Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,669,235 A→C Y120D (TAT→GAT)  ynfL ← LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,669,2350AC98.5% 697.8 / ‑2.2 199Y120D (TAT→GAT) ynfLLysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base C (107/89);  total (107/92)
Fisher's exact test for biased strand distribution p-value = 9.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

GTGTTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATAATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGATG  >  NC_000913/1669138‑1669331
                                                                                                 |                                                                                                
gtgtTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGTGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAACGGACATCAGGATCAt                                                                                                >  2:317120/1‑100 (MQ=255)
gtgtTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCAt                                                                                                <  1:2187342/100‑1 (MQ=255)
gtgtTACGCAGCAATCCCATATCCAGCGTTCCTTAAATGAGAGGAGCGATTTGCTCGCGAGGGTTCATTTCGCGGGTTTGTAAATAGACATCAGGATCAt                                                                                                <  2:5297970/100‑1 (MQ=255)
  gtTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGAtcatca                                                                                              >  1:1241596/1‑100 (MQ=255)
  gtTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGAtcatca                                                                                              >  2:3867163/1‑100 (MQ=255)
  gtTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGAtcatca                                                                                              <  1:311812/100‑1 (MQ=255)
   ttACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCAc                                                                                             <  2:276118/100‑1 (MQ=255)
   ttACGCAGCAATCCCATATCCAGCGTTCCTTCAATCAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCAc                                                                                             >  2:5359747/1‑100 (MQ=255)
    tACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACg                                                                                            <  2:2834788/100‑1 (MQ=255)
    tACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACg                                                                                            >  2:4822804/1‑100 (MQ=255)
     aCGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACgg                                                                                           >  1:2019989/1‑100 (MQ=255)
      cGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGc                                                                                          >  1:5039898/1‑100 (MQ=255)
      cGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGc                                                                                          >  2:1535797/1‑100 (MQ=255)
      cGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGc                                                                                          <  1:4244156/100‑1 (MQ=255)
      cGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGc                                                                                          >  1:3671823/1‑100 (MQ=255)
       gcagcaATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCg                                                                                         <  1:3993176/100‑1 (MQ=255)
        cagcaATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGa                                                                                        >  2:1871025/1‑100 (MQ=255)
        cagcaATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGa                                                                                        >  2:3115753/1‑100 (MQ=255)
           caATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAAc                                                                                     <  2:1808177/100‑1 (MQ=255)
           caATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAAc                                                                                     <  2:2695090/100‑1 (MQ=255)
             aTCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAg                                                                                   <  1:3179207/100‑1 (MQ=255)
              tCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGc                                                                                  <  2:1589190/100‑1 (MQ=255)
               tccATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCg                                                                                 >  1:1148373/2‑100 (MQ=255)
               gccATATCCCGCGTTCCTTCAATGAGCGGAGAGATTTGCTCGCTATTCTCCATTTCGCGGGTTTGTAAGTGGACATCAGGATCATCACGGGGCAACGGCg                                                                                 <  2:4530940/99‑1 (MQ=255)
               cccATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCg                                                                                 <  1:5418424/100‑1 (MQ=255)
               cccATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCg                                                                                 >  2:4383705/1‑100 (MQ=255)
                ccATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGa                                                                                >  2:3084453/1‑100 (MQ=255)
                  atatCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATa                                                                              >  1:2767246/1‑100 (MQ=255)
                     tCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACg                                                                           >  2:1686029/1‑100 (MQ=255)
                     cacAGCGTTACTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACg                                                                           <  1:2933057/98‑1 (MQ=255)
                      ccAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGt                                                                          <  2:829653/100‑1 (MQ=255)
                       cAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTa                                                                         >  1:2426585/1‑100 (MQ=255)
                       cAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTa                                                                         <  2:2455244/100‑1 (MQ=255)
                         gCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATc                                                                       <  1:4069707/100‑1 (MQ=255)
                         gCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATc                                                                       <  2:1302042/100‑1 (MQ=255)
                           gTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCgg                                                                     <  2:3832413/100‑1 (MQ=255)
                           gTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCgg                                                                     <  2:1777372/100‑1 (MQ=255)
                             tCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGac                                                                   <  2:1191618/100‑1 (MQ=255)
                               cTTCAATTAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAGCAGCGATAACGTATCGGacac                                                                 >  2:3038673/1‑100 (MQ=255)
                               cTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGacac                                                                 <  2:1476313/100‑1 (MQ=255)
                                       agcggagcgATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGaa                                                         >  1:5551378/1‑100 (MQ=255)
                                        gcggagcgATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAAt                                                        <  1:189958/100‑1 (MQ=255)
                                         cggagcgATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATa                                                       <  2:143252/100‑1 (MQ=255)
                                             gcgATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAg                                                   <  1:990243/100‑1 (MQ=255)
                                              cgATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAgg                                                  <  2:2225013/100‑1 (MQ=255)
                                              cgATGTGCTCGCGAGTGTTCAGTTCGCGAGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAgg                                                  <  2:4205576/100‑1 (MQ=255)
                                                aTTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAg                                                >  1:242219/1‑100 (MQ=255)
                                                aTTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAg                                                <  2:1215128/100‑1 (MQ=255)
                                                aTTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAg                                                <  1:3902839/100‑1 (MQ=255)
                                                   tGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCcg                                             <  2:546595/100‑1 (MQ=255)
                                                     cTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCcgac                                           >  1:3244883/1‑100 (MQ=255)
                                                     cTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCcgac                                           >  2:2631949/1‑100 (MQ=255)
                                                      tCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGACCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCcgacg                                          <  1:1055557/100‑1 (MQ=255)
                                                       cgcgAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCcgacga                                         >  1:3507478/1‑100 (MQ=255)
                                                         cgAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAg                                       <  2:4728093/100‑1 (MQ=255)
                                                         cgAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAg                                       >  1:4538042/1‑100 (MQ=255)
                                                             tgtTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTaaa                                   <  1:516363/100‑1 (MQ=255)
                                                              nttCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTaaaa                                  >  1:1576088/2‑100 (MQ=255)
                                                              gtTCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGCaaac                                  <  2:2144659/100‑2 (MQ=255)
                                                               ttCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAAc                                 <  2:1034694/100‑1 (MQ=255)
                                                               ttCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAAc                                 >  1:1988735/1‑100 (MQ=255)
                                                               ttCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAAc                                 >  2:3542743/1‑100 (MQ=255)
                                                                tCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAAcc                                <  2:1967156/100‑1 (MQ=255)
                                                                tCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAAcc                                <  2:239839/100‑1 (MQ=255)
                                                                tCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAAcc                                >  1:5503847/1‑100 (MQ=255)
                                                                tCATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAAcc                                >  2:49661/1‑100 (MQ=255)
                                                                 cATTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCa                               >  1:4603299/1‑100 (MQ=255)
                                                                  aTTTCGCGGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCaa                              <  1:2352994/100‑1 (MQ=255)
                                                                   tttCGCGGGTTTGTAAATGGACATAAGGCTCATGACGGCGTAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAAt                             >  2:1971082/1‑100 (MQ=255)
                                                                      cgcgGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATgcg                          >  1:742978/1‑100 (MQ=255)
                                                                      cgcgGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATgcg                          >  1:754392/1‑100 (MQ=255)
                                                                      cgcgGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATgcg                          >  1:1278433/1‑100 (MQ=255)
                                                                      cgcgGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGAAAAACCAATgcg                          >  1:4488160/1‑100 (MQ=255)
                                                                       gcgGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATgcgc                         >  1:2328082/1‑100 (MQ=255)
                                                                       gcgGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATgcgc                         <  2:5193479/100‑1 (MQ=255)
                                                                        cgGGTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCa                        <  1:1477528/100‑1 (MQ=255)
                                                                          ggTTTGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAAc                      <  2:1056990/100‑1 (MQ=255)
                                                                           gTTTGTAAATGGACATCAGGTTCATAACAGCGAAACAGCGATAACGTATCGGACACCGCCCAAAAACAATGAGCCGACGACGTACAACCACGCCGCAAcc                     >  2:4426653/1‑99 (MQ=255)
                                                                            tttGTAAATGGACATCA‑GATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTcc                   >  2:1376694/1‑100 (MQ=255)
                                                                             ttGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTcc                   >  1:1755371/1‑100 (MQ=255)
                                                                              tGTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTccc                  >  2:396463/1‑100 (MQ=255)
                                                                               gTAAATGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTcccc                 <  1:5460981/100‑1 (MQ=255)
                                                                                  aaTGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGc              >  1:1391047/1‑100 (MQ=255)
                                                                                    tGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCtt            <  2:3787433/100‑1 (MQ=255)
                                                                                    tGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCtt            <  1:940534/100‑1 (MQ=255)
                                                                                    tGGACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCtt            <  1:3195599/100‑1 (MQ=255)
                                                                                     ggACATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTc           <  1:3734458/100‑1 (MQ=255)
                                                                                      atcttCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCa          <  1:3301379/96‑1 (MQ=255)
                                                                                       aCATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCAc         >  2:3457825/1‑100 (MQ=255)
                                                                                        cATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGTCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCAcc        >  1:4884937/1‑100 (MQ=255)
                                                                                        cATCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCAcc        >  1:3136174/1‑100 (MQ=255)
                                                                                         aTCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCAccc       >  1:1498317/1‑100 (MQ=255)
                                                                                         aTCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCAccc       >  2:4791966/1‑100 (MQ=255)
                                                                                          tCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCaagat      >  2:3270727/1‑96 (MQ=255)
                                                                                          tCAGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCt      >  1:3061251/1‑100 (MQ=255)
                                                                                            aGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGa    >  1:894823/1‑100 (MQ=255)
                                                                                            aGGATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGa    >  1:1847595/1‑100 (MQ=255)
                                                                                             ggATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGAt   >  2:842449/1‑100 (MQ=255)
                                                                                             ggATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGAt   >  2:5147389/1‑100 (MQ=255)
                                                                                              gATCATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGATg  >  2:3361740/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GTGTTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCGAGTGTTCATTTCGCGGGTTTGTAAATGGACATCAGGATAATCACGGCGAAACAGCGATAACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGATG  >  NC_000913/1669138‑1669331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑