BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,170	T→G	V296G (GTC→GGC)	galP →	D‑galactose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,170	0	T	G	95.3%	484.4 / 9.7	149	V296G (GTC→GGC)	galP	D‑galactose transporter
Reads supporting (aligned to +/- strand): ref base T (5/2); new base G (69/73); total (74/75)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 150 total aligned reads displayed.

TTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATG  >  NC_000913/3089073‑3089264

ttCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGAGACCGTGATTGGCg                                                                                              <  1:1581748/100‑1 (MQ=255)

 tCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCgg                                                                                             <  1:690680/100‑1 (MQ=255)

  cACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTggcggc                                                                                            <  2:493181/100‑1 (MQ=255)

   aCCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGcc                                                                                           <  1:940095/100‑1 (MQ=255)

   aCCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGcc                                                                                           <  1:1073562/100‑1 (MQ=255)

   aCCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGcc                                                                                           >  1:785703/1‑100 (MQ=255)

    ccGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCt                                                                                          >  1:2673316/1‑100 (MQ=255)

    ccGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCt                                                                                          >  1:3046439/1‑100 (MQ=255)

       ggATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGAc                                                                                       <  1:1359692/100‑1 (MQ=255)

           gAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACTAACACTACCGAGCAAAGGTGGGGGAGCGTGATTGGCGGCCTGACAAAc                                                                                   >  2:2135329/1‑100 (MQ=255)

           gAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAAc                                                                                   <  2:2648896/100‑1 (MQ=255)

            aaCGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACg                                                                                  >  1:965523/1‑100 (MQ=255)

             aCGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGt                                                                                 >  2:2587375/1‑100 (MQ=255)

                tcatcaTGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACt                                                                              <  1:1603558/100‑1 (MQ=255)

                tcatcaTGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACt                                                                              <  1:2831633/100‑1 (MQ=255)

                tcatcaTGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACt                                                                              <  1:1548446/100‑1 (MQ=255)

                  atcatGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTg                                                                            >  1:1030311/1‑100 (MQ=255)

                  atcatGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTg                                                                            >  1:2597547/1‑100 (MQ=255)

                    catGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGcc                                                                          >  2:3887120/1‑100 (MQ=255)

                     atGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCa                                                                         >  1:168977/1‑100 (MQ=255)

                       gTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCAcc                                                                       >  1:110103/1‑100 (MQ=255)

                       gTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCAcc                                                                       >  2:2547694/1‑100 (MQ=255)

                        tATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCt                                                                      >  2:1855723/1‑100 (MQ=255)

                        tATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCt                                                                      >  1:235338/1‑100 (MQ=255)

                        tATTACACGCCGAAAATCCTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCt                                                                      >  1:3399536/1‑100 (MQ=255)

                         aTTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCtt                                                                     >  1:3740721/1‑100 (MQ=255)

                           tACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTa                                                                   >  1:2604826/1‑100 (MQ=255)

                            aCGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTAt                                                                  >  2:1415701/1‑100 (MQ=255)

                            aCGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTAt                                                                  >  1:3390316/1‑100 (MQ=255)

                             ctcgcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATc                                                                 <  1:494543/98‑1 (MQ=255)

                             cgcgCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATc                                                                 <  2:1314847/100‑1 (MQ=255)

                              gcgcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCg                                                                <  1:2687251/100‑1 (MQ=255)

                              gcgcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCg                                                                >  1:1704049/1‑100 (MQ=255)

                              gcgcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCg                                                                <  1:174975/100‑1 (MQ=255)

                               cgcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGAATGGCGGCCTGACCAACGTACTTGCCACCTTTATCGc                                                               >  1:3838419/1‑100 (MQ=255)

                                gcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCa                                                              <  1:224041/100‑1 (MQ=255)

                                 ccGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCaa                                                             >  1:1396203/1‑100 (MQ=255)

                                  cGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAAt                                                            >  1:2883666/1‑100 (MQ=255)

                                         cTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCtt                                                     >  2:1222402/1‑100 (MQ=255)

                                          ttCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttg                                                    <  1:1233997/100‑1 (MQ=255)

                                          ttCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttg                                                    >  2:3046077/1‑100 (MQ=255)

                                          ttCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttg                                                    >  1:2975652/1‑100 (MQ=255)

                                             gagcTGGCGGGTTATACCAACACTACCGAGCATAGGGGGGGGACCTTGATTGGCGGCCTGACGAACGTACTTGCCAGCTTTATCGCAATCGGCCttgttg                                                 <  2:3203519/97‑1 (MQ=255)

                                             gAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttgttg                                                 >  2:3303617/1‑100 (MQ=255)

                                               aCTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGAc                                               <  1:688322/100‑1 (MQ=255)

                                                cTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGAcc                                              >  2:266317/1‑100 (MQ=255)

                                                cTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGAcc                                              >  1:1528757/1‑100 (MQ=255)

                                                  ggCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGc                                            >  1:1319660/1‑100 (MQ=255)

                                                    cGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTg                                          <  2:2840291/100‑1 (MQ=255)

                                                     gggTTATACCAACACGCCCGAGCGAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTgg                                         <  2:844305/100‑1 (MQ=255)

                                                      ggTTATACCAACGCTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTggt                                        <  2:286504/100‑2 (MQ=255)

                                                        ttATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGa                                      <  1:3214817/100‑1 (MQ=255)

                                                        ttATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGa                                      <  1:220336/100‑1 (MQ=255)

                                                         tataCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGAc                                     <  2:769220/100‑1 (MQ=255)

                                                          ataCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACg                                    <  1:3927593/100‑1 (MQ=255)

                                                          ataCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGCTGACCGCTGGGGACg                                    >  2:2184375/1‑100 (MQ=255)

                                                           taCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGt                                   >  2:219870/1‑100 (MQ=255)

                                                            aCCAACACTACCGAGCAAATGTGGGGGACCGTTATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTa                                  <  2:2177190/100‑1 (MQ=255)

                                                             ccAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTaa                                 >  1:3652114/1‑100 (MQ=255)

                                                             ccAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTaa                                 >  2:2471445/1‑100 (MQ=255)

                                                              cAACACTACCGAGCAAATGTGGGGTACCGTCATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTaaa                                <  2:2310501/100‑1 (MQ=255)

                                                              cAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTaaa                                <  1:3103906/100‑1 (MQ=255)

                                                               aaCACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAc                               >  2:906941/1‑100 (MQ=255)

                                                                acacTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTGTCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAcc                              <  2:3911200/100‑1 (MQ=255)

                                                                acacTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAcc                              <  1:1359919/100‑1 (MQ=255)

                                                                acacTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAcc                              >  1:1947147/1‑100 (MQ=255)

                                                                acacTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAcc                              <  1:3043724/100‑1 (MQ=255)

                                                                acacTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAcc                              <  2:2428266/100‑1 (MQ=255)

                                                                  acgaCCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaa                            <  2:2357724/97‑1 (MQ=255)

                                                                   cTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaac                           >  2:623998/1‑100 (MQ=255)

                                                                     aCCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgc                         <  2:3494384/100‑1 (MQ=255)

                                                                     aCCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgc                         >  1:3925764/1‑100 (MQ=255)

                                                                      ccGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgct                        >  1:1759469/1‑100 (MQ=255)

                                                                       cGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgcta                       >  1:4063222/1‑100 (MQ=255)

                                                                        gAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaa                      >  1:1825075/1‑100 (MQ=255)

                                                                         aGCAAATGTGGGGGACTGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaac                     <  1:1623794/100‑1 (MQ=255)

                                                                         aGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaac                     <  2:2024489/100‑1 (MQ=255)

                                                                          gCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCCGGGGACGTAAACCaacgctaacg                    >  2:3694367/1‑100 (MQ=255)

                                                                          gCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaacg                    <  2:3375716/100‑1 (MQ=255)

                                                                           cAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaacgc                   <  1:3683341/100‑1 (MQ=255)

                                                                           cAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAAGCGGCCTTGTTGACCGCTGGGGACGTAAACCagcgctaacgc                   <  2:1475665/100‑1 (MQ=255)

                                                                            aaaTGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaacgct                  <  1:2834432/100‑1 (MQ=255)

                                                                            aaaTGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaacgct                  <  1:810606/100‑1 (MQ=255)

                                                                             aaTGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTg                 >  2:2898012/1‑100 (MQ=255)

                                                                             aaTGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCCGGGGACGTAAACCAACGCTAACGCTg                 >  2:182710/1‑100 (MQ=255)

                                                                               tgtgGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTggg               <  1:1838151/100‑1 (MQ=255)

                                                                                 tgGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCt             >  2:1665958/1‑100 (MQ=255)

                                                                                  gggggACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCtt            >  2:1900172/1‑100 (MQ=255)

                                                                                   ggggACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTc           <  2:3561528/100‑1 (MQ=255)

                                                                                    nggACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGATGGGCttgc          >  1:3260973/2‑98 (MQ=255)

                                                                                       aCCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTgg       >  1:3136145/1‑100 (MQ=255)

                                                                                        ccGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGt      >  1:228201/1‑100 (MQ=255)

                                                                                        ccGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGt      >  2:3826310/1‑100 (MQ=255)

                                                                                        ccGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGt      <  1:1885951/100‑1 (MQ=255)

                                                                                        ccGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGt      >  1:2482198/1‑100 (MQ=255)

                                                                                        ccGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTGCCTGGt      >  2:1595470/1‑100 (MQ=255)

                                                                                          gTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGa    >  2:3968620/1‑100 (MQ=255)

                                                                                          gTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGa    >  1:1208279/1‑100 (MQ=255)

                                                                                           tGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGAt   <  1:2474504/100‑1 (MQ=255)

                                                                                            gATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATg  >  2:3367566/1‑100 (MQ=255)

TTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATG  >  NC_000913/3089073‑3089264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`