Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,014,506 T→G Q184H (CAA→CAC yqeC ← putative selenium‑dependent hydroxylase accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,014,5060TG99.3% 495.8 / ‑5.1 148Q184H (CAA→CACyqeCputative selenium‑dependent hydroxylase accessory protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (67/80);  total (67/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 148 total aligned reads displayed.

GAGAAAAACGGTTAATGAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGTTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATG  >  NC_000913/3014409‑3014598
                                                                                                 |                                                                                            
 agaAAAACGGTTAATGAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGc                                                                                           >  1:560120/1‑100 (MQ=255)
 agaAAAACGGTTAATGAACCAGACTCGCCGACAACCTTGCGGTACGGTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCCGGTGc                                                                                           >  1:868898/1‑100 (MQ=255)
 agaAAAACGGTTAATGAACAAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGc                                                                                           >  2:3565769/1‑100 (MQ=255)
  gaAAAACGGTTAATGAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCa                                                                                          >  2:2535302/1‑100 (MQ=255)
    aaaaCGGTTAATGAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAg                                                                                        >  2:519448/1‑100 (MQ=255)
     aaaCGGTTAATGAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTTAGGTGCAAgg                                                                                       <  1:62035/100‑1 (MQ=255)
        cGGTTAATGAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCCAAGGTGCAAGGTTg                                                                                    >  2:3435823/1‑100 (MQ=255)
         ggTTAATGAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGttcc                                                                                   >  1:4061264/1‑98 (MQ=255)
         ggTTAATGAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGc                                                                                   <  1:907902/100‑1 (MQ=255)
          gTTAATGAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCa                                                                                  <  2:719818/100‑1 (MQ=255)
            tAATGAACCAGACTCGCCGACAACCTTGCGGCACGTCTTTAAACGCCCCCTGAGGGTGGCGAACAAGCGCCACGAGATCGCTCAGGTGAAAGGTTGCATc                                                                                >  2:144262/1‑100 (MQ=255)
              gggAACCAGACTCGCCGACGACCTTGCGGTACGTTTTTAGACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAg                                                                              <  2:2320025/98‑1 (MQ=255)
              aTGAACCAGACTTGCGCACAACCCTGCGGTACCTTTTTAAACGCACCCGGAGGGTGGCGAACCAGCGCAACGAGATCGGTCAGGCGCGAGGTGGCATAAg                                                                              >  2:2457575/1‑100 (MQ=255)
               tGAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAgg                                                                             <  1:632953/100‑1 (MQ=255)
                gAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTCCATCAGgt                                                                            >  1:3120272/1‑100 (MQ=255)
                 aaCCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGTTGCAAGGTTGCATCAGgtg                                                                           <  2:1107751/100‑1 (MQ=255)
                 aaCCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGgtg                                                                           >  2:1328856/1‑100 (MQ=255)
                  aCCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGgtgt                                                                          >  2:2142031/1‑100 (MQ=255)
                  aCCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGgtgt                                                                          <  2:3483920/100‑1 (MQ=255)
                   ccAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGtt                                                                         <  1:3058305/100‑1 (MQ=255)
                    cAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTa                                                                        <  2:2163650/100‑1 (MQ=255)
                    cAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTa                                                                        >  2:395081/1‑100 (MQ=255)
                      gACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAAc                                                                      <  1:2666652/100‑1 (MQ=255)
                       aCTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAAcc                                                                     <  2:3798330/100‑1 (MQ=255)
                        cTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAAccc                                                                    >  1:2516396/1‑100 (MQ=255)
                        cTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAAccc                                                                    >  2:2592475/1‑100 (MQ=255)
                          cGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCACCGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCgg                                                                  <  1:3470623/100‑1 (MQ=255)
                          cGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCgg                                                                  <  2:2804339/100‑1 (MQ=255)
                            ccGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTa                                                                <  1:538934/100‑1 (MQ=255)
                              gACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAat                                                              <  1:2927212/100‑1 (MQ=255)
                              gACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAat                                                              <  1:2050359/100‑1 (MQ=255)
                                cAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCACGTCTTACCCCCGTAatag                                                            >  1:1992504/1‑99 (MQ=255)
                                 aaCCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATc                                                           <  2:1154125/100‑1 (MQ=255)
                                 aaCCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATc                                                           <  1:750959/100‑1 (MQ=255)
                                 aaCCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATc                                                           <  2:176705/100‑1 (MQ=255)
                                  aCCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCCCAGGGGCAAGGTTGCATCAGGCGTTAACCCGGTAATATcg                                                          >  1:375075/1‑99 (MQ=255)
                                   ccgtGCGGTACGTTTTGAAACGCCGCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAg                                                         <  2:1226546/97‑1 (MQ=255)
                                   ccTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAg                                                         >  2:228575/1‑100 (MQ=255)
                                    cTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGc                                                        <  2:904880/100‑1 (MQ=255)
                                        cGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAAc                                                    <  1:2162738/100‑1 (MQ=255)
                                          gTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTg                                                  <  1:1875557/100‑1 (MQ=255)
                                             cGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATCTCCGCCCACTGCGa                                               >  1:3041307/1‑100 (MQ=255)
                                             cGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGa                                               >  1:1790713/1‑100 (MQ=255)
                                             cGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGACTCAACCCGGTAATATCCGCATACGGctc                                               >  1:1505/1‑98 (MQ=255)
                                              gTTTTTAAACGCCCCCTGAGGGAGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGAc                                              >  2:77539/1‑100 (MQ=255)
                                               tttttAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGAcc                                             <  2:518678/100‑1 (MQ=255)
                                                ttttAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCa                                            >  2:410054/1‑100 (MQ=255)
                                                ttttAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCa                                            >  2:1548097/1‑100 (MQ=255)
                                                 tttAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAg                                           >  2:1328542/1‑100 (MQ=255)
                                                    aaaCGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGa                                        >  2:1214013/1‑100 (MQ=255)
                                                    aaaCACCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGGTGCATCAGGTGATAACCCGGTAATATCAGCAAACTGCGACCAGCGa                                        >  1:4061579/1‑100 (MQ=255)
                                                       gccacccTCAGGGGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGa                                    >  2:1900777/5‑100 (MQ=255)
                                                       cGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATgg                                     >  2:2067772/1‑100 (MQ=255)
                                                       cGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGCTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATgg                                     >  2:3331048/1‑100 (MQ=255)
                                                         cccccTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGAc                                   <  2:392155/100‑1 (MQ=255)
                                                           cccTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTGCGCCGGTAATATCAGCCCACTCCTTCCAGCCATGGCCAt                                 >  1:689676/1‑100 (MQ=255)
                                                           cccTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTCAACCCGGTAATATCATCCCACTCCGACCAGCTACGGCCAt                                 >  1:81285/1‑100 (MQ=255)
                                                             cTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACAttt                               <  1:3548277/100‑1 (MQ=255)
                                                              tGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACAtttt                              <  1:3742529/100‑1 (MQ=255)
                                                               gAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTc                             <  1:1138428/100‑1 (MQ=255)
                                                               gAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTc                             >  2:3842092/1‑100 (MQ=255)
                                                                 gggTGGCGAACCAGCGCAACTAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCtg                           <  1:1009056/100‑1 (MQ=255)
                                                                 gggTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGCCATTTTCtg                           >  1:3986691/1‑100 (MQ=255)
                                                                 gggTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCtg                           <  1:1927839/100‑1 (MQ=255)
                                                                 gggTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCtg                           <  1:351391/100‑1 (MQ=255)
                                                                 gggTGGCGAACCAGCGCAACGAGATCGCTCAGGCGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCtg                           >  2:2433782/1‑100 (MQ=255)
                                                                  ggTGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCtgt                          <  2:310268/100‑1 (MQ=255)
                                                                   gTGGCGAGCCAGCGCGACGAGATCGATCAGGTGCAATGTTGCATCAGGTGTTAACCCGGTAATTTCTGCAAACTGCGACCAGCGATGTACATTTTCcgtg                         <  2:873921/100‑1 (MQ=255)
                                                                    tGGCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGc                        <  1:2515772/100‑1 (MQ=255)
                                                                     ggCGAACCGGCGCAACGAGCTCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCt                       <  2:3572547/100‑1 (MQ=255)
                                                                     ggCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAAGCCGGTAATATCAGCAAACTGCGACCAGCGATGGACGTTTGCTGTGCt                       >  1:2880717/1‑100 (MQ=255)
                                                                     ggCGAACCAGCGCAACGAGATCGCTCAGGCGCAAGGTTGCAACAGGTGTGAACCCGGTAATATCAGCAAACTTGGAGCAGCGATTGACATCTTCTGTACt                       >  1:2399280/1‑100 (MQ=255)
                                                                      gCGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTc                      >  2:2997856/1‑100 (MQ=255)
                                                                       cGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCa                     >  2:3960633/1‑100 (MQ=255)
                                                                       cGAACCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGACGGACATTTTCTGTGCTCa                     >  1:1367575/1‑100 (MQ=255)
                                                                         aaCCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACt                   <  1:167460/100‑1 (MQ=255)
                                                                         aaCCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACt                   <  1:2007297/100‑1 (MQ=255)
                                                                         aaCCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACt                   <  2:1577948/100‑1 (MQ=255)
                                                                         aaCCAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACCGCGACCAGCGATGGACATTTTCTGTGCTCACt                   <  2:42115/100‑1 (MQ=255)
                                                                           ccAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACttt                 >  1:2904885/1‑100 (MQ=255)
                                                                           ccAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACttt                 <  2:3762594/100‑1 (MQ=255)
                                                                            cAGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTc                <  2:1791163/100‑1 (MQ=255)
                                                                             aGCGCAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTcg               <  1:1121650/100‑1 (MQ=255)
                                                                               cgcAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTcgcg             <  1:606875/100‑1 (MQ=255)
                                                                               cgcAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTcgcg             <  2:2258465/100‑1 (MQ=255)
                                                                               cgcAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTcgcg             <  2:3049148/100‑1 (MQ=255)
                                                                                 cAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGcc           >  1:273811/1‑100 (MQ=255)
                                                                                 cAACGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGcc           >  2:2875781/1‑100 (MQ=255)
                                                                                  aaCGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGccc          <  2:1083859/100‑1 (MQ=255)
                                                                                  aaCGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGccc          <  1:232291/100‑1 (MQ=255)
                                                                                   aCGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCa         >  1:2592177/1‑100 (MQ=255)
                                                                                   aCGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCa         >  2:3259718/1‑100 (MQ=255)
                                                                                   aCGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCa         <  1:3973503/100‑1 (MQ=255)
                                                                                    cGAGATCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCaa        <  2:2455231/100‑1 (MQ=255)
                                                                                        aTCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTa    <  1:3314116/100‑1 (MQ=255)
                                                                                        aTCGCTCAGGTGCAAGGTTACATCATGTCTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGTACATTTTCTGTGCTCACTTTCGCGCCCAAAGTa    <  2:275706/100‑1 (MQ=255)
                                                                                         tCGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTAt   <  2:2248504/100‑1 (MQ=255)
                                                                                          cGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATg  >  2:1461385/1‑100 (MQ=255)
                                                                                          cGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATg  <  1:1301252/100‑1 (MQ=255)
                                                                                          cGCTCAGGTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATg  <  1:1851393/100‑1 (MQ=255)
                                                                                                 |                                                                                            
GAGAAAAACGGTTAATGAACCAGACTCGCCGACAACCTTGCGGTACGTTTTTAAACGCCCCCTGAGGGTGGCGAACCAGCGCAACGAGATCGCTCAGTTGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATG  >  NC_000913/3014409‑3014598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: