Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,113,790 T→G K180Q (AAA→CAA)  wcaN ← putative regulatory subunit for GalU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,113,7900TG99.0% 339.8 / NA 99K180Q (AAA→CAA) wcaNputative regulatory subunit for GalU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (48/50);  minor base C (0/1);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGCAATCATGGCA  >  NC_000913/2113693‑2113884
                                                                                                 |                                                                                              
aCGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAg                                                                                              <  1:3132229/100‑1 (MQ=255)
  ggCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTc                                                                                            <  1:3352704/100‑1 (MQ=255)
      atgatgTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGa                                                                                        <  1:2435477/100‑1 (MQ=255)
      atgatgTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGa                                                                                        >  1:2071992/1‑100 (MQ=255)
      atgatgTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGa                                                                                        >  2:3162845/1‑100 (MQ=255)
       tgatgTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTTTTTTTTTTTTACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGAt                                                                                       >  2:3440193/1‑100 (MQ=255)
       tgatgTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGAt                                                                                       <  2:795311/100‑1 (MQ=255)
       tgatgTCTGAGTCCAGCCTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTAACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGAt                                                                                       <  2:164384/100‑1 (MQ=255)
        gatgTCTGAGTCCAGCGTGTGCGGGTGATCGGGTGTTTCGGTAAATTCAACAATGCGGCTGACTTTACCCGCACGGTCCAGCGGATCTTGAGTCTGGATg                                                                                      <  2:3896108/100‑1 (MQ=255)
            tCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACgg                                                                                  <  1:2406117/100‑1 (MQ=255)
            tCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACgg                                                                                  <  1:1388312/100‑1 (MQ=255)
              tGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAg                                                                                >  1:2048461/1‑100 (MQ=255)
              tGAGTCCAGCGTCTGCGGCTGATCCGGCGTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAg                                                                                <  1:228324/100‑1 (MQ=255)
               gAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGt                                                                               >  1:1767741/1‑100 (MQ=255)
               gAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGt                                                                               >  2:19807/1‑100 (MQ=255)
                 gTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTAt                                                                             >  1:4049366/1‑100 (MQ=255)
                   ccAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTTTTc                                                                           >  2:1167448/1‑100 (MQ=255)
                    cAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCa                                                                          <  2:1796761/100‑1 (MQ=255)
                    cAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCa                                                                          >  2:7173/1‑100 (MQ=255)
                     aGCGTCTGCGGCTGATCCGGTTTTTTTTTTATTTTCACAATGCGGCTGACTTTACCCTCACGCTCCTGCCGCTCTTGCGTCCGGATGCCGGTCTATTCag                                                                         >  2:1671028/1‑100 (MQ=255)
                     aGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCag                                                                         >  2:2421839/1‑100 (MQ=255)
                       cGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCagag                                                                       <  1:122656/100‑1 (MQ=255)
                        gTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCagaga                                                                      <  2:1624776/100‑1 (MQ=255)
                        gTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCagaga                                                                      >  1:1122233/1‑100 (MQ=255)
                        gTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTCGAGTCTGGATGACGGAGTATTCagaga                                                                      <  1:2566515/100‑1 (MQ=255)
                            gCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTc                                                                  >  1:3999988/1‑100 (MQ=255)
                             cGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGGCa                                                                 >  1:3265018/1‑100 (MQ=255)
                              ggCTGATCCGGTTTTTCGATAAATTCAACAATGGGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCAc                                                                >  1:271572/1‑100 (MQ=255)
                              ggCTGATCCGGTTTTTCGATAAATTAAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTGAGAGAGGTCAc                                                                >  2:3194248/1‑100 (MQ=255)
                               gCTGATCCGGTTTTTTTATAAATTTAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCAcc                                                               >  2:1032048/1‑100 (MQ=255)
                                cTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTCTTCAGAGCGGTCAccc                                                              >  2:2417006/1‑100 (MQ=255)
                                  gATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCgg                                                            >  1:3860485/1‑100 (MQ=255)
                                   aTCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGAAGGAGTATTCAGAGCGGTCACCCGGc                                                           >  1:3583930/1‑100 (MQ=255)
                                    tCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCa                                                          <  2:2276633/100‑1 (MQ=255)
                                    tCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCa                                                          >  1:3936177/1‑100 (MQ=255)
                                      cGGTTTTTCGATAAATTCAACATTGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATa                                                        >  1:3413830/1‑100 (MQ=255)
                                      cGGTTTCTCTATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATa                                                        <  2:2364149/100‑1 (MQ=255)
                                         tttttCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGt                                                     >  2:3939918/1‑100 (MQ=255)
                                          ttttCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCCCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGtt                                                    >  1:270433/1‑100 (MQ=255)
                                            ttCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGtttt                                                  <  2:2384572/100‑1 (MQ=255)
                                             tCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTg                                                 <  2:3893535/100‑1 (MQ=255)
                                              cGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGc                                                >  1:1774766/1‑100 (MQ=255)
                                               gATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGcc                                               <  2:1528832/100‑1 (MQ=255)
                                               gATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGcc                                               >  2:2128945/1‑100 (MQ=255)
                                                 tAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAg                                             <  1:1609498/100‑1 (MQ=255)
                                                 tAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAg                                             <  1:3425774/100‑1 (MQ=255)
                                                 tAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAg                                             <  2:3209069/100‑1 (MQ=255)
                                                    aTTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCAc                                          >  2:656815/1‑100 (MQ=255)
                                                    aTTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCAc                                          >  1:1150197/1‑100 (MQ=255)
                                                    aTTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCAc                                          >  1:1269236/1‑100 (MQ=255)
                                                    aTTCAACAATGCGGCTGACTTTACCCACACGGTCCAGCGGATCTTGAGACTGGATGACGGAGTATTCAGAGAGGTAACCCGGCATACGTTTTGCCAGCAc                                          >  2:1296199/1‑100 (MQ=255)
                                                     ttCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCAcc                                         <  2:2039758/100‑1 (MQ=255)
                                                     ttCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCAcc                                         >  1:1810875/1‑100 (MQ=255)
                                                     ttCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCAcc                                         <  1:177882/100‑1 (MQ=255)
                                                      tCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCt                                        >  2:1945944/1‑100 (MQ=255)
                                                       caacaaTGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTg                                       <  1:1773933/100‑1 (MQ=255)
                                                         acaaTGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTggn                                     <  1:3244607/100‑2 (MQ=255)
                                                          caaTGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCt                                    >  2:1294154/1‑100 (MQ=255)
                                                            aTGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGc                                  <  1:3539429/100‑1 (MQ=255)
                                                             tGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCg                                 >  2:443483/1‑100 (MQ=255)
                                                             tGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCg                                 <  2:2488956/100‑1 (MQ=255)
                                                             tGCGGCTGACTTTACCCTCACGGTCCAGCGGCGCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCg                                 <  1:695295/100‑1 (MQ=255)
                                                              gCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCgg                                >  1:1962966/1‑100 (MQ=255)
                                                                ggCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGcc                              >  1:534801/1‑100 (MQ=255)
                                                                ggCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGcc                              >  1:1977426/1‑100 (MQ=255)
                                                                ggCTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGcc                              <  1:3807327/100‑1 (MQ=255)
                                                                  cTGACTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCg                            <  1:2631099/100‑1 (MQ=255)
                                                                      cTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTc                        <  2:3742259/100‑1 (MQ=255)
                                                                      cTTTACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTc                        <  2:2874300/100‑1 (MQ=255)
                                                                        ttACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGt                      <  2:3467102/100‑1 (MQ=255)
                                                                         tACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGtt                     <  1:846864/100‑1 (MQ=255)
                                                                         tACCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGtt                     >  1:95518/1‑100 (MQ=255)
                                                                         tACCCTCACGGTCCAGCGGCTCTTCAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCCCCATACGTTTTGCCAGCACCTGGCTGCTGCCCGTTTCGtt                     <  1:847109/100‑1 (MQ=255)
                                                                          aCCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTg                    <  2:1099961/100‑1 (MQ=255)
                                                                          aCCCTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTg                    <  2:1587085/100‑1 (MQ=255)
                                                                           cccTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGa                   >  1:2034465/1‑100 (MQ=255)
                                                                           cccTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGa                   >  2:2649308/1‑100 (MQ=255)
                                                                            ccTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGaa                  <  1:1318166/100‑1 (MQ=255)
                                                                            ccTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGaa                  <  2:1620828/100‑1 (MQ=255)
                                                                             cTCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGaaa                 <  1:672475/100‑1 (MQ=255)
                                                                              tCACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAAc                <  1:1414359/100‑1 (MQ=255)
                                                                               naCGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACg               >  1:3245132/2‑100 (MQ=255)
                                                                               cACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACg               <  2:2229162/100‑1 (MQ=255)
                                                                               cACGGTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACg               <  1:578814/100‑1 (MQ=255)
                                                                                  ggTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGc            <  2:3691721/100‑1 (MQ=255)
                                                                                  ggTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGc            >  1:1184322/1‑100 (MQ=255)
                                                                                  ggTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGc            <  1:545496/100‑1 (MQ=255)
                                                                                  ggTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGc            >  1:239445/1‑100 (MQ=255)
                                                                                  ggTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGc            >  2:2129094/1‑100 (MQ=255)
                                                                                  ggTCCAGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGc            <  1:510235/100‑1 (MQ=255)
                                                                                       aGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGCAATCa       >  2:481882/1‑100 (MQ=255)
                                                                                       aGCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGCAATCa       >  1:3286396/1‑100 (MQ=255)
                                                                                        gCGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGCAATCAt      <  1:2653791/100‑1 (MQ=255)
                                                                                         cGGCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGCAATCATg     <  2:524255/100‑1 (MQ=255)
                                                                                           gCTCTTGAGTCTGGATGAGGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCTTTTCGTTGAAACGTGCAATCATGgc   <  2:2848269/100‑1 (MQ=255)
                                                                                           gCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGCAATCATGgc   >  2:3426144/1‑100 (MQ=255)
                                                                                           gCTCTTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGCAATCATGgc   <  1:2311677/100‑1 (MQ=255)
                                                                                            ctctTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGCAATCATGgca  <  1:1712299/100‑1 (MQ=255)
                                                                                            ctctTGAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGCAATCATGgca  >  2:1881561/1‑100 (MQ=255)
                                                                                                 |                                                                                              
ACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTGCGGCCCGTTTCGTTGAAACGTGCAATCATGGCA  >  NC_000913/2113693‑2113884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: