Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,056,034 A→C noncoding (52/183 nt) ssrS → 6S RNA inhibits RNA polymerase promoter binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,056,0340AC90.1% 521.9 / 33.8 182noncoding (52/183 nt)ssrS6S RNA inhibits RNA polymerase promoter binding
Reads supporting (aligned to +/- strand):  ref base A (13/5);  new base C (81/83);  total (94/88)
Fisher's exact test for biased strand distribution p-value = 8.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

ATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATACCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCTTGAACCAAGGGT  >  NC_000913/3055938‑3056128
                                                                                                |                                                                                              
aTGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTGTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCca                                                                                             <  2:3934502/100‑1 (MQ=255)
aTGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCca                                                                                             <  1:3138355/100‑1 (MQ=255)
 tGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCcac                                                                                            >  1:3702439/1‑100 (MQ=255)
 tGACACTTTTCGGTTTACGGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCcac                                                                                            <  2:3542891/100‑1 (MQ=255)
   acacTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACaa                                                                                          >  1:375001/1‑100 (MQ=255)
   acacTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACaa                                                                                          >  2:2676219/1‑100 (MQ=255)
      cTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGaa                                                                                       >  2:3318906/1‑100 (MQ=255)
        tttCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAAtg                                                                                     >  1:143723/1‑100 (MQ=255)
        tttCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAAtg                                                                                     <  1:1609592/100‑1 (MQ=255)
           cgttttACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTgg                                                                                  <  2:1826431/97‑1 (MQ=255)
           cGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTgg                                                                                  <  1:401336/100‑1 (MQ=255)
           cGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTgg                                                                                  <  1:2792777/100‑1 (MQ=255)
           cGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTgg                                                                                  <  1:3128083/100‑1 (MQ=255)
            ggTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGgc                                                                                 >  1:1734724/1‑100 (MQ=255)
            ggTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGgc                                                                                 >  1:2260495/1‑100 (MQ=255)
            ggTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGgc                                                                                 <  2:771940/100‑1 (MQ=255)
             gTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCACCTGAGCCGATATTTCATCCCACAAGAATGTGgcg                                                                                >  2:720215/1‑100 (MQ=255)
                tACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTc                                                                             >  1:1005988/1‑100 (MQ=255)
                 aCTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAGGTTCGCAAGCGGGCCAGTCTCCTGAGCCCATATTTTATCCCACAAGATTGTGGCGCTcc                                                                            >  2:1537140/1‑100 (MQ=255)
                  cTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCcg                                                                           >  1:2559836/1‑100 (MQ=255)
                  cTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATACCACAAGAATGTGGCGCTCcg                                                                           >  1:18788/1‑100 (MQ=255)
                   tgtgGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCcgc                                                                          <  2:1584290/100‑1 (MQ=255)
                   tgtgGTAGAGTAACCGTGAAGACAAAATCTCTCTGAGCTGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCcgc                                                                          >  2:3457813/1‑100 (MQ=255)
                      ggTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGt                                                                       <  1:2251873/100‑1 (MQ=255)
                        tAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGCCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTg                                                                     <  2:252429/100‑1 (MQ=255)
                        tAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGACCAGTCCCCTGAGCCGATATTTCATCCCACAAGACTGAGGCGCTCCGCGGTTg                                                                     <  2:3886344/100‑1 (MQ=255)
                         agagTAGCCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTgg                                                                    <  1:2917703/100‑1 (MQ=255)
                         agagTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTgg                                                                    <  1:3189772/100‑1 (MQ=255)
                          gagTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGt                                                                   >  2:130989/1‑100 (MQ=255)
                          gagTAACCGTGAAGACAAAATTACTCTGAGAGGTTCACAAGCGCACCAGTCCCCTGAGCCCATACTTCACCCCCCAGGACTGTGACCCTCCGCCGTTGGt                                                                   >  2:1434167/1‑100 (MQ=255)
                           agTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTg                                                                  <  1:2189036/100‑1 (MQ=255)
                            gTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGa                                                                 >  2:327185/1‑100 (MQ=255)
                            gTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGa                                                                 <  2:1833641/100‑1 (MQ=255)
                            gTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGa                                                                 >  2:2410190/1‑100 (MQ=255)
                            gTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGa                                                                 >  1:2659646/1‑100 (MQ=255)
                            gTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATACCACAAGAATGTGGCGCTCCGCGGTTGGTGa                                                                 <  2:691981/100‑1 (MQ=255)
                               aCCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCa                                                              <  1:2110508/100‑1 (MQ=255)
                               aCCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCa                                                              <  1:3432285/100‑1 (MQ=255)
                               aCCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCa                                                              <  1:262762/100‑1 (MQ=255)
                               aCCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCa                                                              <  1:2205070/100‑1 (MQ=255)
                               aCCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCa                                                              <  2:307970/100‑1 (MQ=255)
                                ccGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCAt                                                             <  1:2371033/100‑1 (MQ=255)
                                  gTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGc                                                           >  1:3715408/1‑100 (MQ=255)
                                   tGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCt                                                          >  1:1435320/1‑100 (MQ=255)
                                    gAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCGGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTc                                                         <  1:3958929/100‑1 (MQ=255)
                                    gAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTc                                                         <  1:1932848/100‑1 (MQ=255)
                                     aaGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACCAGAATGTGGCGCTCCGCGGTTGGTGAGCCTGCTCg                                                        >  2:38601/1‑100 (MQ=255)
                                     aaGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCg                                                        <  1:133213/100‑1 (MQ=255)
                                      aGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCgg                                                       >  1:1747523/1‑100 (MQ=255)
                                      aGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCgg                                                       >  2:1760066/1‑100 (MQ=255)
                                        aCAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGgtc                                                     >  1:2080675/1‑100 (MQ=255)
                                        aCAAAATTTCTCTGAGATGGTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGgtc                                                     <  2:3167314/100‑1 (MQ=255)
                                         cAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGgtcc                                                    >  1:513891/1‑100 (MQ=255)
                                         cAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGgtcc                                                    >  2:2759133/1‑100 (MQ=255)
                                               ttCTCTGAGATGTTCGCAAGCGGGCCATTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCga                                              <  2:3736276/100‑1 (MQ=255)
                                                     gagaTGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGcc                                        >  1:1567002/1‑100 (MQ=255)
                                                     gagaTGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGcc                                        <  1:2406007/100‑1 (MQ=255)
                                                        aTGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATACCACAAGAATGTGGCGCGCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTa                                     >  1:824368/1‑100 (MQ=255)
                                                         tGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTaa                                    <  2:2520601/100‑1 (MQ=255)
                                                         tGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTaa                                    >  2:886276/1‑100 (MQ=255)
                                                         tGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATACCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTaa                                    >  2:49321/1‑100 (MQ=255)
                                                          gTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCGCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTaaa                                   >  2:3071429/1‑100 (MQ=255)
                                                          gTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGATGCCTTaaa                                   <  2:3836648/100‑1 (MQ=255)
                                                            tCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAAc                                 <  1:1047267/100‑1 (MQ=255)
                                                             cGCACGCTGGCAAGTCCCCCAAGCCCATCTTCCATCCCACAAGAATCTGACGCTCCGCGGTTGGTGAGCATGCCCGGTTCGTCCGAGAGACCTAAACACt                                <  2:58846/100‑1 (MQ=255)
                                                             cGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACt                                >  2:3799782/1‑100 (MQ=255)
                                                              ncAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTg                               >  1:463315/2‑100 (MQ=255)
                                                               cAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGc                              <  1:1138533/100‑1 (MQ=255)
                                                               cAAGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGc                              <  1:1162896/100‑1 (MQ=255)
                                                                aaGCGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGcg                             <  1:587599/100‑1 (MQ=255)
                                                                  gCGGGCCAGTCCCCGGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGcgac                           <  2:2302710/100‑1 (MQ=255)
                                                                   cGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGcgacg                          <  1:860102/100‑1 (MQ=255)
                                                                   cGGGCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGcgacg                          <  2:2788134/100‑1 (MQ=255)
                                                                    gggCCAGTCCCCTGAGCCGATATTTCATACCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGcgacga                         >  1:738823/1‑100 (MQ=255)
                                                                     ggCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCCTGCTCGGTCCGTCCGAGAAGCCTTCAAACTGCgacgac                        >  2:2746762/1‑100 (MQ=255)
                                                                     ggCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCgacgac                        >  2:1032612/1‑100 (MQ=255)
                                                                     ggCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCgacgac                        <  2:966582/100‑1 (MQ=255)
                                                                      gCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGaca                       <  1:1490817/100‑1 (MQ=255)
                                                                      gCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGaca                       <  1:2345594/100‑1 (MQ=255)
                                                                      gCCAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGaca                       <  1:385950/100‑1 (MQ=255)
                                                                      gCCAGTCCCCTGAGCCGATATTTCATACCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGaca                       >  2:302113/1‑100 (MQ=255)
                                                                       ccAGTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGacac                      >  2:3014592/1‑100 (MQ=255)
                                                                         aGTCCCCTGAGCCGATATTTCATCCCACAAGAATGCGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAGGCGTCACATCTGCGACGACACAt                    >  2:3150458/1‑100 (MQ=255)
                                                                          gTCCCCTGCGCCGATAATTCATACCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCCTGCTCTGGCCGTCCGAGAAGCCTTACAACTGCGACGAcacaat                   >  2:192553/1‑98 (MQ=255)
                                                                          gTCCCCTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACAtt                   >  1:4001287/1‑100 (MQ=255)
                                                                          gTCCCCTGAGCCGATAGTTCATACCACAAGAATGTGGCGCCCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGCAGCCTTAAACCTGCGACGACACAtt                   >  2:3280034/1‑100 (MQ=255)
                                                                            ccccTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCa                 <  1:2427950/100‑1 (MQ=255)
                                                                             cccTGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCAc                >  1:2847226/1‑100 (MQ=255)
                                                                              tcTGAGACGATAATTCATACCACAAGAATGCGGCGCTGCGCGGTTGGTGAGCATGCTCGTTAAGTCCGAGAATCCTTAAAACTGCGCTGTCACATTTAca               >  2:847285/2‑99 (MQ=255)
                                                                                tGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCtt             <  1:4070838/100‑1 (MQ=255)
                                                                                tGAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCtt             <  1:2717517/100‑1 (MQ=255)
                                                                                 gAGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCTTg            <  2:1188874/100‑1 (MQ=255)
                                                                                  aGCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCTTGa           >  1:949501/1‑100 (MQ=255)
                                                                                   gCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCTTGaa          >  1:2654916/1‑100 (MQ=255)
                                                                                   gCCGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCTTGaa          >  2:1174619/1‑100 (MQ=255)
                                                                                    ccGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCTTGAAc         >  2:3163539/1‑100 (MQ=255)
                                                                                     cGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCTTGAAcc        >  1:2493551/1‑100 (MQ=255)
                                                                                     cGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCTTGAAcc        <  1:2447875/100‑1 (MQ=255)
                                                                                     cGATATTTCATCCCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGGCCGAGAAGCCTGAAAACTGCGACGACACATTCACCTTGAAcc        >  2:3771788/1‑100 (MQ=255)
                                                                                      gATATTTCATACCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGACGCCTTAAAACTGCGACGACACATTCACCTTGAACca       >  2:3245540/1‑100 (MQ=255)
                                                                                                |                                                                                              
ATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATATTTCATACCACAAGAATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCTTGAACCAAGGGT  >  NC_000913/3055938‑3056128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: