BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,181,901	A→C	D269A (GAC→GCC)	yegV →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,181,901	0	A	C	91.8%	447.7 / 27.3	159	D269A (GAC→GCC)	yegV	putative kinase
Reads supporting (aligned to +/- strand): ref base A (5/8); new base C (70/76); total (75/84)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

Only 100 of 159 total aligned reads displayed.

CGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCG  >  NC_000913/2181804‑2181996

cgtCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCa                                                                                               <  2:3841617/100‑1 (MQ=255)

 ntctcGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAg                                                                                              >  1:3967608/2‑100 (MQ=255)

 gtCTCGATAAAGAAGGCGCATGGTATTTCAGCGACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAg                                                                                              <  1:1111092/100‑1 (MQ=255)

 gtCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAg                                                                                              <  2:2815264/100‑1 (MQ=255)

 gtCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAg                                                                                              <  2:2709865/100‑1 (MQ=255)

 gtCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAg                                                                                              >  2:170205/1‑100 (MQ=255)

  tctcGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGt                                                                                             >  1:38554/1‑100 (MQ=255)

   ctcGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTc                                                                                            <  1:605352/100‑1 (MQ=255)

    tcGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCa                                                                                           <  2:69509/100‑1 (MQ=255)

    tcGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCa                                                                                           <  1:2152106/100‑1 (MQ=255)

     cGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCAt                                                                                          <  1:3225053/100‑1 (MQ=255)

     cGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCAt                                                                                          >  2:3036845/1‑100 (MQ=255)

     cGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCAt                                                                                          >  2:3036809/1‑100 (MQ=255)

     cGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCAt                                                                                          >  2:1594105/1‑100 (MQ=255)

     cGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCAt                                                                                          >  1:2399927/1‑100 (MQ=255)

      gATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATg                                                                                         >  1:1040776/1‑100 (MQ=255)

      gATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATg                                                                                         >  1:2394808/1‑100 (MQ=255)

       aTAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGc                                                                                        <  1:3082705/100‑1 (MQ=255)

       aTAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGc                                                                                        >  2:433012/1‑100 (MQ=255)

        tAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGcc                                                                                       <  1:4042289/100‑1 (MQ=255)

        tAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGcc                                                                                       <  2:283646/100‑1 (MQ=255)

           agaagGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGt                                                                                    >  1:3076232/1‑100 (MQ=255)

           agaagGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGt                                                                                    >  1:3677493/1‑100 (MQ=255)

             aagGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTgg                                                                                  <  2:1045931/100‑1 (MQ=255)

             aagGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTGCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTgg                                                                                  <  1:357508/100‑1 (MQ=255)

               ggCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCg                                                                                >  1:1975270/1‑100 (MQ=255)

                 cgcATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTa                                                                              >  1:206747/1‑100 (MQ=255)

                  gcATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCACTTCCAACGAAAGTCGTCGACACCATTGGGGCGGGCGCCAGTCACGCCGGTGGCGTAc                                                                             <  2:798490/100‑1 (MQ=255)

                    aTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACtt                                                                           >  1:2626835/1‑100 (MQ=255)

                     tGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTg                                                                          <  1:949370/100‑1 (MQ=255)

                        tATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCg                                                                       >  2:2535202/1‑100 (MQ=255)

                        tATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCg                                                                       >  1:1822772/1‑100 (MQ=255)

                         atgtCAGCAACGACACTGCTGGCGTCATTCCTGCATGTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATCCCGGTGGCGTACTTGCCgg                                                                      <  2:3660221/97‑1 (MQ=255)

                         aTTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCgg                                                                      >  1:495522/1‑100 (MQ=255)

                         aTTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCgg                                                                      <  2:1019150/100‑1 (MQ=255)

                         aTTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCgg                                                                      <  1:3512515/100‑1 (MQ=255)

                         aTTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCgg                                                                      >  1:1842959/1‑100 (MQ=255)

                          tttCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCggg                                                                     <  1:1100927/100‑1 (MQ=255)

                            tCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCt                                                                   <  2:3339288/100‑1 (MQ=255)

                             cAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTg                                                                  <  2:1272117/100‑1 (MQ=255)

                             cAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTg                                                                  >  2:935916/1‑100 (MQ=255)

                              aGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTgn                                                                 <  2:3935113/100‑2 (MQ=255)

                              aGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTgg                                                                 >  2:2272634/1‑100 (MQ=255)

                               gCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGc                                                                <  1:1833993/100‑1 (MQ=255)

                                cAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGcc                                                               <  1:3674092/100‑1 (MQ=255)

                                 aaCGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCct                                                              >  1:3032429/1‑100 (MQ=255)

                                     cgtcTTGTGGCGGCATTCAGGCAGTTCCAAGGGAAGTTGTCGACACCATTGGGGGGGGCGCCAGTCATGCCGGTGGCGTACTTTCCGGGCTGGCCTCTgg                                                          <  2:3624976/97‑1 (MQ=255)

                                     acgCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTgg                                                          <  1:1600299/100‑1 (MQ=255)

                                       gCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTc                                                        <  2:819784/100‑1 (MQ=255)

                                         ttCTGGCTGCATTCTGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTg                                                      <  2:872944/100‑1 (MQ=255)

                                         ttCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTg                                                      >  1:385481/1‑100 (MQ=255)

                                           cTGGCTGCATTCTGGCATTTCCAACGCAAGTTGTCGCCACCATTGGGGCCGGCGCCCGTCATGCCGGTGGCGTACTGGCCATGCTGCCCTCTGGTCTGcc                                                    <  2:1103064/100‑1 (MQ=255)

                                           cTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGcc                                                    >  2:3236698/1‑100 (MQ=255)

                                           cTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGcc                                                    <  1:3006921/100‑1 (MQ=255)

                                              gCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCCCt                                                 >  2:1708361/1‑100 (MQ=255)

                                               cTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTg                                                >  1:953907/1‑100 (MQ=255)

                                               cTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTg                                                >  2:3908885/1‑100 (MQ=255)

                                                  cATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCg                                             <  2:2355153/100‑1 (MQ=255)

                                                   aTTCCGGCATTTCCAACGCAAGTTGTCGACCCCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCgg                                            <  2:617249/100‑1 (MQ=255)

                                                   aTTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCgg                                            <  2:2617663/100‑1 (MQ=255)

                                                      ccGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATg                                         >  1:3353824/1‑100 (MQ=255)

                                                       cGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCTGGGCTGGCCTCTGGTCTGCCACTGGCGGATGc                                        <  1:499302/100‑1 (MQ=255)

                                                        ggCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGcc                                       <  2:813803/100‑1 (MQ=255)

                                                        ggCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTAGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGcc                                       >  1:3754326/1‑100 (MQ=255)

                                                          cATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGt                                     >  1:1657790/1‑100 (MQ=255)

                                                           aTTTCCAACGCAAGTTGTCGCCACCATTGGGGCGGGCGCCCGTCATGCCGGTGGCGTACTTGCCGTGCTGGCCTCTGGTCTGCCACTGGCGGCTGCCGtc                                    >  2:3593381/1‑99 (MQ=255)

                                                           aTTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTa                                    >  2:3593706/1‑100 (MQ=255)

                                                            tttCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTAt                                   >  2:200971/1‑100 (MQ=255)

                                                            tttCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTAt                                   <  2:3494229/100‑1 (MQ=255)

                                                             ttCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTAtt                                  <  2:3142990/100‑1 (MQ=255)

                                                              tCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTa                                 >  2:704792/1‑100 (MQ=255)

                                                               ccAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTAc                                >  2:3533047/1‑100 (MQ=255)

                                                                cAACGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACt                               <  1:20514/100‑1 (MQ=255)

                                                                 aaCGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTg                              <  1:476560/100‑1 (MQ=255)

                                                                 aaCGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTg                              <  1:1217132/100‑1 (MQ=255)

                                                                 aaCGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTg                              <  2:3471098/100‑1 (MQ=255)

                                                                  aCGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTgg                             <  1:2748977/100‑1 (MQ=255)

                                                                   cGCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTggg                            >  1:633655/1‑100 (MQ=255)

                                                                    gCAAGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGc                           <  2:426284/100‑1 (MQ=255)

                                                                    gCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGc                           <  1:1127041/100‑1 (MQ=255)

                                                                      aaGTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCaa                         >  2:576191/1‑100 (MQ=255)

                                                                        gTTGTCGACACCATTGGGTCTTGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGTCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATg                       <  2:3201268/100‑1 (MQ=255)

                                                                        gTTGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATg                       <  2:1920718/100‑1 (MQ=255)

                                                                         ttGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGc                      <  2:2065678/100‑1 (MQ=255)

                                                                         ttGTCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGc                      <  1:3610702/100‑1 (MQ=255)

                                                                           gttgACGCCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAg                    <  2:1423585/97‑1 (MQ=255)

                                                                            tCGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGt                   >  1:695890/1‑100 (MQ=255)

                                                                             cGACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTg                  >  2:2008464/1‑100 (MQ=255)

                                                                              gACACCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTgg                 <  1:3162891/100‑1 (MQ=255)

                                                                                cacCATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGcg               <  2:485989/100‑1 (MQ=255)

                                                                                  ccATTGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGcgtc             >  2:1432917/1‑100 (MQ=255)

                                                                                     ttGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTg          <  2:702114/100‑1 (MQ=255)

                                                                                      tGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCTGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCTTATTTCTGGGCAATGCAGTGGCGTCGTgg         <  1:3803424/100‑1 (MQ=255)

                                                                                      tGGGGCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTgg         <  1:1721381/100‑1 (MQ=255)

                                                                                      tGGGGCGGGCGCCAGTCATGCCGGTAGCGTACTTGCCGGGCTGGCCTGTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTgg         >  1:3647167/1‑100 (MQ=255)

                                                                                       ggggCGGGCGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTggg        <  2:1762219/100‑1 (MQ=255)

                                                                                        gggcgggcGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGt       <  2:1187061/100‑1 (MQ=255)

                                                                                        gggcgggcGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGt       >  2:3555560/1‑100 (MQ=255)

                                                                                        gggcgggcGCCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGt       <  1:1946998/100‑1 (MQ=255)

                                                                                        gggcgggcGCCAGTCATGCCGGTGGCGGACTTGCCGGGCTGGCGTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGt       <  1:1453016/100‑1 (MQ=255)

CGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCG  >  NC_000913/2181804‑2181996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`