Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,105,513 T→G intergenic (+1/‑201) mltC → / → nupG membrane‑bound lytic murein transglycosylase C/nucleoside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,105,5130TG91.6% 370.6 / 19.9 132intergenic (+1/‑201)mltC/nupGmembrane‑bound lytic murein transglycosylase C/nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base T (5/6);  new base G (55/65);  total (60/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 132 total aligned reads displayed.

GTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACA  >  NC_000913/3105417‑3105609
                                                                                                |                                                                                                
gTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTcc                                                                                               >  1:3996593/1‑100 (MQ=255)
gTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTcc                                                                                               <  1:3999138/100‑1 (MQ=255)
gTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTcc                                                                                               <  1:4023786/100‑1 (MQ=255)
gTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAAAAAAAAAAAAAAATCCTACCGCCGCCGATAAGTcc                                                                                               <  2:1032624/100‑1 (MQ=255)
 tttATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCa                                                                                              >  1:2827894/1‑100 (MQ=255)
 tttATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCa                                                                                              >  2:222325/1‑100 (MQ=255)
  ttATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCAt                                                                                             <  1:2458381/100‑1 (MQ=255)
   tATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCAtt                                                                                            <  1:2504616/100‑1 (MQ=255)
   tATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCAtt                                                                                            >  2:3103518/1‑100 (MQ=255)
   tATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCAtt                                                                                            >  1:2015646/1‑100 (MQ=255)
    aTCAGACGCTGACGACCCGCCATCCCTCTGCGTAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTa                                                                                           <  2:1242684/100‑1 (MQ=255)
    aTCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTa                                                                                           <  2:1037013/100‑1 (MQ=255)
    aTCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTa                                                                                           <  2:521802/100‑1 (MQ=255)
    aTCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTa                                                                                           >  1:2453623/1‑100 (MQ=255)
     tCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTaa                                                                                          <  1:415513/100‑1 (MQ=255)
     tCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTaa                                                                                          >  2:2628778/1‑100 (MQ=255)
     tCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTaa                                                                                          >  1:3138903/1‑100 (MQ=255)
       aGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAAcc                                                                                        <  1:305977/100‑1 (MQ=255)
       aGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAAcc                                                                                        <  2:961457/100‑1 (MQ=255)
        gACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCg                                                                                       <  1:1049149/100‑1 (MQ=255)
         aCGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGc                                                                                      >  1:1017803/1‑100 (MQ=255)
         aCGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGc                                                                                      <  2:619697/100‑1 (MQ=255)
           gCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGccc                                                                                    <  1:325019/100‑1 (MQ=255)
            cTGACGACCCGCCATCCCTCTGCGGATTCTCGCCGTTATCTTTATAAAACGAATTCCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGcccc                                                                                   <  2:3546562/100‑1 (MQ=255)
            cTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGcccc                                                                                   <  1:3976901/100‑1 (MQ=255)
             tGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCt                                                                                  <  2:1543425/100‑1 (MQ=255)
             tGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCt                                                                                  <  1:427506/100‑1 (MQ=255)
             tGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCt                                                                                  >  1:1909295/1‑100 (MQ=255)
              gacgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTg                                                                                 >  1:1856377/1‑100 (MQ=255)
                cgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAACATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGAc                                                                               <  1:1838369/100‑1 (MQ=255)
                cgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGAc                                                                               <  1:1870109/100‑1 (MQ=255)
                cgacCCCCCATCCCTCTGCGGAATCTCGCCGTGATCTTTATAAAGGGAATGCCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGAc                                                                               <  2:2347039/100‑1 (MQ=255)
                  acCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGa                                                                             <  2:2653364/100‑1 (MQ=255)
                      gCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCt                                                                         >  1:564453/1‑100 (MQ=255)
                          tCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAgg                                                                     >  1:2510187/1‑100 (MQ=255)
                          tCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAgg                                                                     <  2:1348617/100‑1 (MQ=255)
                            ccTCTGCGGAATCTCGGCGGTATCGTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCAATAAGTCCATTAACCGCCCCTGACGATTCTCAgggg                                                                   >  2:1698374/1‑100 (MQ=255)
                            ccTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAgggg                                                                   <  2:2820106/100‑1 (MQ=255)
                            ccTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAgggg                                                                   >  1:79255/1‑100 (MQ=255)
                               ctGCGGAAGCGCGCTGGGATCTTTATAAAGTGACTCCCGCGCATAGATCCTACCGCCGCCGATAAGTCCATGAACCGCCCCGGACGATGCTCAGGGGCaa                                                                <  2:3653177/100‑1 (MQ=255)
                                   ggAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAAt                                                            >  1:2613620/1‑100 (MQ=255)
                                   ggAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAAt                                                            >  1:2614489/1‑100 (MQ=255)
                                    gAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATg                                                           <  1:431823/100‑1 (MQ=255)
                                    gAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATACTCATGGGCAAAAATg                                                           <  2:3875782/100‑1 (MQ=255)
                                     aaTCTCGCCGTTATCTTTATAAAGTGAATACCGCGCACAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGt                                                          <  2:1984092/100‑1 (MQ=255)
                                       tctcGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTa                                                        <  2:1985689/100‑1 (MQ=255)
                                        ctcGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTAt                                                       >  2:2130764/1‑100 (MQ=255)
                                         tcGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATc                                                      >  1:2110854/1‑100 (MQ=255)
                                         ctgCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATc                                                      <  1:2189395/98‑1 (MQ=255)
                                          cGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCTCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATcc                                                     >  2:2251334/1‑100 (MQ=255)
                                          cGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATcc                                                     <  2:1717870/100‑1 (MQ=255)
                                          cGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATcc                                                     <  2:2471583/100‑1 (MQ=255)
                                          cGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATcc                                                     <  1:1877515/100‑1 (MQ=255)
                                           gCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATcag                                                    >  2:2155738/1‑98 (MQ=255)
                                              gTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACAt                                                 >  2:1122337/1‑100 (MQ=255)
                                                 aTCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATcac                                              <  2:1806822/100‑1 (MQ=255)
                                                 aTCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATCAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATcac                                              <  2:1967036/100‑1 (MQ=255)
                                                  tCTTTATAAAGTGAATACCGCGCAAAAGTCCTACCGCCGCCGATAAGTGCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATcaga                                             >  2:3978459/1‑98 (MQ=255)
                                                    tttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAAt                                           >  1:813511/1‑100 (MQ=255)
                                                    tttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAAt                                           >  2:506372/1‑100 (MQ=255)
                                                        taAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCg                                       <  2:741577/100‑1 (MQ=255)
                                                         aaaGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGt                                      >  1:368081/1‑100 (MQ=255)
                                                         aaaGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGt                                      >  1:2509358/1‑100 (MQ=255)
                                                           agggAATACCGCGGAAAAACCCTACCGCCGCAGATAATTCCATTAACCGCCCCTGACGATGAAAAGAGAAAAAAATGTTATCCACATCACAATTTCGttt                                    <  2:2161005/97‑1 (MQ=255)
                                                           aGTGAATACCGCGCAAAAATCCTAACGCCACCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTAATCCACATCACAATTTCGttt                                    >  1:3805163/1‑100 (MQ=255)
                                                            gTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGtttt                                   >  1:1189695/1‑100 (MQ=255)
                                                             tGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTg                                  <  2:611362/100‑1 (MQ=255)
                                                             tGAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTg                                  <  1:3313923/100‑1 (MQ=255)
                                                              gAATACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGc                                 >  2:618222/1‑100 (MQ=255)
                                                                aTACCGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCaa                               >  1:364913/1‑100 (MQ=255)
                                                                   ccGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAAtt                            >  1:4048137/1‑100 (MQ=255)
                                                                   ccGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAAtt                            >  1:58986/1‑100 (MQ=255)
                                                                   ccGCGCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAAtt                            >  1:1559892/1‑100 (MQ=255)
                                                                    cgcgCAAAACTCCTACCGCCGCCGAGAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTg                           <  2:2735771/100‑1 (MQ=255)
                                                                    cgcgCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTg                           >  2:1200575/1‑100 (MQ=255)
                                                                    cgcgCAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTg                           <  1:1377291/100‑1 (MQ=255)
                                                                     gtgcAAAAATCCCACCGCCCCCGATAAGTCCAATAACCGCCCCTGACGATCCTAAGGGGCAAAATTGTTATCCACATCACAATTTCGTTTTGCAAATTgg                          <  2:2589363/98‑1 (MQ=255)
                                                                     gcgcAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAACTTgg                          >  2:3160612/1‑100 (MQ=255)
                                                                     gcgcAAAAACCCTACCGCCGACGATAAGTCCATTAACCGCCCCTGACGATGAAAAAAAAAAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTgg                          <  2:812857/100‑1 (MQ=255)
                                                                      cgcAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTggg                         >  1:913454/1‑100 (MQ=255)
                                                                        cAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGaa                       <  1:586843/100‑1 (MQ=255)
                                                                        cAAAAATCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGaa                       >  1:40227/1‑100 (MQ=255)
                                                                          aaaaTCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTCACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATg                     >  2:3076696/1‑100 (MQ=255)
                                                                           aaaTCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGt                    <  1:2381968/100‑1 (MQ=255)
                                                                              tcctccCGCCGCCGAGAAGTCCATTATCCGCCCCTGACGATGATAAGGAGAAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTg                 <  2:1576982/100‑1 (MQ=255)
                                                                              tCCTACCGCCGCCGATAAGTCCATTAACCGCCCCTTACGAAGCTCCCGGGCAAAAATGTTATCCACATCACAATTTCGTTTTCCAAATTGGGAATGTTTg                 >  2:3321057/1‑100 (MQ=255)
                                                                                cTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCTCAATTTCGTTTTGCAAATTGGGAATGTTTGCa               >  2:128969/1‑100 (MQ=255)
                                                                                cTACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCa               <  1:2724434/100‑1 (MQ=255)
                                                                                 tACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCaa              >  1:3360295/1‑100 (MQ=255)
                                                                                 tACCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCaa              >  2:3377973/1‑100 (MQ=255)
                                                                                  aCCGCCGCCGATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAat             >  2:3500454/1‑100 (MQ=255)
                                                                                   ccgccgccgATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAatt            <  1:2611042/100‑1 (MQ=255)
                                                                                     gccgccgATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAattat          >  1:1490015/1‑100 (MQ=255)
                                                                                      ccgccgATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAattatt         <  2:1726616/100‑1 (MQ=255)
                                                                                       cgccgATAAGTCCATTATCCGCGCCTGACGAGGCTTATGGGCAAAAGTGTTATCCACATCACAATTTCGTTTTGCAAAGTGGGAATGTTTGCAATTAttt        <  2:2361144/100‑1 (MQ=255)
                                                                                       cgccgATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTAttt        <  1:1892369/100‑1 (MQ=255)
                                                                                         ccgATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGc      >  2:1749556/1‑100 (MQ=255)
                                                                                          cgATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGcc     <  1:2135128/100‑1 (MQ=255)
                                                                                          cgATAAGTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGcc     <  2:1540527/100‑1 (MQ=255)
                                                                                             tAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCcaca  <  1:655537/100‑1 (MQ=255)
                                                                                                |                                                                                                
GTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACA  >  NC_000913/3105417‑3105609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑