Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,082,025 A→C Y38S (TAC→TCC)  loiP → Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,082,0250AC98.6% 493.6 / ‑5.6 146Y38S (TAC→TCC) loiPPhe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base C (74/69);  minor base T (1/1);  total (76/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

TATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGC  >  NC_000913/3081929‑3082121
                                                                                                |                                                                                                
tATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAg                                                                                               >  2:2988739/1‑100 (MQ=255)
  ttGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGtt                                                                                             <  1:726274/100‑1 (MQ=255)
    ggTAGCAATGAGCGTGGCAAGGGTACTAACTCGTTGCCAGACTATGGAATCCAAACGACTGCTCTCATCAGGCGCGGAAGCTTTTCAGGCTTTCAGttcg                                                                                           >  2:3985456/1‑98 (MQ=255)
    ggTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTg                                                                                           <  1:1170951/100‑1 (MQ=255)
    ggTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTg                                                                                           >  1:2034140/1‑100 (MQ=255)
     gTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGa                                                                                          <  1:1818581/100‑1 (MQ=255)
     gTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGa                                                                                          <  2:3474098/100‑1 (MQ=255)
     gTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGa                                                                                          >  1:297400/1‑100 (MQ=255)
      tAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGan                                                                                         <  1:3256222/100‑2 (MQ=255)
      tAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAg                                                                                         >  1:1318658/1‑100 (MQ=255)
      tAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAg                                                                                         <  1:2132315/100‑1 (MQ=255)
       aGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGt                                                                                        <  2:507466/100‑1 (MQ=255)
        gCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTg                                                                                       <  2:644001/100‑1 (MQ=255)
        gCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTg                                                                                       >  1:4070820/1‑100 (MQ=255)
        gCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTg                                                                                       <  2:2651852/100‑1 (MQ=255)
         cAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGa                                                                                      >  1:585884/1‑100 (MQ=255)
         cAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGa                                                                                      >  1:4086330/1‑100 (MQ=255)
         cAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGa                                                                                      <  1:947778/100‑1 (MQ=255)
          aaTGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGAt                                                                                     <  1:395730/100‑1 (MQ=255)
           aTGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATg                                                                                    >  1:109567/1‑100 (MQ=255)
            tGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATgc                                                                                   <  2:13391/100‑1 (MQ=255)
             gAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATgcg                                                                                  <  2:2223246/100‑1 (MQ=255)
              aGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATgcgc                                                                                 <  1:1566527/100‑1 (MQ=255)
               gCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGCGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCa                                                                                >  2:3858248/1‑100 (MQ=255)
               gCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCa                                                                                <  2:328465/100‑1 (MQ=255)
                   ggCAACGGTACTGCCTGGTTGCCAGCATATGGCCTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCGTCCAGTTTGAGTGATGCGCAGGTg                                                                            >  2:3472282/1‑100 (MQ=255)
                   ggCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTg                                                                            <  1:2545427/100‑1 (MQ=255)
                       aCGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGaaaa                                                                        >  2:3407309/1‑100 (MQ=255)
                        cGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCCGTTTGAGTGATGCGCAGGTGAAAAc                                                                       >  2:333788/1‑100 (MQ=255)
                        cGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAAc                                                                       <  1:1341465/100‑1 (MQ=255)
                        cGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAAc                                                                       <  1:3993963/100‑1 (MQ=255)
                         ggTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAAcc                                                                      <  2:3134110/100‑1 (MQ=255)
                             ctgactgGTTGCCAGAATATGGACTCCAACGGTCTGCTCTCATCAGGAGCGGGAGCTTTTCAGGCTGCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGa                                                                  >  2:806485/1‑100 (MQ=255)
                             ctgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGa                                                                  >  1:2438238/1‑100 (MQ=255)
                             ctgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGa                                                                  >  2:701913/1‑100 (MQ=255)
                             ctgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGa                                                                  >  1:354205/1‑100 (MQ=255)
                              ttactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTGATCAGGAGCTGAAGGTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAg                                                                 <  2:114287/98‑1 (MQ=255)
                              tgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAg                                                                 >  2:1261019/1‑100 (MQ=255)
                              tgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTGTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAg                                                                 <  2:483249/100‑1 (MQ=255)
                               gactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAga                                                                >  2:2717342/1‑99 (MQ=255)
                                actgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCg                                                               <  1:3501290/100‑1 (MQ=255)
                                 ctgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGa                                                              >  1:2776988/1‑100 (MQ=255)
                                 ctgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGa                                                              >  1:3685989/1‑100 (MQ=255)
                                   ggTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATc                                                            >  1:806130/1‑100 (MQ=255)
                                   ggTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCATGAGCGATc                                                            >  2:3797071/1‑100 (MQ=255)
                                    gTTGCCAGAATATGGACTCCGACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCa                                                           <  2:3568434/100‑1 (MQ=255)
                                     ttGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAg                                                          <  2:3616882/100‑1 (MQ=255)
                                     ttGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAg                                                          <  1:71988/100‑1 (MQ=255)
                                      tGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAgg                                                         >  2:529015/1‑100 (MQ=255)
                                      tGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTCTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAgg                                                         <  1:3677857/100‑1 (MQ=255)
                                       gCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGc                                                        <  2:2305227/100‑1 (MQ=255)
                                        ccAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCa                                                       >  2:2889092/1‑100 (MQ=255)
                                        ccAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCa                                                       >  1:2709490/1‑100 (MQ=255)
                                          aGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATg                                                     <  2:1696064/100‑1 (MQ=255)
                                          aGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATg                                                     <  2:2293307/100‑1 (MQ=255)
                                          aGAATATGGACGCCAACGGGCTGCTGTAATCAGGAGCGGAAGTTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATg                                                     <  2:1598506/100‑1 (MQ=255)
                                           gAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGt                                                    >  1:2293063/1‑100 (MQ=255)
                                             atatGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCa                                                  >  1:99842/1‑100 (MQ=255)
                                              tatGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAg                                                 >  2:3117564/1‑100 (MQ=255)
                                              tatGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAg                                                 <  1:3977244/100‑1 (MQ=255)
                                               atGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAgg                                                <  2:427685/100‑1 (MQ=255)
                                                tGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGga                                               <  2:2341165/100‑1 (MQ=255)
                                                 ggACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGgag                                              >  2:467033/1‑100 (MQ=255)
                                                  gACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGgaga                                             <  1:3368297/100‑1 (MQ=255)
                                                  gACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGgaga                                             >  2:2497980/1‑100 (MQ=255)
                                                   aCTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGAt                                            >  2:1125984/1‑100 (MQ=255)
                                                    cTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCCGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATg                                           >  2:753173/1‑100 (MQ=255)
                                                    cTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATg                                           >  1:3113268/1‑100 (MQ=255)
                                                    cTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATg                                           >  2:481273/1‑100 (MQ=255)
                                                     tCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATgg                                          <  2:2578616/100‑1 (MQ=255)
                                                      ccAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAGCCCTGAGCGATCAGGCATGTCAGGAGATGGa                                         <  1:3452882/100‑1 (MQ=255)
                                                        aaCGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACa                                       >  2:3653887/1‑100 (MQ=255)
                                                              cTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAgg                                 <  1:2004694/100‑1 (MQ=255)
                                                              cTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAgg                                 >  1:302963/1‑100 (MQ=255)
                                                                gCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGcg                               <  1:780446/100‑1 (MQ=255)
                                                                gCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGcg                               >  2:221185/1‑100 (MQ=255)
                                                                 ctctCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGcga                              >  1:1481300/1‑100 (MQ=255)
                                                                   ctcATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCATGCATGTCAGGAGATGGACAGCAAGGcgacg                            <  2:35862/100‑1 (MQ=255)
                                                                   ctcATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGcgacg                            >  2:1736428/1‑100 (MQ=255)
                                                                     catcaGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGAt                          >  1:603969/1‑100 (MQ=255)
                                                                     catcaGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGAt                          >  1:3052514/1‑100 (MQ=255)
                                                                     catcaGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGAt                          >  2:1722605/1‑100 (MQ=255)
                                                                       tcaGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTg                        <  1:2057738/100‑1 (MQ=255)
                                                                        caGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgc                       >  1:3331421/1‑100 (MQ=255)
                                                                         aGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgcg                      <  1:1272354/100‑1 (MQ=255)
                                                                             gCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgcccg                  >  2:3089210/1‑98 (MQ=255)
                                                                             gCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccag                  >  1:363610/1‑100 (MQ=255)
                                                                             gCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccag                  <  1:1722763/100‑1 (MQ=255)
                                                                              cGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTAAGGAGATGGACAGCAAGGCGACGATTGCgccagc                 <  2:3031038/100‑1 (MQ=255)
                                                                               ggAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagcc                <  2:1944157/100‑1 (MQ=255)
                                                                               ggAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagcc                <  1:4015031/100‑1 (MQ=255)
                                                                                gAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagcca               >  2:2129716/1‑100 (MQ=255)
                                                                                     ttttCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCGGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGc          >  1:4052615/1‑100 (MQ=255)
                                                                                     ttttCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGc          >  1:310043/1‑100 (MQ=255)
                                                                                        tCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGGGCGATCAGGGATGTCAGGAGATGGACAGCAAGGCGACGATGGCGCCAGCCAATAGCGaa       <  2:1703606/100‑1 (MQ=255)
                                                                                        tCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGaa       <  1:2933030/100‑1 (MQ=255)
                                                                                          aGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATa     >  2:1698248/1‑100 (MQ=255)
                                                                                           ggCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATAc    >  2:1779929/1‑100 (MQ=255)
                                                                                            gCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACg   >  2:1894151/1‑100 (MQ=255)
                                                                                             cTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGc  >  2:3214751/1‑100 (MQ=255)
                                                                                                |                                                                                                
TATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGC  >  NC_000913/3081929‑3082121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑