BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,183,048	T→G	L191F (TTA→TTC)	potC ←	spermidine/putrescine ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,183,048	0	T	G	100.0%	667.3 / NA	186	L191F (TTA→TTC)	potC	spermidine/putrescine ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (87/99); total (87/99)

Only 100 of 186 total aligned reads displayed.

CTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCC  >  NC_000913/1182951‑1183144

 tttGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAac                                                                                               <  1:4387311/100‑1 (MQ=255)

 tttGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAac                                                                                               <  2:3518614/100‑1 (MQ=255)

  ttGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAaca                                                                                              >  2:407811/1‑100 (MQ=255)

   tGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAacac                                                                                             >  1:3357762/1‑100 (MQ=255)

   tGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAacac                                                                                             >  2:2730815/1‑100 (MQ=255)

   tGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAacac                                                                                             <  2:4004651/100‑1 (MQ=255)

   tGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAacac                                                                                             <  2:3950506/100‑1 (MQ=255)

     aCCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACAccc                                                                                           <  2:4489969/100‑1 (MQ=255)

     aCCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACAccc                                                                                           <  1:2563417/100‑1 (MQ=255)

      ccATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCa                                                                                          <  2:1856030/100‑1 (MQ=255)

      ccATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCa                                                                                          >  1:225878/1‑100 (MQ=255)

      ccATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCa                                                                                          >  2:2170820/1‑100 (MQ=255)

       cATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAg                                                                                         <  2:2308422/100‑1 (MQ=255)

       cATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAg                                                                                         >  1:563600/1‑100 (MQ=255)

          cGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGccc                                                                                      <  2:2599166/100‑1 (MQ=255)

           gAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCccg                                                                                     >  2:66627/1‑100 (MQ=255)

             aTAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCccgcc                                                                                   <  1:4658712/100‑1 (MQ=255)

             aTAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCccgcc                                                                                   >  1:3196886/1‑100 (MQ=255)

             aTAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCccgcc                                                                                   >  2:4112082/1‑100 (MQ=255)

              tAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCccgccg                                                                                  >  1:4202096/1‑100 (MQ=255)

                aaTTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCgccgcc                                                                                <  1:129694/100‑1 (MQ=255)

                aaTTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACAGCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCgccgcc                                                                                <  2:2659474/100‑1 (MQ=255)

                 aTTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCa                                                                               >  1:5526189/1‑100 (MQ=255)

                  tttttAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCAc                                                                              <  2:3167326/100‑1 (MQ=255)

                       aaCGGCAGAATTTCATCACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTg                                                                         <  1:4578026/100‑1 (MQ=255)

                        aCGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTgg                                                                        <  1:1094984/100‑1 (MQ=255)

                        aCGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTgg                                                                        <  1:2988299/100‑1 (MQ=255)

                        aCGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTgg                                                                        <  2:2129175/100‑1 (MQ=255)

                         cGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGc                                                                       >  1:905055/1‑100 (MQ=255)

                            cAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCAtt                                                                    <  2:3539959/100‑1 (MQ=255)

                             aGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTg                                                                   <  1:4275348/100‑1 (MQ=255)

                              gAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGc                                                                  <  1:1780874/100‑1 (MQ=255)

                              gAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGc                                                                  >  2:2837180/1‑100 (MQ=255)

                               aaTTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGcc                                                                 >  2:4714699/1‑100 (MQ=255)

                                  ttCATAACTTGGTCCGTTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGt                                                              <  1:2732300/100‑1 (MQ=255)

                                    cATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTgg                                                            <  1:5265022/100‑1 (MQ=255)

                                     aTAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGc                                                           <  1:275633/100‑1 (MQ=255)

                                     aTAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGc                                                           <  1:2248363/100‑1 (MQ=255)

                                     aTAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGc                                                           <  1:160386/100‑1 (MQ=255)

                                     aTAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGc                                                           >  1:4601980/1‑100 (MQ=255)

                                     aTAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGc                                                           <  1:3876374/100‑1 (MQ=255)

                                      tAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCa                                                          >  1:1517061/1‑100 (MQ=255)

                                        aCTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGa                                                        >  1:105434/1‑100 (MQ=255)

                                        aCTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGa                                                        >  2:3625041/1‑100 (MQ=255)

                                         cTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGaa                                                       >  1:195677/1‑100 (MQ=255)

                                            ggTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGa                                                    >  1:3661265/1‑100 (MQ=255)

                                            ggTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGa                                                    >  2:2041056/1‑100 (MQ=255)

                                                cGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACACAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGAtttt                                                >  2:281176/1‑100 (MQ=255)

                                                  gTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTc                                              <  1:3844652/100‑1 (MQ=255)

                                                   tGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCg                                             <  2:5218817/100‑1 (MQ=255)

                                                    gACGAACGAAGAAACTACCACATCGTCCATCGTCAGGGTAAATCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGc                                            <  2:5492214/100‑1 (MQ=255)

                                                    gACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGc                                            <  2:3107369/100‑1 (MQ=255)

                                                    gACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGc                                            >  1:1336997/1‑100 (MQ=255)

                                                    gACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGc                                            <  2:643587/100‑1 (MQ=255)

                                                      cgaacgaaGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAg                                          >  1:743475/1‑100 (MQ=255)

                                                        aacgaaGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGaa                                        >  1:315210/1‑100 (MQ=255)

                                                         acgaaGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCATAAt                                       <  2:5044475/100‑1 (MQ=255)

                                                         acgaaGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAAt                                       <  1:2142418/100‑1 (MQ=255)

                                                           gaagaaACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATgg                                     <  2:1717040/100‑1 (MQ=255)

                                                           gaagaaACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATgg                                     >  1:3720623/1‑100 (MQ=255)

                                                           gaagaaACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATgg                                     <  1:2056130/100‑1 (MQ=255)

                                                           gaagaaACTACCACATCGTCCAGCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATgg                                     <  2:3871130/100‑1 (MQ=255)

                                                            aagaaACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGt                                    >  1:4049661/1‑100 (MQ=255)

                                                            aagaaACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGt                                    >  2:3413101/1‑100 (MQ=255)

                                                             agaaACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTa                                   >  2:3287166/1‑100 (MQ=255)

                                                              gaaACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTaa                                  >  2:3732553/1‑100 (MQ=255)

                                                                  cTACCACATCGTCCATAGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGTTGTTTGTTAAATTc                              >  2:5327633/1‑100 (MQ=255)

                                                                     ccACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCt                           <  1:5166155/100‑1 (MQ=255)

                                                                     ccACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCt                           <  1:3481935/100‑1 (MQ=255)

                                                                      cacaTCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTg                          <  1:281009/100‑1 (MQ=255)

                                                                      cacaGCGTCCATCGACAGGGGAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAGTGATTTTTCGCAGAATGGTAAATTCCCTg                          <  2:2559699/100‑1 (MQ=255)

                                                                       acaTCTTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCCGGCATCGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTgg                         >  1:2953550/1‑100 (MQ=255)

                                                                       acaTCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTgg                         <  1:686732/100‑1 (MQ=255)

                                                                       acaTCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTgg                         <  2:4342632/100‑1 (MQ=255)

                                                                        caTCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGc                        <  1:635752/100‑1 (MQ=255)

                                                                           cGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCAcc                     <  1:5453277/100‑1 (MQ=255)

                                                                               cATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCgaga                 <  2:2219399/100‑1 (MQ=255)

                                                                                aTCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGAt                >  1:687552/1‑100 (MQ=255)

                                                                                  cGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCt              <  1:3245828/100‑1 (MQ=255)

                                                                                  cGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCt              >  1:2737621/1‑100 (MQ=255)

                                                                                  cGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCt              >  1:5014619/1‑100 (MQ=255)

                                                                                   gACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCtt             >  1:3385360/1‑100 (MQ=255)

                                                                                    aCAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCttt            >  2:1299085/1‑100 (MQ=255)

                                                                                    aCAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCttt            >  2:595548/1‑100 (MQ=255)

                                                                                       gggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgc         <  1:2646178/100‑1 (MQ=255)

                                                                                       gggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgc         <  2:2648681/100‑1 (MQ=255)

                                                                                       gggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgc         >  1:3052813/1‑100 (MQ=255)

                                                                                       gggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgc         <  2:2991211/100‑1 (MQ=255)

                                                                                       gggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgc         >  2:5177094/1‑100 (MQ=255)

                                                                                        ggTAAAGCTGAACACCCAGCCCGCCGCCCCCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGTATAATGGTAAATTCGCTGGCACCGAGATCTTTcgcc        <  2:955966/100‑1 (MQ=255)

                                                                                        ggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgcc        <  2:4164016/100‑1 (MQ=255)

                                                                                        ggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgcc        >  1:3704609/1‑100 (MQ=255)

                                                                                         gTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgccg       >  2:372284/1‑100 (MQ=255)

                                                                                          tAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgccgc      >  2:2898252/1‑100 (MQ=255)

                                                                                           aaaGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCt     <  2:3716065/100‑1 (MQ=255)

                                                                                            aaGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCtt    >  2:4816620/1‑100 (MQ=255)

                                                                                            aaGCTGAACAACCAGCACGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGTACCGAGATCTTTCGCCGCtt    >  1:4878032/1‑100 (MQ=255)

                                                                                             aGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTc   <  1:3710552/100‑1 (MQ=255)

                                                                                             aGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTc   >  1:4120287/1‑100 (MQ=255)

                                                                                              gCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTcc  >  1:3806451/1‑100 (MQ=255)

CTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCC  >  NC_000913/1182951‑1183144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`