Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,101,594 T→G A10A (GCA→GCC)  yggN ← DUF2884 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,101,5940TG98.6% 493.8 / NA 148A10A (GCA→GCC) yggNDUF2884 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base G (75/69);  minor base C (1/1);  total (76/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

CCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGT  >  NC_000913/3101497‑3101688
                                                                                                 |                                                                                              
ccTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGggc                                                                                              <  2:752956/100‑1 (MQ=255)
  ttCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGggctg                                                                                            <  2:17653/100‑1 (MQ=255)
  ttCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGggctg                                                                                            >  2:613373/1‑100 (MQ=255)
  ttCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGggctg                                                                                            >  2:505046/1‑100 (MQ=255)
   tCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGggctgc                                                                                           >  1:1982926/1‑100 (MQ=255)
    cACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgcc                                                                                          >  2:3606767/1‑100 (MQ=255)
      ccTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgccgc                                                                                        >  2:2454085/1‑100 (MQ=255)
       cTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgccgcc                                                                                       <  1:1416352/100‑1 (MQ=255)
       cTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTgccgcc                                                                                       <  2:594331/100‑1 (MQ=255)
        tGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCca                                                                                      >  1:597686/1‑100 (MQ=255)
        tGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCca                                                                                      >  1:480241/1‑100 (MQ=255)
         ncACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcag                                                                                     >  1:3261516/2‑100 (MQ=255)
         gCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcag                                                                                     <  2:2189361/100‑1 (MQ=255)
          cACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcagc                                                                                    <  1:2040824/100‑1 (MQ=255)
            cGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcagcag                                                                                  >  2:1013274/1‑100 (MQ=255)
            cGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCcagcag                                                                                  >  2:3737742/1‑100 (MQ=255)
                ttGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCAtt                                                                              >  2:3797272/1‑100 (MQ=255)
                 tGCCGGCTTACAAGCACATCGTCACGCGGCGTGACGATTCACTGGTAGTCGGCGTGAACGGTCATTGCCTTTACCGAACGGGCTGCCGCCAGCAGCAttt                                                                             >  2:534412/1‑100 (MQ=255)
                   cGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTg                                                                           >  1:3843895/1‑100 (MQ=255)
                     ggCTGACAATCACATCGTCACGCGGCGTTACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTgcg                                                                         <  2:1730230/100‑1 (MQ=255)
                     ggCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTgcg                                                                         <  1:3522768/100‑1 (MQ=255)
                     ggCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTgcg                                                                         <  1:2763471/100‑1 (MQ=255)
                      gCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTgcgc                                                                        <  1:471509/100‑1 (MQ=255)
                       cTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGca                                                                       <  1:2995091/100‑1 (MQ=255)
                        tGACAATCACATCGTCATGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGcat                                                                      >  2:2383057/1‑100 (MQ=255)
                         naCAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGcatc                                                                     >  1:166144/2‑100 (MQ=255)
                         gACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGcatc                                                                     <  2:3471357/100‑1 (MQ=255)
                         gACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGcatc                                                                     >  1:2135262/1‑100 (MQ=255)
                           cAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGcatcat                                                                   >  1:2385911/1‑100 (MQ=255)
                            aaTCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGcatcatn                                                                  <  1:3249947/100‑2 (MQ=255)
                             aTCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAg                                                                 <  1:1596798/100‑1 (MQ=255)
                               cacaTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTc                                                               >  1:1471377/1‑100 (MQ=255)
                               cacaTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTc                                                               >  1:376196/1‑100 (MQ=255)
                                gcagcGTCACGCCGCGTTACGCTGCACTGGTACTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCt                                                              >  1:1210449/5‑100 (MQ=255)
                                gcagcGTCACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCt                                                              >  1:961982/5‑100 (MQ=255)
                                acaTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCt                                                              >  2:1963664/1‑100 (MQ=255)
                                 cagcGTCACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCtt                                                             >  1:2550624/4‑100 (MQ=255)
                                 cagcGTCACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCtt                                                             >  1:749756/4‑100 (MQ=255)
                                    cGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTccc                                                          <  1:1288495/100‑1 (MQ=255)
                                       cACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCa                                                       <  1:2910789/100‑1 (MQ=255)
                                       cACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCa                                                       <  1:918336/100‑1 (MQ=255)
                                         cgcgGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAg                                                     >  1:1550775/1‑100 (MQ=255)
                                          gcggcgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGa                                                    <  1:3322587/100‑1 (MQ=255)
                                           cggcgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaa                                                   <  2:2485119/100‑1 (MQ=255)
                                              cgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaaaaa                                                <  1:2162729/100‑1 (MQ=255)
                                               gTGACGCTGCTCTGGTAGTCGGCGTCAGCGGTCATTGCCGCCCCTCAAAGCGCTACCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGcacaaa                                               >  2:652780/1‑100 (MQ=255)
                                               gTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaaaaaa                                               <  1:295471/100‑1 (MQ=255)
                                                   cGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAg                                           <  1:514328/100‑1 (MQ=255)
                                                    gCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAgg                                          <  1:610232/100‑1 (MQ=255)
                                                     cTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGc                                         >  2:81351/1‑100 (MQ=255)
                                                      tGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGcc                                        <  2:3589794/100‑1 (MQ=255)
                                                       gCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCa                                       >  2:2166305/1‑100 (MQ=255)
                                                        cACTGGTAGTCGGCATGAGCGGTCATTGCCGCCACAGAGAGAGCTGCCGCCACCAGCATTTTCCGCCTCATGGCCTTCCGTAAAGCAAAAGTCCGGCCAg                                      <  2:2156838/100‑1 (MQ=255)
                                                        cACTGGTAGTCGACGGGAGCGGTCAGTGCCGTCACTGAAGGGGCTGCGGCCAGCCGCATTTTGCGCATCATAGACTTAACAAAAAAAAAAATCAGGCCAg                                      <  2:2872342/100‑1 (MQ=255)
                                                         aCTGGTAGTCGGCGTGAGCGGTCATTCCCGTCCCCGAATGGGCTGCCGCTAGCAGCCTTTTCCTCTACATCGTCTTCCCTCCCGTAAAATTCAGACCAGc                                     >  2:2350784/1‑100 (MQ=255)
                                                          cTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCa                                    >  2:1288183/1‑100 (MQ=255)
                                                          cTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCa                                    >  2:723720/1‑100 (MQ=255)
                                                            ggTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAtt                                  >  2:3283376/1‑100 (MQ=255)
                                                              tAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTg                                <  1:928587/100‑1 (MQ=255)
                                                              tAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTg                                >  1:836627/1‑100 (MQ=255)
                                                               aGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCaaatgc                               <  1:1161702/100‑6 (MQ=255)
                                                               aGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGc                               >  2:583961/1‑100 (MQ=255)
                                                               aGTCGGCGTGAGCGGTCATTGCCGGCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTACCCTCAAGAAAAAATCAGGCCAGCATTTGc                               <  1:1831694/100‑1 (MQ=255)
                                                                 tCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTg                             <  2:1429648/100‑1 (MQ=255)
                                                                      gTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGcccc                        >  1:389789/1‑100 (MQ=255)
                                                                      gTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGcccc                        >  1:1229057/1‑100 (MQ=255)
                                                                      gTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAAATGCTGGcctg                        <  2:3871026/100‑3 (MQ=255)
                                                                       tGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCa                       >  1:864978/1‑100 (MQ=255)
                                                                         aGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAAATGCTGGcctgatt                     <  1:3048438/100‑6 (MQ=255)
                                                                          gCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCCGAt                    >  2:3035419/1‑100 (MQ=255)
                                                                           cGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAtt                   >  1:2827395/1‑100 (MQ=255)
                                                                           cGGTCATCGCCGTCAATGAAAGGGCTCCCGACAGCAGCATTTTGCGCTTCTTCGTCTAAAAAAAAAAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAtt                   <  2:3951575/100‑1 (MQ=255)
                                                                             gTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGa                 <  1:360438/100‑1 (MQ=255)
                                                                             gTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGG‑CCCAGATTGAt                <  2:899609/100‑1 (MQ=255)
                                                                             gTCATAGCCGACACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTAAAAAAAAAAAAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGa                 <  2:1654224/100‑1 (MQ=255)
                                                                               cATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATa               >  2:3119588/1‑100 (MQ=255)
                                                                               cATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATa               <  1:1367794/100‑1 (MQ=255)
                                                                                 ttGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAAc             <  1:1039981/100‑1 (MQ=255)
                                                                                 ttGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAAc             <  1:4073551/100‑1 (MQ=255)
                                                                                 ttGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAAc             <  1:4073929/100‑1 (MQ=255)
                                                                                  tGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCACGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGCTAACa            <  2:848132/100‑1 (MQ=255)
                                                                                  tGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATCTGTCGCATCATAGTCTTCCCTCAAGAAAACATCAGGCCAGCCTTTGCTGGCCCCAGATTGATAACa            <  2:1578386/100‑1 (MQ=255)
                                                                                   gCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa           <  1:3163540/100‑1 (MQ=255)
                                                                                   gCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa           >  1:3444982/1‑100 (MQ=255)
                                                                                    ctgTCACTGCAAGGGCTGCCGCCAGGAGCATTTTGCGCATCATAGGCCCCCCTCAAGCAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATATCaaa          <  2:294771/98‑1 (MQ=255)
                                                                                    ccGTCACTGAAAGCGCGGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaaa          <  1:4078484/100‑1 (MQ=255)
                                                                                     cGTCACTGCACGGGCTGCAGCACGCAGCCTTTTGAGCATCATAGTCTTCCCTCAAGCAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACACAg         <  2:3210504/100‑1 (MQ=255)
                                                                                     cGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg         >  1:39006/1‑100 (MQ=255)
                                                                                     cGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg         >  2:553933/1‑100 (MQ=255)
                                                                                     cGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg         <  1:2612609/100‑1 (MQ=255)
                                                                                      gTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGt        >  1:3706859/1‑100 (MQ=255)
                                                                                        ctcTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc      <  2:3183592/98‑1 (MQ=255)
                                                                                        cACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc      >  2:2725823/1‑100 (MQ=255)
                                                                                         aCTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcg     <  1:2354711/100‑1 (MQ=255)
                                                                                          cTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgc    >  1:44138/1‑100 (MQ=255)
                                                                                          cTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgc    >  1:3635096/1‑100 (MQ=255)
                                                                                           tGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg   <  1:3339929/100‑1 (MQ=255)
                                                                                           tGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg   <  1:1073904/100‑1 (MQ=255)
                                                                                           tGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg   <  2:2102309/100‑1 (MQ=255)
                                                                                            gAAAGGGCTGCCGCCAGCAGCATTTAGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGt  >  1:527901/1‑100 (MQ=255)
                                                                                                 |                                                                                              
CCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGT  >  NC_000913/3101497‑3101688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑