Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,164,104 A→C Y410S (TAT→TCT)  baeS → sensory histidine kinase in two‑component regulatory system with BaeR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,164,1040AC93.8% 448.6 / 12.3 146Y410S (TAT→TCT) baeSsensory histidine kinase in two‑component regulatory system with BaeR
Reads supporting (aligned to +/- strand):  ref base A (5/4);  new base C (70/66);  total (76/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

TCTGCCGGGCAGCGCGACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTATCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTCGCATTAT  >  NC_000913/2164007‑2164200
                                                                                                 |                                                                                                
tctGCCGGGCAGCGCGACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTtctc                                                                                                <  1:2814385/100‑1 (MQ=255)
 ctGCCGGGCAGCGCGACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCg                                                                                               >  1:670972/1‑100 (MQ=255)
 ctGCCGGGCAGCGCGACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCg                                                                                               >  1:641500/1‑100 (MQ=255)
 ctGCCGGGCAGCCCGACAAAACGCCGCGCATGAACTTTGCCGACAGTGCGCCAGGTGTCAGTGAAAAAAAAAAAAAAAAATTGTTTGAACGTTTTTCTCg                                                                                               <  2:2363835/100‑1 (MQ=255)
   gCCGGGCAGCGCGACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACTACCAGCTACAACAATTGTTTGAACGTTTTTCTCGCa                                                                                             >  1:3840654/1‑100 (MQ=255)
     cGGGCAGCGCGACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCAcc                                                                                           >  1:3548039/1‑100 (MQ=255)
         cAGCGCGACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAg                                                                                       <  1:2363465/100‑1 (MQ=255)
         cAGCGCGACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAg                                                                                       <  1:4022124/100‑1 (MQ=255)
           gcgcGACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGt                                                                                     <  1:3922872/100‑1 (MQ=255)
            cgccACAAGACGGTGCGCCAAACATTTGCCAAGATTGTGCCTGGTATTAGTGACAAAAAAAAAAAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGtt                                                                                    <  2:2468308/100‑1 (MQ=255)
              cgACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTcc                                                                                  <  1:3356193/100‑1 (MQ=255)
              cgACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTcc                                                                                  >  2:2650043/1‑100 (MQ=255)
                 cAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGc                                                                               >  2:123518/1‑100 (MQ=255)
                 cAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGc                                                                               >  1:894110/1‑100 (MQ=255)
                   aaaCGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCaa                                                                             >  1:1535780/1‑100 (MQ=255)
                      cGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCg                                                                          >  1:31064/1‑100 (MQ=255)
                       ggTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGt                                                                         >  2:1625801/1‑100 (MQ=255)
                       ggTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGt                                                                         <  1:2104494/100‑1 (MQ=255)
                        gTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTg                                                                        >  1:1866083/1‑100 (MQ=255)
                        gTGCGCCTGACATTTGCCGACAGTGCGACAGGTGTCAGTGACGATCAGCTACAAAAATTGCTTGAACGTTTTTCTCGCACCGAAGGCTCACGCAACCGTg                                                                        >  2:1777129/1‑100 (MQ=255)
                            gcCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAg                                                                    <  2:3026850/100‑1 (MQ=255)
                              cTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAgcg                                                                  <  2:914643/100‑1 (MQ=255)
                              cTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAgcg                                                                  <  1:3566394/100‑1 (MQ=255)
                              cTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAgcg                                                                  >  1:2351562/1‑100 (MQ=255)
                               tGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAgcgg                                                                 <  1:1152720/100‑1 (MQ=255)
                               tGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAgcgg                                                                 >  1:1647390/1‑100 (MQ=255)
                                  ccTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGcggcgg                                                              >  1:2326085/1‑100 (MQ=255)
                                  ccTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGcggcgg                                                              >  1:1897020/1‑100 (MQ=255)
                                   cTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGt                                                             >  1:2432068/1‑100 (MQ=255)
                                       gCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCg                                                         >  1:672546/1‑100 (MQ=255)
                                        ccGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCgg                                                        >  1:2533806/1‑100 (MQ=255)
                                        ccGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTATCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCgg                                                        >  1:3840752/1‑100 (MQ=255)
                                         cGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCggg                                                       >  1:1090788/1‑100 (MQ=255)
                                            cAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCgggctg                                                    <  1:1186351/100‑1 (MQ=255)
                                            cAGTGAGCCAGGTGTGAGTGACGATCAGCTACAAAAAGTGTTTGAACGTTTTTATCGCACCGAAGGTTCACGCAAACGTCCCCGCGGCAGTTCCaggctg                                                    >  2:3044714/1‑100 (MQ=255)
                                             aGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCgggctgg                                                   <  1:327226/100‑1 (MQ=255)
                                              gTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCgggctggg                                                  <  1:3695180/100‑1 (MQ=255)
                                              gTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCgggctggg                                                  >  1:1747327/1‑100 (MQ=255)
                                                gcgcCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGGAACCGTGCCAGCGGCGGATCCgggctggggc                                                >  2:3134310/1‑100 (MQ=255)
                                                gcgcCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCgggctggggc                                                <  2:3721906/100‑1 (MQ=255)
                                                gcgcCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCgggctggggc                                                <  2:2677889/100‑1 (MQ=255)
                                                gcgcCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCgggctggggc                                                <  1:2523219/100‑1 (MQ=255)
                                                  gcCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCgggctggggctg                                              >  1:1798084/1‑100 (MQ=255)
                                                  gcCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCgggctggggctg                                              >  2:687566/1‑100 (MQ=255)
                                                   ccAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGggctggggctgg                                             <  2:532777/100‑1 (MQ=255)
                                                   ccAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGggctggggctgg                                             <  1:1467429/100‑1 (MQ=255)
                                                   ccAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGggctggggctgg                                             <  1:1664634/100‑1 (MQ=255)
                                                   ccAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGggctggggctgg                                             >  1:2954577/1‑100 (MQ=255)
                                                    cAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGTGCTGGGGCTGGc                                            <  1:3516115/100‑1 (MQ=255)
                                                       gtgtCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGAt                                         >  2:2990870/1‑100 (MQ=255)
                                                       gtgtCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGAt                                         <  1:1746008/100‑1 (MQ=255)
                                                       gtgtCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGAt                                         <  2:1176467/100‑1 (MQ=255)
                                                       gtgtCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGAGGTTCCGCGCTGGGGCTGGCGAt                                         >  2:838480/1‑100 (MQ=255)
                                                       gtgtCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTACCGCAACCGTGCCAGCGGCGGTTCCGTGCTGGGGCTGGCGAt                                         >  2:2991022/1‑100 (MQ=255)
                                                       gtgtCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTATCTCTCCTCAGCTCCCCGCAACCGTCCCAGCGCCGCCTCCCGGCTGGGGCTCGCGAt                                         >  2:3338112/1‑100 (MQ=255)
                                                       gtgtCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTACTCGCACCGAAGGTTCCCGCAACCTTGCGAGCGGCGGTTCCGGGCTGGGGCTGGCGAt                                         <  1:3909643/100‑1 (MQ=255)
                                                         gtCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGAttt                                       <  1:2534836/100‑1 (MQ=255)
                                                         gtCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGAttt                                       >  1:483943/1‑100 (MQ=255)
                                                           cAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGc                                     <  2:2175107/100‑1 (MQ=255)
                                                            aGTGACGATCAGCTACGAGAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCTGCTGTTCCGGGCTGGGGCTGGCGATTTGcc                                    <  2:1172050/100‑1 (MQ=255)
                                                            aGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGcc                                    <  2:196417/100‑1 (MQ=255)
                                                            aGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGcc                                    <  1:3501764/100‑1 (MQ=255)
                                                            aGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTATCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGcc                                    >  1:214487/1‑100 (MQ=255)
                                                            aGTGACGATCAGCTACAAAAATTGTTTCAACGTTTTTCTCACACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCTGGCTGGGGCTGGCGATTTGcc                                    <  2:3825398/100‑1 (MQ=255)
                                                             gtcaCGATCAGCTACAAACATTGTTTCAACGTTTTTCTCGCACCGAAGCTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCt                                   >  2:107661/4‑100 (MQ=255)
                                                             gTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCt                                   >  2:1215304/1‑100 (MQ=255)
                                                             gTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCt                                   >  2:2889732/1‑100 (MQ=255)
                                                             gTGACGATCAGCTACAAAAATTGTTTGAACGTATTTCTCGCACCGAAGGTTCCCGCAACCGTGACAGCGTCGGTTCCGGGCTGGGGCTGGCGATTTGcat                                   >  2:3599337/1‑98 (MQ=255)
                                                              tGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTg                                  <  1:3120737/100‑1 (MQ=255)
                                                              tGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTg                                  <  1:1210447/100‑1 (MQ=255)
                                                              tGACGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTg                                  <  2:647223/100‑1 (MQ=255)
                                                                 cGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAAc                               >  1:1199614/1‑100 (MQ=255)
                                                                 cGATCAGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGACGATTTGCCTGAAc                               >  2:3652436/1‑100 (MQ=255)
                                                                      aGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACAttgt                          <  1:533156/100‑1 (MQ=255)
                                                                      aGCTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACAttgt                          <  1:2258933/100‑1 (MQ=255)
                                                                        cTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACAttgttg                        >  1:640699/1‑100 (MQ=255)
                                                                        cTACAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACAttgttg                        >  1:938602/1‑100 (MQ=255)
                                                                          aCAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGaa                      >  2:1332614/1‑100 (MQ=255)
                                                                           cAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAg                     <  2:1846107/100‑1 (MQ=255)
                                                                           cAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAg                     >  1:2022979/1‑100 (MQ=255)
                                                                           cAAAAATTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAg                     >  2:157511/1‑100 (MQ=255)
                                                                            aaaaaTTGTTTGAACGTTTTTCTCGCACCGAAGGTTTCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGc                    <  2:3549297/100‑1 (MQ=255)
                                                                            aaaaaTTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGc                    <  1:3902899/100‑1 (MQ=255)
                                                                            aaaaaTTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGc                    <  2:465835/100‑1 (MQ=255)
                                                                              aaaTTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGcac                  <  1:3657950/100‑1 (MQ=255)
                                                                               aaTTGTTTGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGcaca                 >  2:2275107/1‑100 (MQ=255)
                                                                                    tttGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGACCGCGGCGGTTCCGGGCTGGGGCTGGCGATTTAACTGAAAATTGTTGAAGCCCAtcctg            >  2:3018962/1‑96 (MQ=255)
                                                                                      tGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGt          >  1:1991132/1‑100 (MQ=255)
                                                                                      tGAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGt          >  2:559811/1‑100 (MQ=255)
                                                                                       gAACGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTc         >  2:297996/1‑100 (MQ=255)
                                                                                        aaCGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTcn        <  1:3249439/100‑2 (MQ=255)
                                                                                        aaCGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTCg        <  2:1718798/100‑1 (MQ=255)
                                                                                        aaCGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTCg        <  2:248736/100‑1 (MQ=255)
                                                                                        aaCGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTCg        <  1:3873837/100‑1 (MQ=255)
                                                                                         aCGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTCGc       >  1:990545/1‑100 (MQ=255)
                                                                                          cGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTCGCa      >  1:1119319/1‑100 (MQ=255)
                                                                                          cGTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTCGCa      <  1:584678/100‑1 (MQ=255)
                                                                                           gTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTCGCat     <  1:474486/100‑1 (MQ=255)
                                                                                           gTTTTTCTCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTCGCat     >  1:1167179/1‑100 (MQ=255)
                                                                                              tttATCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTCGCattat  <  2:1146030/100‑1 (MQ=255)
                                                                                                 |                                                                                                
TCTGCCGGGCAGCGCGACAAAACGGTGCGCCTGACCTTTGCCGACAGTGCGCCAGGTGTCAGTGACGATCAGCTACAAAAATTGTTTGAACGTTTTTATCGCACCGAAGGTTCCCGCAACCGTGCCAGCGGCGGTTCCGGGCTGGGGCTGGCGATTTGCCTGAACATTGTTGAAGCACATAATGGTCGCATTAT  >  NC_000913/2164007‑2164200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: