Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,815,451 T→G F529V (TTT→GTT)  katE → catalase HPII, heme d‑containing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,815,4510TG99.3% 475.9 / ‑4.6 135F529V (TTT→GTT) katEcatalase HPII, heme d‑containing
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (56/78);  total (57/78)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 137 total aligned reads displayed.

GCGCAGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCGA  >  NC_000913/1815354‑1815544
                                                                                                 |                                                                                             
gcgcAGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGtgtt                                                                                             <  2:942954/100‑1 (MQ=255)
  gaaGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCATACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGa                                                                                           <  1:2330955/98‑1 (MQ=255)
   cAGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAg                                                                                          <  2:4784001/100‑1 (MQ=255)
     gCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGtt                                                                                        <  1:1299888/100‑1 (MQ=255)
     gCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAACAGCGCCATATTGTCGATGGTTTCAGTGTTGAGtt                                                                                        >  2:5426033/1‑100 (MQ=255)
      cccATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTa                                                                                       <  2:324029/100‑1 (MQ=255)
      cccATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTa                                                                                       <  2:437949/100‑1 (MQ=255)
       ccATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTaa                                                                                      >  1:3431317/1‑100 (MQ=255)
       ccATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTaa                                                                                      <  1:4196883/100‑1 (MQ=255)
       ccATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTaa                                                                                      >  2:4581517/1‑100 (MQ=255)
        cATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAg                                                                                     <  2:3779592/100‑1 (MQ=255)
        cATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAg                                                                                     <  1:4180343/100‑1 (MQ=255)
        cATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAg                                                                                     >  2:497910/1‑100 (MQ=255)
        cATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAg                                                                                     <  2:2777633/100‑1 (MQ=255)
        cATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAg                                                                                     >  1:3814577/1‑100 (MQ=255)
         aTCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGc                                                                                    <  1:4039079/100‑1 (MQ=255)
          tCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCa                                                                                   <  2:2348973/100‑1 (MQ=255)
              ttGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGt                                                                               <  2:1932551/100‑1 (MQ=255)
              ttGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGt                                                                               >  2:3361323/1‑100 (MQ=255)
                ggCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcg                                                                             >  2:172525/1‑100 (MQ=255)
                ggCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcg                                                                             >  1:322895/1‑100 (MQ=255)
                 gCGAATATTATTCCCATCCGCGCCTGTTCTGGCTAAGTCAGACGCCATTTGAACAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgt                                                                            >  1:4658391/1‑100 (MQ=255)
                  cGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgtt                                                                           <  2:1562133/100‑1 (MQ=255)
                  cGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgtt                                                                           <  2:1373783/100‑1 (MQ=255)
                   gAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgttc                                                                          <  1:4050179/100‑1 (MQ=255)
                    aaTATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgttcg                                                                         <  2:1281589/100‑1 (MQ=255)
                    aaTATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgttcg                                                                         <  1:44670/100‑1 (MQ=255)
                    aaTATTATCCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgttcg                                                                         <  1:5532128/100‑1 (MQ=255)
                       attattCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTcc                                                                      <  2:1511301/100‑1 (MQ=255)
                        ttattCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCg                                                                     <  1:1105694/100‑1 (MQ=255)
                        ttattCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCg                                                                     >  2:4493858/1‑100 (MQ=255)
                           ttCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGtat                                                                  >  2:4630341/1‑100 (MQ=255)
                           tggccATCCGCGTCTGTTCTGGCTAAGGCAGGGGCGATTTGGGCGGCGCCATATTGTCGATGGTTTCAGCGTTGAGTTAAGCAAAGTCGTTCGTCCGtat                                                                  <  2:3689422/97‑1 (MQ=255)
                             cccATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTatat                                                                >  2:2710360/1‑100 (MQ=255)
                             cccATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTatat                                                                >  1:2136569/1‑100 (MQ=255)
                              ccATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATCTTGTCGATGGTTTCAGTGTTGAGTTAAGCACAGTCGTTCGTCCGTGTAtt                                                               >  2:4627861/1‑100 (MQ=255)
                              ccATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATAtt                                                               >  1:4026152/1‑100 (MQ=255)
                                aTCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCg                                                             <  2:71525/100‑1 (MQ=255)
                                 tCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGt                                                            <  1:2658719/100‑1 (MQ=255)
                                 tCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGt                                                            >  1:3383404/1‑100 (MQ=255)
                                  ccGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTg                                                           <  2:3493325/100‑1 (MQ=255)
                                  ccGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTg                                                           <  2:1070252/100‑1 (MQ=255)
                                  ccGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTg                                                           <  2:903042/100‑1 (MQ=255)
                                  ccGCGTCCGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGAGGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTg                                                           <  2:5112721/100‑1 (MQ=255)
                                    gcgTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAg                                                         <  1:1486588/100‑1 (MQ=255)
                                    gcgTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAg                                                         <  2:461329/100‑1 (MQ=255)
                                       tCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAgcgc                                                      <  2:5286441/100‑1 (MQ=255)
                                       tCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGCCGTTCGTCCGTATATTCGTGAgcgc                                                      <  1:3446665/100‑1 (MQ=255)
                                          gTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgtt                                                   >  1:1061290/1‑100 (MQ=255)
                                          gTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgtt                                                   >  2:285781/1‑100 (MQ=255)
                                          gTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgtt                                                   >  2:523175/1‑100 (MQ=255)
                                            tCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgttgt                                                 <  2:4208432/100‑1 (MQ=255)
                                              tGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGttgttg                                               <  2:1941135/100‑1 (MQ=255)
                                               ggCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGa                                              >  2:3516066/1‑100 (MQ=255)
                                               ggCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGa                                              >  1:5411333/1‑100 (MQ=255)
                                               ggCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGa                                              >  2:3089807/1‑100 (MQ=255)
                                               ggCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGa                                              >  2:2834173/1‑100 (MQ=255)
                                                gCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAc                                             >  1:3229278/1‑100 (MQ=255)
                                                 cTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc                                            <  1:950067/100‑1 (MQ=255)
                                                  tAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCa                                           <  1:2644814/100‑1 (MQ=255)
                                                    aGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGc                                         <  1:5136910/100‑1 (MQ=255)
                                                      tCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTg                                       <  1:3392831/100‑1 (MQ=255)
                                                       cAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTgg                                      >  2:1714557/1‑100 (MQ=255)
                                                        aGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGgc                                     <  2:305648/100‑1 (MQ=255)
                                                        aGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGgc                                     <  1:5281525/100‑1 (MQ=255)
                                                         gACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGgcg                                    >  2:1198239/1‑100 (MQ=255)
                                                           cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCa                                  <  1:3831039/100‑1 (MQ=255)
                                                            gcaaTTTGAGCAGCGCCATACTGGCGATGGTTTCAGTGTTGAGTTAAGCTAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCGGCTGGCGCat                                 <  2:1080569/97‑1 (MQ=255)
                                                              cATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCatat                               <  1:3255827/100‑1 (MQ=255)
                                                              cATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCatat                               >  2:24260/1‑100 (MQ=255)
                                                              cATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCatat                               >  2:2017758/1‑100 (MQ=255)
                                                               aTTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATAtt                              <  2:3323026/100‑1 (MQ=255)
                                                               aTTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATAtt                              <  2:1455576/100‑1 (MQ=255)
                                                                 ttGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGa                            >  2:2739301/1‑100 (MQ=255)
                                                                  tGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGAGTTGTTGGCCAGCTGGCGCATATTGAt                           >  2:4920486/1‑100 (MQ=255)
                                                                   gAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAATCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtc                          <  1:4923835/100‑1 (MQ=255)
                                                                   gAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtc                          <  2:5334700/100‑1 (MQ=255)
                                                                   gAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtc                          >  2:1818703/1‑100 (MQ=255)
                                                                    agcagcGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtct                         >  2:5387210/1‑100 (MQ=255)
                                                                      cagcGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCa                       <  1:2334328/100‑1 (MQ=255)
                                                                       agcGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCAc                      <  2:368365/100‑1 (MQ=255)
                                                                       agcGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCAc                      <  1:24466/100‑1 (MQ=255)
                                                                        gcgcCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCAct                     >  2:4265102/1‑100 (MQ=255)
                                                                            cATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTgg                 <  2:427363/100‑1 (MQ=255)
                                                                            cATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTgg                 <  1:4265872/100‑1 (MQ=255)
                                                                              tatTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGcc               >  2:1872402/1‑100 (MQ=255)
                                                                              tatTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGcc               >  1:775002/1‑100 (MQ=255)
                                                                                ttGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa             >  2:5058276/1‑100 (MQ=255)
                                                                                   tCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGc          <  1:3630823/100‑1 (MQ=255)
                                                                                    cGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCg         <  1:4409372/100‑1 (MQ=255)
                                                                                    cGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCg         >  2:3086658/1‑100 (MQ=255)
                                                                                     gATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCgg        >  2:3124761/1‑100 (MQ=255)
                                                                                     gATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCATATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCgg        <  2:4138739/100‑1 (MQ=255)
                                                                                      aTGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGt       <  1:917667/100‑1 (MQ=255)
                                                                                        ggTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTgg     <  2:4292997/100‑1 (MQ=255)
                                                                                          tttCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCg   <  2:2844214/100‑1 (MQ=255)
                                                                                          tttCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCg   <  2:1810638/100‑1 (MQ=255)
                                                                                          tttCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCg   >  1:2933976/1‑100 (MQ=255)
                                                                                          tttCAGTGTGGAGTGAAGCAAAGTCGTTCGTTCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCg   <  2:2844681/100‑1 (MQ=255)
                                                                                           ttCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCGa  >  1:1557391/1‑100 (MQ=255)
                                                                                                 |                                                                                             
GCGCAGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCGA  >  NC_000913/1815354‑1815544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: