Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 246,246 A→C I86S (ATT→AGT)  dinJ ← antitoxin of YafQ‑DinJ toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913246,2460AC98.8% 856.1 / ‑2.3 240I86S (ATT→AGT) dinJantitoxin of YafQ‑DinJ toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base C (121/116);  total (121/119)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

ATTTCAATTTATTCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAAATTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTAATTGATTA  >  NC_000913/246149‑246341
                                                                                                 |                                                                                               
atTTCAATTTATTCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAAAtt                                                                                               <  1:4920836/100‑1 (MQ=255)
  ttCAATTTATTCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTcc                                                                                             >  2:5085579/1‑100 (MQ=255)
  gtggaTTTATTCATATCCTTATGACGCTTTTGTGGAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTcc                                                                                             <  2:4441442/96‑1 (MQ=255)
    cAATTTATTCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTa                                                                                           >  1:3292420/1‑100 (MQ=255)
      aTTTATTCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAAt                                                                                         >  1:333941/1‑100 (MQ=255)
      aTTTATTCATATCATTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAAt                                                                                         <  2:1070534/100‑1 (MQ=255)
       tttATTCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAAtt                                                                                        <  1:2943337/100‑1 (MQ=255)
       tttATTCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAAtt                                                                                        <  2:884594/100‑1 (MQ=255)
        ttATTCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAAttt                                                                                       <  2:828142/100‑1 (MQ=255)
         tATTCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTa                                                                                      >  1:1359333/1‑100 (MQ=255)
         tATTAAAATCCTTAAGATGCTTTAGTGCAAGTTTTACATCCTTGAAATAGTGTCACGATGATTCAATATCCGTTTAAGTCATATTTAAAATCCTAATTTa                                                                                      <  2:3498322/100‑1 (MQ=255)
          aTTCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTAt                                                                                     <  1:1538253/100‑1 (MQ=255)
           ttCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATc                                                                                    >  2:411506/1‑100 (MQ=255)
            tCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCa                                                                                   <  1:1950763/100‑1 (MQ=255)
            tCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCa                                                                                   <  2:607592/100‑1 (MQ=255)
            tCAGATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCa                                                                                   <  2:3589667/100‑1 (MQ=255)
             cATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaa                                                                                  >  1:5005109/1‑100 (MQ=255)
              atatCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaaa                                                                                 >  1:5036601/1‑100 (MQ=255)
              atatCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaaa                                                                                 >  1:654117/1‑100 (MQ=255)
               tatCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaaat                                                                                >  1:442078/1‑100 (MQ=255)
               catCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGGCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaaat                                                                                <  2:5073791/99‑1 (MQ=255)
                atCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaaata                                                                               >  2:227679/1‑100 (MQ=255)
                atCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaaata                                                                               <  2:79328/100‑1 (MQ=255)
                atCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaaata                                                                               <  2:2152523/100‑1 (MQ=255)
                 tCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaaataa                                                                              <  1:2750838/100‑1 (MQ=255)
                 tCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaaataa                                                                              >  2:78383/1‑100 (MQ=255)
                  ccTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaaataaa                                                                             <  1:4692609/100‑1 (MQ=255)
                   cTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCaaataaat                                                                            <  1:2171043/100‑1 (MQ=255)
                      aTGACGCTTTTGTGCAAGTTTTACATCCTTAGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAatcat                                                                         <  1:1647741/100‑1 (MQ=255)
                            cTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCGATATCCCTTTTAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGt                                                                   >  1:3895919/1‑100 (MQ=255)
                            cTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGt                                                                   >  2:3708757/1‑100 (MQ=255)
                             ttttGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTc                                                                  <  2:1486342/100‑1 (MQ=255)
                              tttGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCt                                                                 <  2:3272698/100‑1 (MQ=255)
                              tttGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCt                                                                 <  1:3470506/100‑1 (MQ=255)
                                 gtgCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTg                                                              >  1:4825313/1‑100 (MQ=255)
                                  tgCAAGTTGTACATCCTTTGAATATTGTCCCGAGTATTCAACATCCCTTTGAATCATATTTAACTTCCTATTTAACCAAATAAATCATCGGCATCTTTgg                                                             >  2:5227296/1‑100 (MQ=255)
                                   gCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGc                                                            <  1:3384258/100‑1 (MQ=255)
                                   gCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGc                                                            >  2:3068244/1‑100 (MQ=255)
                                    cAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGcc                                                           >  2:1335729/1‑100 (MQ=255)
                                      aGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCtt                                                         >  1:5374200/1‑100 (MQ=255)
                                       gTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTa                                                        >  2:3161180/1‑100 (MQ=255)
                                       gTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTa                                                        <  1:1435120/100‑1 (MQ=255)
                                       gTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTa                                                        >  1:3867564/1‑100 (MQ=255)
                                        ttttACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTAt                                                       <  2:2107742/100‑1 (MQ=255)
                                         tttACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATg                                                      <  1:5506283/100‑1 (MQ=255)
                                         tttACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATg                                                      <  1:3530155/100‑1 (MQ=255)
                                           tACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGaa                                                    >  1:2279213/1‑100 (MQ=255)
                                             cATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACa                                                  <  2:2289390/100‑1 (MQ=255)
                                              aTCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACAt                                                 >  1:4152781/1‑100 (MQ=255)
                                               tCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATc                                                >  2:1666018/1‑100 (MQ=255)
                                               tCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATc                                                <  2:5312868/100‑1 (MQ=255)
                                                ccTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCa                                               >  2:3227038/1‑100 (MQ=255)
                                                ccTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCa                                               >  1:810890/1‑100 (MQ=255)
                                                ccTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCa                                               <  1:2077299/100‑1 (MQ=255)
                                                 cTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCaa                                              >  2:3153451/1‑100 (MQ=255)
                                                 cTTTGAATATAGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCaa                                              >  2:1810896/1‑100 (MQ=255)
                                                  tttGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCATTGGCCTTATGAACATCAAt                                             >  2:1997395/1‑100 (MQ=255)
                                                  tttGAATATTGTCCCGAGTATTCAAGATCCCTTTGAATCATATTTACCTTCCTAATTTATCAAATTAATCATCGGCGTCTTTGGCCTTATGAACATCAAt                                             <  1:5169754/100‑1 (MQ=255)
                                                    tGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGc                                           >  2:5395/1‑100 (MQ=255)
                                                     gAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGcc                                          >  1:236469/1‑100 (MQ=255)
                                                      aaTATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCa                                         >  2:3311029/1‑100 (MQ=255)
                                                            gTCCCGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCg                                   >  1:1275301/1‑100 (MQ=255)
                                                              cccGAGTATTCAATATCCCTTTGATTCATATTTAACTTCCTAATTTATCAAATAAATCATCGCCGTCTTTGTCCTTATGAACATCAATGCCCGCTTCGCt                                 >  2:2321226/1‑100 (MQ=255)
                                                              cccGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCt                                 >  2:239728/1‑100 (MQ=255)
                                                              cccGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCt                                 >  2:1221580/1‑100 (MQ=255)
                                                               ccGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTg                                >  2:3531015/1‑100 (MQ=255)
                                                               ccGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTg                                >  1:114757/1‑100 (MQ=255)
                                                                cGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGt                               >  2:516194/1‑100 (MQ=255)
                                                                cGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGt                               >  1:976520/1‑100 (MQ=255)
                                                                cGAGTATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGt                               >  1:3725789/1‑100 (MQ=255)
                                                                    tATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGttttt                           <  1:105196/100‑1 (MQ=255)
                                                                    tATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGttttt                           >  2:1861412/1‑100 (MQ=255)
                                                                    tATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGttttt                           <  1:4495452/100‑1 (MQ=255)
                                                                    tATTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGtttta                           >  2:4570271/1‑99 (MQ=255)
                                                                     aTTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTttg                          >  1:5255632/1‑100 (MQ=255)
                                                                     aTTCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTttg                          >  1:1245362/1‑100 (MQ=255)
                                                                      ttCAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTttga                         <  2:4633879/100‑1 (MQ=255)
                                                                       tCAATATCCCTTTGAATCATATTTAAATTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTttgat                        <  1:4193548/100‑1 (MQ=255)
                                                                        cAATATCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTttgatt                       >  2:667770/1‑100 (MQ=255)
                                                                            atCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTttgattgatt                   <  1:675968/100‑1 (MQ=255)
                                                                            atCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTttgattgatt                   >  1:22138/1‑100 (MQ=255)
                                                                             tCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTttgattgattg                  <  2:2804229/100‑1 (MQ=255)
                                                                             tCCCTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTttgattgattg                  >  1:2704405/1‑100 (MQ=255)
                                                                               ccTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGaa                <  2:1657453/100‑1 (MQ=255)
                                                                               ccTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGaa                >  1:4514986/1‑100 (MQ=255)
                                                                                cTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAAt               >  1:3092581/1‑100 (MQ=255)
                                                                                cTTTGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAAt               <  1:1672664/100‑1 (MQ=255)
                                                                                 tttGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATg              <  1:1714877/100‑1 (MQ=255)
                                                                                 tttGAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATg              >  1:2573949/1‑100 (MQ=255)
                                                                                    gAATCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGtt           >  2:1773977/1‑100 (MQ=255)
                                                                                     aaTCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTa          <  1:1888607/100‑1 (MQ=255)
                                                                                       tCATATTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTAAt        <  1:229909/100‑1 (MQ=255)
                                                                                         atatTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTAATTg      <  2:3860301/100‑1 (MQ=255)
                                                                                         atatTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTAATTg      <  2:4966707/100‑1 (MQ=255)
                                                                                         atatTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTAATTg      <  1:2821636/100‑1 (MQ=255)
                                                                                          tatTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTAATTGa     >  2:3897352/1‑100 (MQ=255)
                                                                                          tatTTAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTAATTGa     >  2:1881932/1‑100 (MQ=255)
                                                                                            tttAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTAATTGAtt   >  1:5279905/1‑100 (MQ=255)
                                                                                            tttAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTAATTGAtt   <  2:4348321/100‑1 (MQ=255)
                                                                                             ttAACTTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTAATTGATTa  <  1:2514209/100‑1 (MQ=255)
                                                                                                 |                                                                                               
ATTTCAATTTATTCATATCCTTATGACGCTTTTGTGCAAGTTTTACATCCTTTGAATATTGTCCCGAGTATTCAATATCCCTTTGAATCATATTTAAATTCCTAATTTATCAAATAAATCATCGGCGTCTTTGGCCTTATGAACATCAATGCCAGCTTCGCTGTTTTTGATTGATTGAATGGTTAATTGATTA  >  NC_000913/246149‑246341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: