Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,152,171 T→G E101A (GAA→GCA)  yegK ← ser/thr phosphatase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,152,1710TG92.4% 429.0 / 16.0 144E101A (GAA→GCA) yegKser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base T (5/6);  new base G (72/61);  total (77/67)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

CCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGAC  >  NC_000913/2152075‑2152266
                                                                                                |                                                                                               
ccgccATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGCACCGCTAATTCTTTGGCTGCCg                                                                                              >  1:283633/1‑100 (MQ=255)
ccgccATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGTCTGcct                                                                                              >  1:2041484/1‑99 (MQ=255)
ccgccATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCg                                                                                              >  2:776718/1‑100 (MQ=255)
  gccATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTgccgcc                                                                                            >  2:743750/1‑100 (MQ=255)
  gccATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTgccgcc                                                                                            <  1:2044106/100‑1 (MQ=255)
   ccATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCt                                                                                           <  1:3661514/100‑1 (MQ=255)
    cATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTc                                                                                          <  2:593146/100‑1 (MQ=255)
     aTCGCCAATCTGCATAAGCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGTCTCg                                                                                         <  2:2714359/100‑1 (MQ=255)
      tCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCgg                                                                                        <  2:1452355/100‑1 (MQ=255)
      tCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCgg                                                                                        <  1:4074860/100‑1 (MQ=255)
       cGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGc                                                                                       >  2:3635259/1‑100 (MQ=255)
        gCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCa                                                                                      >  1:1705427/1‑100 (MQ=255)
         ccAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCaa                                                                                     >  2:1222552/1‑100 (MQ=255)
         ccAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCaa                                                                                     >  1:3304755/1‑100 (MQ=255)
            aTCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCaaaaa                                                                                  <  1:449096/100‑1 (MQ=255)
               tGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTc                                                                               >  1:1133467/1‑100 (MQ=255)
               tGCATAATCAAGGTGCCGTCAGGCGATGAAACCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTc                                                                               >  2:3971837/1‑100 (MQ=255)
               tGCATAATCAAGGTGCCGTCAGGAGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTc                                                                               >  2:1773283/1‑100 (MQ=255)
                gCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGGAAAAAGGCg                                                                              >  2:3458516/1‑100 (MQ=255)
                gCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCg                                                                              >  2:174747/1‑100 (MQ=255)
                gCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCCCGGCCAAACGTCg                                                                              >  2:1176074/1‑100 (MQ=255)
                 cATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGt                                                                             <  2:531539/100‑1 (MQ=255)
                 cATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGt                                                                             >  2:1416775/1‑100 (MQ=255)
                 cATAATCAAGGGGCCCCCAGGCGATCAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTTTCTGGCTGCCGCCTCGGCAAAAAGTCGt                                                                             <  2:1709046/100‑1 (MQ=255)
                 cATAATCAAGGCAGCGGCAGGCGACGAAATCAGTCCCAGATTTGTTCAGGCAAAATCGCGAACCTCTAAGTCTTTGGCTGCCGCCTCGGCAAAAGGTCgn                                                                             <  2:3595652/100‑2 (MQ=255)
                    aaTCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGa                                                                          >  1:1062421/1‑100 (MQ=255)
                    aaTCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCGAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGa                                                                          <  1:2827273/100‑1 (MQ=255)
                     ntCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGAc                                                                         >  1:3254434/2‑100 (MQ=255)
                     aTCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGAc                                                                         <  1:1222169/100‑1 (MQ=255)
                     aTCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGAc                                                                         <  1:1523521/100‑1 (MQ=255)
                     aTCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCCAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGAc                                                                         >  2:3747011/1‑100 (MQ=255)
                         aGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAAt                                                                     >  1:2218318/1‑100 (MQ=255)
                           gTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATgg                                                                   >  2:1335354/1‑100 (MQ=255)
                            tGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGt                                                                  >  2:3122712/1‑100 (MQ=255)
                             gCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCTCCTCGGCAAAAAGTCGTTGACGAATGGTc                                                                 <  1:630092/100‑1 (MQ=255)
                             gCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTc                                                                 <  1:153956/100‑1 (MQ=255)
                             gCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTc                                                                 >  1:1698048/1‑100 (MQ=255)
                              ccGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCa                                                                <  1:3054923/100‑1 (MQ=255)
                               cGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAg                                                               <  1:1855197/100‑1 (MQ=255)
                               cGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAg                                                               >  2:2004200/1‑100 (MQ=255)
                               cGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAg                                                               <  2:2353203/100‑1 (MQ=255)
                               cGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGCCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAg                                                               <  1:2035286/100‑1 (MQ=255)
                                   aGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTAcc                                                           <  2:2557006/100‑1 (MQ=255)
                                   aGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTAcc                                                           >  2:2716526/1‑100 (MQ=255)
                                     gCGATGAAATCAGTCCCAGAAATGTCCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCat                                                         <  2:1611371/100‑1 (MQ=255)
                                      cGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCata                                                        >  1:3044597/1‑100 (MQ=255)
                                       gATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCatat                                                       >  2:1754246/1‑100 (MQ=255)
                                        aTGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATAtt                                                      >  1:3573560/1‑100 (MQ=255)
                                        aTGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATAtt                                                      <  1:2544103/100‑1 (MQ=255)
                                         tGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATAttt                                                     >  1:3552004/1‑100 (MQ=255)
                                           aaaTCAGTGACAGAAATGAGCAGGCAAAATCGCGAACCGCTAATTTTTTGGCTGAAGAAAAGGAAAAAAGTCGTTGACGAATGGTCAGTACCATATTtgt                                                   <  2:2838681/100‑1 (MQ=255)
                                           aaaTCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTtgt                                                   >  2:2277621/1‑100 (MQ=255)
                                           aaaTCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTtgt                                                   <  2:2184291/100‑1 (MQ=255)
                                            aaTCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTtgtg                                                  <  2:3564690/100‑1 (MQ=255)
                                               cAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCg                                               <  2:1673577/100‑1 (MQ=255)
                                               cAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCg                                               <  2:1642929/100‑1 (MQ=255)
                                               cAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCg                                               <  2:631939/100‑1 (MQ=255)
                                                 gTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAg                                             >  2:1941235/1‑100 (MQ=255)
                                                  tCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAgg                                            <  1:3189650/100‑1 (MQ=255)
                                                  tCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAgg                                            >  2:3681087/1‑100 (MQ=255)
                                                   cccAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGa                                           >  1:2083571/1‑100 (MQ=255)
                                                   cccAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGa                                           >  1:305824/1‑100 (MQ=255)
                                                    ccAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCATTACCATATTTGTGGCGAGGAc                                          >  2:618261/1‑100 (MQ=255)
                                                    ccAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGAc                                          >  2:2898668/1‑100 (MQ=255)
                                                    ccAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGAc                                          <  1:255946/100‑1 (MQ=255)
                                                     cAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGAca                                         <  1:1186846/100‑1 (MQ=255)
                                                     cAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGAca                                         >  1:3909062/1‑100 (MQ=255)
                                                           tgtgCAGGCAAACTCACGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTa                                   <  2:3424060/100‑1 (MQ=255)
                                                           tgtgCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGCCGAATGGTCAGTACCATATTTGTGGCGAGGGCATCAGTa                                   >  2:1254231/1‑100 (MQ=255)
                                                           tgtgCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTa                                   <  1:725407/100‑1 (MQ=255)
                                                           tgtgCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTa                                   >  2:280192/1‑100 (MQ=255)
                                                            gtgCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTaa                                  >  1:695676/1‑100 (MQ=255)
                                                              gCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGc                                <  2:1189053/100‑1 (MQ=255)
                                                              gCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGc                                <  1:3229519/100‑1 (MQ=255)
                                                               cAGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGcc                               >  1:871688/1‑100 (MQ=255)
                                                                aGGCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGCTCCGTACCATATTTGTGGCGAGGACATCATTAAGccc                              >  2:2477778/1‑100 (MQ=255)
                                                                 ggCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGcccc                             >  1:1950390/1‑100 (MQ=255)
                                                                 ggCAAAATCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGcccc                             <  1:985939/100‑1 (MQ=255)
                                                                    aaaaTCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAAt                          <  1:347094/100‑1 (MQ=255)
                                                                      aaTCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTc                        <  1:2581805/100‑1 (MQ=255)
                                                                       aTCGCGAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTcc                       >  1:2318444/1‑100 (MQ=255)
                                                                         cgcgAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTcccc                     <  1:1442060/100‑1 (MQ=255)
                                                                         cgcgAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTcccc                     <  1:2374365/100‑1 (MQ=255)
                                                                          gcgAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCg                    >  2:3521404/1‑100 (MQ=255)
                                                                          gcgAACCGCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCg                    >  1:76103/1‑100 (MQ=255)
                                                                              aCCGCTAATTCTTTGGCTTCCGCCTCGTCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCt                <  2:2788257/100‑1 (MQ=255)
                                                                                 gCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGACGACTGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCa             >  1:3472582/1‑100 (MQ=255)
                                                                                 gCTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCa             >  2:2808136/1‑100 (MQ=255)
                                                                                  cTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCAc            >  1:372605/1‑100 (MQ=255)
                                                                                  cTAATTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCAc            <  2:3999576/100‑1 (MQ=255)
                                                                                    aaTTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACtt          <  2:3743286/100‑1 (MQ=255)
                                                                                     aTTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACttt         >  1:3432357/1‑100 (MQ=255)
                                                                                     aTTCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACttt         >  2:3114878/1‑100 (MQ=255)
                                                                                       tCTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACttttt       >  2:2598040/1‑100 (MQ=255)
                                                                                        cTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTg      <  2:1668113/100‑1 (MQ=255)
                                                                                        cTTTGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTg      <  2:1497051/100‑1 (MQ=255)
                                                                                         tttGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGc     <  1:2565678/100‑1 (MQ=255)
                                                                                          ttGGCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCg    <  1:3440199/100‑1 (MQ=255)
                                                                                            ggCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGAc  >  2:2367149/1‑100 (MQ=255)
                                                                                            ggCTGCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGAc  <  2:1523538/100‑1 (MQ=255)
                                                                                                |                                                                                               
CCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGAC  >  NC_000913/2152075‑2152266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: