Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,216,638 T→G intergenic (+3/‑110) ariR → / → ymgC RcsB connector protein for regulation of biofilm and acid‑resistance/Blue light, low temperature and stress induced protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,216,6380TG98.7% 835.2 / ‑2.5 237intergenic (+3/‑110)ariR/ymgCRcsB connector protein for regulation of biofilm and acid‑resistance/Blue light, low temperature and stress induced protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (118/114);  total (121/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 237 total aligned reads displayed.

TACACTCCTTGGAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTT  >  NC_000913/1216541‑1216734
                                                                                                 |                                                                                                
   acTCCTTGGAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAat                                                                                             >  2:3428420/1‑100 (MQ=255)
    cTCCTTGGAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAatt                                                                                            >  2:3794084/1‑100 (MQ=255)
    cTCCGTGGAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGATGCGATACACAGCTGATGATATGTAACTACTAatt                                                                                            >  2:4647504/1‑100 (MQ=255)
     tCCTTGGAAACAACAAGTGATATTCTCAAAGATGATGTGATTAGAAAAACAATGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAatta                                                                                           <  2:4859103/100‑1 (MQ=255)
      ccTTGGAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAAGCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAattat                                                                                          <  1:3126155/100‑1 (MQ=255)
       cTTGGAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAattatt                                                                                         >  2:1869553/1‑100 (MQ=255)
        ttGGAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAAttatta                                                                                        <  1:1010834/100‑1 (MQ=255)
        ttGGAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAAttatta                                                                                        <  1:4904194/100‑1 (MQ=255)
          ggAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAg                                                                                      <  2:5094048/100‑1 (MQ=255)
          ggAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAg                                                                                      >  1:4397209/1‑100 (MQ=255)
          ggAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAg                                                                                      <  2:4914762/100‑1 (MQ=255)
          ggAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAg                                                                                      <  1:1476939/100‑1 (MQ=255)
           gAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGt                                                                                     >  1:4103877/1‑100 (MQ=255)
           gAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGt                                                                                     >  2:2562135/1‑100 (MQ=255)
            aaaCAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGta                                                                                    >  1:2913131/1‑100 (MQ=255)
              acaacaAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGtata                                                                                  >  2:786158/1‑100 (MQ=255)
                aacaaGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAg                                                                                >  1:4952432/1‑100 (MQ=255)
                aacaaGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAg                                                                                <  1:3460576/100‑1 (MQ=255)
                    aGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTta                                                                            <  1:244784/100‑1 (MQ=255)
                     gTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTtat                                                                           >  1:3186525/1‑100 (MQ=255)
                      tGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTtata                                                                          <  2:4705639/100‑1 (MQ=255)
                       gATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAg                                                                         <  1:4058265/100‑1 (MQ=255)
                       gATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAg                                                                         <  1:1092291/100‑1 (MQ=255)
                         tatTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGaa                                                                       >  2:3515190/1‑100 (MQ=255)
                         tatTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGaa                                                                       >  2:1090027/1‑100 (MQ=255)
                         tatTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGaa                                                                       >  1:2673126/1‑100 (MQ=255)
                         tatTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATACGTAACTGCTAATTATTAAGTATAAGTTTATAGaa                                                                       >  1:2079865/1‑100 (MQ=255)
                           ttCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGaaan                                                                     <  1:462609/100‑2 (MQ=255)
                           ttCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGaaaa                                                                     >  2:3013620/1‑100 (MQ=255)
                            tctcAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACAGAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAAc                                                                    <  2:2616044/100‑1 (MQ=255)
                            tctcAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAAc                                                                    <  1:4584571/100‑1 (MQ=255)
                            actcAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAAc                                                                    <  2:553872/99‑1 (MQ=255)
                               cAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACtca                                                                 >  2:2183370/1‑100 (MQ=255)
                               cAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACtca                                                                 <  2:1396128/100‑1 (MQ=255)
                                  aGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACtcattc                                                              <  1:2887588/100‑1 (MQ=255)
                                    cTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACtcattcat                                                            >  2:1566346/1‑100 (MQ=255)
                                     tGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATc                                                           <  2:5310095/100‑1 (MQ=255)
                                     tGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATc                                                           <  1:3797503/100‑1 (MQ=255)
                                       aTGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCtt                                                         <  1:455369/100‑1 (MQ=255)
                                         gtgATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTAt                                                       >  2:4375984/1‑100 (MQ=255)
                                         gtgATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACCCATTCATCTTAt                                                       >  1:3508709/1‑100 (MQ=255)
                                          tgATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTGTTAAGTATAAGTTTATAGAAAACTCATTCATCTTAtt                                                      >  1:2309635/1‑100 (MQ=255)
                                          tgATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTAtt                                                      >  2:1500363/1‑100 (MQ=255)
                                          tgATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTAtt                                                      >  2:3818545/1‑100 (MQ=255)
                                           gATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTAttt                                                     >  2:3184089/1‑100 (MQ=255)
                                            aTTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTAtttt                                                    <  2:5302798/100‑1 (MQ=255)
                                              tAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTtg                                                  <  1:5300850/100‑1 (MQ=255)
                                                gAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTtgtc                                                >  2:368336/1‑100 (MQ=255)
                                                gAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTtgtc                                                <  1:1632390/100‑1 (MQ=255)
                                                gAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTAGTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTtgtc                                                <  1:4740431/100‑1 (MQ=255)
                                                 aaaaaCACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTtgtct                                               <  1:2241066/100‑1 (MQ=255)
                                                     acacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCg                                           <  2:734517/100‑1 (MQ=255)
                                                      cacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGc                                          <  2:684613/100‑1 (MQ=255)
                                                      cacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGc                                          >  1:3799419/1‑100 (MQ=255)
                                                        gtGGACATCGTGTCGCGATACACAGCTGATGATATGTAACTGCTACTTATTACGTATAAGTTTATAGAAAACTTATTCATCTTATATTTGTCTGTCGCtt                                        <  2:5136433/99‑1 (MQ=255)
                                                         tGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCttt                                       <  2:1204071/100‑1 (MQ=255)
                                                          ggAAATCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTa                                      >  2:53696/1‑100 (MQ=255)
                                                            aaaTCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGa                                    <  2:1772247/100‑1 (MQ=255)
                                                             aaTCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGAc                                   <  2:3995669/100‑1 (MQ=255)
                                                             aaTCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGAc                                   <  2:1037732/100‑1 (MQ=255)
                                                               tCGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACta                                 >  2:2497687/1‑100 (MQ=255)
                                                                cGTGTTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACtat                                >  2:2120302/1‑100 (MQ=255)
                                                                  tgtTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATac                              >  1:2108218/1‑100 (MQ=255)
                                                                  tgtTGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATac                              <  1:5067764/100‑1 (MQ=255)
                                                                    ttGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATacac                            >  2:2382227/1‑100 (MQ=255)
                                                                     tGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATAcaca                           >  2:548829/1‑100 (MQ=255)
                                                                     tGCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATAcaca                           <  1:274439/100‑1 (MQ=255)
                                                                      gCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAg                          >  2:846188/1‑100 (MQ=255)
                                                                      gCGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAg                          >  2:2702279/1‑100 (MQ=255)
                                                                       cGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTTTCTGTCTCTTTAGACTATACACAGc                         >  2:2965733/1‑100 (MQ=255)
                                                                       cGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGc                         >  1:2396636/1‑100 (MQ=255)
                                                                       cGATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGc                         <  2:3476183/100‑1 (MQ=255)
                                                                        gATACACAGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCa                        >  1:2533504/1‑100 (MQ=255)
                                                                            cacaGCTGATGATATGTAACTGCTAATTATTTAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAAt                    >  2:2666927/1‑100 (MQ=255)
                                                                            cacaGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAAt                    >  2:4521004/1‑100 (MQ=255)
                                                                             acaGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAAtt                   >  1:3620363/1‑100 (MQ=255)
                                                                              caGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAAttt                  <  1:1519539/100‑1 (MQ=255)
                                                                               aGCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAAtttt                 >  2:3422729/1‑100 (MQ=255)
                                                                                gCTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTATACTATACACAGCATAATTTTa                >  2:2049202/1‑100 (MQ=255)
                                                                                 cTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTAt               <  2:2318601/100‑1 (MQ=255)
                                                                                 cTGATGATATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTAt               >  2:516382/1‑100 (MQ=255)
                                                                                  tgatgaTATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTAtt              <  1:3879711/100‑1 (MQ=255)
                                                                                  tgatgaTATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTAtt              <  1:1890494/100‑1 (MQ=255)
                                                                                  tgatgaTATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTAtt              <  1:577274/100‑1 (MQ=255)
                                                                                   gatgatATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTg             <  2:541735/100‑1 (MQ=255)
                                                                                   gatgatATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTg             >  2:2248983/1‑100 (MQ=255)
                                                                                   gatgatATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTg             <  1:4223070/100‑1 (MQ=255)
                                                                                   gatgatATATAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTTTTTTTTTTTTTAGACTATACACAGCATAATTTTAttt             >  2:5279196/1‑99 (MQ=255)
                                                                                      gatATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGt          >  1:2269255/1‑100 (MQ=255)
                                                                                      gatATGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGt          <  2:628606/100‑1 (MQ=255)
                                                                                       atatGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGtt         <  2:2936541/100‑1 (MQ=255)
                                                                                       atatGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTCTATTGGGtt         <  1:5436083/100‑1 (MQ=255)
                                                                                          tGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAat      <  1:1042316/100‑1 (MQ=255)
                                                                                          tGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAat      >  2:4337787/1‑100 (MQ=255)
                                                                                          tGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAat      >  2:1738753/1‑100 (MQ=255)
                                                                                          tGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAat      >  1:3269301/1‑100 (MQ=255)
                                                                                          tGTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTCTCTGTCGCTTTCGACTATACACAGCAGAATTTTATTCGGTTAat      >  1:5110442/1‑100 (MQ=255)
                                                                                           gTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAata     >  1:728507/1‑100 (MQ=255)
                                                                                           gTAACTGCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAata     <  2:905542/100‑1 (MQ=255)
                                                                                              aCTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATAttt  >  2:1331053/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TACACTCCTTGGAAACAACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTT  >  NC_000913/1216541‑1216734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: