BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,488,822	A→C	L116R (CTT→CGT)	yfdE ←	acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,488,822	0	A	C	100.0%	392.5 / NA	110	L116R (CTT→CGT)	yfdE	acetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (56/54); total (56/54)

Only 100 of 111 total aligned reads displayed.

TGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTT  >  NC_000913/2488726‑2488917

tGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAcgcg                                                                                              >  1:413744/1‑100 (MQ=255)

   atgatgGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGttt                                                                                           >  2:1716569/1‑100 (MQ=255)

       tgGTATCGTAGGCAGGAGCATGTTTTAGCGGACCGGTATGTCCGAAAACTGACGATGAAGGATTTATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCa                                                                                       <  2:2826833/100‑1 (MQ=255)

       tgGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCa                                                                                       <  1:178200/100‑1 (MQ=255)

        ggTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCAt                                                                                      <  1:1455893/100‑1 (MQ=255)

          tATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGa                                                                                    <  1:3674486/100‑1 (MQ=255)

           aTCGTAGGCAGGAGCATCTTTTAGCGGACCGGTAAGTCCGAAACCTGACGATGGAGCATATAGGAGGCGCGGGTCGATTTCTTGACGCGTTTCCCATGaa                                                                                   >  1:3795262/1‑100 (MQ=255)

              gTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAAc                                                                                >  1:3888361/1‑100 (MQ=255)

               tAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAAcc                                                                               <  1:2487380/100‑1 (MQ=255)

                 ggCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAAcccc                                                                             >  2:3819410/1‑100 (MQ=255)

                   cAGGAGCATCTTTTAGCGGACCGGTATGTGAGAAACATGACGATGAAGCATATATGAGGCGCTGGTCAATTTGTTGACGCGTTTCCCATGAAGAccgaat                                                                           >  2:636379/1‑96 (MQ=255)

                   cAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAg                                                                           <  1:2426500/100‑1 (MQ=255)

                   cAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAg                                                                           <  1:999741/100‑1 (MQ=255)

                   cAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAg                                                                           >  2:117307/1‑100 (MQ=255)

                     ggAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGtt                                                                         <  1:810910/100‑1 (MQ=255)

                     ggAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGtt                                                                         <  1:2000919/100‑1 (MQ=255)

                     ggAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGtt                                                                         >  1:1262534/1‑100 (MQ=255)

                       aGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGtttt                                                                       <  1:2209794/100‑1 (MQ=255)

                       aGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGtttt                                                                       <  2:35253/100‑1 (MQ=255)

                        gCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGttttt                                                                      >  1:3858144/1‑100 (MQ=255)

                         cATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTc                                                                     <  2:528719/100‑1 (MQ=255)

                          aTCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTcc                                                                    <  1:123220/100‑1 (MQ=255)

                            cTTTTCGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCAt                                                                  >  2:3513508/1‑100 (MQ=255)

                            cTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCAt                                                                  >  1:2112997/1‑100 (MQ=255)

                            cTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCAt                                                                  <  2:2730982/100‑1 (MQ=255)

                            cTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCAt                                                                  >  1:2309921/1‑100 (MQ=255)

                              tttAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTg                                                                >  2:816342/1‑100 (MQ=255)

                              tttAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTg                                                                <  2:1048850/100‑1 (MQ=255)

                                   cGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCt                                                           >  2:2646531/1‑100 (MQ=255)

                                    ggACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTg                                                          >  1:727173/1‑100 (MQ=255)

                                    ggACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTg                                                          <  1:205895/100‑1 (MQ=255)

                                    ggACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTg                                                          >  1:3019254/1‑100 (MQ=255)

                                     gACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTgg                                                         <  1:2833469/100‑1 (MQ=255)

                                     gACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTgg                                                         <  2:136581/100‑1 (MQ=255)

                                      aCCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTggg                                                        <  1:554397/100‑1 (MQ=255)

                                      aCCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTggg                                                        <  1:1232968/100‑1 (MQ=255)

                                       ccGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGc                                                       >  2:347729/1‑100 (MQ=255)

                                         ggTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGa                                                     >  2:1764594/1‑100 (MQ=255)

                                         ggTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGa                                                     >  1:1829459/1‑100 (MQ=255)

                                         ggTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGa                                                     >  1:280581/1‑100 (MQ=255)

                                         ggTATGTCAGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCTAGTTTTTGCATTGTACCTGGGCGa                                                     >  2:1147402/1‑100 (MQ=255)

                                           tATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGaaa                                                   >  2:137310/1‑100 (MQ=255)

                                           tATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGaaa                                                   <  1:2468703/100‑1 (MQ=255)

                                             tGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAAt                                                 >  2:3048289/1‑100 (MQ=255)

                                             tGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAAt                                                 >  1:2619747/1‑100 (MQ=255)

                                             tGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAAt                                                 <  1:2408485/100‑1 (MQ=255)

                                              gTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTGTCCCATGAAAACCCCAGTTTTTCCATCGTACCTGGGCGAAAAtt                                                >  1:3742548/1‑100 (MQ=255)

                                               tCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATtc                                               >  1:3617755/1‑100 (MQ=255)

                                               tCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATtc                                               <  1:566737/100‑1 (MQ=255)

                                                ccGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATtct                                              >  1:200450/1‑100 (MQ=255)

                                                ccGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATtct                                              >  2:875287/1‑100 (MQ=255)

                                                 cGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATtctc                                             <  1:2310454/100‑1 (MQ=255)

                                                  gAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCa                                            >  1:3393822/1‑100 (MQ=255)

                                                    aaCCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGGTTTTCCATTGTACCTGGGCGAAAATTCTCAGc                                          >  2:513150/1‑100 (MQ=255)

                                                     aCCTGACGATGAAGCATTTATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCt                                         <  2:2467897/100‑1 (MQ=255)

                                                       cTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTaa                                       >  1:3955594/1‑100 (MQ=255)

                                                       cTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTaa                                       >  1:3955578/1‑100 (MQ=255)

                                                        tGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTaan                                      <  1:3251876/100‑2 (MQ=255)

                                                        tGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAAt                                      <  2:2337620/100‑1 (MQ=255)

                                                        tGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAAt                                      <  1:3392704/100‑1 (MQ=255)

                                                        tGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAAt                                      >  2:1003266/1‑100 (MQ=255)

                                                           cGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACa                                   >  2:3977202/1‑100 (MQ=255)

                                                           cGATGAAGCATATATGAGGCACGGGTTGATGTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACATGGGCGAAAATTCTCAGCTAAAACa                                   >  2:592330/1‑100 (MQ=255)

                                                             aTGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATc                                 <  1:354052/100‑1 (MQ=255)

                                                             aTGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATc                                 <  1:3229020/100‑1 (MQ=255)

                                                              tGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCa                                >  1:2293147/1‑100 (MQ=255)

                                                                aaGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGc                              <  2:3192574/100‑1 (MQ=255)

                                                                 aGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCt                             >  1:2099457/1‑100 (MQ=255)

                                                                     tatatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGtt                         >  2:405527/1‑100 (MQ=255)

                                                                     tatatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGtt                         <  2:570049/100‑1 (MQ=255)

                                                                     tatatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGtt                         <  1:241527/100‑1 (MQ=255)

                                                                     tatatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGtt                         >  1:739238/1‑100 (MQ=255)

                                                                       tatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACTCCAGTTTTTCCATTGTATCTGTGCGAAAATTCTCAGCTAATACATCAGATTGTTTg                       >  2:2263994/1‑100 (MQ=255)

                                                                       tatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTTCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTg                       >  2:2389556/1‑100 (MQ=255)

                                                                       tatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTg                       >  2:2477148/1‑100 (MQ=255)

                                                                       tatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTg                       <  1:204164/100‑1 (MQ=255)

                                                                        atGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGa                      <  2:2418215/100‑1 (MQ=255)

                                                                         tGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAg                     <  1:2163245/100‑1 (MQ=255)

                                                                         tGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAg                     <  1:894121/100‑1 (MQ=255)

                                                                         tGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAg                     <  1:147833/100‑1 (MQ=255)

                                                                          gAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTACTACATCAGCTTGTTTGAGc                    <  2:1698567/100‑1 (MQ=255)

                                                                          gAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGc                    <  2:2188275/100‑1 (MQ=255)

                                                                          gAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGc                    <  2:2670959/100‑1 (MQ=255)

                                                                          gAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGc                    <  2:723893/100‑1 (MQ=255)

                                                                            ggCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCat                  >  1:2907582/1‑100 (MQ=255)

                                                                            ggCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCat                  <  1:1399231/100‑1 (MQ=255)

                                                                                cgGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATAttt              <  1:3630678/100‑1 (MQ=255)

                                                                                 gggTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTta             <  1:2880078/100‑1 (MQ=255)

                                                                                  ggTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTTTTTTTTTTTTTTGCGAAAATTCTCAGCTAATACATCTGCTTGTTTGACCATATTtat            >  2:3970699/1‑100 (MQ=255)

                                                                                   gTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTtata           <  1:1165879/100‑1 (MQ=255)

                                                                                       aTTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAata       <  2:3537763/100‑1 (MQ=255)

                                                                                       aTTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAata       >  2:1295041/1‑100 (MQ=255)

                                                                                       aTTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAata       >  2:1646171/1‑100 (MQ=255)

                                                                                       aTTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAata       >  1:1490304/1‑100 (MQ=255)

                                                                                        tttCTTGTCGCGTTTCCCATGAAAACCCTAGTTTTTCCATTGTACCTGGGCGAAAATTCTTAGCTAATACATCAGCTTGTTTGAGCAGATTTATAAatat      <  2:3942486/100‑1 (MQ=255)

                                                                                          tCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATAc    >  1:3337593/1‑100 (MQ=255)

                                                                                          tCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATAc    >  1:3061636/1‑100 (MQ=255)

                                                                                           cTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACt   >  1:2700324/1‑100 (MQ=255)

                                                                                            ttGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACtt  >  1:3696994/1‑100 (MQ=255)

                                                                                            ttGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACtt  <  2:3353522/100‑1 (MQ=255)

TGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTT  >  NC_000913/2488726‑2488917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`