Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,854,496 A→C Q53H (CAA→CAC)  fhlA → formate hydrogenlyase transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,854,4960AC90.2% 405.8 / 24.3 143Q53H (CAA→CAC) fhlAformate hydrogenlyase transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (7/7);  new base C (55/74);  total (62/81)
Fisher's exact test for biased strand distribution p-value = 7.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

ATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACG  >  NC_000913/2854400‑2854591
                                                                                                |                                                                                               
gttGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAcgcg                                                                                              <  2:3155158/99‑1 (MQ=255)
aTTGCAGCAGCCCGATCTGGCCTCGCTGTGTGTGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAcgcg                                                                                              >  2:3600586/1‑100 (MQ=255)
aTTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAcgcg                                                                                              >  2:2577099/1‑100 (MQ=255)
aTTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCGGCGCTCGGCGACCACGGGGCTATTGTGTTGTGGCAcgcg                                                                                              >  2:764580/1‑100 (MQ=255)
 ttGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACgcgc                                                                                             <  1:3796428/100‑1 (MQ=255)
 ttGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACgcgc                                                                                             <  2:3603464/100‑1 (MQ=255)
  tGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCa                                                                                            <  2:3609756/100‑1 (MQ=255)
   gcagcaGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAg                                                                                           <  1:269077/100‑1 (MQ=255)
   gcagcaGCCCGATCTGGCCTCGCTGTGTGAGGCTCTCTCGAAACTGGTAGAGCGCTCTGCGCTCGCCGATAACGCGGCTATTGTGTTGTGGCACGCGCAg                                                                                           >  2:3758131/1‑100 (MQ=255)
    cagcagCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGa                                                                                          >  1:2330800/1‑100 (MQ=255)
     agcagcCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGAc                                                                                         <  1:2782201/100‑1 (MQ=255)
      gcagcCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACt                                                                                        <  2:3858219/100‑1 (MQ=255)
       cagcCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTc                                                                                       <  2:645951/100‑1 (MQ=255)
        agcCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCa                                                                                      <  2:1808435/100‑1 (MQ=255)
        agcCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCGCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCa                                                                                      <  1:3473034/100‑1 (MQ=255)
         gcCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCaa                                                                                     >  1:1949154/1‑100 (MQ=255)
         gcCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCaa                                                                                     >  2:767096/1‑100 (MQ=255)
         gcCCGAGCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGTTAAAGCGTACTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAAACTCaa                                                                                     >  2:3762453/1‑100 (MQ=255)
            cGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGt                                                                                  <  2:1449201/100‑1 (MQ=255)
            cGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGt                                                                                  <  1:3315057/100‑1 (MQ=255)
             gATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGtt                                                                                 <  1:2479645/100‑2 (MQ=255)
             gATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTg                                                                                 <  1:804307/100‑1 (MQ=255)
             gATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTg                                                                                 <  1:1520069/100‑1 (MQ=255)
               tCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCg                                                                               <  1:2451270/100‑1 (MQ=255)
               tCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCg                                                                               <  2:46776/100‑1 (MQ=255)
                cTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGt                                                                              >  1:2999864/1‑100 (MQ=255)
                  ggCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCt                                                                            >  1:745/1‑100 (MQ=255)
                   gCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCtt                                                                           <  1:1601042/100‑1 (MQ=255)
                   gCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCCTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCtt                                                                           >  2:1916772/1‑100 (MQ=255)
                    ccTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCtta                                                                          >  1:1898300/1‑100 (MQ=255)
                    ccTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGCCTATGGTGTTGTGGCACGCGCAGACGCAACGCGCGTCtta                                                                          >  2:3378002/1‑100 (MQ=255)
                     cTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTTTGGCACGCGCAGACTCACCGTGCGTCttat                                                                         >  2:3400230/1‑100 (MQ=255)
                        gCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTAc                                                                      <  2:2776551/100‑1 (MQ=255)
                        gCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGACGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACCCAACGTGCGTCTTATTAc                                                                      >  2:3786109/1‑100 (MQ=255)
                         cTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTAcg                                                                     <  2:1291311/100‑1 (MQ=255)
                              tgAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTAcgcgtcg                                                                >  2:3922657/1‑100 (MQ=255)
                               gAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGATATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTAcgcgtcgc                                                               >  2:3841577/1‑100 (MQ=255)
                                aGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTAcgcgtcgcg                                                              <  1:3804583/100‑1 (MQ=255)
                                aGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTAcgcgtcgcg                                                              <  2:198622/100‑1 (MQ=255)
                                  gCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTg                                                            <  1:3923390/100‑1 (MQ=255)
                                  gCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTg                                                            <  1:2268425/100‑1 (MQ=255)
                                      tttCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGaaaa                                                        <  1:3681408/100‑1 (MQ=255)
                                        tCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAg                                                      <  1:2068151/100‑1 (MQ=255)
                                        tCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAg                                                      <  1:2786635/100‑1 (MQ=255)
                                          ncAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCGACGTGCGTCTTATTACGCGTCGCGTGTAAAAGac                                                    >  1:3263098/2‑100 (MQ=255)
                                          gCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGac                                                    >  2:2155658/1‑100 (MQ=255)
                                          gCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGac                                                    <  1:3838922/100‑1 (MQ=255)
                                           cAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGaca                                                   >  2:357054/1‑100 (MQ=255)
                                              cTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACAccc                                                <  2:2549278/100‑1 (MQ=255)
                                               tGGTAAAGCGTTGTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACAcccc                                               <  2:2828105/100‑1 (MQ=255)
                                               tGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACAcccc                                               >  1:2688480/1‑100 (MQ=255)
                                                ggTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACAccccc                                              >  1:1601847/1‑100 (MQ=255)
                                                ggTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACAccccc                                              <  2:2445572/100‑1 (MQ=255)
                                                ggTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACAccccc                                              >  2:3641369/1‑100 (MQ=255)
                                                ggTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGAAAGAGCAGACTAAACGTGCGTATTATTAAGAGAAGAGTGAAAAAGACAccccc                                              <  2:158262/100‑1 (MQ=255)
                                                 gTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCa                                             >  1:2435186/1‑100 (MQ=255)
                                                   aaaGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCAtt                                           >  2:135931/1‑100 (MQ=255)
                                                     aGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGGTGTGGCACGCGCAGACTCCGCGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTaa                                         <  2:1472672/100‑1 (MQ=255)
                                                        gTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAata                                      <  1:465540/100‑1 (MQ=255)
                                                          tCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGGCTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATg                                    >  2:693724/1‑100 (MQ=255)
                                                           cTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGa                                   <  1:2189314/100‑1 (MQ=255)
                                                           cTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGa                                   <  1:1271129/100‑1 (MQ=255)
                                                            tGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGaa                                  <  2:3956537/100‑1 (MQ=255)
                                                             gcgcTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAg                                 >  2:3905264/1‑100 (MQ=255)
                                                             gcgcTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAg                                 >  1:3217083/1‑100 (MQ=255)
                                                              cgcTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGa                                <  1:1829125/100‑1 (MQ=255)
                                                              cgcTCGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGa                                >  2:2347588/1‑100 (MQ=255)
                                                                  cgacGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGaa                            <  1:1745050/97‑1 (MQ=255)
                                                                  cGCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCACATTAAATATGAAGACGaa                            >  1:3931097/1‑100 (MQ=255)
                                                                   gCCGACAACGCGGCTATTGTGTTGTGGGAAGCGCAGACTCAACGTGCCTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGaaa                           <  2:271244/100‑1 (MQ=255)
                                                                   gCCGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGaaa                           >  2:1570314/1‑100 (MQ=255)
                                                                    ccGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAAc                          <  1:549995/100‑1 (MQ=255)
                                                                    ccGACAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAAc                          <  1:3835548/100‑1 (MQ=255)
                                                                        cAACGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGtt                      >  1:917341/1‑100 (MQ=255)
                                                                          aCGCGGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCt                    <  1:3369026/100‑1 (MQ=255)
                                                                          aCGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCt                    <  2:1514341/100‑1 (MQ=255)
                                                                           cgcgGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATCTGAAGACGAAACTGTTCTg                   >  2:1700390/1‑100 (MQ=255)
                                                                           cgcgGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAGGACGAAACTGTTCTg                   >  1:2860524/1‑100 (MQ=255)
                                                                           cgcgGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTg                   >  1:948833/1‑100 (MQ=255)
                                                                           cgcgGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTg                   >  2:875848/1‑100 (MQ=255)
                                                                            gcgGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTgg                  <  2:3444225/100‑1 (MQ=255)
                                                                            gcgGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTgg                  <  1:3498337/100‑1 (MQ=255)
                                                                             cgGCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGc                 <  2:928985/100‑1 (MQ=255)
                                                                              ggCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGca                >  1:1207323/1‑100 (MQ=255)
                                                                              ggCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGca                <  2:118459/100‑1 (MQ=255)
                                                                               gCTATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGcac               >  1:142996/1‑100 (MQ=255)
                                                                                 tATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCacac             <  2:2731366/100‑1 (MQ=255)
                                                                                 tATTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCacac             <  1:3286708/100‑1 (MQ=255)
                                                                                  aTTGTGTTGTGGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACg            >  1:2217552/1‑100 (MQ=255)
                                                                                   ttgtgttgtgGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACgg           >  2:103277/1‑100 (MQ=255)
                                                                                   ttgtgttgtgGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACgg           >  2:2986478/1‑100 (MQ=255)
                                                                                   ttgtgttgtgGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACgg           >  2:2986826/1‑100 (MQ=255)
                                                                                   ttgtgttgtgGCACGCGCAGACCCAGCGTTCCTCTTATTTCGCGTCGCTTGAAAAAGACACCCCCATTAAATATGAAGGCGAAGCTGTTCTGGcaaaggc           <  2:2839930/100‑6 (MQ=255)
                                                                                      tgttgtgGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTcc        >  1:2986874/1‑100 (MQ=255)
                                                                                         tgtgGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGt     <  2:3924108/100‑1 (MQ=255)
                                                                                         tgtgGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGt     <  1:1683755/100‑1 (MQ=255)
                                                                                           tgGCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTAc   <  1:123914/100‑1 (MQ=255)
                                                                                            ggCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGANGAAACTGTTCTGGNACACGGTCCGGTACg  >  2:1944969/1‑100 (MQ=255)
                                                                                            ggCACGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACg  >  2:358086/1‑100 (MQ=255)
                                                                                            ggCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACg  >  2:677145/1‑100 (MQ=255)
                                                                                                |                                                                                               
ATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACG  >  NC_000913/2854400‑2854591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑