BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,411,252	A→C	I34S (ATT→AGT)	yfbS ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,411,252	0	A	C	95.7%	536.1 / 8.1	162	I34S (ATT→AGT)	yfbS	putative transporter
Reads supporting (aligned to +/- strand): ref base A (4/3); new base C (74/81); total (78/84)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

ATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTT  >  NC_000913/2411156‑2411349

 taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTACTGCAAACGCGACACTaac                                                                                               >  2:3110301/1‑100 (MQ=255)

  aataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTaaca                                                                                              <  2:3115377/100‑1 (MQ=255)

  aataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTaaca                                                                                              >  1:2253452/1‑100 (MQ=255)

   ataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTaacaa                                                                                             >  2:2718255/1‑100 (MQ=255)

    taaGCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACGGTCAGCGTTCCGCTTAATGCAAACGCGACACTaacaaa                                                                                            <  2:1784072/100‑1 (MQ=255)

    naaaCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTaacaaa                                                                                            >  1:102077/2‑100 (MQ=255)

     aaaCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTaacaaac                                                                                           <  1:128575/100‑1 (MQ=255)

     aaaCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaacaaac                                                                                           <  1:2985384/100‑1 (MQ=255)

      aaCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACAGTCAGCGTTCCGCTTAATGCAAACGCGACACTaacaaaca                                                                                          <  1:1141646/100‑1 (MQ=255)

       aCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAacaaacaa                                                                                         <  1:868475/100‑1 (MQ=255)

        cAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAAcaaacaaa                                                                                        <  2:1707580/100‑1 (MQ=255)

        cAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAAcaaacaaa                                                                                        <  2:3691952/100‑1 (MQ=255)

         aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAg                                                                                       <  1:2073228/100‑1 (MQ=255)

              ggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGAcc                                                                                  <  2:2766532/100‑1 (MQ=255)

                   tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATc                                                                             <  1:452773/100‑1 (MQ=255)

                   tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATc                                                                             <  1:2773016/100‑1 (MQ=255)

                    cAGGACAACGTTCGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATcc                                                                            >  1:3515256/1‑100 (MQ=255)

                    cAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATcc                                                                            <  1:2109736/100‑1 (MQ=255)

                     aGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCa                                                                           >  1:3169422/1‑100 (MQ=255)

                      ggACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCAt                                                                          <  1:1846041/100‑1 (MQ=255)

                      ggACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCAt                                                                          >  2:1558690/1‑100 (MQ=255)

                        aCAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATAc                                                                        <  1:935025/100‑1 (MQ=255)

                           aCGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCa                                                                     <  2:1079309/100‑1 (MQ=255)

                           aCGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCa                                                                     >  1:3505231/1‑100 (MQ=255)

                            cGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCAc                                                                    >  1:1310912/1‑100 (MQ=255)

                                 ggATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGc                                                               <  2:2999455/100‑1 (MQ=255)

                                 ggATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGc                                                               >  2:3503952/1‑100 (MQ=255)

                                 ggATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGc                                                               <  1:3780142/100‑1 (MQ=255)

                                  gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGcc                                                              >  1:4028514/1‑100 (MQ=255)

                                  gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGcc                                                              <  1:2760107/100‑1 (MQ=255)

                                  gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGcc                                                              <  1:963423/100‑1 (MQ=255)

                                  gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGcc                                                              >  1:3672041/1‑100 (MQ=255)

                                    tCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCg                                                            <  2:464064/100‑1 (MQ=255)

                                     cAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGt                                                           >  1:2826037/1‑100 (MQ=255)

                                      aGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTc                                                          >  1:698795/1‑100 (MQ=255)

                                      aGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTc                                                          >  2:2695085/1‑100 (MQ=255)

                                      aGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTc                                                          >  1:1362473/1‑100 (MQ=255)

                                      aGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCCACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTc                                                          <  2:801545/100‑1 (MQ=255)

                                         aaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCa                                                       >  1:1493296/1‑100 (MQ=255)

                                           aGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCaaa                                                     <  1:366298/100‑1 (MQ=255)

                                            gCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAAc                                                    <  1:1787610/100‑1 (MQ=255)

                                             ccGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACa                                                   >  2:1890943/1‑100 (MQ=255)

                                             ccGGAAAATACTTCCGGGACAGTCAGCCTTCCGCTTCATGCAAACCCCACACTATCAAAGACAGCCACCGCATCCATACGCACTCTGCCCGTCGCAAACa                                                   <  2:298931/100‑1 (MQ=255)

                                              cGGAAAATACGTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACaa                                                  <  2:611133/100‑1 (MQ=255)

                                               ggAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAGAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAg                                                 <  2:2482116/100‑1 (MQ=255)

                                               ggAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAg                                                 <  1:850304/100‑1 (MQ=255)

                                                gAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGa                                                >  1:2425196/1‑100 (MQ=255)

                                                 aaaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGAc                                               <  1:1893332/100‑1 (MQ=255)

                                                    aTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGAt                                            <  2:1804113/100‑1 (MQ=255)

                                                    aTACTTCTGGGACTGTCAGCGTTCCCCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGAt                                            <  2:2235402/100‑1 (MQ=255)

                                                     tACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATg                                           >  1:1576352/1‑100 (MQ=255)

                                                     tACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATg                                           >  1:1578788/1‑100 (MQ=255)

                                                     tACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATg                                           <  2:2500085/100‑1 (MQ=255)

                                                      aCTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATgg                                          <  1:696744/100‑1 (MQ=255)

                                                      aCTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATgg                                          <  1:2733339/100‑1 (MQ=255)

                                                       cTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGc                                         <  2:19461/100‑1 (MQ=255)

                                                       cTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGc                                         >  1:215294/1‑100 (MQ=255)

                                                       cTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGc                                         <  2:3866994/100‑1 (MQ=255)

                                                           tGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACg                                     <  1:597378/100‑1 (MQ=255)

                                                           tGGGACTGTCAGCGTTCCGCTTAATGCAAACACGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACg                                     >  1:3273386/1‑100 (MQ=255)

                                                            gggTCTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACgg                                    >  2:3675530/1‑100 (MQ=255)

                                                            gggACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACAGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACgg                                    >  2:1995717/1‑100 (MQ=255)

                                                             ggACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGc                                   <  2:3666541/100‑1 (MQ=255)

                                                              gACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGcc                                  <  1:2886164/100‑1 (MQ=255)

                                                              gACTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGcc                                  <  2:2017673/100‑1 (MQ=255)

                                                                cTGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAg                                >  1:2880014/1‑100 (MQ=255)

                                                                 tGTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGt                               <  2:1890207/100‑1 (MQ=255)

                                                                  gTCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTa                              >  1:3709669/1‑100 (MQ=255)

                                                                   tCAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTaa                             >  1:1210474/1‑100 (MQ=255)

                                                                    cAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAAt                            >  2:1698202/1‑100 (MQ=255)

                                                                    cAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAAt                            >  1:2145102/1‑100 (MQ=255)

                                                                    cAGCGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAAt                            >  1:3678343/1‑100 (MQ=255)

                                                                       cGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGaa                         <  2:211795/100‑1 (MQ=255)

                                                                       cGTTCCGCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGaa                         >  2:3070676/1‑100 (MQ=255)

                                                                           ccGCTTAATGCAAACGCGACACTAACAAACAAGGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGaa                     <  1:1269597/100‑1 (MQ=255)

                                                                            cGCTTAATGCAAACGCGACACTAACAGACAAAGCGACCGCATCCATAGGCACTCAGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAGAGaag                    >  2:3732250/1‑99 (MQ=255)

                                                                             gCTTAATGGAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAcc                   >  2:3834058/1‑100 (MQ=255)

                                                                             gCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAcc                   <  2:2859530/100‑1 (MQ=255)

                                                                             gCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAcc                   <  1:1083718/100‑1 (MQ=255)

                                                                             gCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATCAAAGAAcc                   >  1:3894168/1‑100 (MQ=255)

                                                                               ttAATGCAAACGCGACACTAACAAACAAAGCAACCGCATCCATACGCACGCTACCCGTCGCAAACAAGACGATGGCACCGGCCAGTAATGAAAGAACCCa                 <  2:505121/100‑1 (MQ=255)

                                                                                tAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaa                >  2:1747686/1‑100 (MQ=255)

                                                                                tAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaa                >  1:3932036/1‑100 (MQ=255)

                                                                                 aaTGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa               >  2:2119562/1‑100 (MQ=255)

                                                                                 aaTGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa               <  2:2789309/100‑1 (MQ=255)

                                                                                 aaTGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa               <  1:241211/100‑1 (MQ=255)

                                                                                   tGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATc             <  1:213560/100‑1 (MQ=255)

                                                                                   tGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATc             <  2:1472055/100‑1 (MQ=255)

                                                                                   tGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATc             <  2:1467455/100‑1 (MQ=255)

                                                                                   tGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATc             >  2:1303171/1‑100 (MQ=255)

                                                                                   tGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATc             <  2:1214551/100‑1 (MQ=255)

                                                                                   tGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCAGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATc             >  2:3178395/1‑100 (MQ=255)

                                                                                   tGCAAACGCCACACTAACAAACATAGCGACCGCATCCATACGGACTCTGCCCGTCGCAAACAAGACGATCGCAACGGCCAGTAATGAAAGAACCCAAATc             <  1:3550537/100‑1 (MQ=255)

                                                                                    gCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGCCTATGGCAACGGCCAGTAATGAAAGAACCCAAATCa            <  2:2349046/100‑1 (MQ=255)

                                                                                    gCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa            <  1:265888/100‑1 (MQ=255)

                                                                                       aaCGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt         >  2:837918/1‑100 (MQ=255)

                                                                                         cgcgACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa       >  1:1481713/1‑100 (MQ=255)

                                                                                          gcgACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc      <  2:946888/100‑1 (MQ=255)

                                                                                          gcgACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc      <  1:761611/100‑1 (MQ=255)

                                                                                            gACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg    <  1:4002477/100‑1 (MQ=255)

ATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTT  >  NC_000913/2411156‑2411349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`