Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,483,809 T→G L19V (TTA→GTA)  evgA → response regulator in two‑component regulatory system with EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,483,8090TG90.7% 439.2 / 21.0 152L19V (TTA→GTA) evgAresponse regulator in two‑component regulatory system with EvgS
Reads supporting (aligned to +/- strand):  ref base T (6/8);  new base G (55/82);  total (61/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

CTACAGGGAGAAGGGAGATGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCAT  >  NC_000913/2483712‑2483903
                                                                                                 |                                                                                              
  acAGGGAGAAGGGAGATGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTAtt                                                                                            >  2:1365568/1‑100 (MQ=255)
   cAGGGAGAAGGGAGATGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTg                                                                                           <  1:1408753/100‑1 (MQ=255)
    agggagaagggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGa                                                                                          <  1:3965312/100‑1 (MQ=255)
     gggagaagggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGTAGCAATTCGTAATGTATTGAt                                                                                         <  1:1928062/100‑1 (MQ=255)
     gggagaagggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGAt                                                                                         >  2:38294/1‑100 (MQ=255)
     gggagaagggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGAt                                                                                         >  2:1535271/1‑100 (MQ=255)
     gggagaagggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGAt                                                                                         >  1:3557582/1‑100 (MQ=255)
      ggagaagggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATc                                                                                        >  1:3535758/1‑100 (MQ=255)
      ggagaagggagaTGCTTCATTGCAAAGGCAATAATCTATGAACGCAATAATGATGGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATc                                                                                        <  2:140111/100‑1 (MQ=255)
       gagaagggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGATCATCCTCTTGCTATCGCAGCAATTCGTAATGTAttattca                                                                                       <  2:3382390/100‑6 (MQ=255)
        agaagggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCaa                                                                                      <  1:1874254/100‑1 (MQ=255)
         gaagggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCaaa                                                                                     >  1:3703451/1‑100 (MQ=255)
          aagggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCaaaa                                                                                    >  1:917943/1‑100 (MQ=255)
            gggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAAc                                                                                  >  2:3038932/1‑100 (MQ=255)
            gggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAAc                                                                                  <  2:148338/100‑1 (MQ=255)
            gggagaTGCTTCATTGCAAAGGGAATAATCCATGAACGCAATAATGATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATTACAAAc                                                                                  <  2:3766118/100‑1 (MQ=255)
             ggagaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACg                                                                                 <  2:5745/100‑1 (MQ=255)
               agaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGat                                                                               <  1:3846029/100‑1 (MQ=255)
                gaTGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGata                                                                              <  1:631260/100‑1 (MQ=255)
                  tGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATAtt                                                                            <  1:2895775/100‑1 (MQ=255)
                  tGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAACTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATAtt                                                                            >  2:3576721/1‑100 (MQ=255)
                    cTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGa                                                                          >  1:3836300/1‑100 (MQ=255)
                    cTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGa                                                                          <  1:292600/100‑1 (MQ=255)
                     ttCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGaa                                                                         <  1:796161/100‑1 (MQ=255)
                      tCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGaaa                                                                        <  2:637298/100‑1 (MQ=255)
                      tCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGaaa                                                                        >  1:2767066/1‑100 (MQ=255)
                       cATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAAt                                                                       >  1:2817952/1‑100 (MQ=255)
                         ttGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCt                                                                     <  1:3015789/100‑1 (MQ=255)
                         ttGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCt                                                                     <  1:3015720/100‑1 (MQ=255)
                           gCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTa                                                                   >  2:3068353/1‑100 (MQ=255)
                           gCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTa                                                                   >  2:1274296/1‑100 (MQ=255)
                           gCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTa                                                                   <  2:2223869/100‑1 (MQ=255)
                           gCAAAGGGAATAATCTATGAACGCAAAAATTATTGATGACCATCATATTGCTATAGCAGCAATTCGTAATGTATTGATCAAAAACGAAATTGAAATCTTa                                                                   >  2:2619280/1‑100 (MQ=255)
                            cAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAg                                                                  <  1:1018027/100‑1 (MQ=255)
                            cAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAg                                                                  <  1:1771113/100‑1 (MQ=255)
                             aaaGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGc                                                                 >  2:3666417/1‑100 (MQ=255)
                              aaGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCa                                                                <  1:3106414/100‑1 (MQ=255)
                              aaGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCa                                                                <  1:1910362/100‑1 (MQ=255)
                              aaGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCa                                                                >  1:2349142/1‑100 (MQ=255)
                               aGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTTTTTTTTTTTTGTTTTAAAACGATATTGGTATCTTAGCag                                                               >  2:375526/1‑100 (MQ=255)
                               aGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATAGCAGCAATTCGTAGTGTATTGATCAAAAACGATATTGAAATCTTAGCag                                                               >  2:3946316/1‑100 (MQ=255)
                               aGGGAATAATCTATGAACGCAATAATTATTGATGACCAGCCTCTTGCCATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCag                                                               <  2:1600880/100‑1 (MQ=255)
                                gggAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCaga                                                              >  2:12270/1‑100 (MQ=255)
                                gggAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCaga                                                              >  1:460862/1‑100 (MQ=255)
                                 ggAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCagag                                                             <  2:411365/100‑1 (MQ=255)
                                  gAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGCGt                                                            <  1:2246432/100‑1 (MQ=255)
                                    ataatGTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCTCATCAATTCGTAATGTATTGATACAAAACGATATTGAAATCTTATCcgagctt                                                          >  2:3515116/1‑97 (MQ=255)
                                      aatCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGAc                                                        <  1:1344601/100‑1 (MQ=255)
                                       atCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACt                                                       <  2:2127712/100‑1 (MQ=255)
                                       atCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACt                                                       <  1:38960/100‑1 (MQ=255)
                                       atCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACt                                                       <  1:2912117/100‑1 (MQ=255)
                                        tCTATGAACGCAATAATTATTGATGGCCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTg                                                      <  2:2094974/100‑1 (MQ=255)
                                        tCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTg                                                      <  1:752137/100‑1 (MQ=255)
                                         cTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAAAAAAAAAAAAAAAAACGATATTGAAATCTTAGCAGAGTTGACTGa                                                     <  2:2851273/100‑1 (MQ=255)
                                             gAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGc                                                 >  2:2502798/1‑100 (MQ=255)
                                              aaCGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCg                                                >  1:1613917/1‑100 (MQ=255)
                                               aCGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCgg                                               >  2:1221113/1‑100 (MQ=255)
                                               aCGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCgg                                               <  1:3161130/100‑1 (MQ=255)
                                                 gCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGaa                                             >  2:1453382/1‑100 (MQ=255)
                                                 gCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGaa                                             <  1:153791/100‑1 (MQ=255)
                                                  cAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAg                                            >  2:3282735/1‑100 (MQ=255)
                                                  cAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAg                                            >  1:1295839/1‑100 (MQ=255)
                                                  cAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAg                                            <  2:3506254/100‑1 (MQ=255)
                                                   aataatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGt                                           >  1:235304/1‑100 (MQ=255)
                                                     taatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGc                                         >  1:3766032/1‑100 (MQ=255)
                                                     taatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGCGGTGACTGAAGGCGGAAGTGc                                         <  1:3483855/100‑1 (MQ=255)
                                                     taatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGc                                         <  2:2533704/100‑1 (MQ=255)
                                                      aatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGcc                                        <  1:2891828/100‑1 (MQ=255)
                                                      aatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGcc                                        <  1:2540802/100‑1 (MQ=255)
                                                      aatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGcc                                        <  1:1624850/100‑1 (MQ=255)
                                                      aatTATTGATGACCATCCTCTGGCTATCGTAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGcc                                        <  2:2979090/100‑1 (MQ=255)
                                                       attattGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCg                                       <  2:3597731/100‑1 (MQ=255)
                                                         tattGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGtt                                     <  2:836095/100‑1 (MQ=255)
                                                         tattGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGtt                                     >  1:2223755/1‑100 (MQ=255)
                                                          attGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTc                                    >  1:1428726/1‑100 (MQ=255)
                                                          attGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTc                                    <  1:3493068/100‑1 (MQ=255)
                                                            tgatgaCCATCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAg                                  >  1:85105/1‑100 (MQ=255)
                                                                 aCCATCCTCTTGCTATCGCAGCAATTCGTAATGTAGTGATCAAAAACGATGTTGAAAGGTTAGCAGAGTTAACTGAAGGCGGAAGTGCCGTTCTGCGGGt                             <  2:3065835/100‑1 (MQ=255)
                                                                  ccATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTg                            <  2:2968855/100‑1 (MQ=255)
                                                                    aTCCTCTTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGa                          <  2:3698192/100‑1 (MQ=255)
                                                                       ctctTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAac                       >  1:1143602/1‑100 (MQ=255)
                                                                        tctTGCTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAaca                      >  2:2779744/1‑100 (MQ=255)
                                                                             cTATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTaa                 >  2:1870886/1‑100 (MQ=255)
                                                                              tATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAg                <  2:1909291/100‑1 (MQ=255)
                                                                              tATCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAg                >  2:3980924/1‑100 (MQ=255)
                                                                                tCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGcc              <  1:1644128/100‑1 (MQ=255)
                                                                                tCGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGcc              >  1:2164952/1‑100 (MQ=255)
                                                                                 cGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCt             >  1:1685034/1‑100 (MQ=255)
                                                                                 cGCAGCAATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCt             >  1:3683110/1‑100 (MQ=255)
                                                                                  gcagcaATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTg            >  1:145254/1‑100 (MQ=255)
                                                                                     gcaATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGata         >  1:606301/1‑100 (MQ=255)
                                                                                     gcaATTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGata         >  1:2424215/1‑100 (MQ=255)
                                                                                        aTTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCg      <  1:2588930/100‑1 (MQ=255)
                                                                                        aTTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCg      <  1:1949251/100‑1 (MQ=255)
                                                                                        aTTCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCg      <  2:2206072/100‑1 (MQ=255)
                                                                                        aTTCGTAATGTATTGAGCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGGGGAAACACTTAAGCCTGATATCg      >  1:1792569/1‑100 (MQ=255)
                                                                                          tCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGtc    <  1:2282298/100‑1 (MQ=255)
                                                                                          tCGTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGtc    <  2:126264/100‑1 (MQ=255)
                                                                                           cGTAATGTATTGATCAAAAACGATAGTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGtca   <  2:3372540/100‑1 (MQ=255)
                                                                                            gTAATGTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGtcat  <  1:3765268/100‑1 (MQ=255)
                                                                                                 |                                                                                              
CTACAGGGAGAAGGGAGATGCTTCATTGCAAAGGGAATAATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCAT  >  NC_000913/2483712‑2483903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: