Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,048,134 A→C N186K (AAT→AAG gfcC ← putative O‑antigen capsule production periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,048,1340AC97.2% 724.9 / 4.5 213N186K (AAT→AAGgfcCputative O‑antigen capsule production periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base C (108/97);  total (112/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 219 total aligned reads displayed.

AAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCA  >  NC_000913/1048037‑1048230
                                                                                                 |                                                                                                
 aaGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGttcttc                                                                                               <  1:1258638/100‑1 (MQ=255)
  aGCTGGCTGCCTGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTtcttct                                                                                              <  1:4911124/100‑1 (MQ=255)
  aGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTtcttct                                                                                              <  1:4987675/100‑1 (MQ=255)
  aGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTtcttct                                                                                              <  2:406737/100‑1 (MQ=255)
       gctgCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTcc                                                                                         <  1:4237506/100‑1 (MQ=255)
       gctgCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTcc                                                                                         >  2:4520441/1‑100 (MQ=255)
         tgCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCcgc                                                                                       <  1:2513653/100‑1 (MQ=255)
         tgCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCcgc                                                                                       >  2:2401445/1‑100 (MQ=255)
          gCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCcgcg                                                                                      >  2:4521429/1‑100 (MQ=255)
          gCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCcgcg                                                                                      >  2:5190307/1‑100 (MQ=255)
            cAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGcc                                                                                    >  1:3786899/1‑100 (MQ=255)
            cAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGcc                                                                                    >  1:3344334/1‑100 (MQ=255)
            cAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGcc                                                                                    <  2:2243696/100‑1 (MQ=255)
            cAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGcc                                                                                    >  1:2187762/1‑100 (MQ=255)
             aGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGTCGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTGCGCGCCt                                                                                   >  2:5112960/1‑100 (MQ=255)
             aGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCt                                                                                   <  1:1935952/100‑1 (MQ=255)
                cGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCa                                                                                <  2:398184/100‑1 (MQ=255)
                 ggtggtTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCaa                                                                               >  1:4606358/1‑100 (MQ=255)
                 ggtggtTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCaa                                                                               >  2:1053062/1‑100 (MQ=255)
                   tggtTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAgg                                                                             <  2:4493470/100‑1 (MQ=255)
                       tCCACATGTCTTTTATTCCACAGTGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggg                                                                         >  2:1144795/1‑100 (MQ=255)
                       tCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggg                                                                         <  2:3237390/100‑1 (MQ=255)
                       tCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggg                                                                         <  2:514210/100‑1 (MQ=255)
                       tCCACATAACTTTTATTCCACAGGACAACACGCGCCACGACAGTCACACCCTCATCCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggt                                                                         <  2:1320374/100‑2 (MQ=255)
                         cacaTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggggg                                                                       <  1:200229/100‑1 (MQ=255)
                         cacaTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggggg                                                                       <  2:2102244/100‑1 (MQ=255)
                         cacaTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggggg                                                                       <  2:1364354/100‑1 (MQ=255)
                         cacaTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggggg                                                                       >  2:4596437/1‑100 (MQ=255)
                         cacaTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggggg                                                                       <  1:4374548/100‑1 (MQ=255)
                         cacaTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggggg                                                                       <  2:595286/100‑1 (MQ=255)
                         cacaTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggggg                                                                       >  1:5206287/1‑100 (MQ=255)
                           caTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTg                                                                     <  2:1009671/100‑1 (MQ=255)
                           caTGTCTTTTAGTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTCATCAACGTAAAGTTCTTCTTGTCCGCGACTGCATGGCgggtctg                                                                     >  2:2580872/1‑96 (MQ=255)
                             tGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGAt                                                                   >  2:3436437/1‑100 (MQ=255)
                              gTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATc                                                                  <  2:3367112/100‑1 (MQ=255)
                                cTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCtt                                                                >  1:654524/1‑100 (MQ=255)
                                cTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCtt                                                                >  2:919862/1‑100 (MQ=255)
                                cTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCtt                                                                >  1:2412373/1‑100 (MQ=255)
                                cTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCtt                                                                >  1:1532735/1‑100 (MQ=255)
                                 ttttATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTg                                                               >  2:1944942/1‑100 (MQ=255)
                                  tttATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGc                                                              <  2:3921002/100‑1 (MQ=255)
                                   ttATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCa                                                             >  1:5160698/1‑100 (MQ=255)
                                   ttATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCa                                                             <  2:3498385/100‑1 (MQ=255)
                                    tATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCan                                                            <  1:4443835/100‑2 (MQ=255)
                                     aTTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGCGATCTTGCAGa                                                           >  2:4393003/1‑100 (MQ=255)
                                      ttCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGAt                                                          <  1:4879251/100‑1 (MQ=255)
                                        ccACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGNCTGCAAGACGGGGNTGATCTTGCAGATaa                                                        >  2:2448151/1‑100 (MQ=255)
                                        ccACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATaa                                                        >  1:2598347/1‑100 (MQ=255)
                                        ccACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATaa                                                        >  1:875938/1‑100 (MQ=255)
                                        ccACAAGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATaa                                                        >  2:4836674/1‑100 (MQ=255)
                                          acaGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATc                                                      >  2:3609849/1‑100 (MQ=255)
                                             gggCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGt                                                   >  2:2940376/1‑100 (MQ=255)
                                             gggCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGt                                                   <  1:1740527/100‑1 (MQ=255)
                                             gggCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTACTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGt                                                   >  2:4981443/1‑100 (MQ=255)
                                              ggCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTa                                                  <  1:3037028/100‑1 (MQ=255)
                                                   cAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCt                                             >  2:5364328/1‑100 (MQ=255)
                                                   cAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCt                                             >  1:5233433/1‑100 (MQ=255)
                                                     ggggCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTAc                                           >  1:3227882/1‑100 (MQ=255)
                                                      gggCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACg                                          <  1:3538029/100‑1 (MQ=255)
                                                      gggCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACg                                          >  2:4215634/1‑100 (MQ=255)
                                                         cgacgaCAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcc                                       <  2:4240139/100‑1 (MQ=255)
                                                            cgacAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctgc                                    >  1:1860251/1‑100 (MQ=255)
                                                             gacAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctgcc                                   >  1:3964615/1‑100 (MQ=255)
                                                             gacAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctgcc                                   <  1:5084832/100‑1 (MQ=255)
                                                             gacAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctgcc                                   <  1:803156/100‑1 (MQ=255)
                                                               cAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGcctgcctg                                 <  2:2175488/100‑1 (MQ=255)
                                                               cAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGcctgcctg                                 <  1:1442997/100‑1 (MQ=255)
                                                               cAGTCTCACCCACAGTCGTAAACACCGTAACAGCGGTCTGATCCGCGCCTGCAAGGCTGGCGGGAGCAGGCAGGTAATCGCTAACGCTACGGcctgcctg                                 >  2:4154368/1‑100 (MQ=255)
                                                                aGTCTCTCCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCctgcctgc                                <  2:433814/100‑1 (MQ=255)
                                                                   ctcACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAg                             >  1:1610198/1‑100 (MQ=255)
                                                                   ctcACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAg                             <  1:4234914/100‑1 (MQ=255)
                                                                     cACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAggg                           >  1:2966546/1‑100 (MQ=255)
                                                                      aCCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGc                          <  1:2197571/100‑1 (MQ=255)
                                                                      aCCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGc                          <  1:2819578/100‑1 (MQ=255)
                                                                      aCCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGc                          <  2:1284069/100‑1 (MQ=255)
                                                                       cccTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCa                         >  2:304805/1‑100 (MQ=255)
                                                                       cccTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCa                         >  2:3711082/1‑100 (MQ=255)
                                                                         cTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAAt                       >  2:4803919/1‑100 (MQ=255)
                                                                         cTCAGGCGTAATCACCATAACGTTCTTCTTGGCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAAt                       <  2:2505304/100‑1 (MQ=255)
                                                                          tCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAAtt                      <  1:2874482/100‑1 (MQ=255)
                                                                           cAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTg                     >  2:2838613/1‑100 (MQ=255)
                                                                           cAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTg                     >  2:1541667/1‑100 (MQ=255)
                                                                            aGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGc                    >  2:3596907/1‑100 (MQ=255)
                                                                             ggCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCCGGGCAATTGcc                   <  2:2984680/100‑1 (MQ=255)
                                                                             ggCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGcc                   >  1:1057335/1‑100 (MQ=255)
                                                                             ggCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGcc                   >  2:2572068/1‑100 (MQ=255)
                                                                             ggCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGcc                   >  2:1916939/1‑100 (MQ=255)
                                                                              gCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGCTAACGCTACGGCCTGCCTGCCAGGGCAATTGccc                  <  2:5084845/100‑1 (MQ=255)
                                                                               cGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCg                 >  2:1327663/1‑100 (MQ=255)
                                                                                   aTCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGccc             <  1:965944/100‑1 (MQ=255)
                                                                                    tCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGcccc            >  1:3651246/1‑100 (MQ=255)
                                                                                     cACCATAACGTTCTTCTTGTCCGCTCCTTCAAGGCGGTGTTGATCTTGCTGTTTATCTGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCa           >  2:2554319/1‑100 (MQ=255)
                                                                                      aCCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAg          <  1:1309851/100‑1 (MQ=255)
                                                                                         aTAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGaaa       <  2:3144594/100‑1 (MQ=255)
                                                                                         aTAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGaaa       >  1:4540255/1‑100 (MQ=255)
                                                                                          tAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAAc      <  2:4460518/100‑1 (MQ=255)
                                                                                            aCGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCg    <  2:3257978/100‑1 (MQ=255)
                                                                                            aCGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCg    >  1:1801668/1‑100 (MQ=255)
                                                                                            aCGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCg    <  1:3272723/100‑1 (MQ=255)
                                                                                              gTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCa  >  1:4546883/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCA  >  NC_000913/1048037‑1048230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: