGCGTTTTGGGCCAATAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTTCCGCa                     <  2:1205106/100‑1 (MQ=255)
                                                                             aCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCAcg                   >  2:4797480/1‑100 (MQ=255)
                                                                              cAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATTCGATCGTTGCCGCAcgc                  >  2:1311648/1‑100 (MQ=255)
                                                                               aaCGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCAcgcg                 <  1:4826497/100‑1 (MQ=255)
                                                                                aCGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGa                >  1:4353894/1‑100 (MQ=255)
                                                                                aCGAAATTGAAGGCGGTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGa                >  2:1783049/1‑100 (MQ=255)
                                                                                 cGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGAt               >  2:2648634/1‑100 (MQ=255)
                                                                                 cGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGAt               >  1:2281054/1‑100 (MQ=255)
                                                                                 cGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGAt               <  2:4372947/100‑1 (MQ=255)
                                                                                  gAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATg              >  1:315801/1‑100 (MQ=255)
                                                                                  gAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATg              <  1:266650/100‑1 (MQ=255)
                                                                                   aaaTTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGc             >  2:2163715/1‑100 (MQ=255)
                                                                                     aTTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCa           <  2:2397534/100‑1 (MQ=255)
                                                                                       tGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCAtt         <  1:4617036/100‑1 (MQ=255)
                                                                                       tGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCAtt         >  2:5444578/1‑100 (MQ=255)
                                                                                       tGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCAtt         >  1:2887172/1‑100 (MQ=255)
                                                                                       tGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCAtt         >  2:3194420/1‑100 (MQ=255)
                                                                                       tGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCAtt         >  1:4760973/1‑100 (MQ=255)
                                                                                          aGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCa      <  2:4130282/100‑1 (MQ=255)
                                                                                           ggCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCAg     >  2:4234539/1‑100 (MQ=255)
                                                                                            gCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCAGc    <  2:2764629/100‑1 (MQ=255)
                                                                                             cGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCAGCt   >  2:281440/1‑100 (MQ=255)
                                                                                              gTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCAGCTa  >  1:487111/1‑100 (MQ=255)
                                                                                              gTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCAGCTa  >  1:2713167/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GACCAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTCTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCAGCTA  >  NC_000913/960560‑960753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: