BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,717,422	A→C	V223V (GTA→GTC)	ung →	uracil‑DNA‑glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,422	0	A	C	97.7%	581.0 / 2.6	173	V223V (GTA→GTC)	ung	uracil‑DNA‑glycosylase
Reads supporting (aligned to +/- strand): ref base A (3/1); new base C (79/90); total (82/91)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01

Only 100 of 173 total aligned reads displayed.

TTCGGCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCA  >  NC_000913/2717326‑2717518

ttCGGCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTa                                                                                               <  1:1007165/100‑1 (MQ=255)

  cGGCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTAcc                                                                                             <  2:1300557/100‑1 (MQ=255)

   ggCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCg                                                                                            <  2:2725341/100‑1 (MQ=255)

   ggCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCg                                                                                            <  2:3143305/100‑1 (MQ=255)

   ggCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCg                                                                                            >  1:3315789/1‑100 (MQ=255)

    gcgcATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCgg                                                                                           <  2:2260391/100‑1 (MQ=255)

     cgcATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGc                                                                                          <  2:2093708/100‑1 (MQ=255)

      gcATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCa                                                                                         <  1:2531295/100‑1 (MQ=255)

        aTCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCaga                                                                                       >  2:1066684/1‑100 (MQ=255)

        aTCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCaga                                                                                       <  1:1649767/100‑1 (MQ=255)

           gTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCagagag                                                                                    >  2:3488317/1‑100 (MQ=255)

           gTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCagagag                                                                                    >  1:3380866/1‑100 (MQ=255)

            tGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagt                                                                                   >  2:3619326/1‑100 (MQ=255)

             ggATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtg                                                                                  <  1:1713344/100‑1 (MQ=255)

             ggATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtg                                                                                  <  1:3759351/100‑1 (MQ=255)

             ggATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtg                                                                                  >  1:2175895/1‑100 (MQ=255)

             ggATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtg                                                                                  <  2:1707260/100‑1 (MQ=255)

             ggATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtg                                                                                  <  2:2986209/100‑1 (MQ=255)

              gATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtga                                                                                 >  2:1529407/1‑100 (MQ=255)

               aTTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtgag                                                                                <  2:423012/100‑1 (MQ=255)

                ttCTTTGGCTGCAACCATTTTGTGCTGTCAAATCAGTGGCATGATCAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtgagt                                                                               <  2:3816597/100‑1 (MQ=255)

                ttCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAgagtgagt                                                                               >  1:979395/1‑100 (MQ=255)

                 tCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGagtaagta                                                                              <  1:1603883/100‑1 (MQ=255)

                  cTTTGGCTGCAACCATTTTGTACTGCCAAATCAGTGCCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTaa                                                                             <  2:3791499/100‑1 (MQ=255)

                       gCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTg                                                                        <  1:3119501/100‑1 (MQ=255)

                         tGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCg                                                                      <  1:3032828/100‑1 (MQ=255)

                         tGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCg                                                                      >  1:2929295/1‑100 (MQ=255)

                         tGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCg                                                                      <  2:1518537/100‑1 (MQ=255)

                         tGCAACCATGTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCg                                                                      <  2:1922682/100‑1 (MQ=255)

                         tGCAACCATATGGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAAACGCCGATTGACTGGATGACAGTCTTACCGGCAGAGAGTGAGTAAATTTGCg                                                                      <  2:47298/100‑1 (MQ=255)

                          gCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCgg                                                                     <  2:243389/100‑1 (MQ=255)

                          gCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCgg                                                                     >  1:2215313/1‑100 (MQ=255)

                           cAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCggg                                                                    >  1:1572668/1‑100 (MQ=255)

                            aaCCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCgggg                                                                   >  2:3888222/1‑100 (MQ=255)

                                aTTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAAt                                                               >  1:3029676/1‑100 (MQ=255)

                                aTTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAAt                                                               <  1:517552/100‑1 (MQ=255)

                                 ttttGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATg                                                              <  1:782614/100‑1 (MQ=255)

                                       gCTTGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTTAGTAAATTTGCGTGGAAATGCCGGAt                                                        <  2:193531/100‑1 (MQ=255)

                                       gCTGGCCGATGAGTGGCTGGAACCACGTGGCGAGACGACGATTGACTGGCTGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGCGAAATGCCGGAt                                                        <  2:2835631/100‑1 (MQ=255)

                                       gCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGAt                                                        <  1:1178596/100‑1 (MQ=255)

                                          ggCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGc                                                     >  2:1811822/1‑100 (MQ=255)

                                          ggCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGc                                                     <  2:3032872/100‑1 (MQ=255)

                                            cAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCag                                                   <  2:1507686/100‑1 (MQ=255)

                                            cAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCag                                                   <  2:795855/100‑1 (MQ=255)

                                             aaaTCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCaga                                                  >  1:90154/1‑100 (MQ=255)

                                              naTCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCagag                                                 >  1:3242792/2‑100 (MQ=255)

                                              aaTCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCagag                                                 <  2:796435/100‑1 (MQ=255)

                                                 cAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTg                                              <  2:958935/100‑1 (MQ=255)

                                                 cAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTg                                              <  1:4017467/100‑1 (MQ=255)

                                                 cAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTg                                              <  1:2840737/100‑1 (MQ=255)

                                                  aGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGc                                             <  2:719497/100‑1 (MQ=255)

                                                  aGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGc                                             >  1:1231206/1‑100 (MQ=255)

                                                    caccTGGAACAACGTGGCCAGAAGCGAATTGACTGGATGCAAGTCTTACCGGCAGAGCGTGAGTAAATTTGCGGGGAAATGCAGGATGGCCGAGTTGcaa                                           <  2:1181348/97‑3 (MQ=255)

                                                     ggCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCTGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCAc                                          <  1:3606744/100‑1 (MQ=255)

                                                     ggCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCAc                                          >  1:1381440/1‑100 (MQ=255)

                                                     ggCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCAc                                          >  1:1380978/1‑100 (MQ=255)

                                                     ggCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCAc                                          >  1:27376/1‑100 (MQ=255)

                                                      gCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCAcc                                         >  2:2384900/1‑100 (MQ=255)

                                                       cTGGAACAACGTGGCGAGTCTCCGATTGCCTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAACTTTCCGGGGATAAGACTCCTGGCAGATTTGCCAccc                                        <  2:1762636/100‑1 (MQ=255)

                                                       cTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCAccc                                        >  1:2270781/1‑100 (MQ=255)

                                                            acaacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTg                                   >  2:3118833/1‑100 (MQ=255)

                                                            acaacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTg                                   <  1:3445112/100‑1 (MQ=255)

                                                            acaacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGGGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTg                                   >  1:1102288/1‑100 (MQ=255)

                                                             caacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGa                                  >  2:2158155/1‑100 (MQ=255)

                                                             caacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCAGCAGAGAGTGAGCAAATTTGCGGGGAAATGCCGGGTGGCAGAGTCGGCACCCGGCTGa                                  <  2:2949976/100‑1 (MQ=255)

                                                              aacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGAt                                 <  1:686405/100‑1 (MQ=255)

                                                              aacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGAt                                 <  2:484328/100‑1 (MQ=255)

                                                              aacGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGAt                                 <  1:479489/100‑1 (MQ=255)

                                                               acGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGAtt                                >  2:771370/1‑100 (MQ=255)

                                                                cGTGGCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGAttt                               >  2:1960117/1‑100 (MQ=255)

                                                                   ggCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATc                            >  1:245921/1‑100 (MQ=255)

                                                                    gCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCa                           <  1:1254143/100‑1 (MQ=255)

                                                                    gCGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCa                           <  1:2702794/100‑1 (MQ=255)

                                                                     cGAGACGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAg                          <  1:1020260/100‑1 (MQ=255)

                                                                       agaCGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGc                        <  1:900713/100‑1 (MQ=255)

                                                                       agaCGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGc                        <  2:26948/100‑1 (MQ=255)

                                                                       agaCGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGc                        <  1:2554915/100‑1 (MQ=255)

                                                                        gaCGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCt                       >  1:759596/1‑100 (MQ=255)

                                                                          cGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCttt                     >  1:3531660/1‑100 (MQ=255)

                                                                          cGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCttt                     >  1:61992/1‑100 (MQ=255)

                                                                          cGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCttt                     >  2:1172032/1‑100 (MQ=255)

                                                                          cGCCGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCttt                     >  1:384661/1‑100 (MQ=255)

                                                                            ccGATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTAt                   >  1:195230/1‑100 (MQ=255)

                                                                              gATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTc                 <  1:1400151/100‑1 (MQ=255)

                                                                              gATTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTc                 >  1:2218182/1‑100 (MQ=255)

                                                                               aTTGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCt                >  1:1186170/1‑100 (MQ=255)

                                                                                 tGACTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGa              <  2:396370/100‑1 (MQ=255)

                                                                                   aCTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCAGGCGGCTGATTTATCAGGCTTTATTCTGACg            <  2:895978/100‑1 (MQ=255)

                                                                                   aCTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACg            <  2:327310/100‑1 (MQ=255)

                                                                                   aCTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACg            <  2:2181325/100‑1 (MQ=255)

                                                                                   aCTGGATGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACg            >  1:2092322/1‑100 (MQ=255)

                                                                                    cTGGATGCCAGTCTTACCGGCGGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGc           >  2:1734005/1‑100 (MQ=255)

                                                                                        aTGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGccacc       <  1:3542716/100‑1 (MQ=255)

                                                                                         tGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGccacca      >  2:1536975/1‑100 (MQ=255)

                                                                                         tGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGccacca      >  1:466751/1‑100 (MQ=255)

                                                                                         tGCCAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGccacca      >  1:1934017/1‑100 (MQ=255)

                                                                                           ccAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCAtt    >  1:3793121/1‑100 (MQ=255)

                                                                                           ccAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCAtt    >  1:3509155/1‑100 (MQ=255)

                                                                                           caaGTCTTACCCGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGTCACCAtt    >  2:3707600/3‑100 (MQ=255)

                                                                                            cAGTCTTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTc   >  1:259771/1‑100 (MQ=255)

TTCGGCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCA  >  NC_000913/2717326‑2717518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`