Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,007,340 A→C E360A (GAA→GCA)  ygeW → putative carbamoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,007,3400AC98.4% 418.3 / ‑1.6 123E360A (GAA→GCA) ygeWputative carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (75/46);  total (76/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

Only 100 of 131 total aligned reads displayed.

ATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCA  >  NC_000913/3007243‑3007436
                                                                                                 |                                                                                                
aTGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAaagcag                                                                                                <  2:1095628/100‑1 (MQ=255)
 tGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAGGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAagcagc                                                                                               >  2:1762345/1‑100 (MQ=255)
 tGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAagcagc                                                                                               >  1:2037620/1‑100 (MQ=255)
  gCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGcc                                                                                              <  1:1382796/100‑1 (MQ=255)
   cACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAATGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCa                                                                                             <  2:2621915/100‑1 (MQ=255)
   cACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCa                                                                                             >  1:2337500/1‑100 (MQ=255)
   cACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGCGAAGTGACTGAAGGCGTATTCGAAAAAAACCGTATCGCTGCCTACAAAGCAGccc                                                                                             >  2:1886960/1‑99 (MQ=255)
     ctgcctgcCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGcagccagc                                                                                           >  1:68975/1‑100 (MQ=255)
     ctgcctgcCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGcagccagc                                                                                           <  1:1569008/100‑1 (MQ=255)
     ctgcctgcCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGcagccagc                                                                                           <  1:2860649/100‑1 (MQ=255)
     ctgcctgcCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGcagccagc                                                                                           <  1:2532480/100‑1 (MQ=255)
       gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTg                                                                                         >  2:3068491/1‑100 (MQ=255)
       gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTg                                                                                         <  2:2204156/100‑1 (MQ=255)
        cctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTgg                                                                                        >  2:1240719/1‑100 (MQ=255)
        cctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTgg                                                                                        <  1:2586449/100‑1 (MQ=255)
          tgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGaa                                                                                      <  1:642636/100‑1 (MQ=255)
          tgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGaa                                                                                      >  1:975318/1‑100 (MQ=255)
          tgccAGCTGATATCAGCGGCGTACCCTGTAAAGAAGGTGAAGTGACTGAAGGCATATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGaa                                                                                      <  2:3116776/100‑1 (MQ=255)
           gccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAg                                                                                     >  1:521181/1‑100 (MQ=255)
              aGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCt                                                                                  >  1:2625816/1‑100 (MQ=255)
               gCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCtt                                                                                 <  2:3546139/100‑1 (MQ=255)
                cTGATATCAGCGGTGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTCTTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTta                                                                                <  1:3677851/100‑1 (MQ=255)
                cTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTta                                                                                <  2:3575323/100‑1 (MQ=255)
                cTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTta                                                                                >  2:2066762/1‑100 (MQ=255)
                 taatatCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAAGCGTAATCGAAAAATACCGGATCGCTACCTACGAAGCAGCGAGCTGGAAGCCTtat                                                                               <  2:390286/98‑1 (MQ=255)
                  gATATCGGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTtata                                                                              <  2:1733625/100‑1 (MQ=255)
                  gATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTtata                                                                              <  2:3466806/100‑1 (MQ=255)
                    tatCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATatc                                                                            >  1:72977/1‑100 (MQ=255)
                    tatCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATatc                                                                            >  1:196903/1‑100 (MQ=255)
                      tCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATatcat                                                                          >  2:3261868/1‑100 (MQ=255)
                      tCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATatcat                                                                          >  2:2802459/1‑100 (MQ=255)
                        aGCGGCGTATCCTGTAAAGAAGGTGAAGTGGCTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATcg                                                                        <  1:1840888/100‑1 (MQ=255)
                        aGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATcg                                                                        >  1:2020793/1‑100 (MQ=255)
                        aGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATcg                                                                        >  1:3321345/1‑100 (MQ=255)
                        aGCCGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATcg                                                                        >  1:3142945/1‑100 (MQ=255)
                         gcggcgTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATcgc                                                                       >  1:2875145/1‑100 (MQ=255)
                         gcggcgTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATcgc                                                                       >  1:1212335/1‑100 (MQ=255)
                                aTCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTCTATCATCGCCGCgatg                                                                >  2:2356436/1‑100 (MQ=255)
                                aTCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCgatg                                                                <  1:445927/100‑1 (MQ=255)
                                  ccTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCgatgat                                                              >  1:1424201/1‑100 (MQ=255)
                                  ccTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCgatgat                                                              >  2:672424/1‑100 (MQ=255)
                                  ccTGTAAAGAAGGGGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCgatgat                                                              >  1:1338295/1‑100 (MQ=255)
                                   cTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATc                                                             >  2:1190267/1‑100 (MQ=255)
                                   cTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATc                                                             >  2:725550/1‑100 (MQ=255)
                                    tGTAAAGAAGGTGAAGTGACTGAAAAAAAAAAAAAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATcc                                                            <  2:2466220/100‑1 (MQ=255)
                                     gTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCt                                                           >  2:754535/1‑100 (MQ=255)
                                     gTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCt                                                           >  2:824614/1‑100 (MQ=255)
                                     gTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGAATCGCTACCTACAAAGCAGCCAGTTGGAAGCCTTATATCATCGCCGCGATGATccc                                                           >  1:1370769/1‑99 (MQ=255)
                                       aaaGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGt                                                         >  2:2762043/1‑100 (MQ=255)
                                       aaaGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGt                                                         >  2:475687/1‑100 (MQ=255)
                                        aagaagGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTc                                                        >  2:1077404/1‑100 (MQ=255)
                                          gaagGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTccc                                                      <  1:500631/100‑1 (MQ=255)
                                           aagGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATGCTGTCCCg                                                     <  2:3832065/100‑1 (MQ=255)
                                           aagGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCg                                                     >  1:1676308/1‑100 (MQ=255)
                                           aagGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCg                                                     <  1:759463/100‑1 (MQ=255)
                                            agGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGt                                                    >  2:846307/1‑100 (MQ=255)
                                             ggTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTa                                                   >  1:670095/1‑100 (MQ=255)
                                             ggTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTa                                                   >  2:843445/1‑100 (MQ=255)
                                                  aGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATAc                                              <  1:1190731/100‑1 (MQ=255)
                                                    tGACTGGAGGCGTATTCGAAAAATACCGTATCGCTTCCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGc                                            <  2:3371886/100‑1 (MQ=255)
                                                     gACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGcc                                           <  1:2339712/100‑1 (MQ=255)
                                                       cTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCaa                                         >  1:53689/1‑100 (MQ=255)
                                                       cTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCaa                                         >  1:3434626/1‑100 (MQ=255)
                                                         gAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAAc                                       >  2:1174478/1‑100 (MQ=255)
                                                           tggCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCa                                     <  1:1575297/99‑1 (MQ=255)
                                                           tggCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCCGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAAccn                                     <  1:300536/99‑2 (MQ=255)
                                                           aGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCa                                     <  2:3901496/100‑1 (MQ=255)
                                                            ggCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAg                                    <  1:247925/100‑1 (MQ=255)
                                                            ggCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAg                                    >  1:2640602/1‑100 (MQ=255)
                                                             gCGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAgg                                   <  1:308119/100‑1 (MQ=255)
                                                              cGTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCTGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGt                                  >  1:3841699/1‑100 (MQ=255)
                                                               gTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTg                                 >  2:2372487/1‑100 (MQ=255)
                                                               gTATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGAACCTGTCCCGTAAATACGCCAAACCAGGTg                                 >  1:630323/1‑100 (MQ=255)
                                                                tATTCGAAAAATACCGTATCGCTACCTACAAGGCAGCCAGCGGGAAGCCTTATATCATCGCCGCGATGATACTCTCCCGTAAATACGCCAAACCAGGTGc                                >  2:3803875/1‑100 (MQ=255)
                                                                tATTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGc                                <  2:1210976/100‑1 (MQ=255)
                                                                 aTTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGCTCCTGTCCAGTAAATACGCCAAACCAGGGGCa                               >  2:2102796/1‑100 (MQ=255)
                                                                 aTTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCa                               >  1:3927953/1‑100 (MQ=255)
                                                                 aTTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCa                               >  1:1212223/1‑100 (MQ=255)
                                                                 aTTCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCa                               >  1:1742649/1‑100 (MQ=255)
                                                                  ttCGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCAc                              >  2:1646496/1‑100 (MQ=255)
                                                                    cGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTg                            >  1:1194093/1‑100 (MQ=255)
                                                                    cGAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACCCCAAACCAGGTGCACTg                            >  2:3489579/1‑100 (MQ=255)
                                                                     gAAAAATACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTGATATCATCGCCGCGATGATCCTGTCCCTTAAATACGCCAGACCAGGTGCACTGc                           <  2:3736535/100‑1 (MQ=255)
                                                                      aaaaaTACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTTTCCCGTAAATACGCCAAACCAGGTGCACTGCt                          >  2:2737397/1‑100 (MQ=255)
                                                                      aaaaaTACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCAACGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCt                          >  1:3888500/1‑100 (MQ=255)
                                                                          aTACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAg                      <  1:2871125/100‑1 (MQ=255)
                                                                          aTACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAg                      <  1:2223000/100‑1 (MQ=255)
                                                                          aTACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAg                      <  2:2005727/100‑1 (MQ=255)
                                                                           tACCGTATCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGc                     >  2:1825665/1‑100 (MQ=255)
                                                                                 aTCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgc               >  2:1440787/1‑100 (MQ=255)
                                                                                 aTCGCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgc               <  1:2393187/100‑1 (MQ=255)
                                                                                    gCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGa            >  2:1563318/1‑100 (MQ=255)
                                                                                    gCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGa            >  1:1274996/1‑100 (MQ=255)
                                                                                    gCTACCTACAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCCGa            <  2:3807100/100‑1 (MQ=255)
                                                                                      tacctacAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGaaa          >  1:2424937/1‑100 (MQ=255)
                                                                                         ctacAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaagaa       >  1:2027884/1‑100 (MQ=255)
                                                                                           acAAAGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgc     >  1:140504/1‑100 (MQ=255)
                                                                                             aaaGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgcgc   >  2:1443373/1‑100 (MQ=255)
                                                                                             aaaGCAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgcgc   <  2:3367342/100‑1 (MQ=255)
                                                                                              aagcagCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCa  <  1:1842306/100‑1 (MQ=255)
                                                                                                 |                                                                                                
ATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCA  >  NC_000913/3007243‑3007436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: