BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,809	T→G	K384T (AAA→ACA)	yaaJ ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,809	0	T	G	99.3%	496.8 / ‑5.7	143	K384T (AAA→ACA)	yaaJ	putative transporter
Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (73/68); total (74/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 143 total aligned reads displayed.

ATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAA  >  NC_000913/6713‑6905

 ttATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAgg                                                                                              >  1:912433/1‑100 (MQ=255)

 ttATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAgg                                                                                              <  2:2552176/100‑1 (MQ=255)

 ttATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAgg                                                                                              <  2:1172315/100‑1 (MQ=255)

    tGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGgtt                                                                                           >  2:2252163/1‑100 (MQ=255)

     gATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGgttg                                                                                          >  1:650386/1‑100 (MQ=255)

      atatCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGgttgt                                                                                         >  1:3044945/1‑100 (MQ=255)

       tatCTGCCAGTTGCCACATCAGCGGAAGGCGTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTATGgttgtt                                                                                        <  2:1830409/100‑1 (MQ=255)

        atCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACTGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTc                                                                                       >  1:1043733/1‑100 (MQ=255)

        atCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTc                                                                                       <  1:42307/100‑1 (MQ=255)

         tCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCa                                                                                      <  2:279873/100‑1 (MQ=255)

         tCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCa                                                                                      >  1:3577786/1‑100 (MQ=255)

          cTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAg                                                                                     <  1:2729040/100‑1 (MQ=255)

           tGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAgg                                                                                    >  2:1322458/1‑100 (MQ=255)

           tGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAgg                                                                                    >  1:387617/1‑100 (MQ=255)

            gCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGc                                                                                   <  1:272718/100‑1 (MQ=255)

                gTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTaa                                                                               >  2:3533996/1‑100 (MQ=255)

                  tGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTaaaa                                                                             >  1:326217/1‑100 (MQ=255)

                  tGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTaaaa                                                                             <  2:774855/100‑1 (MQ=255)

                   gCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTaaaaa                                                                            >  1:3338097/1‑100 (MQ=255)

                   gCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCACATCCGCAAACACCGGATCGCTGTAGGGTTGCTCAGGCGTaaaaa                                                                            <  2:1995754/100‑1 (MQ=255)

                    ccACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAaag                                                                           <  1:920873/100‑1 (MQ=255)

                    ccACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAaag                                                                           <  2:3648356/100‑1 (MQ=255)

                     cacaTCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAaaga                                                                          <  2:1103317/100‑1 (MQ=255)

                      acaTCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAaagaa                                                                         <  2:1389331/100‑1 (MQ=255)

                        aTCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAagaaga                                                                       <  2:1683977/100‑1 (MQ=255)

                        aTCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAagaaga                                                                       >  1:3173580/1‑100 (MQ=255)

                        aTCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAagaaga                                                                       >  1:1308327/1‑100 (MQ=255)

                        aTCAGCGGAAGGCTTAGCAAGGTGCCGCAGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAagaaga                                                                       <  1:1731113/100‑1 (MQ=255)

                         tCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAagag                                                                      >  1:2668031/1‑100 (MQ=255)

                         tCAGCGGAACGCTTAGCAAGGTGACGCCGATGACCGTTGCGACGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAagag                                                                      <  1:3557432/100‑1 (MQ=255)

                          cAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGTAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAgaga                                                                     >  1:1287835/1‑100 (MQ=255)

                           aGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGAt                                                                    <  1:44500/100‑1 (MQ=255)

                           aGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGAt                                                                    >  2:2435861/1‑100 (MQ=255)

                            gCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGAtt                                                                   >  1:1787532/1‑100 (MQ=255)

                               gAAGGCTTAGCAAGCTGCCGCCGATGACCGTTGCGAAGGTGCACATCCACAAACACCAGCTCGCTGTAGGGTTGCTAGGGCGTGCACAGAAGAGAATggt                                                                >  2:526457/1‑98 (MQ=255)

                                aaGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGttt                                                               <  1:2153025/100‑1 (MQ=255)

                                aaGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGttt                                                               >  1:2125593/1‑100 (MQ=255)

                                  ggCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTc                                                             >  2:224520/1‑100 (MQ=255)

                                    tctAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCgg                                                           <  2:1203150/98‑1 (MQ=255)

                                    cTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCgg                                                           <  2:1519645/100‑1 (MQ=255)

                                     ttAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGc                                                          <  1:3551613/100‑1 (MQ=255)

                                     ttAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGc                                                          <  1:1113740/100‑1 (MQ=255)

                                      tAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCa                                                         >  1:2069675/1‑100 (MQ=255)

                                       aGCAAGGTGCCGCCGATGACCGTTGCGAATGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCAt                                                        <  1:3728159/100‑1 (MQ=255)

                                       aGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCggaga                                                        >  1:1226293/1‑97 (MQ=255)

                                       aGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCAt                                                        >  2:3496689/1‑100 (MQ=255)

                                        gCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATa                                                       <  1:1961500/100‑1 (MQ=255)

                                        gCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATa                                                       >  1:2634747/1‑100 (MQ=255)

                                          aaGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATaaa                                                     >  1:1417872/1‑100 (MQ=255)

                                            ggTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATg                                                   <  1:2146253/100‑1 (MQ=255)

                                            ggTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATg                                                   >  1:2453951/1‑100 (MQ=255)

                                               gccgccGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAg                                                >  2:2427110/1‑100 (MQ=255)

                                               gccgccGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAg                                                <  2:1152490/100‑1 (MQ=255)

                                                ccgccgATTACCGTTCCGAACGCGCAGATCCCCAAACACCAGATCGCTCTAAGCTAATGCAGACGGAAAAAGAAAAGGTTTTTTTCGGCATAAATGTatt                                               <  2:368726/100‑3 (MQ=255)

                                                ccgccgATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATGAATGTAGt                                               >  2:2524764/1‑100 (MQ=255)

                                                  gccgATGGCCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTg                                             <  2:3070029/100‑1 (MQ=255)

                                                  gccgATGACCGTTGCGAAGTTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGCTCAGGCGTAAAAAGAAAAGATGGTTTTCGGCATAAATGTAGtta                                             <  2:320390/100‑2 (MQ=255)

                                                   ccgATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTgg                                            <  2:2137601/100‑1 (MQ=255)

                                                    cgATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGc                                           <  1:3330626/100‑1 (MQ=255)

                                                    cgATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGc                                           <  1:2886540/100‑1 (MQ=255)

                                                     gATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCa                                          >  2:1099217/1‑100 (MQ=255)

                                                        gACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACg                                       <  1:2770206/100‑1 (MQ=255)

                                                          ccGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGAt                                     <  1:1038697/100‑1 (MQ=255)

                                                          ccGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGAt                                     >  1:2297432/1‑100 (MQ=255)

                                                          ccGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGAt                                     >  1:1463742/1‑100 (MQ=255)

                                                          ccGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGAt                                     <  1:3998943/100‑1 (MQ=255)

                                                          ccGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGAt                                     >  1:86109/1‑100 (MQ=255)

                                                           cGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATg                                    <  2:669901/100‑1 (MQ=255)

                                                             ttGCGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTGAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGa                                  <  2:3686700/100‑1 (MQ=255)

                                                                cGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCt                               >  2:2159874/1‑100 (MQ=255)

                                                                cGAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCt                               >  1:53370/1‑100 (MQ=255)

                                                                 gAAGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTg                              >  1:2305531/1‑100 (MQ=255)

                                                                   aGGTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGGTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGaa                            >  1:1216588/1‑100 (MQ=255)

                                                                   aGGTGCAGATCCGCAAACACCAGATCGCTGTACGGTTGCTCAGGCGAAAAAAGAAGAGGTTGTTTTCGGCATAAATGTAGCTGGCAACGATGGAGCTGaa                            >  1:3621563/1‑100 (MQ=255)

                                                                    ggTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAg                           >  1:829167/1‑100 (MQ=255)

                                                                    ggTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAg                           <  1:2367733/100‑1 (MQ=255)

                                                                    ggTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAg                           >  2:358446/1‑100 (MQ=255)

                                                                    ggTGCAGATCCGCAAACACCAGATCGCTGTAGAGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAg                           <  2:1671944/100‑1 (MQ=255)

                                                                     gTGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAgg                          <  2:2589930/100‑1 (MQ=255)

                                                                      tGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGc                         >  1:633856/1‑100 (MQ=255)

                                                                      tGCAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGc                         >  1:2282513/1‑100 (MQ=255)

                                                                        cAGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCaa                       >  1:2424187/1‑100 (MQ=255)

                                                                         aGATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCaaa                      <  1:883162/100‑1 (MQ=255)

                                                                          gATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAAc                     <  2:3734933/100‑1 (MQ=255)

                                                                          gATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAAc                     <  2:3734914/100‑1 (MQ=255)

                                                                          gATCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTCTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAAc                     <  1:3473942/100‑1 (MQ=255)

                                                                            tCCGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAg                   <  2:2667104/100‑1 (MQ=255)

                                                                             ccGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGa                  >  1:370536/1‑100 (MQ=255)

                                                                              cGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGaa                 >  2:270620/1‑100 (MQ=255)

                                                                              cGCAAACACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGaa                 >  1:1548702/1‑100 (MQ=255)

                                                                                 aaaCACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATaa              <  2:2060584/100‑1 (MQ=255)

                                                                                  aaCACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAAc             >  2:382126/1‑100 (MQ=255)

                                                                                  aaCACCAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGAAGCGGAAGGCAAACAGAATAAc             <  2:2811525/100‑1 (MQ=255)

                                                                                      ccAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACcaca         <  1:2465023/100‑1 (MQ=255)

                                                                                      ccAGATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACcaca         >  2:1480449/1‑100 (MQ=255)

                                                                                         gATCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAgg      >  2:3746667/1‑100 (MQ=255)

                                                                                          aTCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAggg     <  2:2247590/100‑1 (MQ=255)

                                                                                          aTCGCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAggg     <  2:3967537/100‑1 (MQ=255)

                                                                                             gCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTaa  >  2:3420474/1‑100 (MQ=255)

                                                                                             gCTGTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTaa  >  2:514623/1‑100 (MQ=255)

ATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAA  >  NC_000913/6713‑6905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`