Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,139,916 T→G D513E (GAT→GAG flgK → flagellar hook‑filament junction protein 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,139,9160TG98.8% 600.1 / ‑2.2 169D513E (GAT→GAGflgKflagellar hook‑filament junction protein 1
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (81/86);  total (83/86)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 179 total aligned reads displayed.

ATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAAC  >  NC_000913/1139819‑1140012
                                                                                                 |                                                                                                
 taaAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCgaggag                                                                                               >  2:2578040/1‑100 (MQ=255)
  aaaaCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGaggagg                                                                                              <  1:656224/100‑1 (MQ=255)
  aaaaCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGaggagg                                                                                              <  2:298809/100‑1 (MQ=255)
   aaaCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAggagga                                                                                             <  2:4438605/100‑1 (MQ=255)
     aCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAggaggaat                                                                                           <  2:2735725/100‑3 (MQ=255)
      ccGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTa                                                                                          <  1:2603511/100‑1 (MQ=255)
       cgcgACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTAc                                                                                         >  2:2339024/1‑100 (MQ=255)
        gcgACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACg                                                                                        >  1:4881481/1‑100 (MQ=255)
        gcgACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACg                                                                                        >  1:1987886/1‑100 (MQ=255)
          gACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGa                                                                                      <  2:3143262/100‑1 (MQ=255)
            cGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGaaa                                                                                    <  2:4120240/100‑1 (MQ=255)
            cGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGaaa                                                                                    >  1:4251589/1‑100 (MQ=255)
              ttGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATc                                                                                  <  1:1685660/100‑1 (MQ=255)
              ttGAAAACCAGAAGCGCCACGCAAGGTAATGTGTTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTAAATCTCGAGGAGGAGTACGGAAATc                                                                                  <  2:3890219/100‑1 (MQ=255)
               tGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCt                                                                                 >  2:2843332/1‑100 (MQ=255)
                gAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTg                                                                                <  2:2390833/100‑1 (MQ=255)
                 aaaaCCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGc                                                                               <  2:1798544/100‑1 (MQ=255)
                  aaaCCAGTAGCGCCACGCAAGGTAGTGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCa                                                                              >  1:241753/1‑100 (MQ=255)
                  aaaCCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCa                                                                              <  1:1344115/100‑1 (MQ=255)
                   aaCCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCaa                                                                             <  1:4833624/100‑1 (MQ=255)
                      cAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGt                                                                          <  1:1063195/100‑1 (MQ=255)
                          aGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTc                                                                      <  1:3086192/100‑1 (MQ=255)
                          aGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTc                                                                      >  1:1577207/1‑100 (MQ=255)
                          aGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTc                                                                      >  1:994009/1‑100 (MQ=255)
                             gcCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTcaga                                                                   >  1:3835745/1‑99 (MQ=255)
                             gcCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTACAACGTTTTcagc                                                                   <  1:1828740/100‑1 (MQ=255)
                                aCGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTcagcagn                                                                <  2:5395112/100‑2 (MQ=255)
                                       gtcaTGTGGTGCCGCAGGTTTCCAATCGGCAGTAGTCGATTTCTGGTGGCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCCGTATTACCt                                                         <  2:1325681/97‑1 (MQ=255)
                                       gTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCt                                                         <  2:584946/100‑1 (MQ=255)
                                        tAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTg                                                        >  2:3663302/1‑100 (MQ=255)
                                          aTGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGc                                                      >  1:3776600/1‑100 (MQ=255)
                                          aTGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGc                                                      <  1:3016829/100‑1 (MQ=255)
                                           tgtgGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCg                                                     >  1:642999/1‑100 (MQ=255)
                                           tgtgGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCg                                                     >  1:929648/1‑100 (MQ=255)
                                             tggtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGaa                                                   >  2:2029888/1‑100 (MQ=255)
                                             tggtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGAACGGACATCTGCGACGTTTTCAGCAGTATTACCTGGCGaa                                                   >  2:5258034/1‑100 (MQ=255)
                                              ggtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAAt                                                  <  1:1521344/100‑1 (MQ=255)
                                               gtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATg                                                 >  1:4010780/1‑100 (MQ=255)
                                                tgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATgc                                                <  1:3418241/100‑1 (MQ=255)
                                                   cGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCa                                             >  2:2431129/1‑100 (MQ=255)
                                                   cGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCa                                             <  1:4507288/100‑1 (MQ=255)
                                                   cGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCa                                             <  1:3579656/100‑1 (MQ=255)
                                                   cGCAGCTTTCCAAGCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTAAGCAGTATTACCTGGCGAATGCGCa                                             >  2:2956286/1‑100 (MQ=255)
                                                    gCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAg                                            <  1:3352917/100‑1 (MQ=255)
                                                     cAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAgg                                           <  1:1497688/100‑1 (MQ=255)
                                                      aGCTATACAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTGTCCGCAGTATTACCTGGCGAATGCGCAGGt                                          >  2:5181608/1‑100 (MQ=255)
                                                       gCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGtt                                         >  1:3072150/1‑100 (MQ=255)
                                                        cTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTc                                        <  1:699529/100‑1 (MQ=255)
                                                         tttCCAATCAGCAGCAGTCGATTTCCGGTGTCAATTTGGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCt                                       >  2:5365577/1‑100 (MQ=255)
                                                          ttCCAATCAGCAGCAGTTGATTTCCGGTGTCAATCTCGAGGAGGAGAACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTg                                      <  2:2133162/100‑1 (MQ=255)
                                                          ttCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTg                                      <  1:1946066/100‑1 (MQ=255)
                                                          ttCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTg                                      >  1:1308284/1‑100 (MQ=255)
                                                           tCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGc                                     <  2:2792929/100‑1 (MQ=255)
                                                           tCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGc                                     <  2:3221381/100‑1 (MQ=255)
                                                           tCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGc                                     <  1:5407208/100‑1 (MQ=255)
                                                           tCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGc                                     <  2:4812713/100‑1 (MQ=255)
                                                            ccAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCa                                    <  2:1290984/100‑1 (MQ=255)
                                                            ccAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCa                                    <  1:213493/100‑1 (MQ=255)
                                                             cAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAg                                   >  1:849233/1‑100 (MQ=255)
                                                             cAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAg                                   <  2:490541/100‑1 (MQ=255)
                                                              aaTCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGa                                  >  1:4816153/1‑100 (MQ=255)
                                                               aTCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGAc                                 >  2:370843/1‑100 (MQ=255)
                                                               aTCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGAc                                 <  1:2036585/100‑1 (MQ=255)
                                                                tCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACg                                <  1:3291278/100‑1 (MQ=255)
                                                                  agcagcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACTGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGc                              <  1:2258810/100‑1 (MQ=255)
                                                                  agcagcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGc                              >  1:1954514/1‑100 (MQ=255)
                                                                  agcagcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGc                              <  1:3759485/100‑1 (MQ=255)
                                                                      gcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAAc                          >  2:1343387/1‑100 (MQ=255)
                                                                      gcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAAc                          >  1:5230422/1‑100 (MQ=255)
                                                                        agTCGATTTCCGGTGTCAATTTCGAGTTGGCGTAAGGAAAAGTGCAACGGTTTCTGCACAATTACCTGACGACTGCGAAAGTTCTCCCGACGTCAAAcac                        >  2:5475315/1‑98 (MQ=255)
                                                                        agTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAAcgc                        >  1:2063076/1‑100 (MQ=255)
                                                                         gTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAAcgcg                       >  2:4559187/1‑100 (MQ=255)
                                                                           cGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAt                     >  2:732364/1‑100 (MQ=255)
                                                                           cGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAt                     <  1:4995974/100‑1 (MQ=255)
                                                                           cGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAt                     <  1:4121802/100‑1 (MQ=255)
                                                                            gATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAtt                    >  1:1069222/1‑100 (MQ=255)
                                                                               ttCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAttttt                 <  1:966057/100‑1 (MQ=255)
                                                                               ttCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAttttt                 <  1:567384/100‑1 (MQ=255)
                                                                               ttCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAttttt                 <  1:95458/100‑1 (MQ=255)
                                                                                tCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAtgtttn                <  2:4660160/100‑2 (MQ=255)
                                                                                tCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTg                <  2:1550421/100‑1 (MQ=255)
                                                                                tCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTg                >  1:4709215/1‑100 (MQ=255)
                                                                                 ccGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGa               <  1:5164856/100‑1 (MQ=255)
                                                                                  cGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGAt              <  1:3038841/100‑1 (MQ=255)
                                                                                  cGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGAt              <  2:5145091/100‑1 (MQ=255)
                                                                                  atctgtCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGAt              <  1:3855193/97‑1 (MQ=255)
                                                                                   ggTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATg             >  1:3801014/1‑100 (MQ=255)
                                                                                   ggTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATg             >  1:2862587/1‑100 (MQ=255)
                                                                                      gtCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGAtagat          >  2:3819893/1‑96 (MQ=255)
                                                                                      gtCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATgcgc          >  2:4130983/1‑100 (MQ=255)
                                                                                       tCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGGAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCt         <  2:120529/100‑1 (MQ=255)
                                                                                        cAACCTCGAGGAGGAGGACGGAAATCTTCAACGTTTTCGGCAGTATTACCTGCCACATGCGCAGGTTCTGCGGACGGCAATCGCGATTTTTGATGCGCTg        >  2:1391957/1‑100 (MQ=255)
                                                                                          aTCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGAt      <  2:2188539/100‑1 (MQ=255)
                                                                                           tctcGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGAtt     <  2:3974307/100‑1 (MQ=255)
                                                                                           tctcGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGAtt     <  2:1785763/100‑1 (MQ=255)
                                                                                           tctcGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGAtt     <  2:2120147/100‑1 (MQ=255)
                                                                                            ctcGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTa    >  1:4788674/1‑100 (MQ=255)
                                                                                              cGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGTTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAAc  >  2:2231486/1‑100 (MQ=255)
                                                                                              cGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAAc  >  1:4927076/1‑100 (MQ=255)
                                                                                              cGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAAc  >  2:2684768/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAAC  >  NC_000913/1139819‑1140012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: