BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,957,342	T→G	K515Q (AAG→CAG)	ptrA ←	protease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,957,342	0	T	G	95.5%	508.6 / 8.5	157	K515Q (AAG→CAG)	ptrA	protease III
Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (85/65); total (88/69)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 162 total aligned reads displayed.

TTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAGTTTGTG  >  NC_000913/2957245‑2957437

ttGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGaa                                                                                               <  1:1580455/100‑1 (MQ=255)

  gggCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATc                                                                                             <  2:1757518/100‑1 (MQ=255)

  gggCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATc                                                                                             <  1:2847878/100‑1 (MQ=255)

  gggCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATc                                                                                             >  2:3202528/1‑100 (MQ=255)

       cGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCga                                                                                        >  1:2565246/1‑100 (MQ=255)

       cGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCga                                                                                        <  1:2824760/100‑1 (MQ=255)

        gCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCgag                                                                                       <  2:2229748/100‑1 (MQ=255)

           ggCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGaaa                                                                                    >  1:2148093/1‑100 (MQ=255)

             cAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAatc                                                                                  >  1:2666137/1‑100 (MQ=255)

             cAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAatc                                                                                  <  1:1194240/100‑1 (MQ=255)

             cAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAatc                                                                                  >  2:2708658/1‑100 (MQ=255)

             cAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAatc                                                                                  >  1:2213206/1‑100 (MQ=255)

                  tAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAg                                                                             >  1:474610/1‑100 (MQ=255)

                   aaCGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAgg                                                                            <  2:3961477/100‑1 (MQ=255)

                   aaCGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAgg                                                                            >  2:2616376/1‑100 (MQ=255)

                   aaCGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAgg                                                                            <  1:1521078/100‑1 (MQ=255)

                    aCGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGa                                                                           >  2:2111548/1‑100 (MQ=255)

                     cGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGaa                                                                          <  1:2784179/100‑1 (MQ=255)

                     cGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGaa                                                                          >  1:1057157/1‑100 (MQ=255)

                      ggCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAat                                                                         >  1:969546/1‑100 (MQ=255)

                       gCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAata                                                                        <  2:860444/100‑1 (MQ=255)

                       gCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAata                                                                        <  2:2659434/100‑1 (MQ=255)

                           ggCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAg                                                                    <  2:2114992/100‑1 (MQ=255)

                            gcgcATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAgg                                                                   >  2:1640714/1‑100 (MQ=255)

                            gcgcATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAgg                                                                   >  2:337677/1‑100 (MQ=255)

                              gcATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGt                                                                 >  2:1527717/1‑100 (MQ=255)

                               cATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGtt                                                                <  1:1552704/100‑1 (MQ=255)

                                aTACACCACGCGCAGATTTGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTa                                                               >  1:2973570/1‑100 (MQ=255)

                                aTACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTa                                                               <  2:1665437/100‑1 (MQ=255)

                                  acacCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAg                                                             <  1:2503064/100‑1 (MQ=255)

                                   caccacGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGc                                                            <  2:2637146/100‑1 (MQ=255)

                                   caccacGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGc                                                            >  1:3085355/1‑100 (MQ=255)

                                    accacGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGct                                                           >  1:3453495/1‑100 (MQ=255)

                                    accacGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGct                                                           >  2:2548110/1‑100 (MQ=255)

                                     ccacGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGctc                                                          >  1:1440942/1‑100 (MQ=255)

                                     ccacGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAACGGTTAAGctc                                                          >  2:1507975/1‑100 (MQ=255)

                                        cgcgCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTgg                                                       <  1:722671/100‑1 (MQ=255)

                                         gcgcAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGc                                                      <  1:3690027/100‑1 (MQ=255)

                                           gcAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCaa                                                    >  2:756906/1‑100 (MQ=255)

                                             aGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAag                                                  <  1:826980/100‑1 (MQ=255)

                                             aGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAag                                                  <  1:279010/100‑1 (MQ=255)

                                               aTTCGACTCATCAACAATCAGCTTTGGATGGTCGTATTTCTTCTCGGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAagag                                                <  1:452706/100‑1 (MQ=255)

                                               aTTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAagag                                                >  1:3172179/1‑100 (MQ=255)

                                                 tCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAagagag                                              >  1:2722556/1‑100 (MQ=255)

                                                  cGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAgc                                             >  1:2046399/1‑100 (MQ=255)

                                                  cGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAgc                                             >  2:3725053/1‑100 (MQ=255)

                                                   gACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAgcg                                            <  2:2695639/100‑1 (MQ=255)

                                                     cTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGCATATACGGGTTAAGCTCTGGCAAAGAGAGCGCa                                          >  2:508267/1‑100 (MQ=255)

                                                     cTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCa                                          >  2:3073447/1‑100 (MQ=255)

                                                     cTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCa                                          >  2:659467/1‑100 (MQ=255)

                                                     cTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCa                                          >  2:1155449/1‑100 (MQ=255)

                                                     cTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGCGTTAAGCTCTGGCAAAGAGAGCGCa                                          >  2:1876921/1‑100 (MQ=255)

                                                       catcaACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAAt                                        >  2:1166793/1‑100 (MQ=255)

                                                       catcaACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAAt                                        >  1:2379801/1‑100 (MQ=255)

                                                        atcaACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATg                                       >  1:1677473/1‑100 (MQ=255)

                                                         tcaACAATCAGCGCTGGATGGTCGTATTTCTTCGCTGACTGAATCAGCGAGAAATCATCAGGACTATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGt                                      >  2:1955707/1‑100 (MQ=255)

                                                          caacaaTCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTc                                     >  1:2326556/1‑100 (MQ=255)

                                                          caacaaTCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTc                                     <  2:2163947/100‑1 (MQ=255)

                                                            acaaTCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTcgg                                   >  1:3119659/1‑100 (MQ=255)

                                                            acaaTCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTcgg                                   >  1:1863280/1‑100 (MQ=255)

                                                                tCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCggcggc                               <  1:3194089/100‑1 (MQ=255)

                                                                tCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCggcggc                               <  2:3111873/100‑1 (MQ=255)

                                                                tCAGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCggcggc                               <  2:2802412/100‑1 (MQ=255)

                                                                  aGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCtt                             >  1:2328508/1‑100 (MQ=255)

                                                                  aGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCtt                             >  2:3473902/1‑100 (MQ=255)

                                                                  aGCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCtt                             <  2:2929166/100‑1 (MQ=255)

                                                                   gCTCTGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCttt                            >  1:3682911/1‑100 (MQ=255)

                                                                    cgatGGATGGTCGTATTTCTTATCGGACTTAATCAGCGAGAAATCAGCAGGAATATAAGGGTTAAGGTCTGGCGAAGAGAGCGCAATGTCGGCGGCtttt                           <  2:3283097/97‑1 (MQ=255)

                                                                     tctGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCttttt                          <  1:3939118/100‑1 (MQ=255)

                                                                     tctGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCttttt                          >  2:3946009/1‑100 (MQ=255)

                                                                     tctGGATGGTCGTATGTCTTCGCTGACTGAATCAGCGAGAAATCATCAGGAGTATAAGGGTTAAGCTCTGGCAAGGCGAGTGCAATGTCGGCGGCttttt                          <  2:1363043/100‑1 (MQ=255)

                                                                       tGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTc                        <  2:551475/100‑1 (MQ=255)

                                                                       tGGATGGTCGTATTTCTTCTCTGACTGAATCAGCGAAAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTc                        >  2:1198188/1‑100 (MQ=255)

                                                                        ggATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCt                       >  2:2944357/1‑100 (MQ=255)

                                                                         gATGGTTGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTg                      <  2:228031/100‑1 (MQ=255)

                                                                         gATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCCAAGAGAGCGCAATGTCGGCGGCTTTTTTCTg                      >  2:2097084/1‑100 (MQ=255)

                                                                         gATGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTg                      <  1:460216/100‑1 (MQ=255)

                                                                          aTGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGc                     <  1:607279/100‑1 (MQ=255)

                                                                           tGGTCGTATTTTTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATACGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGcc                    <  1:1841055/100‑1 (MQ=255)

                                                                           tGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGcc                    <  1:1076795/100‑1 (MQ=255)

                                                                           tGGTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGcc                    >  2:1984052/1‑100 (MQ=255)

                                                                             gTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCGAg                  >  1:2630426/1‑100 (MQ=255)

                                                                             gTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAg                  >  1:1953641/1‑100 (MQ=255)

                                                                             gTCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAg                  <  2:35334/100‑1 (MQ=255)

                                                                              tCGTATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGt                 <  1:1985029/100‑1 (MQ=255)

                                                                                 tATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCgg              <  1:3089920/100‑1 (MQ=255)

                                                                                 tATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCgg              <  1:1556884/100‑1 (MQ=255)

                                                                                 tATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCgg              <  2:376625/100‑1 (MQ=255)

                                                                                 tATTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCgg              >  2:3030155/1‑100 (MQ=255)

                                                                                  aTTTCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGc             <  1:3900607/100‑1 (MQ=255)

                                                                                   tttCTTCTCTGACTGAATCAGCGATAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGATCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCg            <  2:3697366/100‑1 (MQ=255)

                                                                                   tttCTTCTCTGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCg            >  1:3624488/1‑100 (MQ=255)

                                                                                       cgatctGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAg        <  1:2289822/97‑1 (MQ=255)

                                                                                          tctGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAGttt     >  2:1305877/1‑100 (MQ=255)

                                                                                           ctgactgaATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAGTTtg    <  2:3826069/100‑1 (MQ=255)

                                                                                           ctgactgaATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAGTTtg    >  1:3500690/1‑100 (MQ=255)

                                                                                           ctgactgaATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGACAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCCGTCGGCGAACGTTtg    >  2:2848622/1‑100 (MQ=255)

                                                                                            tGACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAGTTtgt   >  2:682077/1‑100 (MQ=255)

                                                                                             gACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAGTTtgtg  <  2:1844512/100‑1 (MQ=255)

                                                                                             gACTGAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAGTTtgtg  <  1:1178167/100‑1 (MQ=255)

TTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAGAGAGCGCAATGTCGGCGGCTTTTTTCTGCCAGTCGGCGAAAGTTTGTG  >  NC_000913/2957245‑2957437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`