Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,281,112 A→C I161L (ATC→CTC)  yejH → putative ATP‑dependent DNA or RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,281,1120AC96.4% 464.1 / 0.1 140I161L (ATC→CTC) yejHputative ATP‑dependent DNA or RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (66/69);  total (69/71)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

AGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAAC  >  NC_000913/2281017‑2281208
                                                                                               |                                                                                                
aGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTa                                                                                              >  1:1452146/1‑100 (MQ=255)
  tATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTAcc                                                                                            <  2:1662246/100‑1 (MQ=255)
  tATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTAcc                                                                                            <  2:624990/100‑1 (MQ=255)
    tCAGCAAATCCTCACTCACCTGACAAAAGTGAATCTCCAATTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAATGGCTGGCTGTACCat                                                                                          >  2:790762/1‑99 (MQ=255)
    tCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAg                                                                                          <  1:2612270/100‑1 (MQ=255)
    tCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAg                                                                                          >  2:3934477/1‑100 (MQ=255)
    tCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAg                                                                                          <  2:2335425/100‑1 (MQ=255)
     cAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGGTTGGGCAAAGGCTGGCTCTGCCTGt                                                                                         >  1:902727/1‑100 (MQ=255)
      aGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCTACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGtt                                                                                        >  1:3903503/1‑100 (MQ=255)
      aGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGtt                                                                                        >  1:3506966/1‑100 (MQ=255)
      aGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGtt                                                                                        <  1:2592267/100‑1 (MQ=255)
      aGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACCCCTTTTCGATTGGGCAAACGCTGGCTCTACCAGtt                                                                                        <  2:1774523/100‑1 (MQ=255)
       gCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGttt                                                                                       <  2:2931552/100‑1 (MQ=255)
        cAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTc                                                                                      <  1:3097716/100‑1 (MQ=255)
         aaaTCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCa                                                                                     <  1:952927/100‑1 (MQ=255)
           ctCCTCACTCACCTCACAAAAGTGGGTCCCCACTTACGCCTGCTGGGGCTGGATCCCACGCCCTTTCGACTGGGCAAAGCCTGCCTCTAACAGTGTCAtt                                                                                   <  2:1809973/99‑1 (MQ=255)
           aTCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCAtt                                                                                   <  1:344861/100‑1 (MQ=255)
              ctcactcaCCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCAACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACAAGTTTCATTATc                                                                                >  1:3456754/1‑100 (MQ=255)
                cactcacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCAc                                                                              >  2:45952/1‑100 (MQ=255)
                 actcacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACg                                                                             >  2:2744316/1‑100 (MQ=255)
                  ctcacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACgg                                                                            >  1:447718/1‑100 (MQ=255)
                   tcacCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGc                                                                           <  2:3668224/100‑1 (MQ=255)
                     acCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCAt                                                                         <  1:3685446/100‑1 (MQ=255)
                       cTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATgg                                                                       >  2:39207/1‑100 (MQ=255)
                        tGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGt                                                                      <  1:1789106/100‑1 (MQ=255)
                        tGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGt                                                                      <  1:1604384/100‑1 (MQ=255)
                         gACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCCCGGCATGGTa                                                                     >  2:218693/1‑100 (MQ=255)
                           cAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCCAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcg                                                                   >  1:2174195/1‑100 (MQ=255)
                           cAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcg                                                                   >  2:2361164/1‑100 (MQ=255)
                           cAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcg                                                                   <  2:2966266/100‑1 (MQ=255)
                           cAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcg                                                                   >  1:1761648/1‑100 (MQ=255)
                             aaaGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTAcgcg                                                                 <  1:208280/100‑1 (MQ=255)
                                gTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACgcggcg                                                              >  1:1045775/1‑100 (MQ=255)
                                  gAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGAt                                                            <  2:1025791/100‑1 (MQ=255)
                                   aaTCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTGCCAGTTTCATTATCACGGCATGGTACGCGGCGATg                                                           <  1:299767/100‑1 (MQ=255)
                                   aaTCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATg                                                           >  2:193997/1‑100 (MQ=255)
                                   aaTCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGAGTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATg                                                           <  2:3796385/100‑1 (MQ=255)
                                   aaTCCCCACCTACGCCTGCTTTGGCTGACTGCCACGCCTTTTGGGTTGGGCAAAGGCTGGCTCTACCAGTTTCATGATCTCGGGATGGTACGCGGCGATg                                                           <  2:1084257/100‑1 (MQ=255)
                                    aTCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATga                                                          <  1:334963/100‑1 (MQ=255)
                                    aTCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATga                                                          >  1:579588/1‑100 (MQ=255)
                                    aTCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATga                                                          >  1:1585956/1‑100 (MQ=255)
                                    aTCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATga                                                          <  2:2007456/100‑1 (MQ=255)
                                     tCCCCACTTACGCCTGCTGGGGCTGACTTCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATgag                                                         <  1:2798072/100‑1 (MQ=255)
                                     tCCCCACTTACGCCTGCTGGGGCTGACGGCCACGCCTTTTCTATTGGGCAAAGGCTGGCTCTACCAGGTTCATTATCACGGCATGGTACGCGGCGATgag                                                         <  2:2723283/100‑1 (MQ=255)
                                      ccccACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATCGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATgaga                                                        <  1:1406885/100‑1 (MQ=255)
                                      ccccACGTACGCCTCCTGTGGCTGACTGCCACGTCTTTTCGATTGGTCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGCTACGCGGCGATgaga                                                        <  2:3385788/100‑1 (MQ=255)
                                          tcTTACGCCTGCTGGGGCTGATTGCCACGCCTTTTCTATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGc                                                    <  2:3785052/99‑1 (MQ=255)
                                          aCTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTTTTTTTTTTTTAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGc                                                    >  2:2123865/1‑100 (MQ=255)
                                          aCTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGc                                                    <  1:1710456/100‑1 (MQ=255)
                                           cTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGcc                                                   <  1:2838484/100‑1 (MQ=255)
                                               cGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCttt                                               >  1:4029883/1‑100 (MQ=255)
                                               cGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCttt                                               >  1:2167546/1‑100 (MQ=255)
                                                 ccTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAACGGCTGGATATACCAGTTTCATTATCAGGGGATGGTATGGGGAGATGAGAAAGCCATTTTc                                             >  2:1591165/1‑100 (MQ=255)
                                                 ccTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTc                                             >  1:722547/1‑100 (MQ=255)
                                                 ccTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTc                                             >  1:1898446/1‑100 (MQ=255)
                                                 ccTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGAGGAGAAAGCCCTTTTc                                             >  1:736766/1‑100 (MQ=255)
                                                   tgctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCg                                           <  1:322591/100‑1 (MQ=255)
                                                   tgctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCg                                           <  2:2541144/100‑1 (MQ=255)
                                                   tgctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCg                                           >  2:1105394/1‑100 (MQ=255)
                                                     ctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTCCCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGtn                                         <  1:1176205/100‑2 (MQ=255)
                                                      tgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGa                                        >  1:1258846/1‑100 (MQ=255)
                                                       ggggCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGAc                                       <  1:490507/100‑1 (MQ=255)
                                                       ggggCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGAc                                       <  1:2894879/100‑1 (MQ=255)
                                                        gggCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACt                                      <  1:1014342/100‑1 (MQ=255)
                                                         ggCTGACTGCCACGCCTTTTTTTTTTTTTTTATGCTGGATATACCAGTTTCATTATCACTGTATGGTACGCGGCGATGAGAGAGCCCTTTTCCGTTACTg                                     >  2:1696202/1‑100 (MQ=255)
                                                           cTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCa                                   >  1:911422/1‑100 (MQ=255)
                                                            tGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCAt                                  >  1:2461174/1‑100 (MQ=255)
                                                               cTGCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTa                               >  1:2972289/1‑100 (MQ=255)
                                                                 gCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTGCCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATg                             <  2:2111676/100‑1 (MQ=255)
                                                                 gCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATg                             >  1:1691008/1‑100 (MQ=255)
                                                                 gCCACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATg                             <  1:1933032/100‑1 (MQ=255)
                                                                  ccACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGa                            <  2:3810069/100‑1 (MQ=255)
                                                                  ccACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGa                            >  1:353632/1‑100 (MQ=255)
                                                                   cACGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAg                           <  1:1039070/100‑1 (MQ=255)
                                                                    aCGCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGc                          <  2:1868087/100‑1 (MQ=255)
                                                                      gCCTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTg                        >  2:691272/1‑100 (MQ=255)
                                                                        cTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCCTTTATGAGCTGcc                      >  2:2969092/1‑100 (MQ=255)
                                                                        cTTTTCGATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGcc                      >  1:1361011/1‑100 (MQ=255)
                                                                              gATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCg                >  2:587324/1‑100 (MQ=255)
                                                                              gATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCg                >  1:1725094/1‑100 (MQ=255)
                                                                              gATTGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCg                >  1:3996718/1‑100 (MQ=255)
                                                                                 tGGGCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTta             <  2:2423310/100‑1 (MQ=255)
                                                                                  gggCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTtat            <  1:1671981/100‑1 (MQ=255)
                                                                                  gggCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTtat            <  1:3712169/100‑1 (MQ=255)
                                                                                   ggCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTtata           <  1:3100619/100‑1 (MQ=255)
                                                                                   ggCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTtata           >  1:3077793/1‑100 (MQ=255)
                                                                                   ggCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTtata           <  2:3295287/100‑1 (MQ=255)
                                                                                    gCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGGGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTatat          <  2:1647389/100‑1 (MQ=255)
                                                                                    gCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTatat          <  1:2064855/100‑1 (MQ=255)
                                                                                    gCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTatat          >  2:2229608/1‑100 (MQ=255)
                                                                                    gCAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACACGGCGATGAGAGAGCCCTTTTCCGTGGCTGCATTTATGAGCAGCCGCTGCGTTatat          <  1:3548268/100‑1 (MQ=255)
                                                                                     cAACGGCTGGCTCTACCAGGTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGATTGCATTTATGAGCTGCCGCTGCGTTATATg         >  2:3665299/1‑100 (MQ=255)
                                                                                     cAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATg         >  2:480809/1‑100 (MQ=255)
                                                                                     cAAAGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATg         <  2:2355141/100‑1 (MQ=255)
                                                                                       aaGGCTGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGAt       <  1:3013936/100‑1 (MQ=255)
                                                                                         ggctggctCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTa     >  1:2936048/1‑100 (MQ=255)
                                                                                         ggctggctCTACCAGTTTCATTAGCACAGCATGGTACGCTGAGATTAGAAAACCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTa     <  2:3177256/100‑1 (MQ=255)
                                                                                            tGGCTCTACCAGTTTCATTATCCAGGCATGGTACTCGGCGATGAGAAAGCCCTTATCCGTGACTGCATATATGAGCTGCCGCCGCCTTATATGACTAAac  <  2:334795/100‑1 (MQ=255)
                                                                                            tGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAac  <  1:4041185/100‑1 (MQ=255)
                                                                                            tGGCTCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAac  <  1:2969557/100‑1 (MQ=255)
                                                                                               |                                                                                                
AGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAAC  >  NC_000913/2281017‑2281208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑