Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,598,136 A→C intergenic (‑149/+481) yneO ← / ← lsrK pseudogene, AidA homolog/autoinducer‑2 (AI‑2) kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,598,1360AC97.0% 807.6 / 3.3 232intergenic (‑149/+481)yneO/lsrKpseudogene, AidA homolog/autoinducer‑2 (AI‑2) kinase
Reads supporting (aligned to +/- strand):  ref base A (3/4);  new base C (139/86);  total (142/90)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

AATACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTT  >  NC_000913/1598040‑1598232
                                                                                                |                                                                                                
aaTACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGAc                                                                                               <  2:4854947/100‑1 (MQ=255)
 aTACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACa                                                                                              >  1:818738/1‑100 (MQ=255)
  tACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACAt                                                                                             >  1:3893907/1‑100 (MQ=255)
    ccTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTa                                                                                           >  2:3680475/1‑100 (MQ=255)
       gTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTaaaa                                                                                        >  1:1105645/1‑100 (MQ=255)
       gTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTaaaa                                                                                        <  1:1112938/100‑1 (MQ=255)
            gTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGaa                                                                                   >  1:137317/1‑100 (MQ=255)
            gTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGaa                                                                                   >  2:507069/1‑100 (MQ=255)
             tGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGaaa                                                                                  >  1:4601393/1‑100 (MQ=255)
              gCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAAc                                                                                 <  2:1499002/100‑1 (MQ=255)
              gCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAAc                                                                                 >  1:27672/1‑100 (MQ=255)
              gCAACTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAAc                                                                                 <  2:30378/100‑1 (MQ=255)
               cAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACt                                                                                >  2:3855539/1‑100 (MQ=255)
                aaTTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACtt                                                                               >  2:3378325/1‑100 (MQ=255)
                 aTTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACtta                                                                              >  1:429250/1‑100 (MQ=255)
                 aTTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACtta                                                                              >  1:3270137/1‑100 (MQ=255)
                     cATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTaa                                                                          >  1:2034766/1‑100 (MQ=255)
                     cATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTaa                                                                          <  2:3469460/100‑1 (MQ=255)
                      atatCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTaaa                                                                         <  2:1765113/100‑1 (MQ=255)
                       tatCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAAt                                                                        >  2:5151880/1‑100 (MQ=255)
                        atCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAAtt                                                                       >  1:1583734/1‑100 (MQ=255)
                        atCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAAtt                                                                       >  1:4764229/1‑100 (MQ=255)
                         tCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTg                                                                      <  2:4515861/100‑1 (MQ=255)
                         tCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTg                                                                      >  1:1713939/1‑100 (MQ=255)
                         tCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTg                                                                      <  1:4771254/100‑1 (MQ=255)
                         tCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTg                                                                      <  2:3932648/100‑1 (MQ=255)
                         tCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTg                                                                      <  2:2616987/100‑1 (MQ=255)
                         tCACGCGATAGATTCTATTCATATAAACGCTACATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTACATTg                                                                      >  1:4472567/1‑100 (MQ=255)
                           aCGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTc                                                                    >  1:2883586/1‑100 (MQ=255)
                           aCGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTc                                                                    >  2:1168255/1‑100 (MQ=255)
                              cgATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCaga                                                                 <  2:401168/100‑1 (MQ=255)
                              cgATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCaga                                                                 >  1:4671711/1‑100 (MQ=255)
                                  aGATTCTATTCATATAAACGCTCCATATACAAACAATCCAGCCACGAAAGAGATGAGTCGTCCGACCTTAAAAATGAAACTAATTACATTCTCAGCGGTc                                                             >  2:2495221/1‑100 (MQ=255)
                                  aGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTc                                                             <  1:1511203/100‑1 (MQ=255)
                                   gATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCt                                                            >  1:5119329/1‑100 (MQ=255)
                                    aTTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTg                                                           >  1:3014824/1‑100 (MQ=255)
                                     ttCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGt                                                          >  2:3493668/1‑100 (MQ=255)
                                       cTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTAt                                                        >  1:3674673/1‑100 (MQ=255)
                                        tATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTAtt                                                       <  2:2158078/100‑1 (MQ=255)
                                         aTTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTATATTg                                                      >  2:4201044/1‑100 (MQ=255)
                                          ttCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGa                                                     >  1:4072504/1‑100 (MQ=255)
                                          ttCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGa                                                     >  2:3925352/1‑100 (MQ=255)
                                          ttCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGa                                                     >  1:5371809/1‑100 (MQ=255)
                                          ttCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGa                                                     >  2:1832043/1‑100 (MQ=255)
                                            cATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGACACTTATTAAATTGTCAGAGGTCTGTATTGAgt                                                   >  1:3099164/1‑100 (MQ=255)
                                            cATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAgt                                                   >  2:2388872/1‑100 (MQ=255)
                                            cATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAgt                                                   <  1:1010471/100‑1 (MQ=255)
                                             atatAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAgtg                                                  <  2:1377074/100‑1 (MQ=255)
                                             atatAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAgtg                                                  >  2:321227/1‑100 (MQ=255)
                                             atatAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAgtg                                                  >  1:3329632/1‑100 (MQ=255)
                                              tataAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGGgtga                                                 >  2:3545375/1‑99 (MQ=255)
                                              tataAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAgtgt                                                 >  1:912210/1‑100 (MQ=255)
                                                  aaCGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGt                                             >  1:2480874/1‑100 (MQ=255)
                                                      cTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTACTTGGa                                         <  2:4831219/100‑1 (MQ=255)
                                                      cTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGa                                         >  1:1924904/1‑100 (MQ=255)
                                                       nccATATACAAACAATACAGCCACGAAAGAGATGAGTCTTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAg                                        >  1:5422944/2‑100 (MQ=255)
                                                        ccATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAgg                                       >  1:3573145/1‑100 (MQ=255)
                                                        ccATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAgg                                       >  1:3398392/1‑100 (MQ=255)
                                                        ccATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAgg                                       >  1:2180605/1‑100 (MQ=255)
                                                        ccATATACAAACAACACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAgg                                       <  1:2520125/100‑1 (MQ=255)
                                                         cATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGt                                      <  2:5354506/100‑1 (MQ=255)
                                                          atatACAAACAATGCAGCCACGAAAGAGATGCGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTg                                     <  2:4300050/100‑1 (MQ=255)
                                                           tataCAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTgg                                    <  1:910523/100‑1 (MQ=255)
                                                           tataCAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTgg                                    >  1:5434931/1‑100 (MQ=255)
                                                             taCAAACAATACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGa                                  >  2:1352599/1‑100 (MQ=255)
                                                               caaacaaTACAGCCACGAAAGAGCTGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGCGTGTTAGTTGGAGGTGGGAAg                                >  2:1611688/1‑100 (MQ=255)
                                                                aaacaaTACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAgg                               <  1:4115820/100‑1 (MQ=255)
                                                                aaacaaTACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAgg                               >  1:3707274/1‑100 (MQ=255)
                                                                 aacaaTACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGgt                              >  1:697148/1‑100 (MQ=255)
                                                                 aacaaTACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGgt                              <  1:5058191/100‑1 (MQ=255)
                                                                 aacaaTACAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGgt                              <  2:898847/100‑1 (MQ=255)
                                                                  acaaTACAGCCACGACAGAGATGAGTCATCCGACATTAAAACTGAAACATATTAAATCGTCAGAGGTCTGTATTGAGTGTTAGTTGGAAGTGGGAAGgtg                             >  2:2218254/1‑100 (MQ=255)
                                                                  acaaTACAGCCACGAAAGAGATGAGGCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGgtg                             <  1:4124372/100‑1 (MQ=255)
                                                                        cAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCTGAGGACTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAAtt                       >  2:2829201/1‑100 (MQ=255)
                                                                        cAGCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAAtt                       >  2:865820/1‑100 (MQ=255)
                                                                          gCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTg                     <  2:3268421/100‑1 (MQ=255)
                                                                          gCCACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAAATTATTAAATTGTCAGAGGTCTGTATCGAGTGTCAGTTGGAGGTGGGAAGGTGTAAATTTg                     >  2:5255936/1‑100 (MQ=255)
                                                                          gCCACGAAAGAGATGAGTCGCCCGACATTAAAAATGAAACTTATTAAATTGTCAGCGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCACTTTg                     >  2:190984/1‑100 (MQ=255)
                                                                            cACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGc                   >  2:4442363/1‑100 (MQ=255)
                                                                            cACGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGc                   <  1:1852776/100‑1 (MQ=255)
                                                                             aCGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCt                  >  2:4989413/1‑100 (MQ=255)
                                                                              cGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTa                 <  1:1184146/100‑1 (MQ=255)
                                                                              cGAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTa                 <  1:877352/100‑1 (MQ=255)
                                                                               gAAAGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTaa                >  2:3884925/1‑100 (MQ=255)
                                                                                 aaGAGATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAAtt              <  2:53648/100‑1 (MQ=255)
                                                                                  agagATGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAAttt             <  2:4582665/100‑1 (MQ=255)
                                                                                   gagaTGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTg            <  2:3428021/100‑1 (MQ=255)
                                                                                    agaTGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGc           <  2:1117973/100‑1 (MQ=255)
                                                                                     gaTGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCt          <  2:4546279/100‑1 (MQ=255)
                                                                                     gaTGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCt          <  1:3811151/100‑1 (MQ=255)
                                                                                      aTGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTa         <  2:943435/100‑1 (MQ=255)
                                                                                       tGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTaa        >  2:2066543/1‑100 (MQ=255)
                                                                                       tGAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTaa        >  1:3230767/1‑100 (MQ=255)
                                                                                        gAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAAt       >  2:1257635/1‑100 (MQ=255)
                                                                                        gAGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAAt       >  1:1701254/1‑100 (MQ=255)
                                                                                         aGTCGTCCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATg      >  1:4408783/1‑100 (MQ=255)
                                                                                          gtcgtcCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGc     >  2:3001821/1‑100 (MQ=255)
                                                                                           tcgtcCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCt    >  1:990812/1‑100 (MQ=255)
                                                                                             gtcCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCttt  >  2:413608/1‑100 (MQ=255)
                                                                                             gtcCGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCttt  >  1:4202592/1‑100 (MQ=255)
                                                                                                |                                                                                                
AATACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTT  >  NC_000913/1598040‑1598232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: