Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,044,628 A→C K323Q (AAG→CAG)  bglA → 6‑phospho‑beta‑glucosidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,044,6280AC96.0% 493.3 / 8.1 150K323Q (AAG→CAG) bglA6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base C (50/94);  total (53/97)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

Only 100 of 154 total aligned reads displayed.

CGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATC  >  NC_000913/3044531‑3044724
                                                                                                 |                                                                                                
cgGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAAtgcagtgcag                                                                                                >  2:1632823/1‑100 (MQ=255)
cgGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAAtgcagtgcag                                                                                                <  1:4026059/100‑1 (MQ=255)
 ggATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAgg                                                                                               <  2:204381/100‑1 (MQ=255)
  gATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGc                                                                                              <  1:1535774/100‑1 (MQ=255)
   aTTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGcc                                                                                             <  1:3824516/100‑1 (MQ=255)
   aTTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGcc                                                                                             <  2:1184865/100‑1 (MQ=255)
    tttAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCg                                                                                            <  2:3328582/100‑1 (MQ=255)
       aaCATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAg                                                                                         >  1:1774049/1‑100 (MQ=255)
       aaCATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAg                                                                                         <  2:3842584/100‑1 (MQ=255)
        aCATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAgg                                                                                        >  1:2877494/1‑100 (MQ=255)
         cATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggc                                                                                       >  2:2679009/1‑100 (MQ=255)
         cATCAAAATGGAAGACGGCGATCGGGATGTGCTGTGTGAAGGCACCTGCGATGATCTTGGTTTCAGCGATTACATGACCAATGCAGTGCAGGCCGAAggc                                                                                       >  2:3024787/1‑100 (MQ=255)
          aTCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggcg                                                                                      >  2:2481170/1‑100 (MQ=255)
          aTCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggcg                                                                                      >  1:1344588/1‑100 (MQ=255)
          aTCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATAACCAATGCAGTGCAGGCCGAAggcg                                                                                      <  2:1218391/100‑1 (MQ=255)
           tCAAAATGGAAGACGGCGATCTGGATGTGCGGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggcgg                                                                                     >  2:280664/1‑100 (MQ=255)
           tCAAAAGGGAAGACGGCGATCTGGATGTGCGGCGTGGAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggcgg                                                                                     <  1:3836989/100‑1 (MQ=255)
            cAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAggcggc                                                                                    <  2:157509/100‑1 (MQ=255)
             aaaaTGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCa                                                                                   <  2:1988011/100‑1 (MQ=255)
               aaTGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCAcc                                                                                 <  1:2652336/100‑1 (MQ=255)
                 tGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCgg                                                                               >  2:1016216/1‑100 (MQ=255)
                 tGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCgg                                                                               <  2:732045/100‑1 (MQ=255)
                  ggAAGACGGCGATCTGGATGTACTGCCTGAAGGCACCTGTGATTATCTTGGTTTCAGCTATTACATGACCGATGCAGTGCAGGCCGAAGGCGGCACCGgc                                                                              <  1:3809081/100‑1 (MQ=255)
                   gAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcg                                                                             >  2:3473872/1‑100 (MQ=255)
                     aGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgat                                                                           <  2:10767/100‑1 (MQ=255)
                      gACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatg                                                                          <  2:121578/100‑1 (MQ=255)
                      gACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatg                                                                          <  2:3403998/100‑1 (MQ=255)
                        cGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgcg                                                                        >  1:1255493/1‑100 (MQ=255)
                        cGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgcg                                                                        <  1:3680675/100‑1 (MQ=255)
                          gCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgcgat                                                                      <  1:1487833/100‑1 (MQ=255)
                          gCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGgcgatgcgat                                                                      <  2:726055/100‑1 (MQ=255)
                           cGATCTGGATGTGCTGCGTGCAGGCACCTGCGATTATCTTGGTTTCAGCTATTACCTGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGAtc                                                                     <  2:800085/100‑1 (MQ=255)
                           cGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGAtc                                                                     >  1:474822/1‑100 (MQ=255)
                            gATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGAtct                                                                    <  1:1223848/100‑1 (MQ=255)
                             aTCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGAtctc                                                                   <  2:1812771/100‑1 (MQ=255)
                               cTGGATGTGCTGCGTGAAGGCACCTGCGTTTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCTCCGGCGATGCGATCTCTg                                                                 >  2:1144697/1‑100 (MQ=255)
                               cTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTg                                                                 <  1:2375289/100‑1 (MQ=255)
                                tGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTgg                                                                <  1:1527984/100‑1 (MQ=255)
                                 ggATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGt                                                               >  2:2282854/1‑100 (MQ=255)
                                   aTGTGCTGCGTGAAGGGACTTGCGATTGTCTTGGTTTCAGGTAGTACATGACCAATGTTGTGCAGGCCGAAGGGGGCACCGGCGATGCGATCTCTGGttt                                                             <  2:1352347/100‑1 (MQ=255)
                                   aTGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGttt                                                             <  1:3722793/100‑1 (MQ=255)
                                     gtgCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTg                                                           <  1:1693322/100‑1 (MQ=255)
                                       gttgcGTGAAGGAATCAGCGATTATCTTGGTTTCAGCTATTACATGACCTATGCAGTGCAGCCCGAAGGCGGCGCCGGGGATGCGATCTCTGGTTTTTaa                                                         <  2:1696105/98‑1 (MQ=255)
                                       gctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGCAGGCGGCACCGGCGATGCGATCTCTGGTTTTGaa                                                         >  2:2364754/1‑100 (MQ=255)
                                        ctgcGTGAAGGCACCTGCGATTATCTTGGTTTCTGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAg                                                        <  1:4040740/100‑1 (MQ=255)
                                        ctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAg                                                        >  1:3952579/1‑100 (MQ=255)
                                        ctgcGTGAAGGCACCTGCCACTATCTCGGTTTCAGCTATGACATGGCCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAg                                                        <  1:3821083/100‑1 (MQ=255)
                                         tgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTCCATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAgg                                                       <  2:2004001/100‑1 (MQ=255)
                                          gcGTGAACGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGc                                                      <  1:2020350/100‑1 (MQ=255)
                                           cGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCa                                                     <  1:1795878/100‑1 (MQ=255)
                                            gTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAg                                                    <  2:3246547/100‑1 (MQ=255)
                                            gTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAg                                                    >  2:932404/1‑100 (MQ=255)
                                            gTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAg                                                    <  1:998928/100‑1 (MQ=255)
                                              gAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCg                                                  >  2:425293/1‑100 (MQ=255)
                                               aaGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGt                                                 >  2:2104613/1‑100 (MQ=255)
                                                 ggCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTAc                                               <  1:1215830/100‑1 (MQ=255)
                                                 ggCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTAc                                               >  1:1559520/1‑100 (MQ=255)
                                                 ggCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTAc                                               <  1:3520471/100‑1 (MQ=255)
                                                  gCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTAcc                                              <  1:1959894/100‑1 (MQ=255)
                                                  gCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTAcc                                              <  1:3433125/100‑1 (MQ=255)
                                                   cACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCa                                             >  1:2635686/1‑100 (MQ=255)
                                                     ccTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCaaa                                           >  1:2050347/1‑100 (MQ=255)
                                                     ccTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCaaa                                           >  1:297382/1‑100 (MQ=255)
                                                      cTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAAc                                          <  1:1631787/100‑1 (MQ=255)
                                                       tGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAAcc                                         <  2:1445763/100‑1 (MQ=255)
                                                       tGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAAcc                                         >  1:2848642/1‑100 (MQ=255)
                                                         cGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCg                                       >  1:1498961/1‑100 (MQ=255)
                                                            ttATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTAt                                    <  2:1532962/100‑1 (MQ=255)
                                                            ttATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTAt                                    <  1:2357153/100‑1 (MQ=255)
                                                            ttATCTTGGTTTCAGCTATGACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTAt                                    <  2:1797193/100‑1 (MQ=255)
                                                             tATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATg                                   >  1:703858/1‑100 (MQ=255)
                                                             tATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATg                                   <  2:1396905/100‑1 (MQ=255)
                                                              aTCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGt                                  >  1:2055647/1‑100 (MQ=255)
                                                                cTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTa                                <  1:3081413/100‑1 (MQ=255)
                                                                  tGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTaaa                              >  2:287638/1‑100 (MQ=255)
                                                                  tGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTaaa                              >  2:2547277/1‑100 (MQ=255)
                                                                   ggTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAg                             <  2:1109626/100‑1 (MQ=255)
                                                                   ggTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAg                             <  2:803971/100‑1 (MQ=255)
                                                                     tttCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCa                           <  2:225643/100‑1 (MQ=255)
                                                                        cAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCt                        <  2:2431462/100‑1 (MQ=255)
                                                                        cAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCt                        <  1:2419602/100‑1 (MQ=255)
                                                                         aGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTg                       <  1:931531/100‑1 (MQ=255)
                                                                           cTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGAc                     <  1:1955902/100‑1 (MQ=255)
                                                                           cTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGAc                     <  1:1897714/100‑1 (MQ=255)
                                                                              ttACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTgg                  >  1:1446690/1‑100 (MQ=255)
                                                                              ttACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTgg                  <  1:1447019/100‑1 (MQ=255)
                                                                               tACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAATGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTggg                 <  2:3435517/100‑1 (MQ=255)
                                                                               tACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTggg                 <  2:3508876/100‑1 (MQ=255)
                                                                                aCATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTgggg                >  1:2229845/1‑100 (MQ=255)
                                                                                aCATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTgggg                <  1:885980/100‑1 (MQ=255)
                                                                                aCATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTgggg                >  2:2527122/1‑100 (MQ=255)
                                                                                 cATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGc               <  1:2172352/100‑1 (MQ=255)
                                                                                    gACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTgg            >  1:2600809/1‑100 (MQ=255)
                                                                                     aCCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGc           <  2:689126/100‑1 (MQ=255)
                                                                                      ccAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCa          <  1:1605879/100‑1 (MQ=255)
                                                                                         aTGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGAt       <  1:1565759/100‑1 (MQ=255)
                                                                                             aGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGAt   <  1:2582458/100‑1 (MQ=255)
                                                                                              gTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATc  >  1:455138/1‑100 (MQ=255)
                                                                                              gTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATc  <  2:295241/100‑1 (MQ=255)
                                                                                              gTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATc  >  2:2734494/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATC  >  NC_000913/3044531‑3044724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: