BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,929	T→G	L411R (CTG→CGG)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,929	0	T	G	99.4%	653.3 / ‑4.2	180	L411R (CTG→CGG)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (86/93); total (87/93)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 180 total aligned reads displayed.

CAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGC  >  NC_000913/1756832‑1757025

cAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCATTGAAAAAAAAAAAAAAAACGGCTCATCAGTTGGTACGGa                                                                                                <  2:1672660/100‑1 (MQ=255)

 aGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAACCGGCTCATCAGTTGGTACGGAg                                                                                               >  2:4954205/1‑100 (MQ=255)

 aGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAg                                                                                               <  2:4996434/100‑1 (MQ=255)

  gggCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGc                                                                                              >  1:5306553/1‑100 (MQ=255)

   ggCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCa                                                                                             >  1:231613/1‑100 (MQ=255)

     cGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGAACACAAAAGAAAAAACGGCTCATCAGTTGGTACGGAGCaaa                                                                                           <  2:198782/100‑1 (MQ=255)

      ggTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAg                                                                                          <  1:4722853/100‑1 (MQ=255)

         aaaTCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCg                                                                                       >  2:671528/1‑100 (MQ=255)

            tCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTtg                                                                                    >  2:2036399/1‑100 (MQ=255)

             cTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTtgt                                                                                   >  2:5451107/1‑100 (MQ=255)

             cTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTtgt                                                                                   >  1:1745826/1‑100 (MQ=255)

             cTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTtgt                                                                                   <  1:2818450/100‑1 (MQ=255)

             cTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTtgt                                                                                   >  1:2615930/1‑100 (MQ=255)

             cTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTtgt                                                                                   >  2:4511424/1‑100 (MQ=255)

               gCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGc                                                                                 <  2:42841/100‑1 (MQ=255)

               gCTCGCACAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGc                                                                                 >  1:1100440/1‑100 (MQ=255)

                cTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAAGGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTtgtgac                                                                                <  2:762474/100‑3 (MQ=255)

                cTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGcc                                                                                >  2:4631454/1‑100 (MQ=255)

                  cgcgCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGc                                                                              >  2:2149464/1‑100 (MQ=255)

                   gcgcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCa                                                                             <  1:5039979/100‑1 (MQ=255)

                    cgcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAg                                                                            <  1:1570064/100‑1 (MQ=255)

                    cgcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAg                                                                            >  2:1012656/1‑100 (MQ=255)

                    cgcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAg                                                                            >  1:830339/1‑100 (MQ=255)

                    cgcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAg                                                                            >  1:1519353/1‑100 (MQ=255)

                     gcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGc                                                                           >  1:4115804/1‑100 (MQ=255)

                       aGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGCTGTGCCGAAGCta                                                                         >  2:5123502/1‑99 (MQ=255)

                        gTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTg                                                                        <  2:3762736/100‑1 (MQ=255)

                        gTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTg                                                                        <  1:4327964/100‑1 (MQ=255)

                        gTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTg                                                                        <  1:4119629/100‑1 (MQ=255)

                          aCGTAAATCCTTCCCGGATGCCACCCGCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGtt                                                                      <  1:5221698/100‑1 (MQ=255)

                           cGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGttn                                                                     <  1:4411918/100‑2 (MQ=255)

                           cGTAAATACTTCCCGGATGCCACCATCCTGGCACTGAAAAAAAAAAAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTa                                                                     <  2:3855782/100‑1 (MQ=255)

                            gTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTaa                                                                    >  1:460857/1‑100 (MQ=255)

                             tAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTaaa                                                                   >  2:3432190/1‑100 (MQ=255)

                              aaaTACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAag                                                                  <  1:3196495/100‑1 (MQ=255)

                              aaaGACTTCCCGTATGCCACCATCCGGGCACTGACCACCAACGAAAAATCGGCTCGTCAGTTTTTACGGAGCAAAGGCGTTGTGCAGGAGCTGGTTTAag                                                                  >  2:252512/1‑100 (MQ=255)

                               aaTACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAaga                                                                 <  1:405390/100‑1 (MQ=255)

                               aaTACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAaga                                                                 <  2:3277202/100‑1 (MQ=255)

                                aTACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAagag                                                                >  1:2199251/1‑100 (MQ=255)

                                   cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATc                                                             <  2:5234549/100‑1 (MQ=255)

                                   cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATc                                                             <  2:3569664/100‑1 (MQ=255)

                                   cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATc                                                             <  2:4058863/100‑1 (MQ=255)

                                   cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATc                                                             >  2:2178579/1‑100 (MQ=255)

                                    ttCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCa                                                            >  1:3097949/1‑100 (MQ=255)

                                      cccGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACt                                                          >  1:1065877/1‑100 (MQ=255)

                                        cGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTc                                                        <  1:460241/100‑1 (MQ=255)

                                        cGGATGCCACCATCCTGGCACAGAAAAAAAAAGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTc                                                        <  2:4781793/100‑1 (MQ=255)

                                          gATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTa                                                      <  1:268215/100‑1 (MQ=255)

                                           aTGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTAc                                                     <  2:3419443/100‑1 (MQ=255)

                                           aTGCCACCATCCTGGCACTGACCACAAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTAc                                                     <  2:4015/100‑1 (MQ=255)

                                              ccaccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtga                                                  <  1:3005161/100‑1 (MQ=255)

                                              ccaccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtga                                                  >  2:4323706/1‑100 (MQ=255)

                                               caccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtgat                                                 >  1:975219/1‑100 (MQ=255)

                                               caccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtgat                                                 >  1:706634/1‑100 (MQ=255)

                                                 ccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtgatga                                               >  1:3749848/1‑100 (MQ=255)

                                                  caTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTgatgat                                              <  1:3813247/100‑1 (MQ=255)

                                                   aTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGAtt                                             <  2:412393/100‑1 (MQ=255)

                                                    tCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGAttt                                            <  2:2590385/100‑1 (MQ=255)

                                                    tCCTGGCAATGAGCACAAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTGCTACTGATGAttt                                            <  2:3660829/100‑1 (MQ=255)

                                                     ccTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGAtttn                                           <  1:508801/100‑2 (MQ=255)

                                                     ccTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTc                                           <  1:2373975/100‑1 (MQ=255)

                                                     ccTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACCTCTACTGATGATTTc                                           <  2:4182275/100‑1 (MQ=255)

                                                      cTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCt                                          <  2:4570510/100‑1 (MQ=255)

                                                        ggCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTAc                                        >  1:1551814/1‑100 (MQ=255)

                                                         gCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTAcc                                       >  1:2453976/1‑100 (MQ=255)

                                                          cACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCg                                      <  2:2279341/100‑1 (MQ=255)

                                                          cACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCg                                      >  2:1155999/1‑100 (MQ=255)

                                                           aCTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGt                                     >  1:5553321/1‑100 (MQ=255)

                                                            cTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTc                                    >  2:3957968/1‑100 (MQ=255)

                                                             tGACCACCAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAACTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCt                                   <  1:1613347/100‑1 (MQ=255)

                                                             tGACCAACAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCt                                   <  2:1465014/100‑1 (MQ=255)

                                                               accaccAACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTgg                                 >  2:274668/1‑100 (MQ=255)

                                                                ccaccaACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTggg                                >  2:4177201/1‑100 (MQ=255)

                                                                  accaACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTa                              >  2:909857/1‑100 (MQ=255)

                                                                   ccaACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTaa                             <  1:1145666/100‑1 (MQ=255)

                                                                   ccaACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTaa                             <  1:921985/100‑1 (MQ=255)

                                                                    caACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTaaa                            <  1:3663098/100‑1 (MQ=255)

                                                                    caACGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTaaa                            >  1:2970204/1‑100 (MQ=255)

                                                                     aaCGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAg                           <  2:1650943/100‑1 (MQ=255)

                                                                     aaCGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGCTGTGCCGCACCTTGTTTACGAGATCACTTACACTGATGATTTCTACCGTCTGGGCaatg                           >  2:4741929/1‑98 (MQ=255)

                                                                      ccGAAAAAACGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGa                          <  2:2940658/99‑1 (MQ=255)

                                                                              aCGGCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGctc                  <  1:1891343/100‑1 (MQ=255)

                                                                                ggCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTg                <  2:1085769/100‑1 (MQ=255)

                                                                                 gCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGc               >  1:4684647/1‑100 (MQ=255)

                                                                                 gCTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGc               <  2:4651144/100‑1 (MQ=255)

                                                                                 gCTCATCAGTTGGTACGGACCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGTTTTCTACCGTCTGGGTAAAGAACTGGCTCTGc               <  1:4953678/100‑1 (MQ=255)

                                                                                  cTCATCAGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCa              >  1:2160188/1‑100 (MQ=255)

                                                                                    catcaGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCaga            >  1:129248/1‑100 (MQ=255)

                                                                                      tcaGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGc          >  1:5552185/1‑100 (MQ=255)

                                                                                      tcaGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGc          <  1:2146113/100‑1 (MQ=255)

                                                                                       caGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGCACTGGCTCTGCAGAGCg         >  2:1612806/1‑100 (MQ=255)

                                                                                        aGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCgg        >  1:3593024/1‑100 (MQ=255)

                                                                                        aGTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCgg        <  2:3727420/100‑1 (MQ=255)

                                                                                         gTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGt       >  1:219191/1‑100 (MQ=255)

                                                                                         gTTGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGt       >  2:5458767/1‑100 (MQ=255)

                                                                                          ttGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTc      <  2:320823/100‑1 (MQ=255)

                                                                                           tGGTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTGCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCt     >  2:2748259/1‑100 (MQ=255)

                                                                                            ggTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTg    <  1:5256951/100‑1 (MQ=255)

                                                                                             gTACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTgg   <  1:4873863/100‑1 (MQ=255)

                                                                                              tACGGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGc  <  1:3657568/100‑1 (MQ=255)

CAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGC  >  NC_000913/1756832‑1757025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`