Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,290,933 T→G L231R (CTT→CGT)  rssB → PcnB‑degradosome interaction factor; response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,290,9330TG99.1% 762.6 / ‑6.0 214L231R (CTT→CGT) rssBPcnB‑degradosome interaction factor; response regulator
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (118/93);  total (119/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

GCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCT  >  NC_000913/1290836‑1291029
                                                                                                 |                                                                                                
 cATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGc                                                                                               >  1:1171994/1‑100 (MQ=255)
 cATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGc                                                                                               <  1:1878658/100‑1 (MQ=255)
 cATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGc                                                                                               >  1:3757599/1‑100 (MQ=255)
   ttttATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGcac                                                                                             <  2:3691611/100‑1 (MQ=255)
   ttttATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGcac                                                                                             <  1:4782372/100‑1 (MQ=255)
    tttATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGcaca                                                                                            >  1:138439/1‑100 (MQ=255)
     ttATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCacac                                                                                           >  1:497355/1‑100 (MQ=255)
      tATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACAcc                                                                                          <  2:2781819/100‑1 (MQ=255)
      tATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACAcc                                                                                          >  1:642884/1‑100 (MQ=255)
       aTTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCa                                                                                         >  2:3393736/1‑100 (MQ=255)
          gCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCaaaa                                                                                      <  1:2790534/100‑1 (MQ=255)
           ccTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAAt                                                                                     >  2:2687829/1‑100 (MQ=255)
           ccTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAAt                                                                                     <  2:2333541/100‑1 (MQ=255)
           ccTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAAt                                                                                     <  1:3182030/100‑1 (MQ=255)
           ccTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAAt                                                                                     >  2:4424048/1‑100 (MQ=255)
            cTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATc                                                                                    >  1:5486235/1‑100 (MQ=255)
               gATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAAc                                                                                 <  2:2276225/100‑1 (MQ=255)
               gATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATGACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAAc                                                                                 <  1:5164424/100‑1 (MQ=255)
                  gTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGt                                                                              >  1:887192/1‑100 (MQ=255)
                  gTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGt                                                                              <  2:1283953/100‑1 (MQ=255)
                    cACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGGATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTg                                                                            >  1:4986149/1‑100 (MQ=255)
                    cACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTg                                                                            >  1:3266367/1‑100 (MQ=255)
                    cACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTg                                                                            >  2:2055798/1‑100 (MQ=255)
                    cACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTg                                                                            >  1:1093874/1‑100 (MQ=255)
                    cACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTg                                                                            <  1:3427025/100‑1 (MQ=255)
                     aCCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGc                                                                           <  1:4644421/100‑1 (MQ=255)
                      cccGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGcn                                                                          <  1:293927/100‑2 (MQ=255)
                      cccGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGcc                                                                          <  2:90871/100‑1 (MQ=255)
                        cGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCag                                                                        <  1:3280385/100‑1 (MQ=255)
                          aGCTGGACATAATGGCGTACTTGCTGCCTGGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCagag                                                                      <  1:3335144/100‑1 (MQ=255)
                            cTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGtt                                                                    <  1:97344/100‑1 (MQ=255)
                             tGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTg                                                                   >  2:4089384/1‑100 (MQ=255)
                                 cATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGgcg                                                               >  1:2602197/1‑100 (MQ=255)
                                  aTAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGgcgc                                                              >  2:5493564/1‑100 (MQ=255)
                                   naaTGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCCAAATCCACGGTTGCCACAGTTgcgcgcc                                                             >  1:1649972/2‑99 (MQ=255)
                                     aTGGCGTACTTGCTGCGTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGtt                                                           <  1:2374647/100‑1 (MQ=255)
                                     aTGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGtt                                                           >  2:4267296/1‑100 (MQ=255)
                                     aTGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGtt                                                           <  1:2077885/100‑1 (MQ=255)
                                     aTGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGtt                                                           >  1:2752869/1‑100 (MQ=255)
                                       ggCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTAt                                                         <  1:3746210/100‑1 (MQ=255)
                                       ggCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTAt                                                         >  1:1926795/1‑100 (MQ=255)
                                        gCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTAtt                                                        <  1:3864311/100‑1 (MQ=255)
                                        gCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTAtt                                                        >  1:4628709/1‑100 (MQ=255)
                                        gCGTACTTGCTGCCGTGTTATTACGGGCATTGTTTAACGGGTTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTAtt                                                        <  2:2468494/100‑1 (MQ=255)
                                         cGTACTTGCTGCCTTGTTATGACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGGTTGGCGCGTTATTg                                                       >  2:3186403/1‑100 (MQ=255)
                                          gTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGa                                                      <  2:2353229/100‑1 (MQ=255)
                                            aCTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACCGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTACTGAAg                                                    >  2:2132587/1‑100 (MQ=255)
                                            aCTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAg                                                    <  1:3927102/100‑1 (MQ=255)
                                            aCTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAg                                                    <  2:4801593/100‑1 (MQ=255)
                                            aCTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAg                                                    >  2:4365312/1‑100 (MQ=255)
                                             cTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGTGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGc                                                   >  2:818155/1‑100 (MQ=255)
                                             cTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGc                                                   >  2:1081362/1‑100 (MQ=255)
                                              ttGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCa                                                  <  2:4193976/100‑1 (MQ=255)
                                               tgctgcCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAg                                                 <  1:2515954/100‑1 (MQ=255)
                                                gctgcCTTGTTATTACGCGTATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAgg                                                <  2:4916549/100‑1 (MQ=255)
                                                 ctgcCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGt                                               >  2:4625356/1‑100 (MQ=255)
                                                 ctgcCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGt                                               <  2:76135/100‑1 (MQ=255)
                                                 ctgcCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGt                                               <  1:15326/100‑1 (MQ=255)
                                                   nccTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTaa                                             >  1:176069/2‑100 (MQ=255)
                                                    ccTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTaaa                                            >  1:1341253/1‑100 (MQ=255)
                                                          tattaCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCAttt                                      >  2:1478033/1‑100 (MQ=255)
                                                           attaCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTa                                     >  2:2456282/1‑100 (MQ=255)
                                                            ttaCGCGCATTGTTTAACGGATTATTACATGAACAGCGTGCACACCAAAATCAACGGTTACTAAAGTTGGGCGCGTTATTTAAGCAGGTAAACCATTTAc                                    >  2:1673691/1‑100 (MQ=255)
                                                            ttaCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTAc                                    >  2:1672621/1‑100 (MQ=255)
                                                             taCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACt                                   <  1:1699732/100‑1 (MQ=255)
                                                              aCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACtt                                  >  1:3550728/1‑100 (MQ=255)
                                                               cgcgCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTc                                 <  2:172612/100‑1 (MQ=255)
                                                               cgcgCATTGTTTAACGGATAAATACAGGAACAGCGTGCACACGAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTc                                 >  1:4743740/1‑100 (MQ=255)
                                                                gcgcATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCg                                <  1:4365424/100‑1 (MQ=255)
                                                                 ngcATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGt                               >  1:4422399/2‑100 (MQ=255)
                                                                 cgcATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGt                               >  2:4511832/1‑100 (MQ=255)
                                                                  gcATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTc                              <  1:3839801/100‑1 (MQ=255)
                                                                   cATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCa                             <  1:371107/100‑1 (MQ=255)
                                                                     ttGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAgg                           >  2:4895255/1‑100 (MQ=255)
                                                                      tGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGTTTGCCAGAGTTGGGCGCGGTATTGAAGCAGGTAAACCATTTAGTTCGTCAGGc                          <  2:5071576/100‑1 (MQ=255)
                                                                       gTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGcc                         >  1:4158110/1‑100 (MQ=255)
                                                                       gTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGcc                         >  1:285699/1‑100 (MQ=255)
                                                                       gTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGcc                         <  2:537957/100‑1 (MQ=255)
                                                                       gTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGcc                         >  1:666206/1‑100 (MQ=255)
                                                                          tAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAAt                      >  1:4310450/1‑100 (MQ=255)
                                                                           aaCGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATc                     >  1:2920221/1‑100 (MQ=255)
                                                                           aaCGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATc                     >  1:5321419/1‑100 (MQ=255)
                                                                               gATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGcc                 <  1:2333226/100‑1 (MQ=255)
                                                                               gATTATTACAGGAACAGCGGGCACACCAAAATCAACGGGTGCCAGAGTTGCGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCGGGCCAAGCTGcc                 <  2:5283492/100‑1 (MQ=255)
                                                                                attattACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCg                >  2:144336/1‑100 (MQ=255)
                                                                                attattACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGCGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCg                <  2:5412894/100‑1 (MQ=255)
                                                                                 ttattaCAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCgg               >  2:1171797/1‑100 (MQ=255)
                                                                                 ttattaCAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCgg               <  1:3294697/100‑1 (MQ=255)
                                                                                 ttattaCAGGAACAGCGTGCACACCAAAATCAACGGTTGACAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCAAATCTGACgg               <  2:255523/100‑1 (MQ=255)
                                                                                   attaCAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCgggg             >  2:1338115/1‑100 (MQ=255)
                                                                                    ttaCAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGc            >  1:1409020/1‑100 (MQ=255)
                                                                                     taCAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCa           >  2:1785468/1‑100 (MQ=255)
                                                                                      aCAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAg          >  1:2643707/1‑100 (MQ=255)
                                                                                      aCAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAg          >  2:1955015/1‑100 (MQ=255)
                                                                                         ggAACGGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGttt       >  2:4831222/1‑100 (MQ=255)
                                                                                         ggAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGttt       >  1:766192/1‑100 (MQ=255)
                                                                                         ggAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGttt       >  2:3303188/1‑100 (MQ=255)
                                                                                          gAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTc      >  2:2954212/1‑100 (MQ=255)
                                                                                            aCAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCg    >  2:349397/1‑100 (MQ=255)
                                                                                             cAGCGTGCACACCATAATCAACGGTTGCCAGAGTTGGGTGTGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCg  <  2:5030810/100‑2 (MQ=255)
                                                                                                 |                                                                                                
GCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCT  >  NC_000913/1290836‑1291029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: