Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,323,353 T→G intergenic (+102/‑94) atoC → / → atoD fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,323,3530TG98.6% 477.1 / ‑1.6 138intergenic (+102/‑94)atoC/atoDfused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (60/76);  total (61/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 139 total aligned reads displayed.

CAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATG  >  NC_000913/2323255‑2323449
                                                                                                  |                                                                                                
 aaaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTa                                                                                                >  1:636008/1‑100 (MQ=255)
  aaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAg                                                                                               >  2:788765/1‑100 (MQ=255)
     cTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTa                                                                                            >  1:128858/1‑100 (MQ=255)
     cTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTa                                                                                            >  1:129272/1‑100 (MQ=255)
     cTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTa                                                                                            <  1:754970/100‑1 (MQ=255)
     cTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTa                                                                                            <  1:2073178/100‑1 (MQ=255)
       tGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTaaa                                                                                          >  2:3318880/1‑100 (MQ=255)
       tGCTAGGCACAACTTTGTACAGTGCGCAATTTTCTGTATAGCTGTCCATTGTCCTTATATATCTCCATCCAATTTATCCCTTCATATTCAAGTTGCTaaa                                                                                          <  2:3760116/100‑1 (MQ=255)
       ggCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTaca                                                                                          >  2:3115023/2‑98 (MQ=21)
       ggCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTaaa                                                                                          >  1:741124/2‑100 (MQ=21)
        gCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAAt                                                                                         >  1:4085922/1‑100 (MQ=255)
        gCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAAt                                                                                         >  2:3981081/1‑100 (MQ=255)
         cTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATa                                                                                        <  1:4009979/100‑1 (MQ=255)
         cTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATa                                                                                        <  1:1579609/100‑1 (MQ=255)
         cTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATa                                                                                        >  1:2397065/1‑100 (MQ=255)
          tATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATaa                                                                                       >  1:1953331/1‑100 (MQ=255)
           aTGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAAc                                                                                      >  2:3242045/1‑100 (MQ=25)
           aTGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAAc                                                                                      >  1:3266999/1‑100 (MQ=25)
            tGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACt                                                                                     >  2:3770587/1‑100 (MQ=255)
            tGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATACTCAAGTCGTTACATAACt                                                                                     >  2:783956/1‑100 (MQ=21)
             tcAGAAATGGGCACATGTCGCAATGTTCTTCATATCCGCTCATTCTCCTTATAAATCCCCATCCAATATAGCCCTTCATATTCAAGTAGTTAAATAACTa                                                                                    >  2:4003020/2‑100 (MQ=255)
             gCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTAAAATAACTa                                                                                    <  2:2466789/100‑1 (MQ=255)
              cAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTaa                                                                                   >  2:2087173/1‑100 (MQ=255)
              cAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTaa                                                                                   >  2:2721425/1‑100 (MQ=255)
              cAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTaa                                                                                   >  2:2352101/1‑100 (MQ=25)
              cAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATGCAAGTAGTTACATAACTaa                                                                                   >  2:3569999/1‑100 (MQ=25)
               aGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTaaa                                                                                  <  1:1702377/100‑1 (MQ=255)
                gAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAAt                                                                                 <  1:689645/100‑1 (MQ=255)
                 aaaTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATc                                                                                <  1:1673243/100‑1 (MQ=255)
                 aaaTTTGCACAGTACGCATTTTTCTGCATAGTCGCTCATTCTTCTTATTTATCCCCAGCCACTTTATCCCTTCATATTCAAGTAGTTAAATAACaaaaca                                                                                <  2:1225449/100‑3 (MQ=255)
                  aaTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATcc                                                                               >  2:501115/1‑100 (MQ=255)
                   aTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCa                                                                              <  1:3070284/100‑1 (MQ=255)
                   aTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCa                                                                              <  1:129916/100‑1 (MQ=255)
                      tGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCaata                                                                           <  2:1499961/100‑1 (MQ=255)
                       gCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCaataa                                                                          <  1:863691/100‑1 (MQ=255)
                       gCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCaataa                                                                          >  2:1077771/1‑100 (MQ=255)
                       gCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCaataa                                                                          <  1:3417826/100‑1 (MQ=255)
                         acaGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAAtc                                                                        <  2:2250756/100‑1 (MQ=255)
                          caGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAACTTATCCCTTCTTATTCAAGTAGTTAAATAACTAAATCCAATAAtct                                                                       <  1:3941401/100‑1 (MQ=255)
                           aGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAAtctc                                                                      <  2:1076053/100‑1 (MQ=255)
                            gTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCa                                                                     >  1:359641/1‑100 (MQ=255)
                             ggcgcAATGTTCTGCAGAGCCGCTCTTTCTCCTTTTAGATCCCCATCCAATCTATCCCTTCATTTTCAAGTCGTTAAATAACTAAATCTAATAATCTCAt                                                                    <  2:1330183/99‑1 (MQ=255)
                              gtgcAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCAtt                                                                   >  2:2091198/3‑100 (MQ=255)
                               cgcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTc                                                                  <  1:3907093/100‑1 (MQ=255)
                               cgcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTc                                                                  <  1:781936/100‑1 (MQ=255)
                                gcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCt                                                                 <  1:1040572/100‑1 (MQ=255)
                                 cAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTg                                                                >  1:1762691/1‑100 (MQ=255)
                                 cAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTg                                                                >  1:981717/1‑100 (MQ=255)
                                  aaTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTgg                                                               <  1:2782135/100‑1 (MQ=255)
                                   aTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGc                                                              <  1:2785272/100‑1 (MQ=255)
                                   aTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGc                                                              >  2:2744767/1‑100 (MQ=255)
                                    ttttCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCa                                                             >  1:4040045/1‑100 (MQ=255)
                                        cTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTcc                                                         <  1:3682207/100‑1 (MQ=255)
                                         tGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTccc                                                        >  1:336032/1‑100 (MQ=255)
                                            aTAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCtt                                                     >  1:3765322/1‑100 (MQ=255)
                                              aGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGc                                                   <  1:3998711/100‑1 (MQ=255)
                                               gCCGCTCATTCTCCATATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCt                                                  >  2:3277841/1‑100 (MQ=255)
                                                   cTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTg                                              <  2:2061210/100‑1 (MQ=255)
                                                    tCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTTATTATGGCACTCCCCTTGCTATTGc                                             >  2:3783403/1‑100 (MQ=255)
                                                    tCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGc                                             <  2:2832072/100‑1 (MQ=255)
                                                       ttCTCCTTATAAATCCCCATCCAATGTAGCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTg                                          <  1:3603001/100‑1 (MQ=255)
                                                         ctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                                        <  2:28643/100‑1 (MQ=255)
                                                         ctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                                        <  2:2238544/100‑1 (MQ=255)
                                                         ctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                                        <  1:2383904/100‑1 (MQ=255)
                                                         ctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                                        <  1:4003478/100‑1 (MQ=255)
                                                         ctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                                        >  2:1034524/1‑100 (MQ=255)
                                                         ctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                                        <  1:413356/100‑1 (MQ=255)
                                                         ctcCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                                        <  2:1166212/100‑1 (MQ=255)
                                                           ccTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTg                                      >  1:3724009/1‑100 (MQ=255)
                                                           ccTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTg                                      <  2:3106828/100‑1 (MQ=255)
                                                           ccTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTg                                      <  1:3864511/100‑1 (MQ=255)
                                                             ttATAAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTa                                    <  2:1100048/100‑1 (MQ=255)
                                                              tataAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAc                                   <  1:2519637/100‑1 (MQ=255)
                                                              tataAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAc                                   <  1:1700114/100‑1 (MQ=255)
                                                              tataAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAc                                   <  2:1687128/100‑1 (MQ=255)
                                                               ataAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAcc                                  <  1:853407/100‑1 (MQ=255)
                                                                taAATCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAccc                                 <  1:2106350/100‑1 (MQ=255)
                                                                    tCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACaa                             >  1:654995/1‑100 (MQ=255)
                                                                    tCCCCATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACaa                             >  2:2613440/1‑100 (MQ=255)
                                                                      cccATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACg                           >  2:1750254/1‑100 (MQ=255)
                                                                       ccATCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACgg                          <  1:69182/100‑1 (MQ=255)
                                                                         aTCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGgtg                        <  2:2904217/100‑1 (MQ=255)
                                                                         aTCCAATTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACCCCCCTTGCTATTGCCTGACTGTACCCACAACGgtg                        >  2:2433459/1‑100 (MQ=255)
                                                                             aaTTTATCCCTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCAGTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATg                    >  2:1042599/1‑100 (MQ=255)
                                                                                    cccTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAgg             <  2:227179/100‑1 (MQ=255)
                                                                                     ccTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTATCCACAACGGTGTATGCAAGAggg            >  2:2365675/1‑100 (MQ=255)
                                                                                     ccTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAggg            >  1:2488571/1‑100 (MQ=255)
                                                                                     ccTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAggg            >  2:1443681/1‑100 (MQ=255)
                                                                                     ccTTCATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAggg            <  2:1656024/100‑1 (MQ=255)
                                                                                      cTTCATATTCAAGTAGGTAAATAACGAAATGCAATAATCTCAGTCTGGCACTCCGATTGCTATAGCCTGACTGTACCCACAACGGTGGATGCAAGAGGGa           >  2:655158/1‑100 (MQ=255)
                                                                                         cATATTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATaa        >  1:1387369/1‑100 (MQ=255)
                                                                                           tatTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATaaaa      >  1:3647215/1‑100 (MQ=255)
                                                                                           tatTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATaaaa      <  2:3556093/100‑1 (MQ=255)
                                                                                           tatTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATaaaa      >  1:1162167/1‑100 (MQ=255)
                                                                                           tatTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATaaaa      >  1:1130084/1‑100 (MQ=255)
                                                                                            atTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATaaaaa     <  1:261693/100‑1 (MQ=255)
                                                                                            atTCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATaaaaa     <  1:2119971/100‑1 (MQ=255)
                                                                                              tCAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAAt   <  2:625428/100‑1 (MQ=255)
                                                                                               cAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATg  >  2:196701/1‑100 (MQ=255)
                                                                                               cAAGTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATg  >  2:2114291/1‑100 (MQ=255)
                                                                                                  |                                                                                                
CAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATG  >  NC_000913/2323255‑2323449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: